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| 1 | Corrosionfailureanalysisofoiltanksbymeansoffaulttreeanalysis显示文摘 | Zhao Xuee JiangJuncheng Wang Ruojun | 2006 | CorrosionScienceandProtection2006，18，3： | 1 |
| 2 | Natural Polyphenol Inspired Polycatechols for Efficient siRNA Delivery显示文摘There is a continuing quest to rationally fabricate polymeric biomaterials with both high transfection efficiency and minimal toxicity for the emerging opportunities in small interfering RNA(siRNA)delivery.Recently,this goal was promoted highly by developing a robust and efficient strategy to facilitate polymer-mediated RNAi using natural polyphenols with multiple phenol groups that could condense siRNA effectively into negatively charged nanoparticles(NPs).Further coating of these NPs with cationic polymers of low molecular weight enabled their intracellular siRNA delivery.Inspired by the structural and functional features of natural polyphenols,we aimed to further the development of low molecular weight polycatechols as a new class of efficient and biocompatible polymers for siRNA delivery in our current study.The fabricated polycatechols have benefits of requiring only one-step fabrication toward efficient siRNA nanoformulations.Moreover,they could deliver siRNA into cells and silence target genes both in vitro and in vivo.The resulting polycatechol/siRNA formulations were also functionally competent,demonstrating a successful,profound downregulation of a proinflammatory enzyme to attenuate chronic intestinal inflammation in an intestinal injury model.This study provides a new approach in chemistry for the development of efficient synthetic polymers for therapeutic siRNA delivery. | Wanwan Shen Ruojun Wang Qianqian Fan Xiao Gao Hui Wang Yang Shen Yiwen Li Yiyun Cheng | 2020 | CCS Chemistry2020，2，3： | 1 |
| 3 | Effects of colostrums feeding on intestinal development in newborn pigs显示文摘 | Wang Tian Xu Ruojun | | 0，，06： | 1 |
| 4 | Effects of colostrums feeding on intestinal development in newborn pigs 显示文摘 | Wang Tian Xu Ruojun | 1996 | Biology Neonate1996，70，6： | 1 |
| 5 | An open-label evaluation of safety and tolerability of coformulated bictegravir/emtricitabine/tenofovir alafenamide for post-exposure prophylaxis following potential exposure to human immunodeficiency virus-1显示文摘Background: Single-tablet regimen (STR) provides a convenient once-daily regimen for the prevention of human immunodeficiency virus (HIV) infection. Here, we investigated the safety and tolerability of coformulated bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) as a three-drug, STR for post-exposure prophylaxis (PEP) in Chinese individuals.Methods: This was a prospective, open-label, single-arm trial conducted in a sexually transmitted diseases and acquired immunodeficiency syndrome clinic of a tertiary hospital in Beijing, China. Adults requiring PEP were prescribed BIC/FTC/TAF one pill once a day for 28 days. Clinical and laboratory data were collected and analyzed at baseline, weeks 2, 4, 8, 12, and 24.Results: Of 112 participants enrolled in the study, 109 (97.3%) were male and the mean age was 30 ± 8 years. PEP completion was 96.4% (95% confidence interval: 91.1-99.0%). Two participants stopped PEP after 2 days because the source partner was identified as HIV uninfected. One participant was excluded due to hepatitis B virus infection according to the exclusion criteria. One discontinued due to the participant’s decision. No participant acquired HIV through week 24. Adherence was 98.9% (standard deviation [SD]: 3.3%) by self-reporting and 98.5% (SD: 3.5%) by pill count. Only five participants experienced mild clinical adverse events attributed to the study drug (including headache, diarrhea, and nausea) and four participants had elevated serum creatinine (grade 1).Conclusions: A once daily, STR of BIC/FTC/TAF used as PEP was safe and well-tolerated with a high rate of completion and adherence in Chinese. BIC/FTC/TAF may be a good option for PEP. | An Liu Ruolei Xin Hongwei Zhang Lili Dai Ruojun(Esther)Wu Xi Wang Aixin Li Wei Hua Jianwei Li Ying Shao Yue Gao Zhangli Wang Jiangzhu Ye Gulimila A bu dou re xi ti Zaicun Li Lijun Sun | 2022 | Chinese Medical Journal2022，135，22： | 0 |
| 6 | An optimized prime editing system for efficient modification of the pig genome显示文摘Prime editing(PE)is a recent gene editing technology that can mediate insertions or deletions and all twelve types of base-tobase conversions.However,its low efficiency hampers the application in creating novel breeds and biomedical models,especially in pigs and other important farm animals.Here,we demonstrate that the pig genome is editable using the PE system,but the editing efficiency was quite low as expected.Therefore,we aimed to enhance PE efficiency by modulating both exogenous PE tools and endogenous pathways in porcine embryonic fibroblasts(PEFs).First,we modified the peg RNA by extending the duplex length and mutating the fourth thymine in a continuous sequence of thymine bases to cytosine,which significantly enhanced PE efficiency by improving the expression of peg RNA and targeted cleavage.Then,we targeted SAMHD1,a deoxynucleoside triphosphate triphosphohydrolase(d NTPase)that impedes the reverse transcription process in retroviruses,and found that treatment with its inhibitor,cephalosporin C zinc salt(CPC),increased PE efficiency up to 29-fold(4-fold on average),presumably by improving the reverse transcription process of Moloney murine leukemia virus reverse transcriptase(M-MLV RT)in the PE system.Moreover,PE efficiency was obviously improved by treatment with a panel of histone deacetylase inhibitors(HDACis).Among the four HDACis tested,panobinostat was the most efficient,with an efficiency up to 122-fold(7-fold on average),partly due to the considerable HDACi-mediated increase in transgene expression.In addition,the synergistic use of the three strategies further enhanced PE efficiency in PEFs.Our study provides novel approaches for optimization of the PE system and broadens the application scope of PE in agriculture and biomedicine. | Yanan Qi Ying Zhang Shuangjie Tian Ruojun Zong Xinghui Yan Yu Wang Yanfang Wang Jianguo Zhao | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 7 | Expanding our understanding of marine viral diversity through metagenomic analyses of biofilms显示文摘Recent metagenomics surveys have provided insights into the marine virosphere.However,these surveys have focused solely on viruses in seawater,neglecting those associated with biofilms.By analyzing 1.75 terabases of biofilm metagenomic data,3974 viral sequences were identified from eight locations around the world.Over 90%of these viral sequences were not found in previously reported datasets.Comparisons between biofilm and seawater metagenomes identified viruses that are endemic to the biofilm niche.Analysis of viral sequences integrated within biofilm-derived microbial genomes revealed potential functional genes for trimeric autotransporter adhesin and polysaccharide metabolism,which may contribute to biofilm formation by the bacterial hosts.However,more than 70%of the genes could not be annotated.These findings show marine biofilms to be a reservoir of novel viruses and have enhanced our understanding of natural virus-bacteria ecosystems. | Wei Ding Ruojun Wang Zhicong Liang Rui Zhang Pei-Yuan Qian Weipeng Zhan | 2021 | Marine Life Science & Technology2021，3，3： | 0 |
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