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| 1 | In vitro derivation of functional insulin-producing cells from human embryonic stem cells显示文摘为人的胚胎的茎(ES ) 的自强和区别的能力细胞为对待类型 Idiabetes mellitus 为胰腺的贝它细胞的产生使他们成为潜在的来源。这里,我们报导一最新发展了并且有效方法,在aserum免费的系统执行了,区分进生产胰岛素的 cells.Activin A 的导致的人的 ES 房间它在起始的阶段被使用从人的 EScells 导致权威的内胚叶区别,是由权威的内胚叶标记 Sox17 和 Brachyury.Further 的表示检测了, all-trans retinoic 酸( RA )被用来支持胰腺的区别,由早胰腺的抄写因素 pdx1 和 hlxb9 的表示显示了。在成熟 inDMEM/F12 以后有 bFGF 和菸碱的没有浆液的媒介,区分的房间表示了小岛特定的标记象 C 肽,胰岛素,胰高血糖素和 glut2 那样。百分比 ofC-peptide-positive 房间超过了 15% 。由这些房间的胰岛素和 C 肽的分泌物在葡萄糖层次对应于变化。当移植了进肾的囊时, ofStreptozotocin (STZ ) 对待裸体老鼠,这些区分的人的 ES 房间熬过并且维持贝它房间标记基因的表示包括 C 肽, pdx1, glucokinase, nkx6.1, IAPP, pax6and Tcf1。百分之三十只移植裸体老鼠展出了 stableeuglycemia 的明显的恢复;并且改正的显型被支撑超过六个星期。我们的新方法为学习人的胰开发的机制提供一个有希望的试管内区别模特儿并且说明为类型 Idiabetes mellitus 的处理使用人的 ES 房间的潜力。 | Wei Jiang Yan Shi Dongxin Zhao Song Chen Jun Yong Jing Zhang Tingting Qing Xiaoning Sun Peng Zhang Mingxiao Ding Dongsheng Li Hongkui Deng | 2007 | Cell Research2007，17，4： | 38 |
| 2 | The chromosome-level quality genome provides insights into the evolution of the biosynthesis genes for aroma compounds of Osmanthus fragrans显示文摘Sweet osmanthus(Osmanthus fragrans)is a very popular ornamental tree species throughout Southeast Asia and USA particularly for its extremely fragrant aroma.We constructed a chromosome-level reference genome of O.fragrans to assist in studies of the evolution,genetic diversity,and molecular mechanism of aroma development.A total of over 118 Gb of polished reads was produced from HiSeq(45.1 Gb)and PacBio Sequel(73.35 Gb),giving 100×depth coverage for long reads.The combination of Illumina-short reads,PacBio-long reads,and Hi-C data produced the final chromosome quality genome of O.fragrans with a genome size of 727 Mb and a heterozygosity of 1.45%.The genome was annotated using de novo and homology comparison and further refined with transcriptome data.The genome of O.fragrans was predicted to have 45,542 genes,of which 95.68%were functionally annotated.Genome annotation found 49.35%as the repetitive sequences,with long terminal repeats(LTR)being the richest(28.94%).Genome evolution analysis indicated the evidence of whole-genome duplication 15 million years ago,which contributed to the current content of 45,242 genes.Metabolic analysis revealed that linalool,a monoterpene is the main aroma compound.Based on the genome and transcriptome,we further demonstrated the direct connection between terpene synthases(TPSs)and the rich aromatic molecules in O.fragrans.We identified three new flower-specific TPS genes,of which the expression coincided with the production of linalool.Our results suggest that the high number of TPS genes and the flower tissue-and stage-specific TPS genes expressions might drive the strong unique aroma production of O.fragrans. | Xiulian Yang Yuanzheng Yue Haiyan Li Wenjie Ding Gongwei Chen Tingting Shi Junhao Chen Min S.Park Fei Chen Lianggui Wang | 2018 | Horticulture Research2018，5，1： | 16 |
| 3 | GSA:Genome Sequence Archive显示文摘With the rapid development of sequencing technologies towards higher throughput and lower cost, sequence data are generated at an unprecedentedly explosive rate. To provide an efficient and easy-to-use platform for managing huge sequence data, here we present Genome Sequence Archive(GSA; http://gffzzdbc7b6aaae734bddh9kck5x9fn0oq69vn.ffgz.tsg.suse.edu.cn/gsa or http://gffzz4eaf941a184140e3h9kck5x9fn0oq69vn.ffgz.tsg.suse.edu.cn), a data repository for archiving raw sequence data. In compliance with data standards and structures of the International Nucleotide Sequence Database Collaboration(INSDC), GSA adopts four data objects(Bio