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| 1 | Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis显示文摘精子发生在双 spermatogonial 干细胞(SSC ) 哪个生产 haploid 精子期间是一个区别过程。这个高度专业化的过程精确在 transcriptional, posttranscriptional,和翻译层次被控制。这里,我们报导那 N 6-methyladenosine (m 6 一) ,调整基因表示的一个 epitranscriptomic 标记,在精子发生期间起必要作用。我们现在的全面 m 6 从五个发展阶段的老鼠 spermatogenic 房间的 mRNA methylomes:无差别的 spermatogonia,类型 A 1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes,和圆 spermatids。m 6 有 Vasa-Cre 的 RNA methyltransferase Mettl3 或 Mettl14 引起 m 6 SSC 的 A 和弄空。m 6 为 SSC 增长 / 区别被要求的抄本的一个弄空 dysregulates 翻译。在有 Stra8-GFPCre 的先进细菌房间的 Mettl3 和 Mettl14 的联合删除破坏精子形式,而有在先进细菌房间的 Mettl3 或 Mettl14 的单个删除的老鼠显示出正常精子发生。从双异种老鼠展览的 spermatids 损害了为精子形式是必要的 haploid 特定的基因的翻译。这研究加亮 mRNA m 6 在 germline 开发的修正,潜在地保证在精子发生的不同阶段协调了翻译。 | Zhen Lin Phillip J Hsu Xudong Xing Jianhuo Fang Zhike Lu Qin Zou Ke-Jia Zhang Xiao Zhang Yuchuan Zhou Teng Zhang Youcheng Zhang Wanlu Song Guifang Jia Xuerui Yang Chuan He Ming-Han Tong | 2017 | Cell Research2017，27，10： | 61 |
| 2 | A New Method of Wind Turbine Bearing Fault Diagnosis Based on Multi-Masking Empirical Mode Decomposition and Fuzzy C-Means Clustering显示文摘Based on Multi-Masking Empirical Mode Decomposition (MMEMD) and fuzzy c-means (FCM) clustering, a new method of wind turbine bearing fault diagnosis FCM-MMEMD is proposed, which can determine the fault accurately and timely. First, FCM clustering is employed to classify the data into different clusters, which helps to estimate whether there is a fault and how many fault types there are. If fault signals exist, the fault vibration signals are then demodulated and decomposed into different frequency bands by MMEMD in order to be analyzed further. In order to overcome the mode mixing defect of empirical mode decomposition (EMD), a novel method called MMEMD is proposed. It is an improvement to masking empirical mode decomposition (MEMD). By adding multi-masking signals to the signals to be decomposed in different levels, it can restrain low-frequency components from mixing in highfrequency components effectively in the sifting process and then suppress the mode mixing. It has the advantages of easy implementation and strong ability of suppressing modal mixing. The fault type is determined by Hilbert envelope finally. The results of simulation signal decomposition showed the high performance of MMEMD. Experiments of bearing fault diagnosis in wind turbine bearing fault diagnosis proved the validity and high accuracy of the new method. | Yongtao Hu Shuqing Zhang Anqi Jiang Liguo Zhang Wanlu Jiang Junfeng Li | 2019 | Chinese Journal of Mechanical Engineering2019，32，3： | 6 |
| 3 | Classification of four distinct osteoarthritis subtypes with a knee joint tissue transcriptome atlas显示文摘The limited molecular classifications and disease signatures of osteoarthritis(OA)impede the development of prediagnosis and targeted therapeutics for OA patients.To classify and understand the subtypes of OA,we collected three types of tissue including cartilage,subchondral bone,and synovium from multiple clinical centers and constructed an extensive transcriptome atlas of OA patients.By applying unsupervised clustering analysis to the cartilage transcriptome,OA patients were classified into four subtypes with distinct molecular signatures:a glycosaminoglycan metabolic disorder subtype(C1),a collagen metabolic disorder subtype(C2),an activated sensory neuron subtype(C3),and an inflammation subtype(C4).Through ligand-receptor crosstalk analysis of the three knee tissue types,we linked molecular functions with the clinical symptoms of different OA subtypes.For example,the Gene Ontology functional term of vasculature development was enriched in the subchondral bone-cartilage crosstalk of C2 and the cartilage-subchondral bone crosstalk of C4,which might lead to severe osteophytes in C2 patients and apparent joint space narrowing in C4 patients.Based on the marker genes of the four OA subtypes identified in this study,we modeled OA subtypes with two independent published RNA-seq datasets through random forest classification.The findings of this work contradicted traditional OA diagnosis by medical imaging and revealed distinct molecular subtypes in knee OA patients,which may allow for precise diagnosis and treatment of OA. | Chunhui Yuan Zongyou Pan Kun Zhao Jun Li Zixuan Sheng Xudong Yao Hua Liu Xiaolei Zhang Yang Yang Dongsheng Yu Yu Zhang Yuzi Xu Zhi-Yong Zhang Tianlong Huang Wanlu Liu Hongwei Ouyang | 2020 | Bone Research2020，8，4： | 4 |
