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| 1 | Seroepidemiological Investigation of Lyme Disease and Human Granulocytic Anaplasmosis among People Living in Forest Areas of Eight Provinces in China显示文摘Objective Lyme disease and Human granulocytic anaplasmosis are tick‐borne diseases caused by Borrelia burgdorferi and Anaplasma phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay (IFA). Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces (the exception being the Miyun area in Beijing). The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces. | HAO Qin GENG Zhen HOU Xue Xia TIAN Zhen YANG Xiu Jun JIANG Wei Jia SHI Yan ZHAN Zhi Fei LI Guo Hua YU De Shan WANG Hua Yong XU Jian Guo WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，3： | 13 |
| 2 | A Study of the Technique of Western Blot for Diagnosis of Lyme Disease caused by Borrelia afzelii in China显示文摘Objective To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure. Methods FP1, which is the representative strain of B. afzelii in China, was analyzed by SDS‐PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel‐Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accuracy of the WB as a diagnostic test was established by using the ROC curve and Youden index. Results Criteria for a positive diagnosis of Lyme disease were established as at least one band of P83/100, P58, P39, OspB, OspA, P30, P28, OspC, P17, and P14 in the IgG test and at least one band of P83/100, P58, P39, OspA, P30, P28, OspC, P17, and P41 in the IgM test. For IgG criteria, the sensitivity, specificity and Youden index were 69.8%, 98.3%, and 0.681, respectively; for IgM criteria, the sensitivity, specificity and Youden index were 47%, 94.2%, and 0.412, respectively. Conclusion Establishment of WB criteria for B. afzelii is important in validating the diagnostic assays for Lyme disease in China. | LIU Zhi Yun HAO Qin HOU Xue Xia JIANG Yi GENG Zhen WU Yi Mou WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，3： | 7 |
| 3 | Molecular Characterization and Drug-resistance of Mycobacterium tuberculosis Strains in Xuzhou, China显示文摘To understand the genetic diversity and drug resistance status of Mycobacterium tuberculosis(M tuberculosis)circulating in Xuzhou of China,the spacer-oligonucleotide typing(Spoligotyping)and multi-loci VNTRs(variable number tandem repeats)analysis(MLVA)were utilized for the genotyping of the isolates.Drug susceptibility test(DST) | PANG Hui TONG Jing LIU Hai Can DU Yang Guang ZHAO Xiu Qin JIANG Yi WU Xiao Cui YANG Jin Chuan WAN Kang Lin | 2014 | Biomedical and Environmental Sciences2014，27，12： | 7 |
| 4 | Dual-lens beam compression for optical coupling in superconducting nanowire single-photon detectors显示文摘The optical coupling of superconducting nanowire single-photon detectors(SNSPDs) has always been restricted to a single-mode fiber for a limited detection area. In this study, for enhancing photon coupling, a dual-lens system operating at 2.2 K was used to compress the beam size on the basis of the Gaussian beam theory and geometric approximation. A magnification of approximately 0.3 was obtained, and a focused spot with diameter of approximately 10 lm was measured from a multimode fiber. Assisted with the compressed beam, a system efficiency of 55 %(1550 nm) was achieved for a SNSPD with a detection area of 10 lm 9 10 lm and 62.5 lm multimode fiber coupling. At the same time, a high speed of106 MHz was measured with the proposed system. The realization of a highly compressed optical beam reduced the optical coupling requirement and helped maintain a high speed for the SNSPD. | Labao Zhang Chao Wan Min Gu Ruiying Xu Sen Zhang Lin Kang Jian Chen Peiheng Wu | 2015 | Science Bulletin2015，60，16： | 6 |
