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| 1 | A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein显示文摘最新新兴的中东呼吸症候群 coronavirus (MERS-CoV ) 能在人引起严重、致命的急性呼吸疾病。尽管有全球努力,潜力为一联系在未来流行不能被排除。有效相对的措施的发展是迫切的。MERS-CoV-specific 抗病毒的药或疫苗是还没可得到的。使用 MERS-CoV (MERS-RBD ) 的尖铁受体绑定领域使老鼠免疫,我们识别了二抵销 monoclonal 抗体(mAbs ) 4C2 和 2E6。两 mAbs potently 与高功效在 vitro 绑在 MERS-RBD 和块病毒入口。我们进一步由使在 Fab 碎片和 RBD 之间的建筑群结晶调查了他们中立化的机制,并且解决了 4C2 Fab/MERS-RBD 建筑群的结构。结构证明 4C2 认出部分重叠的 epitope 在 MERS-RBD 的受体绑定脚印,从而由位的阻碍者和接口残余竞争防碍病毒 / 受体相互作用。2E6 也堵住受体绑定,并且为绑定与 4C2 竞争到 MERS-RBD。基于结构,我们进一步由保存仅仅 paratope 残余并且从人的免疫球蛋白与对应物代替留下的氨基酸使 4C2 人性化。人性化的 4C2 (4C2h ) 抗体支撑了类似的抵销的活动和生物化学的特征到父母老鼠抗体。最后,我们证明 4C2h 能显著地在感染 MERS-CoV 的 Ad5-hCD26-transduced 鼠标的肺消除病毒 titers,因此在临床的设置为预防和治疗代表一个有希望的代理人。 | Yan Li YuhuaWan Peipei Liu Jincun Zhao Guangwen Lu Jianxun Qi Qihui Wang Xuancheng LU Ying Wu Weniun Liu Buchang Zhang Kwok-Yung Yuen Stanley Perlman George F Gao Jinghua Yan | 2015 | Cell Research2015，25，11： | 14 |
| 2 | Comparison of CMAS corrosion and sintering induced microstructural characteristics of APS thermal barrier coatings显示文摘The microstructural features of high-temperature sintered and CaO-MgO-Al_2O_3-SiO_2(CMAS) corroded air plasma sprayed Y_2O_3 stabilized ZrO_2(YSZ) thermal barrier coatings(TBCs) under the thermal gradient condition were comparatively studied. As-sprayed YSZ has a lamellar structure and the lamellae are composed of closely aligned columnar crystals. The sintered and the CMAS corroded YSZ coatings maintain the t'-ZrO_2 phase as the as-sprayed YSZ coating. The sintered YSZ remains the lamellar structure with reduced interlamellar gaps and grains coarsening. After the CMAS corrosion, the top layer of the YSZ coating keeps its lamellar structure consisting of some columnar grains with the CMAS infiltration into the intergrain gaps and the formation of striped Zr_2Y_2 O_7. The typical lamellar structure transforms into more equiaxed grains in the middle and bottom layers of the ceramic coating along with significant infiltration of amorphous CMAS and anorthite formation in the bottom layer owing to the high contents of Ca and Al. | Yiyou Wu Hua Luo Canying Cai Yanguo Wang Yichun Zhou Li Yang Guangwen Zhou | 2019 | Journal of Materials Science & Technology2019，35，3： | 5 |
| 3 | Preparation of polypyrrole/TiO2 nanocomposites with enhanced photocatalytic performance显示文摘 | Fei Gao, Xianhui Hou Aolan Wang Guangwen Chu Wei Wu Jianfeng Chen Haikui Zou | 2016 | Particuology2016，14，3： | 4 |
| 4 | Hydrogen sulfide removal by catalytic oxidative absorption method using rotating packed bed reactor显示文摘Using catalytic oxidative absorption for H_2S removal is of great interest due to its distinct advantages. However,traditional scrubbing process faces a great limitation in the confined space. Therefore, there is an urgent demand to develop high-efficiency process intensification technology for such a system. In this article, H_2S absorption experimental research was conducted in a rotating packed bed(RPB) reactor with ferric chelate absorbent and a mixture of N_2 and H_2S, which was used to simulate natural gas. The effects of absorbent p H value, gas–liquid ratio, gravity level of RPB, absorption temperature and character of the packing on the desulfurization efficiency were investigated. The results showed that H_2S removal efficiency could reach above 99.6% under the most of the experimental condition and above 99.9% under the optimal condition. A long-time continuous experiment was conducted to investigate the stability of the whole process combining absorption and regeneration. The result showed that the process could well realize simultaneous desulfurization and absorbent regeneration, and the H_2S removal efficiency kept relatively stable in the whole duration of 72 h. It can be clearly seen that high gravity technology desulfurization process, which is simple, high-efficiency, and space intensive, has a good prospect for industrial application of H_2S removal in confined space. | Liangliang Zhang Shuying Wu Zuozhong Liang Hong Zhao Haikui Zou Guangwen Chu | 2017 | Chinese Journal of Chemical Engineering2017，25，2： | 3 |
