|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Systemic immune-inflammation index for predicting prognosis of colorectal cancer显示文摘AIM To investigate the clinical significance of preoperative systemic immune-inflammation index(SII) in patients with colorectal cancer(CRC). METHODS A retrospective analysis of 1383 cases with CRC was performed following radical surgery. SII was calculated with the formula SII =(P × N)/L, where P, N, and L refer to peripheral platelet, neutrophil, and lymphocyte counts, respectively. The clinicopathological features and follow-up data were evaluated to compare SII with other systemic inflammation-based prognostic indices such as the neutrophil-lymphocyte ratio(NLR) and platelet-lymphocyte ratio(PLR) in patients with CRC.RESULTS The optimal cut-off point for SII was defined as 340. The overall survival(OS) and disease-free survival(DFS) were better in patients with low NLR, PLR, and SII(P < 0.05). The SII was an independent predictor of OS and DFS in multivariate analysis. The area under the receiver-operating characteristics(ROC) curve for SII(0.707) was larger than those for NLR(0.602) and PLR(0.566). In contrast to NLR and PLR, SII could effectively discriminate between the TNM subgroups. CONCLUSION SII is a more powerful tool for predicting survival outcome in patients with CRC. It might assist the identification of high-risk patients among patients with the same TNM stage. | Jian-Hui Chen Er-Tao Zhai Yu-Jie Yuan Kai-Ming Wu Jian-Bo Xu Jian-Jun Peng Chuang-Qi Chen Yu-Long He Shi-Rong Cai | 2017 | World Journal of Gastroenterology2017，23，34： | 79 |
| 2 | Spectrum and antimicrobial resistance of common pathogenic bacteria isolated from patients with acute exacerbation of chronic obstructive pulmonary disease in mainland of China显示文摘 | YE Feng HE Li-xian CAI Bo-qiang WEN Fu-qiang CHEN Bai-yi Mangunnegoro Hadiarto CHEN Rong-chang YUAN Jin-ping SUN Hong-li | 2013 | Chinese Medical Journal2013，，12： | 55 |
| 3 | Colorectal cancer lymph node staining by activated carbon nanoparticles suspension in vivo or methylene blue in vitro显示文摘AIM:To investigate whether activated carbon nanoparticles suspension(ACNS) or methylene blue(MB) can increase the detected number of lymph nodes in colorectal cancer.METHODS:Sixty-seven of 72 colorectal cancer patients treated at our hospital fulfilled the inclusion criteria of the study which was conducted from December 2010 to February 2012.Seven patients refused to participate.Eventually,60 patients were included,and randomly assigned to three groups(20 in each group):ACNS group(group A),MB group(group B) and non-stained conventional surgical group(group C).In group A,patients received subserosal injection of 1 mL ACNS in a 4-quadrant region around the mass.In group B,the main artery of specimen was identified and isolated after the specimen was removed,and 2 mL MB was slowly injected into the isolated,stretched and fixed vessel.In group C,no ACNS and MB were injected.All the mesentery lymph nodes were isolated and removed systematically by visually inspecting and palpating the adipose tissue.RESULTS:No difference was observed among the three groups in age,gender,tumor location,tumor diameter,T-stage,degree of differentiation,postoperative complications and peritoneal drainage retention time.The total number of detected lymph nodes was 535,476 and 223 in the three groups,respectively.The mean number of detected lymph nodes per patient