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| 1 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 2 | Postoperative long-term evaluation of interposition reconstruction compared with Roux-en-Y after total gastrectomy in gastric cancer: prospective randomized controlled trial显示文摘 | Sumiya Ishigami Shoji Natsugoe Shuichi Hokita Teruaki Aoki Hideyuki Kashiwagi Kosei Hirakawa Tetsuji Sawada Yoshitaka Yamamura Seiji Itoh Koichi Hirata Keiichiro Ohta Kenichi Mafune Yasushi Nakane Tatsuo Kanda Hiroshi Furukawa Iwao Sasaki Tetsuro Kubota M | 2011 | The American Journal of Surgery2011，，3： | 2 |
| 3 | Isolated small bowel transplantation outcomes and the impact of immunosuppressants: Experience of a single transplant center显示文摘AIM: To investigate patient and graft outcomes in isolated small bowel transplant(SBTx) recipients and immunosuppressant induction agent impact on outcomes.METHODS: A retrospective review of the perioperative data of patients who underwent SBTx transplant during an 8-year period was conducted. The intraoperative data were: patient demographics, etiology of short gut syndrome, hemodynamic parameters, coagulation profiles, intraoperative fluid and blood products transfused, and development of post-reperfusion. The postoperative data were: hospital/intensive care unit stays, duration of mechanical ventilation, postoperative incidence of acute kidney injury, and 1-year patient and graft outcomes. The effects of the three immunosuppressant induction agents(Zenapax, Thymoglobulin, Campath) on patient and graft outcomes were reviewed.RESULTS: During the 8-year period there were 77 patients; 1-year patient and graft survival were 95% and 86% respectively. Sixteen patients received Zenapax, 22 received Thymoglobulin, and 39 received Campath without effects on patient or graft survival(P = 0.90, P = 0.14, respectively). The use of different immune induction agents did not affect the incidence of rejection and infection during the first 90 postoperative days(P = 0.072, P = 0.29, respectively). The Zenapax group received more intraoperative fluid and blood products and were coagulopathic at the end of surgery. Zenapax and Thymoglobulin significantly increased serum creatinine at 48 h(P = 0.023) and 1 wk(P = 0.001) post-transplant, but none developed renal failure or required dialysis at the end of the first year.CONCLUSION: One-year patient and graft survival were 95% and 86%, respectively. The use of different immunosuppressant induction agents may affect the intraoperative course and short-term postoperative morbidities, but not 1-year patient and graft outcomes. | Ibtesam A Hilmi Raymond M Planinsic Ramona Nicolau-Raducu Daniela Damian Ali Al-Khafaji Tetsuro Sakai Kareem Abu-Elmagd | 2013 | World Journal of Transplantation2013，3，4： | 2 |
| 4 | Differential activation of the c-Jun N-terminal kinase/stress-activated protein kinase and p38mitogen-activated protein kinase signal transduction pathways in the mouse brain upon infection with neurovirulent influenza A virus显示文摘 | Isamu M Fumi G Tetsuro K | 2003 | J Gen Virol2003，84，9： | 1 |
| 5 | Cloning and functional expression of a chitinase cDNA from the comon cutworm, Spodoptera litura, using a recombinant baculovirus lacking the virus-encoded chitinase gene显示文摘 | Tetsuro S Jun K Masaharu M Yasuo C | | 0，，6： | 1 |
| 6 | Clinical usefulness of and problems with three - dimensional CT angiography for evaluation of arteriosclerotic stenosis of the carotid artery: comparison with conventional angiography, MRA, and uhrasound sonography显示文摘 | Tetsuro S Shigemi F Yoshihiro M | 1999 | Surg Neurol1999，51，3： | 1 |
| 7 | Pseudo-accoramodation with intraocular lenses im- planted in the bag显示文摘 | Altan-Yaycioglu R Gozum N Gucukoglu A Jorge L Tetsuro O Tatsuya M | 2002 | JRefrat Surg2002，18，3： | 1 |
| 8 | Mechanistic insight into the TiO2 photocatalytic reactions: design of new photocatalysts显示文摘 | TAHASHI T MAMORU F TETSURO M | 2007 | J Phys Chem C2007，111，： | 1 |
| 9 | Suppression of melanin synthesis by the phenolic constituents of sappanwood (Caesalpinia sappan)显示文摘 | Kaoru M Fumihide T Tetsuro K | 2013 | Planta Med2013，79，： | 1 |
| 10 | Preparation of mieroeapsulated enzymes for lowing the allergic activity of foods 显示文摘 | Tetsuro Y Kiyoshi M Yuichio K | 1997 | Journal of Agricultural and Food Chemistry1997，45，10： | 1 |
| 11 | Contagious yawning in chimpanzees显示文摘 | Anderson JR Masako MY Tetsuro M | 2004 | Proc Biol Sci2004，271，： | 1 |
| 12 | Japanese guidelines fr prevention of perioperative infections in urological field显示文摘 | Matsumoto T Kiyota H Matsukawa M Yasuda M Arakawa S Monden K Japanese Society of UTI Cooperative Study Group (Chairman Tetsuro Matsumoto) | 2007 | Int J Urol2007，14，： | 1 |
| 13 | Novel acrylic/polyester system for powder coatings 显示文摘 | Goro L Tetsuro A Kazuyoshi M | 1999 | Paint India1999，31，2： | 1 |
| 14 | Intramolecular Interaction of SUR2 Subtypes for Intracellular ADP-Induced Differential Control of KATP Channels显示文摘 | Kenji M Kengo K Tetsuro M | 2002 | Circ Res2002，90，： | 1 |
| 15 | Mechanistic insight into the TiO2 photocatalytic reactions: design of new photocatalysts显示文摘 | TAKASHI T MAMORU F TETSURO M | 2007 | J Phys Chem: C2007，111，14： | 1 |
| 16 | Adiponectin and atherosclerotic disease显示文摘 | Kazunori S Tetsuro M Hiroyuki D | 2004 | Clinica Chimica Acta2004，344，12： | 1 |
| 17 | Apparent accommodation and corneal wavefront aberration in pseudophakic eyes 显示文摘 | Tatsuya M Sumiyoshi T | 2002 | Investgative Ophthalmology&Vis Sci2002，43，： | 1 |
| 18 | Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana显示文摘 | TAISE S MIWA O TAKASHI S NAOTO M IKUKO H KENICHIOR S MASAYOSHI M AKIHO Y KENICHI T TETSURO M | 2004 | Plant Cell Physiology2004，45，6： | 1 |
| 19 | Accumulation of amyloid β protein in Tg2576 mice显示文摘 | Takeshi K Tetsuro M Etsuro M | 2003 | International Congress Series2003，，1252： | 1 |
| 20 | Intramolecular interaction of SUR2 subtypes for intracellular ADP-induced differential control of K+ATP channels显示文摘 | Kenji M Kengo K Tetsuro M el al | 2002 | Circ Res2002，90，5： | 1 |
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