Project, Bio Sample,Experiment, and Run) for data organization, accepts raw sequence reads produced by a variety of sequencing platforms, stores both sequence reads and metadata submitted from all over the world,and makes all these data publicly available to worldwide scientific communities. In the era of big data, GSA is not only an important complement to existing INSDC members by alleviating the increasing burdens of handling sequence data deluge, but also takes the significant responsibility for global big data archive and provides free unrestricted access to all publicly available data in support of research activities throughout the world. | Yanqing Wang Fuhai Song Junwei Zhu Sisi Zhang Yadong Yang Tingting Chen Bixia Tang Lili Dong Nan Ding Qian Zhang Zhouxian Bai Xunong Dong Huanxin Chen Mingyuan Sun Shuang Zhai Yubin Sun Lei Yu Li Lan Jingfa Xiao Xiangdong Fang Hongxing Lei Zhang Zhang Wenming Zhao | 2017 | Genomics, Proteomics & Bioinformatics2017，15，1： | 13 |
| 4 | Resveratrol attenuates bone cancer pain through regulating the expression levels of ASIC3 and activating cell autophagy显示文摘骨头癌症疼痛(BCP ) 是在有恶意的癌症的病人的最普通的疼痛之一。位于 BCP 下面的机制大部分是未知的。我们的以前的研究和增加的证据两个都显示出那个酸察觉到的离子隧道(ASIC3 ) 3 是处于在一些疼痛模型的病理学的疼痛状态的重要蛋白质。我们假设了 ASIC3 的表示变化可能是与 BCP 有关的因素之一。在这研究,我们通过 intrathecally 把老鼠乳腺癌房间(MRMT-1 ) 注入 Sprague-Dawley 雌老鼠的左胫骨建立了 BCP 模型,并且发现 BCP 老鼠在 L4-L6 背面的根中心显示出骨头破坏,增加的机械疼痛敏感和起来调整的 ASIC3 蛋白质表示层次。然后, resveratrol, intraperitoneally 在手术后的白天 21 上被注入 BCP 老鼠, dose-dependently 增加了 BCP 老鼠的爪退却阀值,颠倒了疼痛行为,并且有 BCP 老鼠上的 antinociceptive 效果。在 ASIC3-transfected SH-SY5Y 房间, ASIC3 蛋白质表示层次被 resveratrol 在一个剂量依赖者和时间依赖者举止调整。同时, resveratrol 也在调停 ASIC3 的疼痛老鼠模型有 antinociceptive 效果。而且, resveratrol 也在 ASIC3-transfected SH-SY5Y 房间提高了 AMPK, SIRT1,和 LC3-II 层次的 phosphorylation,显示 resveratrol 能激活在 ASIC3-transfected SH-SY5Y 房间的 AMPK-SIRT1-autophagy 信号小径。在 BCP 老鼠, SIRT1 和 LC3-II 也是下面调整的。这些调查结果在 BCP 状态期间作为一个治疗学的处理为 resveratrol 的使用提供新证据(?作者 2017。代表生物化学和房间生物学的研究所由牛津大学出版社出版了,为生物科学的上海研究所,中国科学院。版权所有。为权限,请发邮件:journals.permissions@oup.com。) | Haili Zhu Jieqiong Ding Ji Wu Tingting Liu Jing Liang Qiong Tang Ming Jiao | 2017 | Acta Biochimica et Biophysica Sinica2017，49，11： | 12 |
| 5 | A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants显示文摘Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable.Here,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation.We have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)analysis.Using tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic mutants.We produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic selection.Among these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction.This method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate. | Longzheng Chen Wei Li Lorenzo Katin-Grazzini Jing Ding Xianbin Gu Yanjun Li Tingting Gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy Frederick G.Gmitter Jr. Zhanao Deng Yunde Zhao Yi Li | 2018 | Horticulture Research2018，5，1： | 12 |
| 6 | Resorbable polymer electrospun nanofibers:History,shapes and application for tissue engineering显示文摘Resorbable polymer electrospun nanofiber-based materials/devices have high surface-to-volume ratio and often have a porous structure with excellent pore interconnectivity,which are suitable for growth and development of different types of cells.Due to the huge advantages of both resorbable polymers and electrospun nano fibers,re sorbable polymer electrospun nanofibers(RPENs)have been widely applied in the field of tissue engineering.In this paper,we will mainly introduce RPENs for tissue engineering.Firstly,the electrospinning technique and electrospun nanofiber architectures are briefly introduced.Secondly,the application of RPENs in the field of tissue engineering is mainly reviewed.Finally,the advantages and disadvantages of RPENs for tissue engineering are discussed.This review will provide a comprehensive guide to apply resorbable polymer electrospun nanofibers for tissue engineering. | Tingting Wu Mengzhen Ding Cuiping Shi Yiqun Qiao Panpan Wang Ruirui Qiao Xichang Wang Jian Zhong | 2020 | Chinese Chemical Letters2020，31，3： | 8 |