| 5 | Optimal timing for the oral administration of DaCheng-Qi decoction based on the pharmacokinetic and pharmacodynamic targeting of the pancreas in rats with acute pancreatitis显示文摘AIM To identify the optimal oral dosing time of Da-Cheng-Qi decoction(DCQD) in rats with acute pancreatitis(AP) based on the pharmacokinetic and pharmacodynamic parameters.METHODS First, 24 male Sprague-Dawley rats were divided into a sham-operated group [NG(a)] and three model groups [4 h G(a), 12 h G(a) and 24 h G(a)]. The NG(a) and model groups were administered DCQD(10 g/kg.BW) intragastrically at 4 h, 4 h, 12 h and 24 h, respectively, after AP models induced by 3% sodium taurocholate. Plasma samples were collected from the tails at 10 min, 20 min, 40 min, 1 h, 2 h, 4 h, 8 h, 12 h and 24 h after a single dosing with DCQD. Plasma and pancreatic tissue concentrations of the major components of DCQD were determined by high-performance liquid chromatography tandem mass spectroscopy. The pharmacokinetic parameters and serum amylase were detected and compared. Second, rats were divided into a sham-operated group [NG(b)] and three treatment groups [4 h G(b), 12 h G(b) and 24 h G(b)] with three corresponding control groups [MG(b)s]. Blood and pancreatic tissues were collected 24 h after a single dosing with DCQD. Serum amylase, inflammatory cytokines and pathological scores of pancreatic tissues were detected and compared.RESULTS The concentrations of emodin, naringin, honokiol, naringenin, aloe-emodin, chrysophanol and rheochrysidin in the 12 h G(a) group were higher than those in the 4 h G(a) group in the pancreatic tissues(P < 0.05). The area under the plasma concentration-time curve from time 0 to the time of the last measurable concentration values(AUC0→t) for rhein, chrysophanol, magnolol and naringin in the 12 h G(a) group were larger than those in the 4 h G(a) or 24 h G(a) groups. The 12 h G(a) group had a higher Cmax than the other two model groups. The IL-10 levels in the 12 h G(b) and 24 h G(b) groups were higher than in the MG(b)s(96.55 ± 7.84 vs 77.46 ± 7.42, 251.22 ± 16.15 vs 99.72 ± 4.7 respectively, P < 0.05), while in the 24 h G(b) group, the IL-10 level was higher than in the other two treatment groups(251.22 ± 16.15 vs 154.41 ± 12.09/96.55 ± 7.84, P < 0.05). The IL-6 levels displayed a decrease in the 4 h G(b) and 12 h G(b) groups compared to theMG(b)s(89.99 ± 4.61 vs 147.91 ± 4.36, 90.82 ± 5.34 vs 171.44 ± 13.43, P < 0.05). CONCLUSION Late-time dosing may have higher concentrations of the most major components of DCQD, with better pharmacokinetics and pharmacodynamics of antiinflammation than early-time dosing, which showed the late time to be the optimal dosing time of DCQD for AP. | Yu-Mei Zhang Lin Zhu Xian-Lin Zhao Huan Chen Hong-Xin Kang Jian-Lei Zhao Mei-Hua Wan Juan Li Lv Zhu Wen-Fu Tang | 2017 | World Journal of Gastroenterology2017，23，39： | 5 |
| 6 | A new Multilocus Sequence Analysis Scheme for Mycobacterium tuberculosis显示文摘Objective Tuberculosis remains one of the most serious infectious diseases in the world. In this study, a scheme of Mycobacterium tuberculosis (M. tuberculosis) multilocus sequence analysis (MLSA) was established for the phylogenetic and epidemiology analysis. Methods To establish the scheme of M. tuberculosis MLSA, the genome of H37Rv, CCDC5079 and CCDC5180 were compared, and some variable genes were chosen to be the MLSA typing scheme. 44 M. tuberculosis clinical isolates were typed by MLSA, IS6110-RFLP, and soligotyping, to evaluate the MLSA methods. Results After comparison of the genome, seven high discrimination gene loci (recX, rpsL, rmlC, rpmG1, mprA, gcvH, ideR) were chosen to be the MLSA typing scheme finally. 