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| 1 | Overexpression of Dlx2 enhances osteogenic differentiation of BMSCs and MC3T3-E1 cells via direct upregulation of Osteocalcin and Alp显示文摘Genetic studies have revealed a critical role of Distal-homeobox (Dlx) genes in bone formation,and our previous study showed that Dlx2 overexpressing in neural crest cells leads to profound abnormalities of the craniofacial tissues.The aim of this study was to investigate the role and the underlying molecular mechanisms of Dlx2 in osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) and pre-osteoblast MC3T3-E1 cells.Initially,we observed upregulation of Dlx2 during the early osteogenesis in BMSCs and MC3T3-E1 cells.Moreover,Dlx2 overexpression enhanced alkaline phosphatase (ALP) activity and extracellular matrix mineralization in BMSCs and MC3T3-E1 cell line.In addition,micro-CT of implanted tissues in nude mice confirmed that Dlx2 overexpression in BMSCs promoted bone formation in vivo.Unexpectedly,Dlx2 overexpression had little impact on the expression level of the pivotal osteogenic transcription factors Runx2,Dlx5,Msx2,and Osterix,but led to upregulation of Alp and Osteocalcin (OCN),both of which play critical roles in promoting osteoblast maturation.Importantly,luciferase analysis showed that Dlx2 overexpression stimulated both OCN and Alp promoter activity.Through chromatin-immunoprecipitation assay and site-directed mutagenesis analysis,we provide molecular evidence that Dlx2 transactivates OCN and Alp expression by directly binding to the Dlx2-response cis-acting elements in the promoter of the two genes.Based on these findings,we demonstrate that Dlx2 overexpression enhances osteogenic differentiation in vitro and accelerates bone formation in vivo via direct upregulation of the OCN and Alp gene,suggesting that Dlx2 plays a crucial role in osteogenic differentiation and bone formation. | Jianfei Zhang Wenbin Zhang Jiewen Dai Xudong Wang Steve Guofang Shen | 2019 | International Journal of Oral Science2019，11，2： | 19 |
| 2 | Factors Associated with Thrombolysis Outcome in Ischemic Stroke Patients with Atrial Fibrillation显示文摘The outcome of early intravenous thrombolysis for ischemic stroke in patients with atrial fibrillation(AF)is worse than that without thrombosis. How to increase the efficacy of intravenous thrombolysis for AF-related ischemic stroke remains largely unknown. In this study, we investigated factors that influence the effect of intravenous thrombolysis in these patients. Our results showed that thrombolysis was independently associated with a favorable outcome(P \ 0.001) and did not influence the mortality of AF-related ischemic stroke, although it increased the risk of hemorrhage within 24 h after treatment. Risk factors for a poor outcome at admission were:heart failure(P = 0.045); high systolic pressure(P = 0.039); high blood glucose(P = 0.030); and a high National Institutes of Health Stroke Scale(NIHSS) score(P \ 0.001). Moreover, high systolic pressure at admission(P = 0.007), high blood glucose(P = 0.027), and a high NIHSS score(P \ 0.001) were independent risk factors for mortality at 3 months. Besides thrombolysis, a high NIHSS score(P = 0.006) and warfarin taken within 48 h before stroke onset(P = 0.032) were also independent risk factors for symptomatic hemorrhage within 24 h after treatment. Ischemic stroke patients with AF benefited from intravenous thrombolysis with recombinant tissue plasminogen activator within 4.5 h after stroke. | Qiuyun Zhao Xiaobo Li Wanli Dong Min Ye Yongjun Cao Meijuan Zhang Qiantao Cheng Junshan Zhou Guofang Chen Ming Yu Shanshan Hong Xiue Wei Bei Wang Guiyun Cui Peng Zhang Hong Ding Rongzhen Xu Yan Chen Yun Xu | 2016 | Neuroscience Bulletin2016，32，2： | 13 |
| 3 | Blocking autocrine VEGF signaling by sunitinib, an anti-cancer drug, promotes embryonic stem cell selfrenewal and somatic cell reprogramming显示文摘 | Guofang Chen Xinxiu Xu Lihong Zhang Yanbin Fu Min Wang Haifeng Gu Xin Xie | 2014 | Cell Research2014，24，9： | 8 |
