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| 1 | 新城疫病毒某水禽分离株经鸡体传代后由非致病型转变为速发型的研究显示文摘最近 ,一株无毒力的新城疫病毒 (NDV)在鸡体内繁殖时 ,变成了强毒株。但至今尚未能证实 ,经鸡体传代的野生水禽新城疫病毒是否也具有变成速发型毒株的能力。为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒 ,我们通过在鸡体内传播鹅源性无毒力株 ,经气囊接种连续传代 9次 ,随后再在鸡脑内传代 5次。结果显示 ,该病毒的毒力变得很强 ,致死率可达 1 0 0 %。通过致病性试验证实 ,其具有典型的速发型病毒特征 ;融合蛋白裂解位点的序列分析表明 ,原始的分离株含有无毒力型毒株共有的裂解序列 :E_R_Q_E_R/L,而通过鸡体反复传代后 ,该序列变为致病性毒株共有的序列 :K_R_Q_K_R/F。这些结果表明 ,野生水禽中自然存在的无毒力毒株 ,具有无毒力株相应的序列 ,但当其在鸡群中传播时 ,则具有变成高致病性病毒的能力。同时研究表明 ,鸡体提供了该病毒从非致病型向致病型转变的选择机制。 | 于圣青 丁铲 NORIKO KISHIDA HIROSHI ITO HIROSHI KIDA KOICHI OTSUKI YOSHIHIRO KAWAOKA TOSHIHIRO ITO | 2003 | 中国预防兽医学报2003，25，1： | 32 |
| 2 | Protective effect of estradiol on hepatocytic oxidative damage显示文摘AIM: To examine the protective effect of estradiol on the cultured hepatocytes under oxidative stress.METHODS: Hepatocytes of rat were isolated by using perfusion method, and oxidative stress wes induced by a serum-free medium and FeNTA. MDA level was determined with TBA method. Cell damage was assessed by LDH assay. Apoptosis of hepatocytes was assessed with cytoflowmetric analysis. Expression of Bcl-xl in cultured hepatocytes was detected by Western blot.The radicalscavenging activity of estradiol was valued by its ability to scavenge the stable free radical of DDPH.RESULTS: Oxidative stress increased LDH (from 168 ± 25 x10-6 IU. cell 1 to 780 ± 62 x 10-6 IU. cell-1 ) and MDA(from 0.28 ±0.07 x 10-6 nmol. cell-1 to 1. 35 ± 0.12 × 10-6 nmol. cell-1 ) levelsin cultured hepatocyte, and estradiol inhibited both LDH andMDA production in a dose dependent manner. In thepresence of estradiol t0-6 mol. L-1, 107 mol. L-1 and 10-8 mol.L-1 ,the LDH levels are 410 ± 53 × 10-6 IU. cell-1 ( P < 0.01 vsoxidative group), 530 ± 37 × 10-6 IU. cell-1 ( P < 0. 01 vsoxidative group), 687 ± 42 x 10-6 IU. cell-1 ( P < 0.05 vsoxidative group) respectively, and the MDA level are 0.71 ±0.12 x 10-6 nmol. cell-1 ( P < 0.01 vs oxidative group), 0.97 ± 0.11 × 10-6 nmol. cell-1 ( P < 0.01 vs oxidative group) and 1.27 ±0. 19 x 10-6 nmol. cell-1 respectively. Estradiol suppressedapoptosis of hepatocytes induced by oxidative stress,administration of estradiol (10-6 mol/ L)decreased theapoptotic rate of hepatocytes under oxidative stress from 18.6 ± 1.2% to 6.5 ± 2.5%, P < 0.01. Bcl-xl expression wasrelated to the degree of liver cell damage due to oxidativestress, and estradiol showed a protective action.CONCLUSION: Estmdiol protects hepatocytes from oxidativedamage by means of its antioxidant activity. | Ichiro Shimizu Toshihiro Omoya Susumu Ito | 2002 | World Journal of Gastroenterology2002，8，2： | 13 |
| 3 | Angiotensin receptor blocker neprilysin inhibitors显示文摘Heart failure(HF)is a clinical syndrome that results from a structural or functional cardiac disorder that reduces the ability of the ventricle of the heart to fill with,or eject,blood.It is a multifaceted clinical condition that affects up to 2%of the population in the developed world,and is linked to significant morbidity and mortality;it is therefore considered a major concern for public health.Regarding the mechanism of HF,three neurohumoral factors-the reninangiotensin-aldosterone system,the sympathetic nervous system,and natriuretic peptides—are related to the pathology of chronic HF(CHF),and the targets of treatment.Angiotensin receptor blocker and neprilysin inhibitor(angiotensinreceptor neprilysin inhibitor),namely sacubitril/valsartan(SAC/VAL),has been introduced as a treatment for CHF.SAC/VAL is an efficacious,safe,and costeffective therapy that improves quality of life and longevity in patients with HF with reduced ejection fraction(HFrEF),and reduces hospital admissions.An inhospital initiation strategy offers a potential new avenue to improve the clinical uptake of SAC/VAL.In the last five years,SAC/VAL has been established as a cornerstone component of comprehensive disease-modifying medical therapy in the management of chronic HFrEF.On the other hand,further work,with carefully designed and controlled preclinical studies,is necessary for understanding the molecular mechanisms,effects,and confirmation of issues such as long-term safety in both human and animal models. | Daisuke Usuda Toshihiro Higashikawa Yuta Hotchi Kenki Usami Shintaro Shimozawa Shungo Tokunaga Ippei Osugi Risa Katou Sakurako Ito Toshihiko Yoshizawa Suguru Asako Kentaro Mishima Akihiko Kondo Keiko Mizuno Hiroki Takami Takayuki Komatsu Jiro Oba Tomohisa Nomura Manabu Sugita | 2021 | World Journal of Cardiology2021，13，8： | 2 |
