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| 1 | Normoalbuminuric diabetic kidney disease显示文摘 | Chao Chen Chang Wang Chun Hu Yachun Han Li Zhao Xuejing Zhu Li Xiao Lin Sun | 2017 | Frontiers of Medicine2017，11，3： | 38 |
| 2 | Variance components and correlations of female fertility traits in Chinese Holstein population显示文摘Background: The objective of the present study was to estimate(co)variance components of female fertility traits in Chinese Holsteins, considering fertility traits in different parities as different traits. Data on 88,647 females with 215,632 records(parities) were collected during 2000 to 2014 from 32 herds in the Sanyuan Lvhe Dairy Cattle Center, Beijing, China. The analyzed female fertility traits included interval from calving to first insemination, interval from first to last insemination, days open, conception rate at first insemination, number of inseminations per conception and non-return rates within 56 days after first insemination.Results: The descriptive statistics showed that the average fertility of heifers was superior to that of cows. Moreover,the genetic correlations between the performances of a trait in heifers and in cows were all moderate to high but far from one, which suggested that the performances of a trait in heifers and cows should be considered as different but genetically correlated traits in genetic evaluations. On the other hand, genetic correlations between performances of a trait in different parities of cows were greater than 0.87, with only a few exceptions, but variances were not homogeneous across parities for some traits. The estimated heritabilities of female fertility traits were low; all were below 0.049(except for interval from calving to first insemination). Additionally, the heritabilities of the heifer interval traits were lower than those of the corresponding cow interval traits. Moreover, the heritabilities of the interval traits were higher than those of the threshold traits when measuring similar fertility functions. In general, estimated genetic correlations between traits were highly consistent with the biological categories of the female fertility traits.Conclusions: Interval from calving to first insemination, interval from first to last insemination and non-return rates within 56 days after first insemination are recommended to be included in the selection index of the Chinese Holstein population. The parameters estimated in the present study will facilitate the development of a genetic evaluation system for female fertility traits to improve the reproduction efficiency of Chinese Holsteins. | Aoxing Liu Mogens Sandø Lund Yachun Wang Gang Guo Ganghui Dong Per Madsen Guosheng Su | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 6 |
| 3 | Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle显示文摘Background: Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count(SCC) and somatic cell score(SCS), serum cytokines such as interleukin-17(IL-17) and interleukin-4(IL-4) may also be potential indicators for bovine mastitis. The present study was designed to investigate the effects of single nucleotide polymorphisms(SNPs) in bovine IL-17 F and IL-17 A genes on SCC, SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle, and to compare the m RNA expression variations of the cows with different genotypes.Results: A total of 464 lactating cows(337 Holstein and 127 Inner-Mongolia Sanhe cattle) were screened for SNPs identification and the data were analyzed using fixed effects of herd, parity, season and year of calving by general linear model procedure. The results revealed that SNP g.24392436 C > T in IL-17 F and SNP g.24345410 A > G in IL-17 A showed significant effects on SCC and IL-4 in Holstein(n = 337) and on IL-17 and IL-4 in Sanhe cattle(n = 127). The homozygous GG genotype of SNP g.24345410 A > G had significantly higher m RNA expression compared with the heterozygous AG genotype.Conclusions: The results indicate that IL-17 F and IL-17 A could be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle. | Tahir Usman Yachun Wang Chao Liu Yanghua He Xiao Wang Yichun Dong Hongjun Wu Airong Liu Ying Yu | 2017 | Journal of Animal Science and Biotechnology2017，8，2： | 4 |
