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| 1 | Effect of in vitro interferon-beta administration on hepatitis C virus in peripheral blood mononuclear cells as a predictive marker of clinical response to interferon treatment for chronic hepatitis C显示文摘AIM:To test whether in vitro incubation of peripheral blood mononuclear cells (PBMC) with interferon (IFN) could efficiently decrease hepatitis C virus-RNA (HCV-RNA) amount and to analyze whether this effect was associated with clinical response to IFN.METHODS:Twenty-seven patients with histologically proven chronic hepatitis C were given intravenous administration of 6 million units (MU) IFN-β daily for 6 weeks followed by three times weekly for 20 weeks. PBMC collected before IFN therapy were incubated with IFN-β and HCV-RNA in PMBC was semi-quantitatively determined.RESULTS: Twenty-five patients completed IFN therapy.Eight patients (32%) had sustained loss of serum HCV-RNA with normal serum ALT levels after IFN therapy (complete responders).HCV-RNA in PBMC was detected in all patients,whereas it was not detected in PBMC from healthy subjects.In vitro administration of IFN-β decreased the amount of HCV-RNA in PMBC in 18 patients (72%). Eight of these patients obtained complete response. On the other hand,none of the patients whose HCV-RNA in PBMC did not decrease by IFN-β was complete responders. Multiple logistic regression analysis revealed that the decrease of HCV-RNA amount in PBMC by IFN-β was the only independent predictor for complete response (P<0.05).CONCLUSION:The effect of in vitro IFN-β on HCV in PBMC reflects clinical response and would be taken into account as a predictive marker of IFN therapy for chronic hepatitis C. | Kaori Mochizuki Tatehiro Kagawa Shinji Takashimizu Kazuya Kawazoe Sei-Ichiro Kojima Naruhiko Nagata Atsushi Nakano Yasuhiro Nishizaki Koichi Shiraishi Masaru Itakura Norihito Watanabe Tetsuya Mine Shohei Matsuzaki | 2004 | World Journal of Gastroenterology2004，10，5： | 12 |
| 2 | Fatal liver failure due to reactivation of lamivudine-resistant HBV mutant显示文摘We report a case of fatal liver failure due to reactivation of lamivudine-resistant HBV. A 53-year-old man was followed since 1998 for HBV-related chronic hepatitis. Serum HBV-DNA was 150 MEq/mL (branched DNA signal amplification assay) and ALT levels fluctuated between 50-200 IU/L with no clinical signs of liver cirrhosis. Lamivudine (100 mg/d) was started in May 2001 and serum HBV-DNA subsequently decreased below undetectable levels. In May 2002, serum HBV-DNA had increased to 410 MEq/mL, along with ALT flare (226 IU/L). The YMDD motif in the DNA polymerase gene had been replaced by YIDD. Lamivudine was continued and ALT spontaneously decreased to the former levels. On Oct 3 the patient presenting with general fatigue, nausea and jaundice was admitted to our hospital. The laboratory data revealed HBV reactivation and liver failure (ALT: 1828 IU/L, total bilirubin: 10 mg/dL, and prothrombin INR: 3.24). For religious reasons, the patient and his family refused blood transfusion, plasma exchange and liver transplantation. The patient died 10 d after admission. The autopsy revealed remarkable liver atrophy. | Tatehiro Kagawa Norihito Watanabe Hisashi Kanouda Ichiro Takayama Tadahiko Shiba Takashi Kanai Kazuya Kawazoe Shinji Takashimizu Nobue Kumaki Kazuo Shimamura Shohei Matsuzaki Tetsuya Mine | 2004 | World Journal of Gastroenterology2004，10，11： | 10 |
| 3 | Improvem ent in atrophic gastritis and intetinal metaplasia in patients in whom Helicobacter pylori was eradicated显示文摘 | Ohkusa T Fujiki K Takashimizu I | 2001 | Ann Intern Med2001，13，4： | 1 |
| 4 | Improvement in atrophic gastritis and intetinal metaplasia in patients in whom Helicobacter pylori was eradicated 显示文摘 | Ohkusa T Fujiki K Takashimizu I | 2001 | Am Intern Med2001，134，： | 1 |
| 5 | Depositional facies and sedimentary successions of earthquake-induced tsunami deposits in upper Pleistocene incised valley fills, central Japan显示文摘 | Takashimizu Y Masuda F | 2000 | Sedimentary Geology2000，135，14： | 1 |
| 6 | Depositional facies and sedimentary successions of earthquake-induced tsunami deposits of Upper Pleistocene incised valley fills, central Japan 显示文摘 | Takashimizu Y Masuda F | 2000 | Sedimentary Geology2000，135，14： | 1 |
| 7 | Endoscopic and histological comparison of nonulcer dyspepsia with and without Helicobacter pylori infection cvaluatcd by the modified Sydney system显示文摘 | Ohkusa T Fujiki K Takashimizu I | | 0，，： | 1 |