| 7 | Opposite effects of nitrogen fertilization and plastic film mulching on crop N and P stoichiometry in a temperate agroecosystem显示文摘Aims Crop nitrogen(N)and phosphorus(P)stoichiometry can influence food nutritive quality and many ecosystem processes.However,how and why N and P stoichiometry respond to long-term agricul-tural management practices(e.g.N fertilization and film mulching)are not clearly understood.Methods We collected maize tissues(leaf,stem,root and seed)and soil sam-ples from a temperate cropland under 30-year continuous N fer-tilization and plastic film mulching treatments,measured their C,N and P concentrations(the proportion(%)relative to the sample mass),and used structural equation models to uncover the re-sponding mechanisms for crop N and P contents(the total amount(g/m2)in crop biomass).Important Findings Long-term N fertilization increased N concentrations in all crop tissues but sharply decreased P concentrations in vegetative tis-sues(leaf,stem and root),thereby reducing their C/N ratio and increasing C/P and N/P ratios.The drop in P concentration in vegetative tissues was due to the dilution effect by biomass in-crement and the priority of P supply for seed production.In con-trast,film mulching decreased N concentration but increased P concentrations in most crop tissues,thereby increasing C/N ratio and reducing C/P and N/P ratios.Film mulching increased crop P content by increasing soil temperature and moisture;whereas,mulching showed little effect on crop N content,because a posi-tive effects of soil temperature may have canceled out a negative effect by soil moisture.This indicated a decoupling of P and N uptake by crops under film mulching.In conclusion,N fertiliza-tion and plastic film mulching showed opposite effects of on crop N and P stoichiometry. | Fan Ding Shuangyi Li Xiao-Tao Lü Feike A.Dijkstra Sean Schaeffer Tingting An Jiubo Pei Liangjie Sun Jingkuan Wang | 2019 | Journal of Plant Ecology2019，12，4： | 8 |
| 8 | Model tests on XCC-piled embankment under dynamic train load of high-speed railways显示文摘Piled embankments,which offer many advantages,are increasingly popular in construction of high-speed railways in China.Although the performance of piled embankment under static loading is well-known,the behavior under the dynamic train load of a high-speed railway is not yet understood.In light of this,a heavily instrumented piled embankment model was set up,and a model test was carried out,in which a servo-hydraulic actuator outputting M-shaped waves was adopted to simulate the process of a running train.Earth pressure,settlement,strain in the geogrid and pile and excess pore water pressure were measured.The results show that the soil arching height under the dynamic train load of a high-speed railway is shorter than under static loading.The growth trend for accumulated settlement slowed down after long-term vibration although there was still a tendency for it to increase.Accumulated geogrid strain has an increasing tendency after long-term vibration.The closer the embankment edge,the greater the geogrid strain over the subsoil.Strains in the pile were smaller under dynamic train loads,and their distribution was different from that under static loading.At the same elevation,excess pore water pressure under the track slab was greater than that under the embankment shoulder. | Niu Tingting Liu Hanlong Ding Xuanming Zheng Changjie | 2018 | Earthquake Engineering and Engineering Vibration2018，17，3： | 6 |