| 5 | Pyrolysis characteristics of waste tire particles in fixed-bed reactor with internals显示文摘This study investigated the characteristics of pyrolysis for waste tire particles in the newly developed fixed-bed reactor with internals that are a central gas collection channel mounted inside reactor.And a few metallic plates vertically welded on the internal wall of the reactors and extending to the region closing their central gas collection pipe walls.Experiments were conducted in two laboratory fixed bed reactors with or without the internals.The results shown that employing internals produced more light oil at externally heating temperatures above 700℃due to the inhibited secondary reactions in the reactor.The oil from the reactor with internals contained more aliphatic hydrocarbons and fewer aromatic hydrocarbons,leading to its higher H/C atomic ratios as for crude petroleum oil.The char yield was relatively stable for two beds and showed the higher heating values(HHVs)of about 23 MJ/kg.The gaseous product of pyrolysis mainly consisted of H2 and CH4,but the use of internals led to less pyrolysis gas through its promotion of oil production. | Shipei Xu Dengguo Lai Xi Zeng Lijuan Zhang Zhennan Han Jiguang Cheng Rongcheng Wu Ondřej Mašek Guangwen Xu | 2018 | Carbon Resources Conversion2018，1，3： | 3 |
| 6 | Optimizing write operation on replica in data grid显示文摘Replication technique can improve access performance and data availability of data grid systems. So far,most related work on data replication in data grid systems focuses on read-only dataset.Though write operations have been widely used in practice,its optimization problem has not been suciently studied.In this paper,we show that write requests from users can be processed by a nearest site,regardless of the location of latest written replicas.The policy of write nearby instead of write to the latest replica can improve the performance of write operations,while inconsistency is caused between multiple replicas.Therefore,we propose two metadata management algorithms to make sure that users can retrieve up-to-date datasets at any time.We extend OptorSim to support the implementation and evaluation of our scheme.The results of simulation show that our scheme achieves performance gains up to 3.3 compared with WriteMaster scheme. | XU PengZhi WU YongWei HUANG XiaoMeng YANG GuangWen ZHENG WeiMin | 2011 | Science China(Information Sciences)2011，54，1： | 2 |
| 7 | Mapping and validation of the epistatic D and P genes controlling anthocyanin biosynthesis in the peel of eggplant (Solanum melongena L.) fruit显示文摘Fruit color is an important trait inf luencing the commercial value of eggplant fruits.Three dominant genes(D,P and Y)cooperatively control the anthocyanin coloration in eggplant fruits,but none has been mapped.In this study,two white-fruit accessions(19141 and 19147)and their F2 progeny,with 9:7 segregation ratio of anthocyanin pigmented versus non-pigmented fruits,were used for mapping the D and P genes.A high-density genetic map was constructed with 5270 SNPs spanning 1997.98 cM.Three QTLs were identified,including two genes on chromosome 8 and one on chromosome 10.Gene