| 4 | Present Status and Prospect of High-Frequency Electro-hydraulic Vibration Control Technology显示文摘Electro-hydraulic vibration equipment(EHVE)is widely used in vibration environment simulation tests,such as vehicles,weapons,ships,aerospace,nuclear industries and seismic waves replication,etc.,due to its large output power,displacement and thrust,as well as good workload adaptation and multi-controllable parameters.Based on the domestic and overseas development of high-frequency EHVE,dividing them into servo-valve controlled vibration equipment and rotary-valve controlled vibration equipment.The research status and progress of high-frequency electro-hydraulic vibration control technology(EHVCT)are discussed,from the perspective of vibration waveform control and vibration controller.The problems of current electro-hydraulic vibration system bandwidth and waveform distortion control,stability control,offset control and complex vibration waveform generation in high-frequency vibration conditions are pointed out.Combining the existing rotary-valve controlled high-frequency electro-hydraulic vibration method,a new twin-valve independently controlled high-frequency electro-hydraulic vibration method is proposed to break through the limitations of current electro-hydraulic vibration technology in terms of system frequency bandwidth and waveform distortion.The new method can realize independent adjustment and control of vibration waveform frequency,amplitude and offset under high-frequency vibration conditions,and provide a new idea for accurate simulation of high-frequency vibration waveform. | Yi Liu Tao Wang Guofang Gong Rujun Gao | 2019 | Chinese Journal of Mechanical Engineering2019，32，6： | 7 |
| 5 | A new calculation model of cutterhead torque and investigation of its influencing factors显示文摘Cutterhead torque is a crucial parameter for the design and operation of earth pressure balance(EPB) shield tunneling machine.However,the traditional calculation models of cutterhead torque are too rough or exist gross errors under variable geological conditions.In order to improve the precision of the calculation model of cutterhead torque,dynamic operation parameters are considered and a new model is proposed.Experiment is carried out on a 1.8 m shield machine test rig and the calculating result with the new model is compared with the experimental data to verify the validity of the new model.The relative error of the new model is as low as 4% at smooth stage and is reduced to 5% at the end of trembling stage.Based on the results of the new model and the test data obtained from the 1.8 m test rig and the construction site,the inner relationships between several operation parameters and cutterhead torque are investigated and some quantitative conclusions are obtained. | WANG LinTao GONG GuoFang SHI Hu YANG HuaYong | 2012 | Science China(Technological Sciences)2012，55，6： | 6 |
| 6 | Genome-and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus显示文摘Seed oil content(SOC)is a highly important and complex trait in oil crops.Here,we decipher the genetic basis of natural variation in SOC of Brassica napus by genome-and transcriptome-wide association studies using 505 inbred lines.We mapped reliable quantitative trait loci(QTLs)that control SOC in eight environments,evaluated the effect of each QTL on SOC,and analyzed selection in QTL regions during breeding.Six-hundred and ninety-two genes and four gene modules significantly associated with SOC were identified by analyzing population transcriptomes from seeds.A gene prioritization framework,POCKET(prioritizing the candidate genes by incorporating information on knowledge-based gene sets,effects of variants,genome-wide association studies,and transcriptome-wide association studies),was implemented to determine the causal genes in the QTL regions based on multi-omic datasets.A pair of homologous genes,BnPMT6s,in two QTLs were identified and experimentally demonstrated to negatively regulate SOC.This study provides rich genetic resources for improving SOC and valuable insights toward understanding the complex machinery that directs oil accumulation in the seeds of B.napus and other oil crops. | Shan Tang Hu Zhao Shaoping Lu Liangqian Yu Guofang Zhang Yuting Zhang Qing-Yong Yang Yongming Zhou Xuemin Wang Wei Ma Weibo Xie Liang Guo | 2021 | Molecular Plant2021，14，3： | 6 |
| 7 | Amniotic fluid-derived stem cells mixed with platelet rich plasma for restoration of rat alveolar bone defect显示文摘从羊膜的液体孤立的干细胞作为房间治疗和织物工程的一个有希望的候选人被显示出。然而, maxillofacial 骨头织物工程的地里的羊膜的导出液体的干细胞(AFSC ) 的试验性、现出症状之前的潜的应用仍然是有限的。在这研究,老鼠 AFSC 成功地在 vitro 被收获并且描绘。老鼠 AFSC 显示出典型 fibroblastoid 形态学,稳定的增长活动和多区别潜力。Flow-cytometry 分析为象 CD34 和 CD45 那样的造血的标记证明这些房间为 CD29, CD44,和 CD90 是积极的,当否定时。与血小板富人一起的 AFSCs-premixed 的再生表演在牙槽的骨头缺点的恢复的血浆(PRP ) 胶化进一步用一只修改老鼠被调查颚的牙槽的缺点模型。微型电脑断层摄影术和组织学的检查在操作与控制组作比较以后在 4 和 8 个星期与 PRP 胶化显示出 AFSCs-premixed 的一个优异再生能力。而且,植入的 AFSC 能在缺点地点幸存并且直接参予骨头织物新生。一起拿,这些结果为牙槽的缺点恢复显示了基于 AFSCs 的牙槽的骨头织物工程策略的可行性。 | Minjiao Wang Hongliang Li Jiawen Si Jiewen Dai Jun Shi Xudong Wang Lihe Guo Guofang Shen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 5 |
