|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Genomic sequencing and analysis of the first imported Middle East Respiratory Syndrome Coronavirus(MERS CoV) in China显示文摘Dear Editors,Middle East Respiratory Syndrome(MERS)has spread to more than 23 countries since the first case of a MERS coronavirus(MERS Co V)infection was confirmed in Sep2012[1].Because MERS Co V has led to mortality as high as 40%,it has become one of the most important emerging pathogens threatening human health.Recently,the outbreak of MERS Co V,which resulted in 172 infections and | XIE Qian CAO YuJuan SU Juan WU XianBo WAN ChengSong KE ChangWen ZHAO Wei ZHANG Bao | 2015 | Science China(Life Sciences)2015，58，8： | 8 |
| 2 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 3 | A Case of New Variant COVID-19 First Emerging in South Africa Detected in Airplane Pilot—Guangdong Province,China,January 6,2021显示文摘On January 6,2021,Guangdong CDC successfully isolated the 501Y.V2 variant of coronavirus disease 2019(COVID-19)virus from a throat swab of a patient.The 501Y.V2 variant was first detected in South Africa,and the patient is a 55-year-old male airline pilot of South African nationality.He entered Guangzhou City,the capital of Guangdong Province,from Singapore on the evening of December 8,2020 and was subject to a COVID-19 test. | Fengjuan Chen Bosheng Li Peter Hao Yang Song Wenbo Xu Nankun Liu Chunliang Lei Changwen Ke | 2021 | China CDC weekly2021，3，2： | 5 |
| 4 | H_(3)PMo_(12)O_(40)-catalyzed coupling of diarylmethanols with epoxides/diols/aldehydes toward polyaryl-substituted aldehydes显示文摘A versatile heteropoly acid(H_(3)PMo_(12)O_(40))-catalyzed coupling of diarylmethanols with epoxides was established for the synthesis of polyaryl-substituted aldehydes.Furthermore,the catalytic system was also suitable for the reaction of diarylmethanols and diols/aldehydes.The application of such an earthabundant,readily accessible,and nontoxic catalyst provides a green approach for the construction of polyaryl-substituted aldehydes. | Guoping Yang Yufeng Liu Ke Li Wei Liu Bing Yu Changwen Hu | 2020 | Chinese Chemical Letters2020，31，12： | 4 |
| 5 | Stepwise prediction and statistical screening:B-cell epitopes on neuraminidase of human avian H_5N_1 virus显示文摘The B-cell epitopes of virus are associated with the antiviral drug and the vaccine screening. As the nucleotide sequences of neuraminidase (NA) of stain GD-01-06 were sequenced, we predicted the α-helix and β-fold structure and the indexes of the flexible regions of secondary structure of NA with methods of the Hydrophilicity plot by Kyte-Doolittle, the Surface probability plot by Emini and the Antigenic index by Jameson-Wolf, and then screened statistically the parameters to predict B-cell epitopes by the Hierarchical cluster and the Bivariate correlation and the quartiles with SPSS 13.0. The impact of variation of amino acids in NA on its epitopes was analyzed. The predictive results were evaluated by Wu’s Antigenic Index and SWISS-MODEL. We found that the most possible epitopes on NA were located within or nearby its N-terminal Nos. 120―137, 81―84, 408―415, 273―282, 429―432, 356―368, 46―55, 146―155, 341―350 and 198―209, which were the dominant regions of NA epitopes. Peptide 120―137 including the glycoprotein domain (NGT126―128) was first chosen as the B-cell epitopes on NA. NA in H5N1 strain isolated after 2003 lacked in No. 53 amino acid (I), resulting in an increase in the surface flexible region of NA in GD-01-06 and an enlargement to their epitope regions (VEP46―48→ VEPISNTNFL46―55). Conclusively, prediction of the B-cell epitopes on the NA based on multiple parameters is useful for researches on the molecular immunology and drug screening and immuno-prophylaxis. A deletion of No. 53 amino acid (I) in NA in strain GD-01-06 might increase its antigenicity. | HUANG Ping YU ShouYi KE ChangWen | 2008 | Chinese Science Bulletin2008，53，23： | 4 |
| 6 | Antibody neutralization to SARS-CoV-2 and variants after 1 year in Wuhan,China显示文摘Most COVID-19 convalescents can build effective anti-SARS-CoV-2 humoral immunity,but it remains unclear how long it can maintain and how efficiently it can prevent the reinfection of the emerging SARS-CoV-2 variants.Here,we tested the sera from 248 COVID-19 convalescents around 1 year post-infection in Wuhan,the earliest known epicenter.SARS-CoV-2 immunoglobulin G(IgG)was well maintained in most patients and potently neutralizes the infection of the original strain and the B.1.1.7 variant.However,varying degrees of immune escape was observed on the other tested variants in a patient-specific manner,with individuals showing remarkably broad neutralization potency.The immune escape can be largely attributed to several critical spike mutations.These results suggest that SARS-CoV-2 can elicit long-lasting immunity but this is escaped by the emerging variants. | Qianyun Liu Qing Xiong Fanghua Mei Chengbao Ma Zhen Zhang Bing Hu Junqiang Xu Yongzhong Jiang Faxian Zhan Suhua Zhou Li Tao Xianying Chen Ming Guo Xin Wang Yaohui Fang Shu Shen Yingle Liu Fang Liu Li Zhou Ke Xu Changwen Ke Fei Deng Kun Cai Huan Yan Yu Chen Ke Lan | 2022 | The Innovation2022，3，1： | 3 |