11 variable SNP sites of those seven genes were found among the 44 M. tuberculosis isolate strains and 11 sequence types (STs) were identified. Based on the Hunter-Gaston Index (HGI), MLSA typing was not as good for discrimination at the strain level as IS6110-RFLP, but the HGI was much better than that of spoligotyping. In addition, the MEGA analysis result of MLSA data was similar to spoligotyping/PGG lineage, showing a strong phylogenetic signal in the modern strains of M. tuberculosis. The MLSA data analysis by eBURST revealed that 4 sequence types (ST) came into a main cluster, showing the major clonal complexes in those 44 strains. Conclusion MLSA genotyping not only can be used for molecular typing, but also is an ideal method for the phylogenetic analysis for M. tuberculosis. | LU Bing DONG Hai Yan ZHAO Xiu Qin LIU Zhi Guang LIU Hai Can ZHANG Yuan Yuan JIANG Yi WAN Kang Lin | 2012 | Biomedical and Environmental Sciences2012，25，6： | 4 |
| 7 | Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674显示文摘Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine. | XIAO Tong Yang LIU Hai Can LI Xiao Qin HUANG Ming Xiang LI Gui Lian LI Na YAN Yu Han LUO Qiao WANG Xue Zhi LI Ma Chao WAN Kang Lin | 2019 | Biomedical and Environmental Sciences2019，32，6： | 3 |
| 8 | Novel Species Including Mycobacterium fukienense sp. Is Found from Tuberculosis Patients in Fujian Province, China, Using Phylogenetic Analysis of Mycobacterium chelonae/abscessus Complex显示文摘Objective To identify the novel species ‘Mycobacterium fukienense' sp. nov of Mycobacterium chelonae/abscessus complex from tuberculosis patients in Fujian Province, China. Methods Five of 27 clinical Mycobacterium isolates(Cls) were previously identified as M. chelonae/abscessus complex by sequencing the hsp65, rpoB, 16S-23S rRNA internal transcribed spacer region(its), recA and sodA house-keeping genes commonly used to describe the molecular characteristics of Mycobacterium. Clinical Mycobacterium isolates were classified according to the gene sequence using a clustering analysis program. Sequence similarity within clusters and diversity between clusters were analyzed. Results The 5 isolates were identified with distinct sequences exhibiting 99.8% homology in the hsp65 gene. However, a complete lack of homology was observed among the sequences of the rpoB, 16S-23S rRNA internal transcribed spacer region(its), sodA, and recA genes as compared with the M. abscessus. Furthermore, no match for rpoB, sodA, and recA genes was identified among the published sequences. Conclusion The novel species, Mycobacterium fukienense, is identified from tuberculosis patients in Fujian Province, China, which does not belong to any existing subspecies of M. chelonea/abscessus complex. | ZHANG Yuan Yuan LI Yan Bing HUANG Ming Xiang ZHAO Xiu Qin ZHANG Li Shui LIU Wen En WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，11： | 3 |
| 9 | Preliminary Study on Drug Susceptibility Profile and Resistance Mechanisms to Macrolides of Clinical Isolates of Non-tuberculous Mycobacteria from China显示文摘Objective Macrolide susceptibility and drug resistance mechanisms of clinical non-tuberculous mycobacteria(NTM) isolates were preliminarily investigated for more accurate diagnosis and treatment of the infection in China. Methods Four macrolides, including clarithromycin(CLAR), azithromycin(AZM), roxithromycin(ROX), and erythromycin(ERY), were used to test the drug susceptibility of 310 clinical NTM isolates from six provinces of China with the broth microdilution method. Two resistance mechanisms, 23 S r RNA and erm, were analyzed with nucleotide sequence analysis. Results Varied effectiveness of macrolides and species-specific resistance patterns were observed. Most Mycobacterium abscessus subsp. massiliense were susceptible and all M. fortuitum were highly resistant to macrolides. All the drugs, except for