| 4 | Effect of Oxygen Concentration and Annealing Theatment on the Optical Properties of the Transparent Conductive CdIn_2O_4 Thin Films显示文摘Transparent conductive cadmium indium oxide films (CdIn2O4) were prepared by r.f. reactive sputtering from Cd-In alloy targets under an Ar-O2 atmosphere. Electrical conductivity of the order of 105Ω-1.m-1 and the optical transmission as high as 94% are easily attained by postdeposition annealing treatment. The effects of oxygen concentration in the reactive gas mixture and post-deposition annealing treatment on the optical transmittance as well as optical parameters, such as refractive index (n), extinction coefficient (k), real part (ε’) and imaginary part (ε') of the dielectric constant, were studied in the visible and near-infrared region. The highfrequency dielectric constant ε∞ the plasma frequency ωP, and the conduction band effective mass mc of different samples were also | Bin WU Changyong CHEN and Shibin ZHANG(Physics Dept., Lanzhou University, Lanzhou 730000, China)Wanlu WANG and Kejun LIAO(Applied Physics Dept., Chongqing University, Chongqing 400044, China) | 1998 | Journal of Materials Science & Technology1998，14，2： | 4 |
| 5 | Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation显示文摘Fundamental processes such as ribosomal RNA synthesis and chromatin remodeling take place in the nucleolus,which is hyperactive in fast-proliferating cells.The sophisticated regulatory mechanism underlying the dynamic nucleolar structure and functions is yet to be fully explored.The present study uncovers the mutual functional dependency between a previously uncharacterized human long non-coding RNA,which we renamed LETN,and a key nucleolar protein,NPM1.Specifically,being upregulated in multiple types of cancer,LETN resides in the nucleolus via direct binding with NPM1.LETN plays a critical role in facilitating the formation of NPM1 pentamers,which are essential building blocks of the nucleolar granular component and control the nucleolar functions.Repression of LETN or NPM1 led to similar and profound changes of the nucleolar morphology and arrest of the nucleolar functions,which led to proliferation inhibition of human cancer cells and neural progenitor cells.Interestingly,this inter-dependency between LETN and NPM1 is associated with the evolutionarily new variations of NPM1 and the coincidental emergence of LETN in higher primates.We propose that this human-specific protein–lncRNA axis renders an additional yet critical layer of regulation with high physiological relevance in both cancerous and normal developmental processes that require hyperactive nucleoli. | Xianteng Wang Xiaolin Hu Wanlu Song Hui Xu Zhengtao Xiao Rongyao Huang Qingran Bai Fan Zhang Yongzhen Chen Yu Liu Jianhuo Fang Xin Li Qin Shen Haitao Zhao Xuerui Yang | 2021 | Cell Research2021，31，6： | 3 |
| 6 | Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins显示文摘Piericidins are a large family of bacterialα-pyridone antibiotics with antitumor activities such as their anti-renal carcinoma activity exhibited recently in nude mice.The backbones of piericidins are derived fromβ,δ-diketo carboxylic acids,which are offloaded from a modular polyketide synthase(PKS)and putatively undergo a carbonyl amidation beforeα-pyridone ring formation.The tailoring modifications to theα-pyridone structure mainly include the verified hydroxylation and O-methylation of the C-4′position and an unidentified C-5′O-methylation.Here,we describe a piericidin