was significantly higher in group A than in group C(26.8 ± 8.4 vs 12.2 ± 3.2,P < 0.001).Similarly,there were significantly more lymph nodes detected in group B than in group C(23.8 ± 6.9 vs 12.2 ± 3.2,P < 0.001).However,there was no significant difference between group A and group B.There were 50,46 and 32 metastatic lymph nodes dissected in 13 patients of group A,10 patients of group B and 11 patients of group C,without significant differences among the three groups.Eleven of the 60 patients had insufficient number of detected lymph nodes(< 12).Only one patient with T 4a rectal cancer had 10 lymph nodes detected in group B,the other 10 patients were all from group C.Based on the different diameter categories,the number of detected lymph nodes in groups A and B was significantly higher than in group C.However,there was no statistically significant difference between group A and group B.The metastatic lymph nodes were not significant different among the three groups.Similarly,tumor location,T stage and tumor differentiation did not affect the staining results.Body mass index was a minor influencing factor in the two different staining methods.The stained lymph nodes can easily be identified from the mesenteric adipose tissues,and the staining time for lymph nodes was not significantly different compared with unstained group.None of the patients in groups A and B had drug-related complications.CONCLUSION:Both activated carbon nanoparticles suspension in vivo and methylene blue in vitro can be used as tracers to increase the detected number of lymph nodes in colorectal cancer. | Hong-Ke Cai Hai-Fei He Wei Tian Mei-Qi Zhou Yue Hu Yong-Chuan Deng | 2012 | World Journal of Gastroenterology2012，18，42： | 39 |
| 4 | Analysis of Factors Associated with Death in Maintenance Hemodialysis Patients: A Multicenter Study in China显示文摘 | Kang-Kang Song De-Long Zhao Yuan-Da Wang Yong Wang Xue-Feng Sun Li-Ning Miao Zhao-Hui Ni Hong-Li Lin Fu-You Liu Ying Li Ya-Ni He Nian-Song Wang Cai-Li Wang Ai-Hua Zhang Meng-Hua Chen Xiao-Ping Yang Yue-Yi Deng Feng-Min Shao Shu-Xia Fu Jing-Ai Fang Guang-Yan Cai Xiang-Mei Chen | 2017 | Chinese Medical Journal2017，，8： | 39 |
| 5 | Toll-like receptor 4/nuclear factor-kappa B signaling detected in brain after early subarachnoid hemorrhage显示文摘背景发炎和免疫在 subarachnoid 出血(SAH ) 以后在早大脑损害的致病起一个重要作用。原子 factor-kappa B (NF-KB ) 为发炎和免疫调整许多基因必需品并且被像使用费的受体(TLR ) 激活。这研究试图检测在早 SAH 以后在老鼠大脑发信号的像使用费的受体 4/nuclear factor-kappa B (TLR4/NF-KB ) 的表示。老鼠被将斩首的方法和他们的大脑在血的单个注射以后在 0, 2, 4, 6, 12, 24 和 48 个小时被移开进 prechiasmatic 水塘, TLR4 的 mRNA 表示被 immunohistochemistry 并且西方的弄污被 Taqman 即时 RT-PCR,和蛋白质表示测量。肿瘤坏死 factor-alpha (TNF-) 的 NF-KB 活动和集中, interleukin-lbeta (IL-) 和 intedeukin-6 (IL-6 ) 被试金(ELISA ).Results TaqMan 即时 RT-PCR 并且西方的弄污识别了的连接酶的 immunosorbent 测量在在 mRNA 和蛋白质的 TLR4 表示的一个 biphasic 变化:一座起始的山峰(2-6 小时) 和持续举起(12-48 小时) 。染色的 Immunohistochemical 在 glial 房间和脉管的内皮细胞层主要显示出 TLR4-1ike 免疫反应的可诱导的表示。在象 NF-KB-regulated proinflammatory cytokines (TNF- , IL-1 和 IL-6 ) 的生产一样的 NF-KB 子单元 p65 的激活的一个类似的 biphasic 变化被 ELISA 检测。结论这些数据建议试验性的 SAH 导致在早大脑损害发信号的 TLR4 表示和 NF-KB 的重要起来规定。发信号的 TLR4/NF-KB 的激活可以在 SAH 以后调整煽动性的回答。 | MA Chun-xiao YIN Wei-ning CAI Bo-wen WU Jian WANG Jun-yi HE Min SUN Hong DING Jun-li YOU Chao | 2009 | Chinese Medical Journal2009，，13： | 33 |