erythromycin, exhibited excellent activities against slow-growing mycobacteria, and drug resistance rates were below 22.2%. Only four highly resistant strains harbored 2,058/2,059 substitutions on rrl and none of other mutations were related to macrolide resistance. G2191 A and T2221 C on rrl were specific for the M. abscessus complex(MABC). Seven sites, G2140 A, G2210 C, C2217 G, T2238 C, T2322 C, T2404 C, and A2406 G, were specifically carried by M. avium and M. intracellulare. Three sites, A2192 G, T2358 G, and A2636 G, were observed only in M. fortuitum and one site G2152 A was specific for M. gordonae. The genes erm(39) and erm(41) were detected in M. fortuitum and M. abscessus and inducible resistance was observed in relevant sequevar. Conclusion The susceptibility profile of macrolides against NTM was demonstrated. The well-known macrolide resistance mechanisms, 23 S r RNA and erm, failed to account for all resistant NTM isolates, and further studies are warranted to investigate macrolide resistance mechanisms in various NTM species. | LI Fu LI Gui Lian PANG Hui LIU Hai Can XIAO Tong Yang LI Shuang Jun LUO Qiao JIANG Yi WANG Rui Bai WAN Kang Lin | 2018 | Biomedical and Environmental Sciences2018，31，4： | 3 |
| 10 | Strain heterogeneity,cooccurrence network,taxonomic composition and functional profile of the healthy ocular surface microbiome显示文摘Background:There is growing evidence indicating that the microbial communities that dwell on the human ocular surface are crucially important for ocular surface health and disease.Little is known about interspecies interactions,functional profiles,and strain heterogeneity across individuals in healthy ocular surface microbiomes.Methods:To comprehensively characterize the strain heterogeneity,cooccurrence network,taxonomic composition and functional profile of the healthy ocular surface microbiome,we performed shotgun metagenomics sequencing on ocular surface mucosal membrane swabs of 17 healthy volunteers.Results:The healthy ocular surface microbiome was classified into 12 phyla,70 genera,and 140 species.The number of species in each healthy ocular surface microbiome ranged from 6 to 47,indicating differences in microbial diversity among individuals.The species with high relative abundances and high positivity rates were Streptococcus pyogenes,Staphylococcus epidermidis,Propionibacterium acnes,Corynebacterium accolens,and Enhydrobacter aerosaccus.A correlation network analysis revealed a competitive interaction of Staphylococcus epidermidis with Streptococcus pyogenes in ocular surface microbial ecosystems.Staphylococcus epidermidis and Streptococcus pyogenes revealed phylogenetic diversity among different individuals.At the functional level,the pathways related to transcription were the most abundant.We also found that there were abundant lipid and amino acid metabolism pathways in the healthy ocular surface microbiome.Conclusion:This study explored the strain heterogeneity,cooccurrence network,taxonomic composition,and functional profile of the healthy ocular surface microbiome.These findings have important significance for the future development of probiotic-based eye therapeutic drugs. | Yutong Kang Shudan Lin Xueli Ma Yanlin Che Yiju Chen Tian Wan Die Zhang Jiao Shao Jie Xu Yi Xu Yongliang Lou Meiqin Zheng | 2021 | Eye and Vision2021，8，1： | 3 |
| 11 | Synthesis of N-methylene phosphonic chitosan(NMPCS)and its potential as gene carder显示文摘N-Methylene phosphoulc chitosan (NMPCS),an amphiphilic macromolecule with powerful chelating ability of Ca^(2+) ions,was synthesized and characterized.The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,^(13)C NMR,X-ray,agarose gel electrophorcsis retardation assay,atomic force microscopy (AFM) and circular dichroism (CD).The results suggest that at charge ratio 2:1 or above,DNA could be completely entrapped and spherical complexes with mean size of 80-210 nm were formed.Taking HeLa as host cell,luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan. | Dun Wan Zhu Jin Gen Bo Hai Ling Zhang Wen Guang Liu Xi Gang Leng Cun Xian Song Yu Ji Yin Li Ping Song Lan Xia Liu Lin Mei Xiu Lan Li Yang Zhang Kang De Yao | 2007 | Chinese Chemical Letters2007，18，11： | 3 |