| 4 | GLOBAL STABILITY OF SOLUTIONS WITH DISCONTINUOUS INITIAL DATA CONTAINING VACUUM STATES FOR THE RELATIVISTIC EULER EQUATIONS显示文摘The global stability of Lipschitz continuous solutions with discontinuous initial data for the relativistic Euler equations is established in a broad class of entropy solutions in L∞ containing vacuum states. As a corollary, the uniqueness of Lipschitz solutions with discontinuous initial data is obtained in the broad class of entropy solutions in L∞. | LI YACHUN WANG LIBO | 2005 | Chinese Annals of Mathematics,Series B2005，26，4： | 4 |
| 5 | A novel method for rapid and reliable detection of complex vertebral malformation and bovine leukocyte adhesion deficiency in Holstein cattle显示文摘Background: Complex vertebral malformation (CVM) and bovine leukocyte adhesion deficiency (BLAD) are two autosomal recessive lethal genetic defects frequently occurring in Holstein cattle, identifiable by single nucleotide polymorphisms. The objective of this study is to develop a rapid and reliable genotyping assay to screen the active Holstein sires and determine the carrier frequency of CVM and BLAD in Chinese dairy cattle population. Results: We developed real-time PCR-based assays for discrimination of wild-type and defective alleles, so that carriers can be detected. Only one step was required after the DNA extraction from the sample and time consumption was about 2 hours. A total of 587 Chinese Holstein bulls were assayed, and fifty-six CVM-carriers and eight BLAD-carriers were identified, corresponding to heterozygote carrier frequencies of 9.54% and 1.36%, respectively. The pedigree analysis showed that most of the carriers could be traced back to the common ancestry, Osborndale Ivanhoe for BLAD and Pennstate Ivanhoe Star for CVM. Conclusions: These results demonstrate that real-time PCR is a simple, rapid and reliable assay for BLAD and CVM defective allele detection. The high frequency of the CVM allele suggests that implementing a routine testing system is necessary to gradually eradicate the deleterious gene from the Chinese Holstein population. | Yi Zhang Xuehua Fan Dongxiao Sun Yachun Wang Ying Yu Yan Xie Shengli Zhang Yuan Zhang | 2012 | Journal of Animal Science and Biotechnology2012，3，3： | 3 |
| 6 | Global Entropy Solutions of the Cauchy Problem for Nonhomogeneous Relativistic Euler System显示文摘我们分析 2 ×为在特殊相关性的完美的液体的 2 个非同类的相对论的 Euler 方程。我们在更低的顺序来源条款强加适当条件;在这些条件下面建立 Cauchy 问题的全球熵答案的存在。 | Yachun LI Anjiao WANG | 2006 | Chinese Annals of Mathematics,Series B2006，27，5： | 3 |
| 7 | Identification of novel molecular markers of mastitis caused by Staphylococcus aureus using gene expression profiling in two consecutive generations of Chinese Holstein dairy cattle显示文摘Background: Mastitis in dairy cows caused by Staphylococcus aureus is a major problem hindering economic growth in dairy farms worldwide. It is difficult to prevent or eliminate due to its asymptomatic nature and long persistence of infection. Although transcriptomic responses of bovine mammary gland cells to pathogens that cause mastitis have been studied, the common responses of peripheral blood leukocytes to S. aureus infection across two consecutive generations of dairy cattle have not been investigated.Methods: In the current study, RNA-Seq was used to profile the transcriptomes of peripheral blood leukocytes sampled from S. aureus-infected mothers and their S. aureus-infected daughters, and also healthy non-infected mothers and their healthy daughters. Differential gene expression was evaluated as follows: 1) S. aureus-infected cows versus healthy non-infected cows(S vs. H, which include all the mothers and daughters), 2) S. aureus-infected mothers versus healthy non-infected mothers(SM vs. HM), and 3) S. aureus-infected daughters versus healthy noninfected daughters(SMD vs. HMD).Results: Analysis of all identified expressed genes in the four groups(SM, SMD, HM, and HMD) showed that EPOR,IL9, IFNL3, CCL26, IL26 were exclusively expressed in both the HM and HMD groups, and that they were significantly(P < 0.05) enriched for the cytokine-cytokine receptor interaction pathway. A total of 17, 13 and 10 differentially expressed genes(DEGs)(FDR Padj. < 0.1 and |FC| > 1.2) were detected in the three comparisons, respectively. DEGs with P < 0.05 and |FC| > 2 were used for functional enrichment analyses. For the S vs. H comparison, DEGs detected included CCL20, IL13 and MMP3, which are associated with the IL-17 signaling pathway. In the SM vs. HM and SMD vs. HMD comparisons, five(BLA-DQB, C1 R, C2, FCGR1 A, and KRT10) and six(BLA-DQB, C3 AR1, CFI, FCAR, FCGR3 A, and LOC10498484) genes, respectively, were involved in the S. aureus infection pathway.Conclusions: Our study provides insights into the transcriptomic responses of bovine peripheral blood leukocytes across two generations of cattle naturally infected with S. aureus. The genes highlighted in this study could serve as expression biomarkers for mastitis and may also contain sequence variation that can be used for genetic improvement of dairy cattle for resilience to mastitis. | Di Wang Lei Liu Serafino M.A.Augustino Tao Duan Thomas J.Hall David E.MacHugh Jinhuan Dou Yi Zhang Yachun Wang Ying Yu | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 2 |