expression analyses suggested that the SmANS on chromosome 8 and SmMYB1 on chromosome 10 were the putative candidate genes for P and D,respectively.We further identified(1)a SNP leading to a premature stop codon within the conserved PLN03176 domain of SmANS in 19141,(2)a G base InDel in the promoter region leading to an additional cis-regulatory element and(3)a 6-bp InDel within the R2-MYB DNA binding domain of SmMYB1,in 19147.Subsequently,these three variations were validated by PARMS technology as related to phenotypes in the F2 population.Moreover,silencing of SmANS or SmMYB1 in the purple red fruits of F1(E3316)led to inhibition of anthocyanin biosynthesis in the peels.Conversely,overexpression of SmANS or SmMYB1 restored anthocyanin biosynthesis in the calli of 19141 and 19147 respectively.Our findings demonstrated the epistatic interactions underlying the white color of eggplant fruits,which can be potentially applied to breeding of eggplant fruit peel color. | Qian You Huimin Li Jun Wu Tao Li Yikui Wang Guangwen Sun Zhiliang Li Baojuan Sun | 2023 | Horticulture Research2023，10，2： | 2 |
| 8 | A Novel Route to Prepare Nanocomposites in Larger Scale显示文摘准备 nanocomposites 的一条新奇线路通过润滑油的 preparingoverbased 钙石油 sulfonate 被说明净化在旋转 packedbed ( RPB )代替常规反应 vessel.The 被用作反应堆的地方,结果证明 thecarbonation 效率被改进,原料消费被减少,并且 thedispersibilities ,在 overbased 的 缩放nano 的 CaCO3 粒子的尺寸和形态学净化的 areenhanced.It 相当被推出这条线路能是广泛地 | Wei WU Lailong LUO Guangwen CHU Shengjun BAI Haikui ZOU Jianfeng CHEN | 2007 | Journal of Materials Science & Technology2007，23，3： | 2 |
| 9 | Hydrogenoysis d cellulose to C4-C7 alcohols over bi-functional CuO-MO/Al2O3 (M =Ce,Mg,Mn,Ni,Zn)catalysts coupled with methanol rdorming reaction显示文摘 | Wu Yardhua Gu Fangna Xu Guangwen | | 0，，： | 1 |
| 10 | Low levels of neutralizing antibodies against XBB Omicron subvariants after BA.5 infection显示文摘The COVID-19 response strategies in Chinese mainland were recently adjusted due to the reduced pathogenicity and enhanced infectivity of Omicron subvariants.In Chengdu,China,an infection wave was predominantly induced by the BA.5 subvariant.It is crucial to determine whether the hybrid anti-SARS-CoV-2 immunity following BA.5 infection. | Jingyun Yang Weiqi Hong Hong Lei Cai He Wenwen Lei Yanan Zhou Tingmei Zhao Aqu Alu Xuelei Ma Jiong Li Li Yang Zhenling Wang Wei Wang Guangwen Lu Guobo Shen Shuaiyao Lu Guizhen Wu Huashan Shi Xiawei Wei | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 1 |
| 11 | A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants显示文摘There are currently approximately 4000 mutations in the SARS-CoV-2 S protein gene and emerging SARS-CoV-2 variants continue to spread rapidly worldwide.Universal vaccines with high efficacy and safety urgently need to be developed to prevent SARS-CoV-2 variants pandemic.Here,we described a novel self-assembling universal mRNA vaccine containing a heterologous receptorbinding domain(HRBD)-based dodecamer(HRBD^(dodecamer))against SARS-CoV-2 variants,including Alpha(B.1.1.7),Beta(B.1.351),Gamma(B.1.1.28.1),Delta(B.1.617.2)and Omicron(B.1.1.529).HRBD containing four heterologous RBD(Delta,Beta,Gamma,and Wild-type)can form a stable dodecameric conformation under T4 trimerization tag(Flodon,FD).The HRBD^(dodecamer)-encoding mRNA was then encapsulated into the newly-constructed LNPs consisting of a novel ionizable lipid(4N4T).The obtained universal mRNA vaccine(4N4T-HRBD^(dodecamer))presented higher efficiency in mRNA transfection and expression than the approved ALC-0315 LNPs,initiating potent immune protection against the immune escape of SARS-CoV-2 caused by evolutionary mutation.These findings demonstrated the first evidence that structure-based antigen design and mRNA delivery carrier optimization may facilitate the development of effective universal mRNA vaccines to tackle SARS-CoV-2 variants pandemic. | Shugang Qin Hai Huang Wen Xiao Kepan Chen Xi He Xiaoshan Tang Zhiying Huang Yupei Zhang Xing Duan Na Fan Qian Zheng Min Wu Guangwen Lu Yuquan Wei Xiawei Wei Xiangrong Song | 2023 | Acta Pharmaceutica Sinica B2023，13，10： | 1 |