| 6 | Methylophiopogonanone A suppresses ischemia/ reperfusion-induced myocardial apoptosis in mice via activating PI3K/Akt/eNOS signaling pathway显示文摘瞄准:Ophiopogon japonicus 的弄干的 tuber 根为心肌的局部缺血和血栓的处理在繁体中文药被使用了。在这研究,我们调查了 methylophiopogonanone A (恐鸟) 的效果,在 Ophiopogon japonicus 的主要 homoisoflavonoid,在心肌的 ischemia/reperfusion (I/R ) 上 injury.Methods:老鼠是有恐鸟的 pretreated (10 mg· kg -1·d-1, po ) 为 2 个星期然后使遭到了到左前面的下降冠的动脉的短暂吸藏。心脏的功能被评估,并且梗塞尺寸和 apoptosis 索引被估计。位于恐鸟的 cardio 保护下面的机制在受到 hypoxia/reoxygenation (H/R ) 的 H9C2 老鼠 cardiomyocytes 被分析。房间生存能力和 apoptosis 被评估;apoptotic 和相关发信号蛋白质被分析。在文化媒介的层次都不是 assessed.Results:在 I/R 鼠标,有恐鸟的预告的处理显著地减少了梗塞尺寸(在 60.7%) 并且心肌的 apoptosis (在 56.8%) ,并且改进心脏的功能。在受到 H/R 的 H9C2 房间,有恐鸟的预告的处理(10 μ mol/L ) 显著地减少的 apoptosis 和劈开的 caspase-3 表示,提高了 Bcl-2/Bax 比率并且没恢复生产。而且,有恐鸟的预告的处理显著地在受到 H/R 的 H9C2 房间增加了 PI3K/Akt/eNOS 小径的激活,并且恐鸟的保护的效果面对 PI3K 禁止者 wortmannin (100 nmol/L ) 被废除 .Conclusion:恐鸟稀释 I/R-induced 在经由激活发信号的 PI3K/Akt/eNOS 的老鼠的心肌的 apoptosis 小径。 | Fei HE Bang-long XU Cai CHEN Hong-jing JIA Ji-xiong WU Xiao-chen WANG Jian-long SHENG Li HUANG Jing CHENG | 2016 | Acta Pharmacologica Sinica2016，37，6： | 32 |
| 7 | Targeting PI3K/AKT signaling for treatment of idiopathic pulmonary fibrosis显示文摘Idiopathic pulmonary fibrosis(IPF) is a chronic progressive fibrotic interstitial pneumonia with unknown causes. The incidence rate increases year by year and the prognosis is poor without cure.Recently, phosphatidylinositol 3-kinase(PI3 K)/protein kinase B(PKB/AKT) signaling pathway can be considered as a master regulator for IPF. The contribution of the PI3 K/AKT in fibrotic processes is increasingly prominent, with PI3 K/AKT inhibitors currently under clinical evaluation in IPF. Therefore,PI3 K/AKT represents a critical signaling node during fibrogenesis with potential implications for the development of novel anti-fibrotic strategies. This review epitomizes the progress that is being made in understanding the complex interpretation of the cause of IPF, and demonstrates that PI3 K/AKT can directly participate to the greatest extent in the formation of IPF or cooperate with other pathways to promote the development of fibrosis. We further summarize promising PI3 K/AKT inhibitors with IPF treatment benefits, including inhibitors in clinical trials and pre-clinical studies and natural products, and discuss how these inhibitors mitigate fibrotic progression to explore possible potential agents, which will help to develop effective treatment strategies for IPF in the near future. | Jincheng Wang Kaili Hu Xuanyan Cai Bo Yang Qiaojun He Jiajia Wang Qinjie Weng | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 31 |
| 8 | (-)-Epigallocatechin-3-gallate inhibits growth of gastric cancer by reducing VEGF production and angiogenesis显示文摘AIM: To investigate the effect of (-)-epigallocatechin- 3-gallate (EGCG) on growth of gastric cancer and its possible mechanism. METHODS: Heterotopic tumors were induced by subcutaneously injection of SGC-7901 cells in nude mice. Tumor growth was measured by calipers in two dimensions. Tumor angiogenesis was determined with tumor microvessel density (MVD) by immunohistology. Vascular endothelial growth factor (VEGF) protein level and activation of signal transducer and activator of transcription 3 (Stat3) were examined by Western blotting. VEGF mRNA expression was determined by RT-PCR and VEGF release in tumor culture medium by ELISA. VEGF-induced cell proliferation was studied by MTT assay, cell migration by gelatin modified Boyden chamber (Transwell) and in vitro angiogenesis by endothelial tube formation in Matrigel. RESULTS: Intraperitoneal injection