| 8 | The synergistic effect of 3D-printed microscale roughness surface and nanoscale feature on enhancing osteogenic differentiation and rapid osseointegration显示文摘Personalized precision therapy and rapid osseointegration are the main development directions of dental implants.3 D printing is a vital advanced manufacturing technology for personalized precision therapy.However,the osteogenesis of the 3 D printed Ti6 Al4 V implants is unsatisfactory.From the bionic perspective,the hierarchical micro/nano-topography can mimic the microenvironment of the multilevel structure of natural bone tissue and may endow the implant surface with superior bioactivity.In the present study,the hierarchical micro/nano-topography was successfully fabricated by construction the nanoscale feature on 3 D printed microscale roughness surface of 3 D-printed Ti6 Al4 V implants by alkali-heat treatment and hydrothermal treatment.Then the cell biological responses in vitro and osseointegration performance in vivo were systematically evaluated.The hierarchical micro/nano-topography evidently increased the roughness,improved the hydrophilicity and accelerated the hydroxyapatite deposition and mineralization,which significantly enhanced the adhesion,differentiation and extracellular matrix mineralization of bone marrow derived mesenchymal stromal cells(BMSCs).Most importantly,the hierarchical micro/nano-topography on 3 D-printed implants facilitated the new bone formation and rapid osseointegration in vivo.Our study suggested that 3 D-printed implant with micro/nano-topography may be a promising candidate to be applied in orthopedic field to meet the need of customized therapy and rapid osseointegration. | Hui Wang Jiaqiang Liu Chengtao Wang Steve Guofang Shen Xudong Wang Kaili Lin | 2021 | Journal of Materials Science & Technology2021，，4： | 5 |
| 9 | TCP7 functions redundantly with several Class Ⅰ TCPs and regulates endoreplication in Arabidopsis显示文摘TCP(TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR) proteins, a family of plantspecific transcription factors, play important roles in many developmental processes. However, genetic and functional redundancy among class I TCP limits the analysis of their biological roles. Here, we identified a dominantnegative mutant of Arabidopsis thaliana TCP7 named leaf curling-upward(lcu), which exhibits smaller leaf cells and shorter hypocotyls than the wild type, due to defective endoreplication. A septuple loss-of-function mutant of TCP7, TCP8, TCP14, TCP15, TCP21, TCP22, and TCP23 displayed similar developmental defects to those of lcu.Genome-wide RNA-sequencing showed that lcu and the septuple mutant share many misexpressed genes.Intriguingly, TCP7 directly targets the CYCLIN D1;1 (CYCD1;1) locus and activates its transcription. We determined that the C-terminus of TCP7 accounts for its transcriptional activation activity. Furthermore, the mutant protein LCU exhibited reduced transcriptional activation activity due to the introduction of an EAR-like repressive domain at its C-terminus. Together, these observations indicate that TCP7 plays important roles during leaf and hypocotyl development, redundantly, with at least six class I TCPs, and regulates the expression of CYCD1;1 to affect endoreplication in Arabidopsis. | Guofang Zhang Hongtao Zhao Chunguang Zhang Xiaoyun Li Yuanyuan Lyu Dongmei Qi Yanwei Cui Lin Hu Zhenjie Wang Zheng Liang Sujuan Cui | 2019 | Journal of Integrative Plant Biology2019，61，11： | 5 |