| 7 | Characteristics of SARS-CoV-2 Delta variant-infected individuals with intermittently positive retest viral RNA after discharge显示文摘SARS-CoV-2-infected patients will be discharged when viral ribonucleic acid(RNA)is consecutively negative at least twice over a 24-h interval.However,we have demonstrated that positive retest viral RNA,a particular clinical phenomenon initially observed in discharged SARS-CoV-2-infected patients in early 2020[1,2],is the occasional reoccurrence of Polymerase Chain Reaction(PCR)-detectable viral RNA but not reinfection[3]. | Lu Li Jingyan Tang Zhiwei Xie Qingxin Gan Guofang Tang Zhongwei Hu Huimin Zeng Jingrong Shi Jiaojiao Li Yan Li Changwen Ke Min Kang Dan Liang Huan Lu Yuwei Tong Xilong Deng Jinxin Liu Hongzhou Lu Fuxiang Wang Fengyu Hu Feng Li Nanshan Zhong Xiaoping Tang | 2022 | National Science Review2022，9，10： | 3 |
| 8 | A H9N2 Human Case and Surveillance of Avian Influenza Viruses in Live Poultry Markets-Huizhou City,Guangdong Province,China,2021显示文摘Summary What is already known about this topic?An increasing number of human infected avian influenza A(H9N2)cases have been reported.In 2021,11 human infections with influenza A virus subtype H9N2(A/H9N2)have been reported in China.What is added by this report?A new case of H9N2 that occurred in April 2021 in Huizhou City,Guangdong Province,China,was reported in this study.Epidemiological and laboratory information of the case and routine influenza surveillance data of avian influenza A were presented in this report.What are the implications for public health practice?The emergence of a human infected with Avian Influenza Virus H9N2 demonstrates that there is an urgent need to strengthen the surveillance of influenza-like illness and live poultry market. | Guanting Zhang Liqin Xu Jian Zhang Qiaoyun Fang Jianjun Zeng Yan Liu Changwen Ke | 2022 | China CDC weekly2022，4，1： | 2 |
| 9 | Significantly reduced abilities to cross-neutralize SARS-CoV-2 variants by sera from convalescent COVID-19 patients infected by Delta or early strains显示文摘The worldwide pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in more than 4.5 million deaths.Although coronaviruses have a proofreading mechanism to maintain the stability of their long genomic RNAs,mutations emerge continuously,and new variants conferring advantages rapidly become the dominant lineages[1].Strategies to fight the COVID-19 pandemic using either vaccines or nonpharmaceutical interventions have specifically been threatened by the emergence of SARS-CoV-2 variants of concern(VOCs)[2]. | Ting Pan Zhongwei Hu Fengyu Hu Yiwen Zhang Bingfeng Liu Changwen Ke Qiumin She Xin He Xiaoping Tang Hui Zhang | 2021 | Cellular & Molecular Immunology2021，18，11： | 1 |
| 10 | Phylogenetic and molecular characterization of coxsackievirus A24variant isolates from a 2010acute hemorrhagic conjunctivitis outbreak in Guangdong,China显示文摘 | Wu De Zheng Huanying Li Hui Monagin Corina Guo Xue Liu Leng Zeng Hanri Fang Ling Mo Yanling Zhou Huiqiong Zhang Huan Kou Jing Long Caiyun Hiromu Yoshida and Ke Changwen | 2012 | Virol J2012，9，41： | 1 |
| 11 | Genetic diversity, linkage disequilibrium, and association mapping analyses of peach ( Prunus persica ) landraces in China显示文摘 | Ke Cao Lirong Wang Gengrui Zhu Weichao Fang Changwen Chen Jing Luo | 2012 | Tree Genetics & Genomes2012，，5： | 1 |
| 12 | Genomic analysis provides insights into the westward expansion of domesticated peaches in China显示文摘Peach, an economically important model plant of the Rosaceae family, has been domesticated and cultivated in China for approximately5 000 years. The Hexi Corridor, an important corridor connecting east-central China and the Tarim Basin, is the starting point of the Silk Road that links China and the Eurasian region. As a globally distributed fruit tree, the spread of peach was accomplished through historical trade routes in the Hexi Corridor and the Tarim Basin. However, knowledge of peach genetic diversity in these regions remains limited. In this study,we examined the relationships and the spread history of domesticated peaches through sequencing and genomic analysis of 161 peach