of EGCG inhibited the growth of gastric cancer by 60.4%. MVD in tumor tissues treated with EGCG was markedly reduced. EGCG treatment reduced VEGF protein level in vitro and in vivo. Secretion and mRNA expression of VEGF in tumor cells were also suppressed by EGCG in a dose-dependent manner. This inhibitory effect was associated with reduced activation of Stat3, but EGCG treatment did not change the total Stat3 expression. EGCG also inhibited VEGF-induced endothelial cell proliferation, migration and tube formation. CONCLUSION: EGCG inhibits the growth of gastriccancer by reducing VEGF production and angiogenesis, and is a promising candidate for anti-angiogenic treatment of gastric cancer. | Bao-He Zhu, Wen-Hua Zhan, Zheng-Rong Li, Zhao Wang, Yu-Long He, Jun-Sheng Peng, Shi-Rong Cai, Jin-Ping Ma, Chang-Hua Zhang, Department of Gastrointestinal & Pancreatic Surgery, First Affiliated Hospital, Sun Yat-Sen University Gastric Center of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China | 2007 | World Journal of Gastroenterology2007，13，8： | 30 |
| 9 | Prognostic value of systemic immune.inflammation index in patients with gastric cancer显示文摘Background:Inflammation-based indexes have been used to predict survival and recurrence in cancer patients.Systemic immune-inflammation index(Sll) was reported to be associated with prognosis in some malignant tumors.In the present study,we aimed to explore the association between Sll and the prognosis of patients with gastric cancer.Methods:We retrospectively analyzed data from 444 gastric cancer patients who underwent gastrectomy at the First Affiliated Hospital of Sun Yat-sen University between January 1994 and December 2005.Preoperative Sll was calculated.The Chi square test or Fisher's exact test was used to determine the relationship between preoperative Sll and clinicopathologic characteristics.Overall survival(OS) rates were estimated using the Kaplan-Meier method,and the effect of Sll on OS was analyzed using the Cox proportional hazards model.Receiver operating characteristic(ROC)curves were used to compare the predictive ability of Sll,NLR,and PLR.Results:Sll equal to or higher than 660 was significantly associated with old age,large tumor size,unfavorable Borrmann classification,advanced tumor invasion,lymph node metastasis,distant metastasis,advanced TNM stage,and high carcino-embryonic antigen level,high neutrophil-lymphocyte ratio,and high platelet-lymphocyte ratio(all P<0.05).High Sll was significantly associated with unfavorable prognosis(P<0.001) and Sll was an independent predictor for OS(P=0.015).Subgroups analysis further showed significant associations between high Sll and short OS in stage Ⅰ,Ⅱ,Ⅲ subgroups(all P<0.05).Sll was superior to NLR and PLR for predicting OS in patients with gastric cancer.Conclusion:Preoperative Sll level is an independent prognostic factor for OS in patients with gastric cancer. | Kang Wang Feiyu Diao Zhijun Ye Xinhua Zhang Ertao Zhai Hui Ren Tong Li Hui Wu Yulong He Shirong Cai Jianhui Chen | 2017 | Chinese Journal of Cancer2017，36，9： | 30 |