| 10 | Construction of ternary CuO/CuFe_(2)O_(4)/g-C_(3)N_(4)composite and its enhanced photocatalytic degradation of tetracycline hydrochloride with persulfate under simulated sunlight显示文摘In this study,a graphitic carbon nitride(g-C_(3)N_(4))based ternary catalyst Cu O/Cu Fe_(2)O_(4)/gC_(3)N_(4)(CCCN)is successfully prepared thorough calcination method.After confirming the structure and composition of CCCN,the as-synthesized composites are utilized to activate persulfate(PS)for the degradation of organic contaminant.While using tetracycline hydrochloride(TC)as pollutant surrogate,the effects of initial p H,PS and catalyst concentration on the degradation rate are systematically studied.Under the optimized reaction condition,CCCN/PS is able to give 99%degradation extent and 74%chemical oxygen demand removal in assistance of simulated solar light,both of which are apparently greater than that of either Cu O/Cu Fe_(2)O_(4)and pristine g-C_(3)N_(4).The great improvement in degradation can be assignable to the effective separation of photoinduced carriers thanks to the integration between Cu O/Cu Fe_(2)O_(4)and g-C_(3)N_(4),as well as the increased reaction sites given by the g-C_(3)N_(4)substrate.Moreover,the scavenging trials imply that the major oxidative matters involved in the decomposition are hydroxyl radicals(·OH),superoxide radicals(·O_(2)^(-))and photo-induced holes(h^(+)). | Lei Wang Xiaolei Ma Guofang Huang Rui Lian Jingwei Huang Houde She Qizhao Wang | 2022 | Journal of Environmental Sciences2022，34，2： | 5 |
| 11 | Differential expression of meat quality and intramuscular fat deposition related genes in Hanjiang black pigs显示文摘 | Guofang Wu Xin'e Shi Jiping Zhou Lei Wang Yuefeng Li Chengchuang Song Aoqi Xiang Peiyue Dong Shiduo Sun | 2014 | Acta Biochimica et Biophysica Sinica2014，46，12： | 3 |
| 12 | Construction of TiO_(2)-covalent organic framework Z-Scheme hybrid through coordination bond for photocatalytic CO_(2) conversion显示文摘In this work,a covalent organic framework(COF),which is constructed by the building blocks of[5,10,15,20-tetrakis(4-aminophenyl)porphinato]copper(Ⅱ)(CuTAPP)and p-benzaldehyde,is employed to integrate with TiO_(2) for the purpose of establishing a Z-scheme hybrid.Within the system,isonicotinic acid performs the role of a bridge that connects the two components through a coordination bond.Further photocatalytic application reveals the hybrid framework is able to catalyze CO_(2) conversion under simulated solar light,resulting in CO production rate of 50.5 μmol g^(-1)·h^(-1),about 9.9 and 24.5 times that of COF and pristine TiO_(2),respectively.The ameliorated catalytic performance owes much to the por-phyrin block acting as photosensitizer that augments the light absorbance,and the establishment of Z-scheme system between the inorganic and orga nic comp on ents that enhances the separati on of the carriers.In addition,the chemical bridge also ensures a steady usage and stable charge delivery in the catalysis.Our study sheds light on the development of versatile approaches to covalently in corporate COFs with inorga nic semic on ductors. | Lei Wang Guofang Huang Liang Zhang Rui Lian Jingwei Huang Houde She Chunli Liu Qizhao Wang | 2022 | Journal of Energy Chemistry2022，31，1： | 3 |
| 13 | Characteristics of SARS-CoV-2 Delta variant-infected individuals with intermittently positive retest viral RNA after discharge显示文摘SARS-CoV-2-infected patients will be discharged when viral ribonucleic acid(RNA)is consecutively negative at least twice over a 24-h interval.However,we have demonstrated that positive retest viral RNA,a particular clinical phenomenon initially observed in discharged SARS-CoV-2-infected patients in early 2020[1,2],is the occasional reoccurrence of Polymerase Chain Reaction(PCR)-detectable viral RNA but not reinfection[3]. | Lu Li Jingyan Tang Zhiwei Xie Qingxin Gan Guofang Tang Zhongwei Hu Huimin Zeng Jingrong Shi Jiaojiao Li Yan Li Changwen Ke Min Kang Dan Liang Huan Lu Yuwei Tong Xilong Deng Jinxin Liu Hongzhou Lu Fuxiang Wang Fengyu Hu Feng Li Nanshan Zhong Xiaoping Tang | 2022 | National Science Review2022，9，10： | 3 |
| 14 | Joint Source-Channel En/Decoding Based on a New Symbol-Level Joint Trellis显示文摘 | TU Guofang LIU Jianjun ZHANG Can WANG Qiang | 2011 | Chinese Journal of Electronics2011，20，1： | 2 |
| 15 | Impact of COVID-2019 on stroke services in China: survey from the Chinese Stroke Association显示文摘Background The COVID-19 pandemic and physical distancing guidelines have compelled stroke practices worldwide to reshape their delivery of care significantly.We aimed to illustrate how the stroke services were interrupted during the pandemic in China.Methods A 61-item questionnaire designed on Wenjuanxing Form was completed by doctors or nurses who were involved in treating patients with stroke from 1 February to 31 March 2020.Results A total of 415 respondents completed the online survey after informed consent was obtained.Of the respondents,37.8%,35.2%and 