| 8 | Improvement in atrophic gastritis and intestinal metaplasia in patients in whom Helicobacter pylori was eradicated显示文摘 | Ohkusa T Fujiki K Takashimizu I | | 0，，： | 1 |
| 9 | Clinical andpathological features of a prolonged type of acute intrahepaticcholestasis显示文摘 | Watanabe N Takashimizu S Kojima S | 2007 | Hepatol Res2007，37，8： | 1 |
| 10 | Disappearance of hyperplastic polyps in the stomach after eradication of Helicobacter pylori : a randomized, clinical trial显示文摘 | Ohkusa T Takashimizu I Fujiki K | 1998 | Ann Intern Med1998，129，9： | 1 |
| 11 | The outcome of patients withalcoholic liver cirrhosis 显示文摘 | Kojima S Ito H Takashimizu S | 2014 | Nihon Arukoru Yakubutsu IgakkaiZasshi2014，49，2： | 1 |
| 12 | Depositional facies and sedimentary successions of earthquake - induced tsunami deposits in Upper Pleistocene incised valley fills, central Japan 显示文摘 | Takashimizu Y Masuda F | 2000 | Sedimentary Geology2000，135，： | 1 |
| 13 | Efficacy of balloon -occluded retrograde transvenous obliteration (B -RTO) per- formed in a patient with primary biliary cirrhosis with severe re- current hepatic encephalopathy due to splenorenal shunt 显示文摘 | Takashimizu S Watanabe N Kojima S | 2007 | Tokai J Exp Clin Med2007，32，2： | 1 |
| 14 | Improvement in atrophic gastritis and intetinal metaplasia in patients in whom Helicobacter pylori was ersdicated显示文摘 | Ohkusa T Fujiki K Takashimizu I | 2001 | Ann Intern Med2001，134，： | 1 |
| 15 | Taurolithocholate impairs bile canalicular motility and canalicular bile secretion in isolated rat hepatocyte couplets显示文摘AIM: To investigate the effects of taurolithocholate (TLC)on the canalicular motility in isolated rat hepatocyte cou-plets (IRHC).METHODS: TLC was added to IRHC at concentrationsof 10 and 50 μmol/L, respectively. In each group, fi vetime-lapse movies containing 3 representative bile cana-liculi were taken under phase-contrast microscopy for12 h. The number of bile canalicular contractions andthe intervals between consecutive canalicular contrac-tions were calculated. Furthermore, the effects of TLC onIRHC were examined by transmission electron micros-copy.RESULTS: The bile canalicular contractions were spon-taneous and forceful in the controls. Active vesicularmovement was observed in the pericanalicular region.Immediately after the addition of TLC, the bile canaliculiwere deformed, and canalicular bile was incorporatedinto the vacuoles. The canaliculi were gradually dilated,and canalicular contractions were markedly inhibited byTLC. The vesicular movements became extremely slowin the pericanalicular region. The number of canalicularcontractions significantly decreased in the TLC-treatedgroups, as compared with that in the controls. The timeintervals were prolonged, as the TLC dosage increased,indicating that bile secretion into the canaliculi wasimpaired with TLC. Transmission electron microscopyrevealed the lamellar transformation of the canalicularmembranes in IRHC treated with TLC.CONCLUSION: TLC impairs both the bile canalicularcontractions and the canalicular bile secretion, possiblyby acting directly on the canalicular membranes in TLC-induced cholestasis. | Norihito Watanabe Tatehiro Kagawa Sei-ichiro Kojima Shinji Takashimizu Naruhiko Nagata Yasuhiro Nishizaki Tetsuya Mine | 2006 | World Journal of Gastroenterology2006，12，33： | 1 |
| 16 | An endothelin A receptor antagonist induces dilatation of sinusoidal endothelial fenestrae: implications for endothelin -1 in hepatic microcirculation 显示文摘 | Watanabe N Takashimizu S Nishizaki Y | 2007 | J Gastroenterol2007，42，9： | 1 |
| 17 | Clinical effects of infusing anti-epstein-barr virus (EBV)-specific cytotoxic T-lymphocytes into patients with severe chronic active EBV infection显示文摘 | Masao Hagihara Takahide Tsuchiya Osamu Hyodo Yoko Ueda Kei Tazume Aya Masui Ayako Kanemura Fumiaki Yoshiba Shinji Takashimizu Shohei Matzusaki Shunichi Kato Tomomitsu Hotta | 2003 | International Journal of Hematology2003，，1： | 1 |
| 18 | Depositional facies and sedimentary successions of earthquake-induced tsunami deposits in Upper Pleistocene incised valley fills, central Japan显示文摘 | Takashimizu Y Masuda F | 2000 | Sedimentary Geology2000，135，： | 1 |
| 19 | Improvement in atrophic gastritis and intetinal metaplasia in patients in whom Helicobacter pylori was eradicated显示文摘 | Ohkusa T Fujiki K Takashimizu I | 2001 | Ann Intern Med2001，134，： | 1 |
| 20 | Improve ment in atrophic gastritis and intetinal metaplasla in patients in whom Helicobacter pylori was eradicated 显示文摘 | ohkusa T Fujiki I Takashimizu I | 2001 | Ann intern Med2001，134，3： | 1 |
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