producer,terrestrial Streptomyces conglobatus,which contains a piericidin biosynthetic gene cluster in two different loci.Deletion of the amidotransferase gene pieD resulted in the accumulation of two fatty acids that should be degraded from the nascent carboxylic acid released by the PKS,supporting the carbonyl amidation function of PieD duringα-pyridone ring formation.Deletion of the O-methyltransferase gene pieB1 led to the production of three piericidin analogues lacking C-5′O-methylation,therefore confirming that PieB1 specifically catalyses the tailoring modification.Moreover,bioactivity analysis of the mutant-derived products provided clues regarding the structure-function relationship for antitumor activity.The work addresses two previously unidentified steps involved in pyridyl pharmacophore formation during piericidin biosynthesis,facilitating the rational bioengineering of the biosynthetic pathway towards valuable antitumor agents. | Wanlu Li Wenyu Zhang Yijia Cheng Yaoyao Shen Jianzhao Qi Hou-Wen Lin Yongjun Zhou | 2022 | Synthetic and Systems Biotechnology2022，7，3： | 2 |
| 7 | Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx^(−/−)mice显示文摘Tendon heterotopic ossification(HO)is characterized by bone formation inside tendon tissue,which severely debilitates people in their daily life.Current therapies fail to promote functional tissue repair largely due to our limited understanding of HO pathogenesis.Here,we investigate the pathological mechanism and propose a potential treatment method for HO.Immunofluorescence assays showed that the Mohawk(MKX)expression level was decreased in human tendon HO tissue,coinciding with spontaneous HO and the upregulated expression of osteochondrogenic and angiogenic genes in the tendons of Mkx^(−/−)mice.Single-cell RNA sequencing analyses of wild-type and Mkx^(−/−)tendons identified three cell types and revealed the excessive activation of osteochondrogenic genes during the tenogenesis of Mkx^(−/−)tendon cells.Single-cell analysis revealed that the gene expression program of angiogenesis,which is strongly associated with bone formation,was activated in all cell types during HO.Moreover,inhibition of angiogenesis by the small-molecule inhibitor BIBF1120 attenuated bone formation and angiogenesis in the Achilles tendons of both Mkx mutant mice and a rat traumatic model of HO.These findings provide new insights into the cellular mechanisms of tendon HO and highlight the inhibition of angiogenesis with BIBF1120 as a potential treatment strategy for HO. | Junxin Lin Yuwei Yang Wenyan Zhou Chao Dai Xiao Chen Yuanhao Xie Shan Han Huanhuan Liu Yejun Hu Chenqi Tang Varitsara Bunpetch Dandan Zhang Yishan Chen Xiaohui Zou Di Chen Wanlu Liu Hongwei Ouyang | 2022 | Bone Research2022，10，4： | 1 |
| 8 | Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052显示文摘 | Zhang Wanlu Liu Ziyong Liu Zhen | 2012 | Letters in Applied Microbiology2012，55，3： | 1 |
| 9 | Synthesis of hollow polyaniline nano-capsules and their supercapacitor application显示文摘 | Wanlu Yang Zan Gao Ningning Song Yunya Zhang Yingchao Yang Jun Wang | 2014 | Journal of Power Sources2014，，： | 1 |
| 10 | Quantum dots-hydrogel composites for biomedical applications显示文摘Quantum dots-hydrogel composites are promising new materials that have attracted extensive attention due to their incomparable biocompatibility and acceptable biodegradability, leading to enormous potential applications for various fields. This review summarizes the recent advances in quantum dots-hydrogel composites with a focus on synthesis methods, including hydrogel gelation in quantum dots(QDs) solution, embedding prepared QDs into hydrogels after gelation, forming QDs in situ within the preformed gel and cross-linking via QDs to form hydrogels. In particularly, biomedical applications as bioimaging,biosensing and drug delivery are also reviewed, followed by a discussion on the inherent challenges of design optimization, biocompatibility and bimodal applications and the prospect of the future development. These results will guide the development of quantum dots-hydrogel composites and provide critical insights to inspire researchers in future. | Wenjie Zhou Zhe Hu Jinxin Wei Hanqing Dai Yuanyuan Chen Siyu Liu Zhongtao Duan Fengxian Xie Wanlu Zhang Ruiqian Guo | 2022 | Chinese Chemical Letters2022，33，3： | 1 |