| 12 | Optimization of Pulse-Field Gel Electrophoresis for Borrelia burgdorferi Subtyping显示文摘Objective To optimize the performance of Pulsed-Field Gel Electrophoresis (PFGE) for the comparison of inter-laboratory results and information exchange of Borrelia burgdorferi subtyping. Methods A panel of 34 strains of B. burgdorferi were used to optimize PFGE for subtyping. In order to optimize the electrophoretic parameters (EPs), all 34 strains of B. burgdorferi were analyzed using four EPs, yielding different Simpson diversity index (D) values and the epidemiological concordance was also evaluated. Results The EP of a switch time of 1 s to 25 s for 13 h and 1 s to 10 s for 6 h produced the highest D value and was declared to be optimal for MluI and SmaI PFGE of B. burgdorferi. MluI and SmaI were selected as the first and second restriction enzymes for PFGE subtyping of B. burgdorferi according to discrimination and consistency with epidemiological data. Conclusion PFGE can be used as a valuable test for routine genospecies identification of B. burgdorferi. | GENG Zhen HOU Xue Xia HAO Qin ZHOU Hai Jian WANG Feng WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，7： | 2 |
| 13 | Temperature dependence of niobium superconducting nanowire single-photon detectors in He-3 cryocooler显示文摘Performances of superconducting nanowire single-photon detectors(SNSPDs) based on low TCmaterials strongly depend on the operating temperatures. We have fabricated infrared-sensitive niobium SNSPDs based on doped niobium(Nb*) films and measured them in He-3cryocooler. The critical current approaches to the de-pairing current at 0.3 K. Therefore, with the decrease in temperatures, we have observed a monotonous increase of count rate at the wavelength of 1,521 nm and exponential decrease of dark count rate at all bias currents. The possible origin of dark counts for doped Nb devices is also discussed. | Tao Jia Chao Wan Limin Zhao Yu Zhou Qingyuan Zhao Min Gu Xiaoqing Jia Labao Zhang Biaobing Jin Jian Chen Lin Kang | 2014 | Chinese Science Bulletin2014，59，28： | 2 |
| 14 | A Retrospective Study of Culture-confirmed Mycobacterial Infection among Hospitalized HIV-infected Patients in Beijing, China显示文摘A retrospective analysis was performed in two major HIV/AIDS referral hospitals in Beijing to evaluate the prevalence of Mycobacterium tuberculosis(MTB) and non-tuberculous mycobacterial(NTM) infections in HIV-infected patients. A total of 627 patients' data were reviewed, and 102(16.3%) patients were diagnosed with culture-confirmed mycobacterial infection, including 84 with MTB, 16 with NTM, and 2 with both MTB and NTM. The most frequent clinical complication by mycobacterial infection was pulmonary infection(48/102, 47.1%). The overall rates of multidrug-resistant TB(MDR-TB) and extensively drug-resistant TB(XDR-TB) were 11.9% and 3.4%, respectively. This study underlines the urgent need to intensify screening for mycobacteria coinfection with HIV and to prevent the spread of drug-resistant TB among HIV-infected patients. | ZHAO Xiu Ying ZENG Zhao Ying HUA Wen Hao YU Yan Hua GUO Cai Ping ZHAO Xiu Qin DONG Hai Yan LIU Jie WAN Kang Lin | 2018 | Biomedical and Environmental Sciences2018，31，6： | 2 |