| 9 | Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death显示文摘细胞毒素的淋巴细胞是在有缺点的房间的有免疫力的反应和消除的组织的关键播放器。我们以前报导了生来的杀手(NK ) 房间进入目标肿瘤房间,导致在肿瘤房间以内的任何一个目标房间死亡或自我毁灭。然而,它留下了至于在成为主观以后的 NK 房间的命运逃犯并且 heterotypic cell-in-cell 过程最近是否与同型的 cell-in-cell 事件的不同,说出 entosis。这里,我们证明 NK 房间在肿瘤房间以内与 apoptosis 的最终的命运经历一个 cell-in-cell 过程并且表明成为主观过程要求肌动朊细胞骨架管理者, ezrin。设想 NK 房间怎么进入肿瘤房间,我们执行了 NK 房间成为主观的即时双颜色成像分析进肿瘤房间。令人惊讶地,大多数 NK 房间在他们的入口以后承诺规划房间死亡进肿瘤房间,它与在同型的 cell-in-cell 过程观察的 entosis 区别地不同。使内在化的 NK 房间的 apoptotic 房间死亡由 caspase 的激活是明显的 3 并且 DNA 破碎。而且,在成为主观以后的 NK 房间死亡被 caspase 禁止者稀释, Z-VAD-FMK,在肿瘤房间以内作为 NK 房间死亡的模式证实 apoptosis。决定为 NK 房间的入口必要的蛋白质因素进肿瘤房间,我们执行了基于 siRNA 的击倒的分析并且在 NK 房间成为主观发现了 ezrin 的一个关键角色。重要地, ezrin 的调停 PKA 的 phosphorylation 支持 NK 房间成为主观过程。我们的调查结果建议 ezrin 由管理 NK 房间成为主观进肿瘤房间的新奇规章的机制。 | Shan Wang Zhen Guo Peng Xia Tingting Liu Jufang Wang Shan Li Lihua Sun Jianxin Lu Qian Wen Mingqian Zhou Li Ma Xia Ding Xiaoning Wang Xuebiao Yao | 2009 | Cell Research2009，19，12： | 6 |
| 10 | Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens显示文摘A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution.A nonaxial,continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional(3-D)nanoscale shifts encoded.Then,by adopting a subvoxel-resolving procedure,the SLSM method models these low-resolution,cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the sample.This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times,with high acquisition speeds of gigavoxels per minute.By fast reconstruction of 3-D cultured cells,intact organs,and live embryos,SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue(>100 mm3)and observing single cell(∼1-μm resolution).It also eliminates the need of complicated mechanical stitching or modulated illumination,using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput,high-resolution 3-D microscopy,which could be tailored for a wide range of biomedical applications in pathology,histology,neuroscience,etc. | Peng Fei Jun Nie Juhyun Lee Yichen Ding Shuoran Li Hao Zhang Masaya Hagiwara Tingting Yu Tatiana Segura Chih-Ming Ho Dan Zhu Tzung KHsiaic | 2019 | Advanced Photonics2019，1，1： | 6 |
| 11 | ZBP1 mediates interferon-induced necroptosis显示文摘Interferons(IFNs)play an important role in immunomodulatory and antiviral functions.IFN-induced necroptosis has been reported in cells deficient in receptor-interacting protein kinase 1(RIPK1),Fas-associated protein with death domain(FADD),or caspase-8,but the mechanism is largely unknown.Here,we report that the DNA-dependent activator of IFN regulatory factors(ZBP1,also known as DAI)is required for both type Ⅰ(β)and type Ⅱ(γ)IFN-induced necroptosis.We show that L929 fibroblast cells became susceptible to IFN-induced necroptosis when RIPK1,FADD,or Caspase-8 was genetically deleted,confirming the antinecroptotic role of these proteins in IFN signaling.We found that the pronecroptotic signal from IFN stimulation depends on new protein synthesis and identified ZBP1,an IFN-stimulated gene(ISG)product,as the de novo synthesized protein that triggers necroptosis in IFN-stimulated cells.The N-terminal domain(ND)of ZBP1 is important for ZBP1–ZBP1 homointeraction,and its RHIM domain in the C-terminal region interacts with RIPK3 to initiate RIPK3-dependent necroptosis.The antinecroptotic function of RIPK1,FADD,and caspase-8 in IFN-treated cells is most likely executed by caspase-8-mediated cleavage of RIPK3,since the inhibitory effect on necroptosis was eliminated when the caspase-8 cleavage site in RIPK3 was mutated.ZBP1-mediated necroptosis in IFN-treated cells is likely physiologically relevant,as ZBP1 KO mice were significantly protected against acute systemic inflammatory response syndrome(SIRS)induced by TNF+IFN-γ. | Daowei Yang Yaoji Liang Shubo Zhao Yan Ding Qiuyu Zhuang Qilin Shi Tingting Ai Su-Qin Wu Jiahuai Han | 2020 | Cellular & Molecular Immunology2020，17，4： | 5 |