| 11 | Superwettable interface towards biodetection in confined space显示文摘The detection of biomarkers with both high sensitivity and specificity is crucial for the diagnosis and treatment of related diseases.However,many current detections employ ex-situ detection method and non-confined condition,thus have many problems,which may eventually lead to inaccurate detection results.Compared to detection in non-confined space,detection in confined space can better reflect the real in-vivo situation.Therefore,the construction of detection for target molecules in confined space has great significance for both theoretical research and practical application.To realize the detection of target molecules in confined space,the probes should accurately enter the confined space where the target molecules reside and interact with the interface.Thus,how to explore and utilize the properties of the interface(for example,bioinspired superwettability)has always been a hot and difficult topic in this field.Herein,the recent advances and our efforts in recent 10 years on detection of bio-target molecules in confined space with superwettable interface have been introduced from the perspective of the detection methods.The suitable and most widely employed detection methods for target molecules in confined spaces are introduced firstly.Then,recent progresses for related detections based on visual,optical,and electrochemical detection methods are presented successively.Finally,the perspective for detection in confined space is discussed for the future development of biochemical detection. | Zexu Pang Zhikang Cao Wanlu Li Wenxia Xu Yingying Zhang Qitao Zhou Jing Pan Fan Xia | 2024 | Nano Research2024，17，2： | 1 |
| 12 | Aqueous synthesis of 79%efficient AgInGaS/ZnS quantum dots for extremely high color rendering white light-emitting diodes显示文摘Over the past few decades,I-III-VI quantum dots(QDs)have attracted considerable attention due to their large Stokes shifts,good eco-friendliness,and wide tunable emissions.In this work,a facile microwave-assisted aqueous route using glutathione(GSH)and citric acid(CA)as dual stabilizers is introduced to synthesize AgInGaS/ZnS(AIGS/ZnS)core/shell QDs.An exceptional photoluminescence quantum yield(PL QY)as high as 79%is successfully achieved,boosting the best performance of water-dispersible AIGS QDs.By varying the Ag/Ga ratio,the PL peak wavelength can be tuned from 547 to 616 nm,and luminescent hydrogel films with different colors are obtained by embedding AIGS/ZnS QDs in polyacrylamide/poly-vinyl alcohol(PAAm/PVA)hydrogels.The white light-emitting diode(WLED)with a high color rendering index(CRI)of 92.1(R_(9)=92.0)and a correlated color temperature(CCT)of 3022 K is fabricated using QDs-PAAm/PVA hydrogel films in combination with a blue InGaN LED chip,indicating that the as-prepared QDs are competitive color-conversion materials for WLEDs. | Zhe Hu Hanxu Lu Wenjie Zhou Jinxin Wei Hanqing Dai b Hong Liu Zhiyong Xiong Fengxian Xie Wanlu Zhang Ruiqian Guo | 2023 | Journal of Materials Science & Technology2023，，3： | 1 |
| 13 | Synthesis of reduced graphene nanosheet/urchin-like manganese dioxide composite and high performance as supercapacitor electrode显示文摘 | Wanlu Yang Zan Gao Jun Wang Bin Wang Qi Liu Zhanshuang Li Tom Mann Piaoping Yang Milin Zhang Lianhe Liu | 2012 | Electrochimica Acta2012，，： | 1 |
| 14 | Periplasmic expression optimization of VEGFR2 D3 adopting response surface methodology: antiangiogenic activity study 显示文摘 | CAO Wanlu LI Halxin ZHANG Juan | 2013 | Pro- tein Expr Purif2013，90，2： | 1 |