accessions collected from Northwest China, including 43 from the Hexi Corridor and 104 from the Tarim Basin. The results indicate that peach landraces in the Hexi Corridor and the Tarim Basin are derivatives of peaches from the east and south of China (ESC). Notably, the genetic diversity of accessions from both the Hexi Corridor and the Tarim Basin was lower than that of ESC accessions. Reduction of diversity (ROD) and linkage disequilibrium (LD) analyses detected a genetic bottleneck in peaches from these regions. Additionally, these peaches have undergone varying degrees of selection from natural environment. Moreover, genes responsive to biotic and abiotic stresses were under selection, which could be the result of the climate change of Northwest China after the Last Glacial Maximum (LGM). Our findings provide a better understanding of the genetic basis of peach migration in Northwest China. Furthermore, this study expands the available genomic data for peaches and provides critical information for future peach breeding programs. | Wenqing Li Yong Li Xinwei Wang Guoqing Zhao Gengrui Zhu Ke Cao Weichao Fang Jinlong Wu Kai Ma Changwen Chen Lirong Wang | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 13 | Self-assembly of a new 3D platelike ternary-oxo-cluster:An efficient catalyst for the synthesis of pyrazoles显示文摘A new metal-oxo-clusters-based inorganic framework[NaCo_(2)Mo_(2)O_(7)(OH)_(3)]_(n)(NaCoMo),named as 3D platelike ternary-oxo-cluster,has been hydrothermally synthesized and characterized by single-crystal X-ray diffraction structure analysis,FT-IR spectroscopy,powder X-ray diffraction(PXRD),scanning electron microscope(SEM),energy-dispersive X-ray spectroscopy(EDS)analyses,X-ray photoelectron spectroscopy(XPS)and thermogravimetric analysis(TGA).Structure analysis reveals that there are no classical building units in NaCoMo,and the asymmetric units of NaCoMo are directly extended into a new platelike 3D structure.Density functional theory calculations(DFT)indicates that the crystal formation process is exothermic and the structure is extremely stable.In addition,the compound presents excellent catalytic activity in the condensation and cyclization reaction of sulfonyl hydrazides and 1,3-diketones to synthesize pyrazoles,and the yield of the desired product is up to 99%.The successful synthesis of NaCoMo represents the discovery of a new kind of non-classical polyoxometalates. | Guoping Yang Yufeng Liu Xiaoling Lin Bangming Ming Ke Li Changwen Hu | 2022 | Chinese Chemical Letters2022，33，1： | 0 |
| 14 | Infection of wild-type mice by SARS-CoV-2 B.1.351 variant indicates a possible novel cross-species transmission route显示文摘COVID-19 is identified as a zoonotic disease caused by SARS-CoV-2,which also can cross・transmit to many animals but not mice.Genetic modifications of SARS-CoV-2 or mice enable the mice susceptible to viral infection.Although neither is the natural situation,they are currently utilized to establish mouse infection models.Here we report a direct contact transmission of SARS-CoV-2 variant B.1.351 in wild-type mice.The SARS-CoV-2(B.1.351)re plicated efficiently and induced significant pathological changes in lungs and tracheas,accompanied by elevated proinflammatory cytokines in the lungs and sera.Mechanistically,the receptor-binding domain(RBD)of SARS-CoV-2(B.1.351)spike protein turned to a high binding affinity to mouse angiotensin-converting enzyme 2(mACE2),allowing the mice highly susceptible to SARS-CoV-2(B.1.351)infection.Our work suggests that SARS-CoV-2(B.1.351)expands the host range and therefore increases its transmission route without adapted mutation.As the wild house mice live with human populations quite closely,this possible transmission route could be potentially risky.In addition,because SARS-CoV-2(B.1.351)is one of the major epidemic strains and the mACE2 in laboratory-used mice is naturally expressed and regulated,the SARS-CoV-2(B.1.351)/mice could be a much convenient animal model system to study COVID-19 pathogenesis and evaluate antiviral inhibitors and vaccines. | Ting Pan Ran Chen Xin He Yaochang Yuan Xiaohui Deng Rong Li Haiping Yan Shumei Yan Jun Liu Yiwen Zhang Xiantao Zhang Fei Yu Mo Zhou Changwen Ke Xiancai Ma Hui Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 15 | Peculiarity of transcriptional and H3K27me3 dynamics during peach bud dormancy显示文摘Bud dormancy facilitates the survival of meristems under harsh environmental conditions.To elucidate how molecular responses to chilling accumulation controlling dormancy in peach buds,chromatin immunoprecipitation sequencing to identify the H3K27me3 modifications and RNA sequencing of two