| 10 | Denitrification Losses and N_2O Emissions from Nitrogen Fertilizer Applied to a Vegetable Field显示文摘A field experiment was conducted on Chinese cabbage (Brassica campestris L. ssp. pekinensis (Lour.) Olsson) in a Nanjing suburb in 2003. The experiment included 4 treatments in a randomized complete block design with 3 replicates: zero chemical fertilizer N (CK); urea at rates of 300 kg N ha-1 (U300) and 600 kg N ha-1 (U600), both as basal and two topdressings; and polymer-coated urea at a rate of 180 kg N ha-1 (PCU180) as a basal application. The acetylene inhibition technique was used to measure denitrification (N2+N2O) from intact soil cores and N2O emissions in the absence of acetylene. Results showed that compared to CK total denitrification losses were significantly greater (P≤0.05) in the PCU180, U300, and U600 treatments, while N2O emissions in the U300 and U600 treatments were significantly higher (P≤0.05) than CK. In the U300 and U600 treatments peaks of denitrification and N2O emission were usually observed after N application. In the polymer-coated urea treatment (PCU180) during the period 20 to 40 days after transplanting, higher denitrification rates and N2O fluxes occurred. Compared with urea, polymer-coated urea did not show any effect on reducing denitrification losses and N2O emissions in terms of percentage of applied N. As temperature gradually decreased from transplanting to harvest, denitrification rates and N2O emissions tended to decrease. A significant (P≤0.01) positive correlation occurred between denitrification (r = 0.872) or N2O emission (r = 0.781) flux densities and soil temperature in the CK treatment with a stable nitrate content during the whole growing season. | CAO Bing He Fa-Yun Xu Qiu-Ming Yin Bin CAI Gui-Xin | 2006 | Pedosphere2006，16，3： | 28 |
| 11 | Basic Soil Productivity of Spring Maize in Black Soil Under Long-Term Fertilization Based on DSSAT Model显示文摘Increasing basic farmland soil productivity has significance in reducing fertilizer application and maintaining high yield of crops. In this study, we defined that the basic soil productivity(BSP) is the production capacity of a farmland soil with its own physical and chemical properties for a specific crop season under local environment and field management. Based on 22-yr(1990-2011) long-term experimental data on black soil(Typic hapludoll) in Gongzhuling, Jilin Province, Northeast China, the decision support system for an agro-technology transfer(DSSAT)-CERES-Maize model was applied to simulate the yield by BSP of spring maize(Zea mays L.) to examine the effects of long-term fertilization on changes of BSP and explore the mechanisms of BSP increasing. Five treatments were examined:(1) no-fertilization control(control);(2) chemical nitrogen, phosphorus, and potassium(NPK);(3) NPK plus farmyard manure(NPKM);(4) 1.5 time of NPKM(1.5NPKM) and(5) NPK plus straw(NPKS). Results showed that after 22-yr fertilization, the yield by BSP of spring maize significantly increased 78.0, 101.2, and 69.4% under the NPKM, 1.5NPKM and NPKS, respectively, compared to the initial value(in 1992), but not significant under NPK(26.9% increase) and the control(8.9% decrease). The contribution percentage of BSP showed a significant rising trend(P<0.05) under 1.5NPKM. The average contribution percentage of BSP among fertilizations ranged from 74.4 to 84.7%, and ranked as 1.5NPKM>NPKM>NPK≈NPKS, indicating that organic manure combined with chemical fertilizers(1.5NPKM and NPKM) could more effectively increase BSP compared with the inorganic fertilizer application alone(NPK) in the black soil. This study showed that soil organic matter(SOM) was the key factor among various fertility factors that could affect BSP in the black soil, and total N, total P and/or available P also played important role