| 15 | Comparative study on nutrient composition and antioxidant capacity of potato based on geographical and climatic factors显示文摘Potatoes are consumed in the human diet,but the environmental effects on the nutrient content of their tubers are not known.Five potato varieties from China(Chongqing,Dingxi,Qiqihar,Enshi,and Ulanqab)were selected to compare their nutritional components and antioxidant capacity and to explore the correlation between nutrition and local rainfall,humidity,temperature,and light time.The results indicated the Qiqihar cultivar presents significantly higher antioxidant capacity,and has the most elevated protein and soluble sugar contents.The total phenol(TPC)(325.07μg/g)and carotenoid contents(54.93μg/100g)of the Ulanqab cultivar were the highest.The Dingxi cultivar reveals the highest total flavonoids(TFC)(168.94μg/g),K,Mg,and P contents.The ascorbic acid content(26.09 mg/100g)was the highest in the Chongqing cultivar,and the contents of starch,Ca,total acid,and water were also prominent.The amino acid content(7.350%)in the Enshi cultivar is higher.It was found that soluble sugar was significantly correlated with humidity,temperature,and light time;carotenoids were affected considerably by rainfall;ascorbic acid has a strong correlation with temperature;TPC was moderately associated with humidity and light time.To summarize,tubers’nutrient composition and antioxidant capacity vary with geographical location and climate of origin.This study will help provide a theoretical basis for selecting geographical areas for potato planting and exploring the complex interactive factors between environment and vegetable attributes. | Bing Zhang Ayesha Murtaza Aamir Iqbal Jiao Zhang Tingting Bai Wanlu Ma Xiaoyun Xu Siyi Pan Wanfeng Hu | 2022 | Food Bioscience2022，46，2： | 1 |
| 16 | Fabrication of fibrous szaibelyite with hierarchical structure superhydrophobic coating on AZ31 magnesium alloy for corrosion protection显示文摘 | Rui Gao Qi Liu Jun Wang Xiaofei Zhang Wanlu Yang Jingyuan Liu Lianhe Liu | 2013 | Chemical Engineering Journal2013，，： | 1 |
| 17 | 3D-bioprinted cholangiocarcinoma-on-a-chip model for evaluating drug responses显示文摘Cholangiocarcinoma(CCA)is characterized by heterogeneous mutations and a refractory nature.Thus,the development of a model for effective drug screening is urgently needed.As the established therapeutic testing models for CCA are often ineffective,we fabricated an enabling three-dimensional(3D)-bioprinted CCA-on-a-chip model that to a good extent resembled the multicellular microenvironment and the anatomical microstructure of the hepato-vascular-biliary system to perform high-content antitumor drug screening.Specifically,cholangiocytes,hepatocytes,and vascular endotheliocytes were employed for 3D bioprinting of the models,allowing for a high degree of spatial and tube-like microstructural control.Interestingly,it was possible to observe CCA cells attached to the surfaces of the gelatin methacryloyl(GelMA)hydrogelembedded microchannels and overgrown in a thickening manner,generating bile duct stenosis,which was expected to be analogous to the in vivo configuration.Over 4000 differentially expressed genes were detected in the CCA cells in our 3D coculture model compared to the traditional two-dimensional(2D)monoculture.Further screening revealed that the CCA cells grown in the 3D traditional model were more sensitive to the antitumoral prodrug than those in the 2D monoculture due to drug biotransformation by the neighboring functional hepatocytes.This study provides proof-of-concept validation of our bioprinted CCA-on-a-chip as a promising drug screening model for CCA treatment and paves the way for potential personalized medicine strategies for CCA patients in the future. | Qiong Liu Luis SMille Cesar Villalobos Ingrid Anaya Matthias Vostatek Sili Yi Wanlu Li Junlong Liao Huanghui Wu Yongteng Song Lize Xiong Yu Shrike Zhang | 2023 | Bio-Design and Manufacturing2023，6，4： | 0 |