| 12 | A widely adaptable approach to generate integration-free iPSCs from non-invasively acquired human somatic cells显示文摘 | Zhichao Ding Lina Sui Ruotong Ren Yanjun Liu Xiuling Xu Lina Fu Ruijun Bai Tingting Yuan Ying Hao Weiqi Zhang Huize Pan Wensu Liu Han Yu Concepcion Rodriguez Esteban Xiaobing Yu Ze Yang Jian Li Xiaomin Wang Juan Carlos Izpisua Belmonte Guang-Hui Liu Fei Yi Jing Qu | 2015 | Protein & Cell2015，6，5： | 5 |
| 13 | Dynamic microwave-assisted extraction combined with liquid phase microextraction based on the solidification of a floating drop for the analysis of organochlorine pesticides in grains followed by GC显示文摘A convenient,cost-effective and fast method using dynamic microwave-assisted extraction and liquid phase microextraction based on the solidification of a floating drop was proposed to analyze organochlorine pesticides in grains including rice,maize and millet.Twelve samples can be processed simultaneously in the method.During the extraction process,10%acetonitrile-water solutions containing 110μL of n-hexadecane were used to extract organochlorine pesticides.Subsequently,1.0 g sodium chloride was placed in the extract,and then centrifuged and cooled.The n-hexadecane drops containing the analytes were solidifi ed and transferred for determination by gas chromatography-electron capture detector without any further filtration or cleaning process.Limits of detection for organochlorine pesticides were 0.97–1.01μg/kg and the RSDs were in the range of 2.6%–8.5%.The developed technology has succeeded in analyzing six real grains samples and the recoveries of the organochlorine pesticides were 72.2%–94.3%.Compared with the published extraction methods,the developed method was used to analyze organochlorine pesticides in grains,being more environmentally friendly,which is suitable for the daily determination of organochlorine pesticides. | Guijie Li Xu Zhang Tingting Liu Hongxiu Fan Hongcheng Liu Shangyu Li Dawei Wang Lan Ding | 2021 | Food Science and Human Wellness2021，10，3： | 4 |
| 14 | NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly显示文摘The impairment of mitochondrial bioenergetics,often coupled with exaggerated reactive oxygen species (ROS) production, is a fundamental disease mechanism in organs with a high demand for energy, including the heart. Building a more robust and safer cellular powerhouse holds the promise for protecting these organs in stressful conditions. Here, we demonstrate that NADH:ubiquinone oxidoreductase subunit AB1 (NDUFAB1), also known as mitochondrial acyl carrier protein, acts as a powerful cardio-protector by conferring greater capacity and efficiency of mitochondrial energy metabolism. In particular, NDUFAB1 not only serves as a complex I subunit, but also coordinates the assembly of respiratory complexes I, II, and III, and supercomplexes, through regulating iron-sulfur biosynthesis and complex I subunit stability. Cardiac-specific deletion of Ndufab1 in mice caused defective bioenergetics and elevated ROS levels, leading to progressive dilated cardiomyopathy and eventual heart failure and sudden death. Overexpression of Ndufab1 effectively enhanced mitochondrial bioenergetics while limiting ROS production and protected the heart against ischemia-reperfusion injury. Together, our findings identify that NDUFAB1 is a crucial regulator of mitochondrial energy and ROS metabolism through coordinating the assembly of respiratory complexes and supercomplexes, and thus provide a potential therapeutic target for the prevention and treatment of heart failure. | Tingting Hou Rufeng Zhang Chongshu Jian Wanqiu Ding Yanru Wang Shukuan Ling Qi Ma Xinli Hu Heping Cheng Xianhua Wang | 2019 | Cell Research2019，29，9： | 4 |