peach cultivars with pronounced differences in chilling requirement were carried out,the results showed that genes associated with abscisic acid and gibberellic acid signal pathways play key roles in dormancy regulation.The results demonstrated that peach flower bud differentiation occurred continuously in both cultivars during chilling accumulation,which was correlated with the transcript abundance of key genes involved in phytohormone metabolism and flower bud development under adverse conditions.The more increased strength in high chillingrequirement cultivar along with the chilling accumulation at the genome-wide level.The function of the dormancy-associated MADS-box gene PpDAM6 was identified,which is involved in leaf bud break in peach and flower development in transgenic Nicotiana tabacum(NC89).In addition,PpDAM6 was positively regulated by PpCBF,and the genes of putative dormancy-related and associated with metabolic pathways were proposed.Taken together,these results constituted a theoretical basis for elucidating the regulation of peach bud dormancy transition. | Yalin Zhao Yong Li Ke Cao Weichao Fang Changwen Chen Xinwei Wang Jinlong Wu Wenwu Guo Lirong Wang | 2024 | Horticultural Plant Journal2024，10，1： | 0 |
| 16 | Long-term asymptomatic SARS-CoV-2 infection associated with deficiency on multiple immune cells显示文摘The immune responses and the function of immune cells among asymptomatic severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)infection cases,especially in immuno‐compromised individuals,remain largely unknown.Here we present a case of asymptomatic SARS‐CoV‐2 infection that lasted for at least 67 days.The patient has administrated Thymalfasin as 1.6 mg per dose every other day from Day 45 to 70,plus 200 mg per dose Arbidol antiviral therapy three doses per day from Day 48 to 57.Throughout the infection,no anti‐SARS‐CoV‐2 specific IgM or IgG antibodies were detected.Instead,the patient showed either a low percentage or an absolute number of non‐classical monocytes,dendritic cells(DCs),CD4^(+)T cells,and regulatory T cells(Tregs),which may account for the clinical feature and absence of antibody response.This case may shed new light on the outbreak management related to control/prevention,treatment,and vaccination of SARS‐CoV‐2 and other virus infections in immunocompromised individuals. | Gang He Xia Chuai Dan Liang Chunyu Chen Changzheng Hu Changwen Ke Bixia Ke Peilin Zhen Huajun Zhang | 2022 | Biosafety and Health2022，4，3： | 0 |
| 17 | A comparative study on environmental surveillance of enterovirus:Using a two-phase separation method and a filtration method with a mixed cellulose ester(MCE)membrane显示文摘This study aimed to compare the sensitivity of two-phase separation and the filtration method using a mixed cellulose ester(MCE)membrane to detect enteroviruses in sewage samples.From December 2015 to July 2016,four domestic sewage samples(1 L/sample)were collected monthly from the Guangzhou Liede Sewage Treatment Plant,and each sewage sample was divided into two aliquots(500 mL).The sewage sample was concentrated using the two-phase separation and the filtration method using an MCE membrane,and the treated solutions were inoculated into cells for enterovirus isolation.Polymerase chain reaction amplification,VP1 sequencing,and enterovirus molecular typing were performed on the positive isolates.The detection rates of poliovirus(PV)and non-polio enterovirus(NPEV)obtained using the filtration method using an MCE membrane were higher than those using the two-phase separation method.McNemar's test showed that the detection rates of PV,NPEV,type 1 Sabin-like(SL1),type 2 Sabin-like(SL2),and type 3 Sabin-like(SL3)strain were not statistically significant(P>0.05).In Guangdong Province,China,the detection rates for PV and NPEV were 53.13%and 62.50%(20/32),respectively.Twenty-seven PVs were isolated,three highly variable strains of the type 1 vaccine,with seven nucleotide substitutions in the VP1 region,compared with the type 1 Sabin strains.Eighty-seven strains of NPEV were isolated and nine serotypes were identified,among which coxsackievirus B3(CVB3),echovirus 6(E6),and echovirus 11(E11)were the dominant strains.The filtration method using an MCE membrane is more sensitive than two-phase separation and can be used as a robust,sensitive,and cost-effective method to isolate enteroviruses from sewage. | Ling Fang Meizhong Chen Shuangli Zhu Wei Zhang Dongmei Yan Xiaolei Li Shufen Huang Caixia Li Xue Guo Hanri Zeng Bixia Ke Hui Li Wenbo Xu Changwen Ke Xiaoling Deng Yong Zhang Huanying Zheng | 2023 | Biosafety and Health2023，5，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员