| 15 | Ischemic or toxic injury: A challenging diagnosis and treatment of drug-induced stenosis of the sigmoid colon显示文摘A 48-year-old woman was admitted with 15-mo history of abdominal pain, diarrhea and hematochezia, and 5-mo history of defecation difficulty. She had been successively admitted to nine hospitals, with an initial diagnosis of inflammatory bowel disease with stenotic sigmoid colon. Findings from computed tomography virtual colonoscopy, radiography with meglumine diatrizoate, endoscopic balloon dilatation, metallic stent implantation and later overall colonoscopy, coupled with the newfound knowledge of compound Qingdai pill-taking, led to a subsequent diagnosis of ischemic or toxic bowel disease with sigmoid colon stenosis. The patient was successfully treated by laparoscopic sigmoid colectomy, and postoperative pathological examination revealed ischemic or toxic injury of the sigmoid colon, providing a final diagnosis of druginduced sigmoid colon stenosis. This case highlights that adequate awareness of drug-induced colon stenosis has a decisive role in avoiding misdiagnosis and mistreatment. The diagnostic and therapeutic experiences learnt from this case suggest that endoscopic balloon expansion and colonic metallic stent implantation as bridge treatments were demonstrated as crucial for the differential diagnosis of benign colonic stenosis. Skillful surgical technique and appropriate perioperative management helped to ensure the safety of our patient in subsequent surgery after long-term use of glucocorticoids. | Zong-Ming Zhang Xiang-Chun Lin Li Ma An-Qin Jin Fang-Cai Lin Zhuo Liu Li-Min Liu Chong Zhang Na Zhang Li-Juan Huo Xue-Liang Jiang Feng Kang Hong-Jun Qin Qiu-Yang Li Hong-Wei Yu Hai Deng Ming-Wen Zhu Zi-Xu Liu Bai-Jiang Wan Hai-Yan Yang Jia-Hong Liao Xu Luo You-Wei Li Wen-Ping Wei Meng-Meng Song Yue Zhao Xue-Ying Shi Zhao-Hui Lu | 2017 | World Journal of Gastroenterology2017，23，21： | 2 |
| 16 | First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis显示文摘Objective In this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study. Methods Milk was preprocessed with 4% Na OH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction(PCR) and multi-locus sequence analysis with 16 S r RNA, sod A, hsp65, and ITS genes. The drug sensitivity of the isolate to 27 antibiotics was tested through alamar blue assay. Results Smooth, moist, pale yellow colonies appeared on the L-J medium within a week after inoculation. Based on the results of multiple loci PCR analysis, the isolate was preliminarily identified as non-tuberculous mycobacteria. The 16 S r RNA, Sod A, hsp65, and ITS gene sequences of the isolate exhibited 99%, 99%, 99%, and 100% similarities, respectively, with those of the published reference strains of Mycobacterium elephantis(M. elephantis). The drug sensitivity results showed that the strain is resistant to isoniazid, p-aminosalicylic acid, and trimesulf but is sensitive to ofloxacin, rifampicin, amikacin, capreomycin, moxifloxacin, kanamycin, levofloxacin, cycloserine, ethambutol, streptomycin, tobramycin, rifabutin, ciprofloxacin, linezolid, cefoxitin, clarithromycin, and minocycline. Conclusion To the best of our knowledge, this study is initially to report the isolation of M. elephantis from the milk of a cow with mastitis in China. | JI Ling Yun XU Dong Lei YIN Shu Peng LIU Hai Can LI Gui Lian JIANG Yi WEI Jian Hao ZENG Hao LOU Yong Liang LYU Jian Xin WAN Kang Lin | 2017 | Biomedical and Environmental Sciences2017，30，7： | 1 |
| 17 | Prognostic nutritional index predicts outcomes of malignant pleural mesothelioma显示文摘 | Zhou-Hong Yao Guang-Yan Tian Yun-Yan Wan Yan-Meng Kang Hai-Sheng Guo Qing-Hua Liu Dian-Jie Lin | 2013 | Journal of Cancer Research and Clinical Oncology2013，，12： | 1 |