| 15 | The role of China's terrestrial carbon sequestration 2010-2060 in offsetting energy-related CO_(2) emissions显示文摘Energy consumption dominates annual CO_(2) emissions in China.It is essential to significantly reduce CO_(2) emissions from energy consumption to reach national carbon neutrality by 2060,while the role of terrestrial carbon sequestration in offsetting energy-related CO_(2) emissions cannot be underestimated.Natural climate solutions(NCS),including improvements in terrestrial carbon sequestration,represent readily deployable options to offset anthropogenic greenhouse gas emissions.However,the extent to which China’s terrestrial carbon sequestration in the future,especially when target-oriented managements(TOMs)are implemented,can help to mitigate energy-related CO_(2) emissions is far from certain.By synthesizing available findings and using several parameter-sparse empirical models that have been calibrated and/or fitted against contemporary measurements,we assessed China’s terrestrial carbon sequestration over 2010-2060 and its contribution to offsetting national energy-related CO_(2) emissions.We show that terrestrial C sequestration in China will increase from 0.375±0.056(mean±standard deviation)Pg C yr^(-1) in the 2010s to 0.458±0.100 Pg C yr^(-1)under RCP2.6 and 0.493±0.108 Pg C yr^(-1) under the RCP4.5 scenario in the 2050s,when TOMs are implemented.The majority of carbon sequestration comes from forest,accounting for 67.8-71.4%of the total amount.China’s terrestrial ecosystems can offset 12.2-15.0%and 13.4-17.8%of energy-related peak CO_(2) emissions in 2030 and 2060,respectively.The implementation of TOMs contributes 11.9%of the overall terrestrial carbon sequestration in the 2020s and 23.7%in the 2050s.The most likely strategy to maximize future NCS effectiveness is a full implementation of all applicable cost-effective NCS pathways in China.Our findings highlight the role of terrestrial carbon sequestration in offsetting energy-related CO_(2) emissions and put forward future needs in the context of carbon neutrality. | Yao Huang Wenjuan Sun Zhangcai Qin Wen Zhang Yongqiang Yu Tingting Li Qing Zhang Guocheng Wang Lingfei Yu Yijie Wang Fan Ding Ping Zhang | 2022 | National Science Review2022，9，8： | 4 |
| 16 | Fusobacterium nucleatum enhances the efficacy of PD-L1 blockade in colorectal cancer显示文摘Given that only a subset of patients with colorectal cancer(CRC)benefit from immune checkpoint therapy,efforts are ongoing to identify markers that predict immunotherapeutic response.Increasing evidence suggests that microbes influence the efficacy of cancer therapies.Fusobacterium nucleatum induces different immune responses in CRC with different microsatellite-instability(MSI)statuses.Here,we investigated the effect of F.nucleatum on anti-PD-L1 therapy in CRC.We found that high F.nucleatum levels correlate with improved therapeutic responses to PD-1 blockade in patients with CRC.Additionally,F.nucleatum enhanced the antitumor effects of PD-L1 blockade on CRC in mice and prolonged survival.Combining F.nucleatum supplementation with immunotherapy rescued the therapeutic effects of PD-L1 blockade.Furthermore,F.nucleatum induced PD-L1 expression by activating STING signaling and increased the