27.0%were from mild,moderate and severe epidemic areas,respectively.Overall,the proportion of severe impact(reduction>50%)on the admission of transient ischaemic stroke,acute ischaemic stroke(AIS)and intracerebral haemorrhage(ICH)was 45.0%,32.0%and 27.5%,respectively.Those numbers were 36.9%,27.9%and 22.3%;36.5%,22.1%and 22.6%;and 66.4%,47.5%and 41.1%in mild,moderate and severe epidemic areas,respectively(all p<0.0001).For AIS,thrombolysis was moderate(20%-50%reduction)or severely impacted(>50%),as reported by 54.4%of the respondents,while thrombectomy was 39.3%.These were 44.4%,26.3%;44.2%,39.4%;and 78.2%,56.5%,in mild,moderate and severe epidemic areas,respectively(all p<0.0001).For patients with acute ICH,39.8%reported the impact was severe or moderate for those eligible for surgery who had surgery.Those numbers were 27.4%,39.0%and 58.1%in mild,moderate and severe epidemic areas,respectively.For staff resources,about 20%(overall)to 55%(severe epidemic)of the respondents reported moderate or severe impact on the on-duty doctors and nurses.Conclusion We found a significant reduction of admission for all types of patients with stroke during the pandemic.Patients were less likely to receive appropriate care,for example,thrombolysis/thrombectomy,after being admitted to the hospital.Stroke service in severe COVID-19 epidemic areas,for example,Wuhan,was much more severely impacted compared with other regions in China. | Xia Wang Menglu Ouyang Cheryl Carcel Chen Chen Lingli Sun Jie Yang Yao Zhang Guofang Chen Shoujiang You Yongjun Cao Lu Ma Xin Hu Yi Sui Craig Anderson Lili Song Yongjun Wang David Wang | 2020 | Stroke & Vascular Neurology2020，5，4： | 2 |
| 16 | Small-signal modeling and parameter extraction method for a multigate GaAs pHEMT switch显示文摘This paper presents an accurate small-signal model for multi-gate GaAs pHEMTs in switching-mode.The extraction method for the proposed model is developed.A 2-gate switch structure is fabricated on a commercial 0.5μm AlGaAs/GaAs pHEMT technology to verify the proposed model.Excellent agreement has been obtained between the measured and simulated results over a wide frequency range. | Lin Luo Jun Liu Guofang Wang Yuxing Wu | 2020 | Journal of Semiconductors2020，41，3： | 2 |
| 17 | Formation of ettringite in Portland cement/calcium aluminate cement/cal- cium sulfate ternary system hydrates at lower temperatures 显示文摘 | Xu Linglin Wang Peiming Zhang Guofang | 2012 | Construction Building Mater2012，31，： | 1 |
| 18 | Modeling and analysis of thrust force for EPB shield tunneling machine显示文摘 | WANG Lintao GONG Guofang SHI Hu | 2012 | Automation in Construction2012，27，： | 1 |
| 19 | Modeling and analysis of thrust force for EPB shield tunneling machine显示文摘 | Lintao Wang Guofang Gong Hu Shi Huayong Yang | 2012 | Automation in Construction2012，，： | 1 |
| 20 | Optimization of Ultrasonic Extraction and Clean-up Protocol for the Determination of Polycyclic Aromatic Hydrocarbons in Marine Sediments by High-performance Liquid Chroma-tography Coupled with Fluorescence Detection显示文摘The procedures of ultrasonic extraction and clean-up were optimized for the determination of polycyclic aromatic hydrocarbons (PAHs) in marine sediments. Samples were ultrasonically extracted, and the extracts were purified with a miniaturized silica gel chromatographic column and analyzed with high performance liquid chromatography (HPLC) with a fluorescence detector. Ultrasonication with methanol-dichloromethane (2:1, v/v) mixture gave higher extraction efficiency than that with dichloromethane. Among the three elution solvents used in clean-up step, dichloromethane-hexane (2:3, v/v) mixture was the most satisfactory. Under the optimized conditions, the recoveries in the range of 54.82% to 94.70% with RSDs of 3.02% to 23.22% for a spiked blank, and in the range of 61.20% to 127.08% with RSDs of 7.61% to 26.93% for a spiked matrix, were obtained for the 15 PAHs studied, while the recoveries for a NIST standard reference SRM 1941b were in the range of 50.79% to 83.78% with RSDs of 5.24% to 21.38%. The detection limits were between 0.75 ng L-1 and 10.99 ng L-1for different PAHs. A sample from the Jiaozhou Bay area was examined to test the established methods. | PENG Xuewei YAN Guofang LI Xianguo GUO Xinyun ZHOU Xiao WANG Yan | 2012 | Journal of Ocean University of China2012，11，3： | 1 |
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