| 18 | Rapid Detection of Rifampin-resistant Clinical Isolates of Mycobacterium tuberculosis by Reverse Dot Blot Hybridization显示文摘Objective A PCR-reverse dot blot hybridization(RDBH) assay was developed for rapid detection of rpo B gene mutations in ‘hot mutation region' of Mycobacterium tuberculosis(M. tuberculosis). Methods 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M. tuberculosis were designed to screen the most frequent wild-type and mutant genotypes for diagnosing RIF resistance. 300 M. tuberculosis clinical isolates were detected by RDBH, conventional drug-susceptibility testing(DST) and DNA sequencing to evaluate the RDBH assay. Results The sensitivity and specificity of the RDBH assay were 91.2%(165/181) and 98.3%(117/119), respectively, as compared to DST. When compared with DNA sequencing, the accuracy, positive predictive value(PPV) and negative predictive value(NPV) of the RDBH assay were 97.7%(293/300), 98.2%(164/167), and 97.0%(129/133), respectively. Furthermore, the results indicated that the most common mutations were in codons 531(48.6%), 526(25.4%), 516(8.8%), and 511(6.6%), and the combinative mutation rate was 15(8.3%). One and two strains of insertion and deletion were found among all strains, respectively. Conclusion Our findings demonstrate that the RDBH assay is a rapid, simple and sensitive method for diagnosing RIF-resistant tuberculosis. | GUO Qian YU Yan ZHU Yan Ling ZHAO Xiu Qin LIU Zhi Guang ZHANG Yuan Yuan LI Gui Lian WEI Jian Hao WU Yi Mou WAN Kang Lin | 2015 | Biomedical and Environmental Sciences2015，28，1： | 1 |
| 19 | Establishment of Multiple Locus Variable-number Tandem Repeat Analysis Assay for Genotyping of Borrelia burgdorferi sensu lato Detected in China显示文摘Objective Human Lyme Borreliosis(LB), which is caused by Borrelia burgdorferi sensu lato(B. burgdorferi), has been identified as a major arthropod-borne infectious disease in China. We aimed to develop a multiple locus variable-number tandem repeat(VNTR) analysis(MLVA) assay for the genotyping of Borrelia burgdorferi strains detected in China. Methods B. garinii PBi complete 904.246 kb chromosome and two plasmids(cp26 and lp54) were screened by using Tandem Repeats Finder program for getting potential VNTR loci, the potential VNTR loci were analyzed and identified with PCR and the VNTR loci data were analyzed and MLVA clustering tree were constrcted by using the categorical coefficient and the unweighted pair-group method with arithmetic means(UPGMA). Results We identified 5 new VNTR loci through analyzing 47 potential VNTR loci. We used the MLVA protocol to analyse 101 B. burgdorferi strains detected in China and finally identified 51 unique genotypes in 4 major clusters including B. burgdorferi sensu stricto(B.b.s.s), B. garinii, B. afzelii, and B. valaisiana, consistent with the current MLSA phylogeny studies. The allele numbers of VNTR-1, VNTR-2, VNTR-3, VNTR-4, and VNTR-5 were 7, 3, 9, 7, and 6. The Hunter-Gaston index(HGI) of five VNTR loci were 0.79, 0.22, 0.77, 0.71, and 0.67, respectively. The combined HGI of five VNTR loci was 0.96. Clustering of the strains of Xinjiang, Inner Mongolia and Heilongjiang was confirmed, and this situation was consistent with the close geographical distribution of those provinces. Conclusion The MLVA protocol esytablished in this study is easy and can show strains' phylogenetic relationships to distinguish the strains of Borrelia species. It is useful for further phylogenetic and epidemiological analyses of Borrelia strains. | ZHOU Xin HOU Xue Xia GENG Zhen ZHAO Rui WAN Kang Lin HAO Qin | 2014 | Biomedical and Environmental Sciences2014，27，9： | 1 |
| 20 | Electrochemical behavior of dopamine at a quercetin-SAM-modified gold electrode and analytical application显示文摘 | Kang Jin Wan Zhao Lin Lu Xiao Quan | 2005 | Original paper2005，9，： | 1 |
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