| 8 | Simulation study on the efficiencies of MOET nucleus breeding schemes applying marker assisted selection in dairy cattle显示文摘Advantages of breeding schemes using genetic marker information and/or multiple ovulation and embryo transfer(MOET) technology over the traditional approach were extensively evaluated through simulation.Milk yield was the trait of interest and QTL was the genetic marker utilized.Eight dairy cattle breeding scenarios were considered,i.e.,traditional progeny testing breeding scheme(denoted as STANPT),GASPT scheme including a pre-selection of young bulls entering progeny testing based on their own QTL information,MOETPT scheme using MOET technology to generate young bulls and a selection of young bulls limited within the full-sib family,GAMOPT scheme adopting both QTL pre-selection and MOET technology,COMBPT scheme using a mixed linear model which considered QTL genotype instead of the BLUP model in GAMOPT,and three non-progeny testing schemes,i.e.the MOET,GAMO and COMB schemes,corresponding to MOETPT,GAMOPT and COMBPT with progeny testing being part of the system.Animals were selected based on their breeding value which was estimated under an animal model framework.Sequential selection over 17 years was performed in the simulations and 30 replicates were designed for each scenario.The influences of using QTL information and MOET technology on favorable QTL allele frequency,true breeding values,polygenetic breeding values and the accumulated genetic superiority were extensively evaluated,for five different populations including active sires,lactating cows,bull dams,bull sires,and young bulls.The results showed that the combined schemes significantly outperformed other approaches wherein accumulated true breeding value progressed.The difference between schemes exclusively using QTL information or MOET technology was not significant.The STANPT scheme was the least efficient among the 8 schemes.The schemes using MOET technology had a higher polygenetic response than others in the 17th year.The increases of frequency of the favorable QTL allele varied more greatly across the 3 male groups than in the lactating cows group.The accumulated genetic superiorities of the GASPT scheme,MOETPT scheme,GAMOPT scheme,COMBPT scheme,MOET scheme,GAMO scheme and COMB scheme over the STANPT scheme were 8.42%,3.59%,14.58%,18.54%,4.12%,14.12%,16.50% in active sires and 2.70%,5.00%,11.05%,12.78%,7.51%,17.12%,25.38% in lactating cows. | LUO WeiZhen,WANG YaChun & ZHANG Yuan Department of Animal Genetics & Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100094,China | 2009 | Science China(Life Sciences)2009，52，3： | 2 |
| 9 | Understanding the Mechanical Properties of Vanadium Carbides: Nano-indentation Measurement and First-principles Calculations显示文摘 | Wu Lailei Yao Tiankai Wang Yachun | 2013 | Journal of Alloys and Compounds2013，548，1： | 1 |
| 10 | Genomic and transcriptomic analyses enable the identification of important genes associated with subcutaneous fat deposition in Holstein cows显示文摘Subcutaneous fat deposition has many important roles in dairy cattle,including immunological defense and mechanical protection.The main objectives of this study are to identify key candidate genes regulating subcutaneous fat deposition in high-producing dairy cows by integrating genomic and transcriptomic datasets.A total of 1654 genotyped Holstein cows are used to perform a genome-wide association study(GWAS)aiming to identify genes associated with subcutaneous fat deposition.Subsequently,weighted gene co-expression network analyses(WGCNA)are conducted based on RNA-sequencing data of 34 cows and cow yield deviations of subcutaneous fat deposition.Lastly,differentially expressed(DE)m RNA,lnc RNA,and differentially alternative splicing genes are obtained for 12 Holstein cows with extreme and divergent phenotypes for