in BSP increasing. Compared with the chemical fertilization, a balanced chemical plus manure or straw fertilization(NPKM or NPKS) not only increased the concentrations of soil nutrient, but also improved the soil physical properties, and structure and diversity of soil microbial population, resulting in an iincrease of BSP. We recommend that a balanced chemical plus manure or straw fertilization(NPKM or NPKS) should be the fertilization practices to enhance spring maize yield and improve BSP in the black soil of Northeast China. | ZHA Yan WU Xue-ping HE Xin-hua ZHANG Hui-min GONG Fu-fei CAI Dian-xiong ZHU Ping GAO Hong-jun | 2014 | Journal of Integrative Agriculture2014，13，3： | 25 |
| 12 | Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits显示文摘Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06. | WANG Gang PAN Junsong LI Xiaozun HE Huanle WU Aizhong CAI Run | 2005 | Science China(Life Sciences)2005，48，3： | 24 |
| 13 | Cloud point extraction coupled with HPLC-UV for the determination of phthalate esters in environmental water samples显示文摘A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate (DEP), di-(2-ethylhexyl)-phthalate (DEHP) and di-cyclohexyl-phthalate (DCP) in environmental water samples using high-performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as extraction solvent. The parameters affecting extraction effciency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, the method can achieve preconcentration factors of 35, 88, 111 and detection of limits of 2.0, 3.8, 1.0 ngml for DEP, DEHP and DCP in 10-ml water sample, respectively. The proposed method was successfully applied to the determination of trace amount of phathalate esters in effuent water of the wastewater treatment plant and the lixivium of plastic fragments. | WANG Ling JIANG Gui-bin CAI Ya-qi HE Bin WANG Ya-wei SHEN Da-zhong | 2007 | Journal of Environmental Sciences2007，19，7： | 23 |
| 14 | Effects of Long-Term Application of Inorganic Fertilizers on Biochemical Properties of a Rice-Planting Red Soil显示文摘一个长期的实验在江西省的 Yingtan 被建立在从第四级的红壤导出的种大米的土壤的生物化学的性质上调查无机肥料的长期的申请的效果。Noncultivated 土壤被低 pH 和缺乏极其在可得到的营养素,特别地 P 和 N 侵蚀并且描绘。在 13 年在为双 cropped 大米的耕作的无机的授精以后,土壤的生物化学的性质被改变。没有 N 申请,氮的硝化作用潜力和脲酶活动比那些在有 N 申请的处理是更高的。没有 P 申请,酸性磷酸酶活动和脱氢酶活动比在那些在有 P 申请的处理也是更高的。脱氢酶活动与全部的 P 和 hydrolysable N 的集中并且与米饭庄稼产量相关很好,建议那项脱氢酶活动可能是为在土壤肥力的改进的合适的指示物。 | ZHONG Wen-Hui CAI Zu-Cong ZHANG He | 2007 | Pedosphere2007，17，4： | 22 |
| 15 | Immune response,stress resistance and bacterial challenge in juvenile rainbow trouts Oncorhynchus mykiss fed diets containing chitosan-oligosaccharides显示文摘Effects of dietary supplementation of chitosan-oligosaccharides(COS)on the growth performance,immune response,stress resistance,and disease resistance of juvenile rainbow trout Oncorhynchus mykiss were studied.Four experimental diets containing 0,20,40,or 60 mg/kg COS(COS0,COS20,COS40,and COS60,respectively)were fed to juvenile rainbow trout(initial weight=5.2±0.3 g)for 8 weeks.By the end of the feeding trial,representative groups of fish from each dietary treatment were challenged with stressor(30 sec air exposure)and pathogen exposure(intraperitoneal injection with Aeromonas hydrophila).Results showed that supplementation of COS in diets did not affect production performance and body composition of rainbow trout.However,fish fed the COS40 diet demonstrated improved phagocytic activities,respiratory burst activities and decreased serum cortisol level.Additionally,survival following A.hydrophila challenge was significant higher among fish fed the COS-supplemented feeds,although there was no difference based on the level of supplementation.The present study suggests that COS can be used as an immuno-stimulant in rainbow trout feeds. | Lin LUO Xuefeng CAI Chuan HE Min XUE Xiufeng WU Haining CAO | 2009 | Current Zoology2009，55，6： | 22 |