| 12 | Hydrogenolysis of cellulose to C4-C7alcohols over bi-functional Cu O-MO/Al2O3(M=Ce,Mg,Mn,Ni,Zn)catalysts coupled with methanol reforming reaction显示文摘 | WU Yanhua GU Fangna XU Guangwen | 2013 | Bioresour Technol2013，137，： | 1 |
| 13 | Grid Scheduling Based on Prediction of Task Completion Time显示文摘 | WU Yongwei CHEN Gang YANG Guangwen ZHENG Weimin | 2009 | Chinese Journal of Electronics2009，18，3： | 1 |
| 14 | Experi- mental study on carboxymethyl chitosan in preventing peritoneal adhesion in rats显示文摘 | Zhang Ziping Zhou Guangwen Wu Huiping | 2000 | J Clin Res Jun2000，27，6： | 1 |
| 15 | Hydrogenolysis of cellulose to C4–C7 alcohols over bi-functional CuO–MO/Al 2 O 3 (M=Ce, Mg, Mn, Ni, Zn) catalysts coupled with methanol reforming reaction显示文摘 | Yanhua Wu Fangna Gu Guangwen Xu Ziyi Zhong Fabing Su | 2013 | Bioresource Technology2013，，： | 1 |
| 16 | Direct synthesis of diphenyl carbonate over heterogeneous catalyst: effects of structure of substituted perovskite carrier on the catalyst activities显示文摘The perovskite-type compound LaMnO_(3) was substituted for the part of La in position A and for the part of Mn in position B by citrate method.The phases were detected by X-ray diffraction.Powder morphologies were scanned by scanning electron microscopy.The valence of atoms was determined by X-ray photoelectron spectroscopy.It was found that the perovskite can form crystal defect and increase the proportion of high valence B element by doping.Active component Pd was loaded on various perovskite supports for synthesis of diphenyl carbonate.The results showed that the activities of catalysts in which supports have crystal defect by substitution were higher.It can be concluded that perovskite with defect structure by doping could lead to the formation of oxygen vacancy where the lattice oxygen became exchangeable with the oxygen gas.Also,this improved the redox process of the carrier by transferring electrons and activities of catalysts. | WU Guangwen WU Yuxin MA Peisheng JIN Fang ZHANG Guangxu LI Dinghuo WANG Cunwen | 2007 | Frontiers of Chemical Science and Engineering2007，1，1： | 1 |
| 17 | Hydrogenolysis of cellulose to C4-C7 alcohols over bifunctional CuO-MO/A120s (M=Ce, Mg, Mn, Ni, Zn) catalysts coupled with methanol reforming reaction 显示文摘 | Wu Yanhua Gu Fangna Xu Guangwen | 2013 | Bioresource Technology2013，，137： | 1 |
| 18 | NOx storage-reduction over Pt/Mg-Al-O catalysts with different Mg/Al atomic ratios显示文摘 | Hao Cheng Guangwen Chen Shudong Wang Diyong Wu Yin Zhang Henqiang Li | 2004 | Korean Journal of Chemical Engineering2004，，3： | 1 |
| 19 | Preliminary understanding of initial reaction process for subbituminous coal pyrolysis with molecular dynamics simulation显示文摘 | Jin-Hui Zhan Rongcheng Wu Xiaoxing Liu Shiqiu Gao Guangwen Xu | 2014 | Fuel2014，，： | 1 |
| 20 | Hepatitis B Virus Combo Mutations Improve the Prediction and Active Prophylaxis of Hepatocellular Carcinoma: A Clinic-Based Cohort Study显示文摘 | Jianhua Yin Junxue Wang Rui Pu Haiguang Xin Zixiong Li Xue Han Yibo Ding Yan Du Wenbin Liu Yang Deng Xiaowei Ji Ming Wu Min Yu Hongwei Zhang Hongyang Wang Timothy C. Thompson Wu Ni Guangwen Cao | 2015 | Cancer Prevention Research2015，，： | 1 |
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