subcutaneous fat deposition.Forty-six protein-coding genes are identified as candidate genes regulating subcutaneous fat deposition in Holstein cattle based on GWAS.Eleven overlapping genes are identified based on the analyses of DE genes and WGCNA.Furthermore,the candidate genes identified based on GWAS,WGCNA,and analyses of DE genes are significantly enriched for pathways involved in metabolism,oxidative phosphorylation,thermogenesis,fatty acid degradation,and glycolysis/gluconeogenesis pathways.Integrating all findings,the NID2,STARD3,UFC1,DEDD,PPP1R1B,and USP21 genes are considered to be the most important candidate genes influencing subcutaneous fat deposition traits in Holstein cows.This study provides novel insights into the regulation mechanism underlying fat deposition in high-producing dairy cows,which will be useful when designing management and breeding strategies. | Hailiang Zhang Siyuan Mia Luiz F.Brito Lirong Hu Lei Wang Longgang Ma Qing Xu Gang Guo Ying Yu Yachun Wang | 2023 | Journal of Genetics and Genomics2023，50，6： | 1 |
| 11 | Adjuvant chemo-radiotherapy in the 'sandwich' method for high risk endometrial cancer-a review of literature显示文摘 | Yachun Bie Zhenyu Zhang Xiaolan Wang | 2015 | BMC Women''s Health2015，15，： | 1 |
| 12 | Association of bovine CD4 and STAT5b single nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins显示文摘 | Yanghua He Qin Chu Peipei Ma Yachun Wang Qin Zhang Dongxiao Sun Yi Zhang Ying Yu Yuan Zhang | 2011 | Journal of Dairy Research2011，，2： | 1 |
| 13 | Analysis on DNA Methylation of Various Tissues in Chicken显示文摘 | Qing Xu Yuan Zhang Dongxiao Sun Yachun Wang Ying Yu | 2007 | Animal Biotechnology2007，，4： | 1 |
| 14 | Crude oil mobility and its controlling factors in tight sand reservoirs in northern Songliao Basin, East China显示文摘Taking tight oil in Gaotaizi and Fuyu oil layers of the Upper Cretaceous Qingshankou Formation in northern Songliao Basin as an example, based on analyses of nuclear magnetic resonance and high pressure mercury injection, experiment methods of supercritical carbon dioxide displacement and extraction are firstly employed to quantify crude oil mobility in tight sand reservoirs with different lithologies and oil contents. The results show that, under the conditions of simulating the Cretaceous Qingshankou Formation in the northern Songliao Basin at a temperature of 76-89 °C and a pressure of 35-42 MPa, the lower limit of the porosity of the movable oil is4.4%, and the lower limit of the permeability is 0.015′10-3 mm2. The lower limit of the average pore throat radius is 21 nm. On this basis,a classification standard for three types of tight sand reservoirs is proposed. Type I reservoirs are characterized by the movable fluid saturation larger than 40%, the movable oil ratio(ratio of movable oil to total oil) greater than 30% and the starting pressure gradient in the range of 0.3-0.6 MPa/m; Type II reservoirs are characterized by the movable fluid saturation in the range of 10%–40%, the movable oil ratio in the range of 5%–30% and the starting pressure gradient in the range of 0.6–1.0 MPa/m; Type III reservoirs are characterized by the movable fluid saturation less than 10% in general, the movable oil ratio less than 5%, and the starting pressure gradient greater than1.0 MPa/m. The fluid mobility in tight sand reservoirs is mainly affected by diagenesis and sedimentary environment. Reservoirs with depth lower than 2000 m are dominated by type I reservoir, whereas those with greater depth are dominated by type I and II reservoirs.Reservoirs in inner delta-front facies are dominated by type I reservoir, whereas those in outer delta-front facies and shore-shallow lacustrine facies are dominated by type II and III reservoirs. | FENG Jun ZHANG Bowei FENG Zihui WANG Yachun ZHANG Juhe FU Xiaofei SUN Yonghe HUO Qiuli SHAO Hongmei ZENG Huasen QU Bin CHI Huanyuan | 2019 | Petroleum Exploration and Development2019，46，2： | 1 |
| 15 | Applications of the Kinetic Formulation for Scalar Conservation Laws with a Zero-Flux Type Boundary Condition显示文摘The authors are concerned with a zero-flux type initial boundary value problem for scalar conservation laws.Firstly,a kinetic formulation of entropy solutions is established.Secondly,by using the kinetic formulation and kinetic techniques,the uniqueness of entropy solutions is obtained.Finally,the parabolic approximation is studied and an error estimate of order η 1/3 between the entropy solution and the viscous approximate solutions is established by using kinetic techniques,where η is the size of artificial viscosity. | Zhigang WANG Yachun LI | 2012 | Chinese Annals of Mathematics,Series B2012，33，3： | 0 |