| 16 | (-)-Epigallocatechin-3-gallate inhibits VEGF expression induced by IL-6 via Stat3 in gastric cancer显示文摘AIM: To demonstrate that (-)-Epigallocatechin-3-gallate (EGCG) inhibits vascular endothelial growth factor (VEGF) expression and angiogenesis induced by interleukin-6 (IL-6) via suppressing signal transducer and activator of transcription 3 (Stat3) activity in gastric cancer. METHODS: Human gastric cancer (AGS) cells were treated with IL-6 (50 ng/mL) and EGCG at different concentrations. VEGF, total Stat3 and activated Stat3 protein levels in the cell lyses were examined by Western blotting, VEGF protein level in the conditionedmedium was measured by enzyme-linked immunosorbent assay, and the level of VEGF mRNA was evaluated by reverse transcription polymerase chain reaction (RTPCR). Stat3 nuclear translocation was determined by Western blotting with nuclear extract, and Stat3-DNA binding activity was examined with Chromatin immunoprecipitation (ChIP) assay. IL-6 induced endothelial cell proliferation was measured with 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazoliumbromide assay, in vitro angiogenesis was determined with endothelial cell tube formation assay in Matrigel, and IL-6-induced angiogenesis in vitro was measured with Matrigel plug assay. RESULTS: There was a basal expression and secretion of VEGF in AGS cells. After stimulation with IL-6, VEGF expression was apparently up-regulated and a 2.4-fold increase was observed. VEGF secretion in the conditioned medium was also increased by 2.8 folds. When treated with EGCG, VEGF expression and secretion were dose-dependently decreased. IL-6 also increased VEGF mRNA expression by 3.1 folds. EGCG treatment suppressed VEGF mRNA expression in a dose-dependent manner. EGCG dose-dependently inhibited Stat3 activation induced by IL-6, but did not change the total Stat3 expression. When treated with EGCG or AG490, VEGF expressions were reduced to the level or an even lower level in the tumor cells not stimulated with IL-6. However, PD98059 and LY294002 did not change VEGF expression induced by IL-6. EGCG inhibited Stat3 nucleus translocation, and Stat3-DNA binding activity was also markedly decreased by EGCG. Furthermore, EGCG inhibited IL-6 induced vascular endothelial cell proliferation and tube formation in vitro and angiogenesis in vitro . CONCLUSION: EGCG inhibits IL-6-induced VEGF expression and angiogenesis via suppressing Stat3 activity in gastric cancer, which has provided a novel mechanistic insight into the anti-angiogenic activity of EGCG. | Bao-He Zhu Hua-Yun Chen Wen-Hua Zhan Cheng-You Wang Shi-Rong Cai Zhao Wang Chang-Hua Zhang Yu-Long He | 2011 | World Journal of Gastroenterology2011，17，18： | 21 |
| 17 | Efficacy and Safety of Niaoduqing Particles for Delaying Moderate-to-severe Renal Dysfunction: A Randomized, Double-blind, Placebo-controlled, Multicenter Clinical Study显示文摘 | Ying Zheng Guang-Yan Cai Li-Qun He Hong-Li Lin Xiao-Hong Cheng Nian-Song Wang Gui-Hua Jian Xu-Sheng Liu Yu-Ning Liu Zhao-Hui Ni Jing-Ai Fang Han-Lu Ding Wang Guo Ya-Ni He Li-Hua Wang Ya-Ping Wang Hong-Tao Yang Zhi-Ming Ye Ren-Huan YU Li-Juan Zhao Wen-Hua Zhou Wen-Ge Li Hui-Juan Mao Yong-Li Zhan Zhao Hu Chen Yao Ri-Bao Wei Xiang-Mei Chen | 2017 | Chinese Medical Journal2017，，20： | 21 |