| 18 | AgInS_(2)/ZnS quantum dots for noninvasive cervical cancer screening with intracellular pH sensing using fluorescence lifetime imaging microscopy显示文摘Intracellular pH plays a critical role in biological functions,and abnormal pH values are related to various diseases.Here,we report on an intracellular pH sensor AgInS_(2)(AIS)/ZnS quantum dots(QDs)that show long fluorescence lifetimes of hundreds of nanoseconds and low toxicity.Fluorescence lifetime imaging microscopy(FLIM)combined with AIS/ZnS QDs is used for the imaging of live cells in different pH buffers and different cell lines.The FLIM images of AIS/ZnS QDs in live cells demonstrate different intracellular pH values in different regions,such as in lysosomes or cytoplasm.This method can also distinguish cancer cells from normal cells,and the fluorescence lifetime difference of the AIS/ZnS QDs between the two types of cells is 100±7 ns.Most importantly,the exfoliated cervical cells from 20 patients are investigated using FLIM combined with AIS/ZnS QDs.The lifetime difference value between the normal and cervical cancer(CC)groups is 115±9 ns,and the difference between the normal and the precancerous lesion group is 64±9 ns.For the first time,the noninvasive method has been used for cervical cancer screening,and it has shown great improvement in sensitivity compared with a clinical conventional cytology examination. | Wenhua Su Dan Yang Yulan Wang Yawei Kong Wanlu Zhang Jing Wang Yiyan Fei Ruiqian Guo Jiong Ma Lan Mi | 2022 | Nano Research2022，15，6： | 0 |
| 19 | Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase显示文摘The bacterial trialkyl-substituted aromatic polyketides are structurally featured with the unusual aromatic core in the middle of polyketide chain such as TM-123(1),veramycin A(2),NFAT-133(3)and benwamycin I(4),which were discovered from Streptomyces species and demonstrated with antidiabetic and immunosuppressant activities.Though the biosynthetic pathway of 1-3 was reported as a type I polyketide synthase(PKS),the PKS assembly line was interpreted inconsistently,and it remains a mystery how the compound 3 was generated.Herein,the PKS assembly logic of 1-4 was revised by site-mutagenetic analysis of the PKS dehydratase domains.Based on gene deletion and complementation,the putative P450 monooxygenase nftE1 and metallo-beta-lactamase(MBL)fold hydrolase nftF1 were verified as essential genes for the biosynthesis of 1-4.The absence of nftE1 led to abolishment of 1-4 and accumulation of new products(5-8).Structural elucidation reveals 5-8 as the non-aromatic analogs of 1,suggesting the NftE1-catalyzed aromatic core formation.Deletion of nftF1 resulted in disappearance of 3 and 4 with the compounds 1 and 2 unaffected.As a rare MBL-fold hydrolase from type I PKSs,NftF1 potentially generates the compound 3 through two strategies:catalyze premature chain-offloading as a trans-acting thioesterase or hydrolyze the lactone-bond of compound 1 as an esterase. | Ming Yang Wanlu Li Lin Zhou Xiao Lin Wenyu Zhang Yaoyao Shen Hai Deng Hou-wen Lin Yongjun Zhou | 2023 | Synthetic and Systems Biotechnology2023，8，3： | 0 |
| 20 | The US/China workshop: Regulation, standards, and innovation IV, organized by the Chinese Society for Biomaterials (CSBM) and the US Society for Biomaterials (SFB) in Minneapolis 2017显示文摘The 4th Joint Workshop organized by the Chinese Society for Biomaterials(CSBM)and the US Society for Biomaterials(SFB),the US/China Workshop:Regulation,Standards,and Innovation IV was successfully held in Minneapolis on April 5th,2017 during the SFB 2017 Annual Meeting.Following the first three annual workshops hosted in both the US(the 1st at Boston in 2013 and the 2nd at Denver in 2014)and China(the 3rd at Haikou in 2015),it is the first biennial workshop after a joint business meeting between CSBM and US SFB during the 10th World Biomaterials Congress in 2016 determined that future joint workshop will be hosted at each country every other year starting in 2017. | Wanlu Zhao Liisa Kuhn Arthur Coury Kai Zhang Xingdong Zhang | 2017 | Bioactive Materials2017，2，2： | 0 |
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