| 16 | Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells显示文摘Background:Heat stress is known to affect follicular dynamics,oocyte maturation,and fertilization by impairing steroidogenic ability and viability of bovine granulosa cell(bGCs).The present study explored the physiological and molecular response of bGCs to different heat stress intensities in-vitro.We exposed the primary bGCs to heat stress(HS)at 39℃,40℃ and 41℃ along with control samples(38℃)for 2 h.To evaluate the impact of heat stress on bGCs,several in vitro cellular parameters including cell apoptosis,intracellular reactive oxygen species(ROS)accumulation and HSP70 kinetics were assessed by flow cytometry,florescence microscopy and western blot,respectively.Furthermore,the ELISA was performed to confirm the 17β-estradiol(E2)and progesterone(P4)levels.In addition,the RNA sequencing(RNA-Seq)method was used to get the molecular based response of bGCs to different heat treatments.Results:Our findings revealed that the HS significantly decreased the cell viability,E2 and P4 levels in bGCs,whereas,increased the cellular apoptosis and ROS.Moreover,the RNA-Seq experiments showed that all the treatments(39℃,40℃ and 41℃)significantly regulated many differentially expressed genes(DEGs)i.e.BCL2L1,STAR,CYP11A1,CASP3,SOD2,HSPA13,and MAPK8IP1 and pathways associated with heat stress,apoptosis,steroidogenesis,and oxidative stress.Conclusively,our data demonstrated that the impact of 40°C treatment was comparatively detrimental for cell viability,apoptosis and ROS accumulation.Notably,a similar trend of gene expression was reported by RT-qPCR for RNA-seq data.Conclusions:Our study presented a worthy strategy for the first time to characterize the cellular and transcriptomic adaptation of bGCs to heat stress(39,40 and 41°C)in-vitro.The results infer that these genes and pathways reported in present study could be useful candidates/indicators for heat stress research in dairy cattle.Moreover,the established model of bGCs to heat stress in the current study provides an appropriate platform to understand the mechanism of how heat-stressed bGCs can affect the quality of oocytes and developing embryo. | Adnan Khan Jinhuan Dou Yachun Wang Xiaolong Jiang Muhammad Zahoor Khan Hanpeng Luo Tahir Usman Huabin Zhu | 2020 | Journal of Animal Science and Biotechnology2020，11，2： | 0 |
| 17 | Formation Pressure Evaluation Method for Single Well显示文摘The paper deals with the methods of formation pressure evaluation for a single well by using the very common accepted parameters, such as drilling exponent , and flowline temperature , etc. which is part of compiling the end well report. | WANG Yachun( Petroleum University, Beijing 100082 P. R.China)LIU Tao(Geologging Company of Daqing Oiffield, Heilongjiang 163411, P. R.China) | 2002 | Global Geology2002，7，1： | 0 |
| 18 | Weighted single-step GWAS and RNA sequencing reveals key candidate genes associated with physiological indicators of heat stress in Holstein cattle显示文摘Background: The study of molecular processes regulating heat stress response in dairy cattle is paramount for developing mitigation strategies to improve heat tolerance and animal welfare. Therefore, we aimed to identify quantitative trait loci(QTL) regions associated with three physiological indicators of heat stress response in Holstein cattle, including rectal temperature(RT), respiration rate score(RS), and drooling score(DS). We estimated genetic parameters for all three traits. Subsequently, a weighted single-step genome-wide association study(WssGWAS) was performed based on 3200 genotypes, 151,486 phenotypic records, and 38,101 animals in the pedigree file. The candidate genes located within the identified QTL regions were further investigated through RNA sequencing(RNA-seq) analyses of blood samples for four cows collected in April(non-heat stress group) and four cows collected in July(heat stress group).Results: The heritability estimates for RT, RS, and DS were 0.06, 0.04, and 0.03, respectively. Fourteen, 19, and 20 genomic regions explained 2.94%, 3.74%, and 4.01% of the total additive genetic variance of RT, RS, and DS, respectively. Most of these genomic regions are located in the Bos taurus