accumulation of interferon-gamma(IFN-γ)^(+)CD8^(+)tumor-infiltrating lymphocytes(TILs)during treatment with PD-L1 blockade,thereby augmenting tumor sensitivity to PD-L1 blockade.Finally,patient-derived organoid models demonstrated that increased F.nucleatum levels correlated with an improved therapeutic response to PD-L1 blockade.These findings suggest that F.nucleatum may modulate immune checkpoint therapy for CRC. | Yaohui Gao Dexi Bi Ruting Xie Man Li Jing Guo Hu Liu Xianling Guo Juemin Fang Tingting Ding Huiyuan Zhu Yuan Cao Meichun Xing Jiayi Zheng Qing Xu Qian Xu Qing Wei Huanlong Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 3 |
| 17 | Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn’s disease显示文摘OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion. | Lu Yuan Zheng Handan Lü Tingting Ma Zhe Wu Huangan Xu Manwen Weng Zhijun Feng Hui Zhang Fang Ding Guanghong Wu Luyi Liu Huirong | 2019 | Journal of Traditional Chinese Medicine2019，39，3： | 3 |
| 18 | Hot compressive deformation behaviors of Mg–10Gd–3Y–0.5Zr alloy显示文摘In this paper, the hot compressive deformation characteristics of a Mg–10Gd–3Y–0.5Zr(GW103K) alloy have been investigated by isothermal compression test at the temperature range of 350–450°C and strain rate range of 0.0001–0.1s^(-1). True stress–strain relationships at various strain rates showed the typical strain hardening and softening stage which is indicative of dynamic recrystallization during deformation. The results showed that the peak stress was obviously dependent on temperature and strain rate. A constitutive equation to describe the deformation process was established based on the hyperbolic sine function. The stress exponent n and apparent activation energy Q were determined to be 3.018 and 203.947 k J/mol, respectively. Microstructure investigation showed that dislocation slipping was the dominant deformation mechanism during the hot deformation at all conditions. However, at the temperatures lower than 400 °C and strain rates higher than 0.01 s^(-1), twinning was observed to be activated, which indicated another deformation mechanism. Dynamic recrystallization and dynamic precipitation were found to occur simultaneously under such deformation condition. | Tingting Zheng Dejiang Li Xiaoqin Zeng Wenjiang Ding | 2016 | Progress in Natural Science:Materials International2016，26，1： | 2 |
| 19 | Identification of two novel mutations in the GALNT3 gene in a Chinese family with hyperphosphatemic familial tumoral calcinosis显示文摘Hyperphosphatemic familial tumoral calcinosis(HFTC) is a rare, autosomal recessive genetic disease.This disease is characterized by the progressive calcification of soft tissues leading to symptoms of pressure and hyperphosphatemia but normal concentrations of serum calcium with or without an elevation of1,25-dihydroxyvitamin D3 levels.HFTC is caused by loss-of-function mutations in the GALNT3, FGF23 or KL genes. Here, we identified two novel mutations in the GALNT3 gene in a Chinese family with HFTC.Identification of a novel genotype in HFTC provides clues for understanding the phenotype–genotype relationships in HFTC and may assist not only in the clinical diagnosis of HFTC but also in the interpretation of the genetic information used for prenatal diagnosis and genetic counseling. | Lihao Sun Lin Zhao Lianiun Du Peipei Zhang Minjia Zhang Min Li Tingting Liu Lei Ye Bei Tao Hongyan Zhao Jianmin Liu Xiaoyi Ding | 2016 | Bone Research2016，4，4： | 2 |
| 20 | On Quasi α- semicommutative rings 显示文摘 | ZHANG Peiyu WU Jun DING Tingting | 2013 | Journal of Hangzhou Normal Uni- versity (Natural Science Edition )2013，12，5： | 1 |
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