| 4 | Preparation and properties of Al2O3-Ni composite from NiAl2O4 spinel by in situ reaction sintering method显示文摘 | Toshihiro Isobe Keiji Daimon Kazunori Ito etc | 2006 | Ceramics International2006，，2430： | 1 |
| 5 | Cloning and sequencing of a nitrile hydratase gene from Pseudonocardia thermophila JCM3095显示文摘 | Toshifumi Yamaki Toshihiro Oikamw Kiyoshi Ito | 1997 | Journal of Fermentation and Bioengineering1997，83，： | 1 |
| 6 | Virulent influenza a vi- ruses induce apoptosis in chickens显示文摘 | Toshihiro Ito T Kobayashi Y Morita T | 2002 | Virus Research2002，84，12： | 1 |
| 7 | Cutting needle biopsy combined with immunohistochemical study of myeloperoxidase for the diagnosis of histiocytic necrotizing lymphadenitis显示文摘 | Hiroyuki Hanakawa Yorihisa Orita Yasuharu Sato Mai Takeuchi Kyotaro Ohno Noriko Iwaki Toshihiro Ito Kazunori Nishizaki Tadashi Yoshino | 2013 | Acta Oto-Laryngologica2013，，12： | 1 |
| 8 | In situ measurement of temperature distribution across a proton exchange membrane fuel cell显示文摘 | Lee S K Ito K Toshihiro O | 2009 | Electrochemical and Solid-State Letters2009，12，9： | 1 |
| 9 | Virulent influenza A viruses induce apoptosis in chickens显示文摘 | Toshihiro Ito T Kobayashi Y Morita T | 2012 | Virus Research2012，84，12： | 1 |
| 10 | New Aspects of Intra‐industry Trade in EU Countries显示文摘 | Tadashi Ito Toshihiro Okubo | 2012 | The World Economy2012，，9： | 1 |
| 11 | Biological Het- erogeneity, Including Systemic Replication in Mice, of HSN1 Influenza A Virus Isolates from Humans in Hang Kong 显示文摘 | Peng Gao Shinji Watanabe Toshihiro Ito | 1999 | American Society for Microbiology1999，73，4： | 1 |
| 12 | Generation of Velogenic Newcastle Disease Viruses from a Nonpathogenic Waterfowl Isolate by Passaging in Chickens显示文摘 | Yu Shengqing Noriko Kishida Hiroshi Ito Hiroshi Kida Koichi Otsuki Yoshihiro Kawaoka Toshihiro Ito | 2002 | Virology2002，，2： | 1 |
| 13 | Host range barrier of Influenza A viruses显示文摘 | Toshihiro Ito Yoshihiro Kawaoka | 2000 | Vet Microbiology2000，74，： | 1 |
| 14 | Trends of Second‐Line Eradication Therapy for Helicobacter pylori in Japan: A Multicenter Study in the Tokyo Metropolitan Area显示文摘 | Daisuke Asaoka Akihito Nagahara Takeshi Matsuhisa Shin‐ichi Takahashi Kengo Tokunaga Takashi Kawai Kohei Kawakami Hidekazu Suzuki Masayuki Suzuki Toshihiro Nishizawa Naoto Kurihara Masayoshi Ito Hitoshi Sasaki Fumio Omata Shigeaki Mizuno Akira Torii Toshi | 2013 | Helicobacter2013，，6： | 1 |
| 15 | Virulent influenza A Viruses induce apoptosis in chickens显示文摘 | Toshihiro Ito Yoshiyasu Kobayashi Jakehito Morita | 2002 | Virus Research2002，84，： | 1 |
| 16 | Virulent influenza A viruses induce apoptosis in chickens显示文摘 | Toshihiro Ito Yoshiyasu Kobayashi Takehito Morita | 2002 | Virus Res2002，84，： | 1 |
| 17 | Aprepitant in patients with advanced non-small-cell lung cancer receiving carboplatin-based chemotherapy显示文摘 | Yasuhiro Ito Masato Karayama Naoki Inui Shigeki Kuroishi Hideki Nakano Yutaro Nakamura Koshi Yokomura Mikio Toyoshima Toshihiro Shirai Masafumi Masuda Takashi Yamada Kazumasa Yasuda Hiroshi Hayakawa Takafumi Suda Kingo Chida | 2014 | Lung Cancer2014，，： | 1 |
| 18 | CCR6 as a mediator of immunity in the lung and gut显示文摘 | Toshihiro Ito William F. Carson Karen A. Cavassani Judith M. Connett Steven L. Kunkel | 2010 | Experimental Cell Research2010，，5： | 1 |
| 19 | Metastasis of the gastrointestinal tract: FDG-PET imaging显示文摘 | Kazumasa Hayasaka Takashi Nihashi Toshihiro Matsuura Tetsuya Yagi Kazumitsu Nakashima Yasuji Kawabata Kengo Ito Takashi Katoh Keita Sakata Atsushi Harada | 2007 | Annals of Nuclear Medicine2007，，6： | 1 |
| 20 | Sialic acid species as a determinant of the host range of influenza A viruses显示文摘 | Yasuo Suzuki Toshihiro Ito Takashi Suzuki | 2000 | J Virol2000，74，11： | 1 |
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