autosome(BTA) BTA3, BTA6, BTA8, BTA12, BTA14, BTA21, and BTA24. No genomic regions overlapped between the three indicators of heat stress, indicating the polygenic nature of heat tolerance and the complementary mechanisms involved in heat stress response. For the RNA-seq analyses, 2627 genes were significantly upregulated and 369 downregulated in the heat stress group in comparison to the control group. When integrating the WssGWAS, RNA-seq results, and existing literature, the key candidate genes associated with physiological indicators of heat stress in Holstein cattle are: PMAIP1, SBK1, TMEM33, GATB, CHORDC1, RTN4IP1, and BTBD7.Conclusions: Physiological indicators of heat stress are heritable and can be improved through direct selection. Fifty-three QTL regions associated with heat stress indicators confirm the polygenic nature and complex genetic determinism of heat tolerance in dairy cattle. The identified candidate genes will contribute for optimizing genomic evaluation models by assigning higher weights to genetic markers located in these regions as well as to the design of SNP panels containing polymorphisms located within these candidate genes. | Hanpeng Luo Lirong Hu Luiz F.Brito Jinhuan Dou Abdul Sammad Yao Chang Longgang Ma Gang Guo Lin Liu Liwei Zhai Qing Xu Yachun Wang | 2022 | Journal of Animal Science and Biotechnology2022，13，6： | 0 |
| 19 | Cs_(3)Bi_(2)l_(9)-hydroxyapatite composite waste forms for cesium and iodine immobilization显示文摘Perovskite-based ceramic composites were developed as potential waste form materials for immobilizing cesium(Cs)and iodine(I)with high waste loadings and chemical durability.The perovskite Cs_(3)Bi_(2)I_(9)has high Cs(22 wt%)and I(58 wt%)content,and thus can be used as a potential host phase to immobilize these critical radionuclides.In this work,the perovskite Cs_(3)Bi_(2)I_(9)phase was synthesized by a cost effective solution-based approach,and was embedded into a highly durable hydroxyapatite matrix by spark plasma sintering to form dense ceramic composite waste forms.The chemical durabilities of the monolithic Cs_(3)Bi_(2)I_(9)and Cs_(3)Bi_(2)I_(9)-hydroxyapatite composite pellets were investigated by static and semi-dynamic leaching tests,respectively.Cs and I are incongruently released from the matrix for both pure Cs_(3)Bi_(2)I_(9)and composite structures.The normalized Cs release rate is faster than that of I,which can be explained by the difference in the strengths between Cs-I and Bi-I bonds as well as the formation of insoluble micrometer-sized BiOI precipitates.The activation energies of elemental releases based on dissolution and diffusion-controlled mechanisms are determined with significantly higher energy barriers for dissolution from the composite versus that of the monolithic Cs_(3)Bi_(2)I_(9).The ceramic-based composite waste forms exhibit excellent chemical durabilities and waste loadings,commensurate with the state-of-the-art glass-bonded perovskite composites for I and Cs immobilization. | Kun YANG Yachun WANG Junhua SHEN Spencer M.SCOTT Brian J.RILEY John D.VIENNA Jie LIAN | 2022 | Journal of Advanced Ceramics2022，11，5： | 0 |
| 20 | Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway显示文摘WRKY transcription factors,transcriptional regulators unique to plants,play an important role in defense response to pathogen infection.However,the resistance mechanisms of WRKY genes in sugarcane remain unclear.In the present study,gene ontology(GO)enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense response.We identified gene ScWRKY4,a classⅡc member of the WRKY gene family,in sugarcane cultivar ROC22.This gene was induced by salicylic acid(SA)and methyl jasmonate(MeJA)stress.Interestingly,expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium scitamineum.Moreover,stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani var.coeruleum and caused down-regulated expression of immune marker-related genes.Transcriptome analysis indicated suppressed expression of most JAZ genes in the signal transduction pathway.ScWRKY4 interacted with ScJAZ13 to repress its expression.We thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance,most likely through the JA signaling pathway.The present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation. | Dongjiao Wang Wei Wang Shoujian Zang Liqian Qin Yanlan Liang Peixia Lin Yachun Su Youxiong Que | 2024 | The Crop Journal2024，12，1： | 0 |
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