| 18 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 19 | Endoscopic Surgery versus External Ventricular Drainage Surgery for Severe Intraventricular Hemorrhage显示文摘The efficacy and applied value of endoscopic hematoma evacuation vs. external ventricular drainage (EVD) in the treatment of severe ventficular hemorrhage (IVH) were explored and compared.From Jan.2015 to Dec.2016,the clinical data of 42 cases of IVH were retrospectively analyzed,including 18 patients undergoing endoscopic hematoma evacuation (group A),and 24patients receiving EVD (group B).The hematoma clearance rate was calculated by 3D Slicer software,and complications and outcomes were compared between the two groups.There were no significant differences in age,sex and Graeb score between groups A and B (P>0.05).The hematoma clearance rate was 70.81%±27.64% in group A and 48.72%±36.58% in group B with a statistically significant difference (P<0.05).The operative time in groups A and B was 72.45±25.26 min and 28.54±15.27min,respectively (P<0.05).The Glasgow Coma Scale (GCS) score increased from 9.28±2.72 at baseline to 11.83±2.91 at 1 week postoperatively in group A,and from 8.25±2.62 at baseline to 10.79±4.12 at 1 week postoperatively in group B (P<0.05).The length of hospital stay was 12.67±5.97 days in group A and 17.33±8.91 days in group B with a statistically significant difference (P<0.05).The GOS scores at 6 months after surgery were 3.83±1.12 in group A,and 2.75±1.23 in group B (P<0.05). These results suggested that endoscopic hematoma evacuation has an advantage of a higher hematoma clearance rate,fewer complications and better outcomes in the treatment of severe IVH,indicating it is a safe,effective and promising approach for severe IVH. | Ping SONG Fa-liang DUAN Qiang CAI Jing-lei WU Xiao-bin CHEN Yuan WANG Cong-gang HUANG Ji-qiang LI Zhu-qiang HE Qiao-chun HUANG Mei LIU Yan-gao ZHANG Ming LUO | 2018 | Current Medical Science2018，38，5： | 21 |
| 20 | Clinicopathologic features and survival of patients with colorectal mucinous, signet-ring cell or non-mucinous adenocarcinoma:experience at an institution in southern China显示文摘以前的研究显示出冲突的背景与 colorectal mucinous,图章戒指房间,或 non-mucinous 腺癌在在 clinicopathologic 特征和病人的预后之间的关系上结果;仅仅这学习的很少在中国被执行了。这回顾的研究从我们的部门分析了数据调查 clinicopathologic 特征,预后和三种 histologic 类型的可能的关联-- colorectal mucinous ,图章戒指房间,和 non-mucinous 腺癌,到为可以导致 2079 个病人的指向的治疗方法的开发的观察差别的库与在 1994 和 2007 之间的 colorectal 癌症诊断了的清澈, 144 有 mucinous , 25 有的图章戒指房间,和 1837 有的 non-mucinous 腺癌。他们的 clinicopathologic 参数和幸存用确定的统计 methodologies.Results Mucinous 被分析,图章戒指房间腺癌在更年轻的病人是普通的(P < 0.001 ) 。地点,尺寸和疾病舞台在三种类型之中显著地不同。图章戒指房间肿瘤更通常比 mucinous 和 non-mucinous 腺癌在直肠被发现(P < 0.001 ) 。Mucinous 和图章戒指房间肿瘤比 non-mucinous 腺癌更经常在生活在一个以后的阶段介绍了,与淋巴节点参与, serosal 渗入,腹传播,和邻近的机关侵略(P < 0.01 ) 。激进的切除术,肝的转移和本地复发的率没在类型(P 0.05 ) 之中不同。与有 non-mucinous 腺癌的病人相比,潜在地经历了药品切除术或阶段 / 疾病的有 mucinous 和图章戒指房间肿瘤的病人有更差的长期的全面幸存。幸存没与舞台或疾病(P 0.05 ) 为病人由类型不同。结论 Mucinous 和图章戒指房间腺癌让唯一的 carcinogenesis 和类似的生物学的 behavior.Our 学习证实两 histologic 打字,特别图章戒指房间肿瘤,为有 colorectal 癌症的病人的独立、否定预示的因素。当疾病是任何一个舞台时,类型不看起来在幸存上有重要效果或在表示。 | SONG Wu WU Sui-jing HE Yu-long CAI Shi-rong ZHANG Chang-hua ZHANG Xin-hua ZHAN Wen-hua | 2009 | Chinese Medical Journal2009，，13： | 20 |
您所在的机构暂未开通此部分的功能，请联系管理员