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| 1 | High-throughput phenotyping: Breaking through the bottleneck in future crop breeding显示文摘With the rapid development of genetic analysis techniques and crop population size,phenotyping has become the bottleneck restricting crop breeding.Breaking through this bottleneck will require phenomics,defined as the accurate,high-throughput acquisition and analysis of multi-dimensional phenotypes during crop growth at organism-wide levels,ranging from cells to organs,individual plants,plots,and fields.Here we offer an overview of crop phenomics research from technological and platform viewpoints at various scales,including microscopic,ground-based,and aerial phenotyping and phenotypic data analysis.We describe recent applications of high-throughput phenotyping platforms for abiotic/biotic stress and yield assessment.Finally,we discuss current challenges and offer perspectives on future phenomics research. | Peng Song Jinglu Wang Xinyu Guo Wanneng Yang Chunjiang Zhao | 2021 | The Crop Journal2021，9，3： | 10 |
| 2 | A deep learning-integrated micro-CT image analysis pipeline for quantifying rice lodging resistance-related traits显示文摘Lodging is a common problemin rice,reducing its yield andmechanical harvesting efficiency.Rice architecture is a key aspect of its domestication and a major factor that limits its high productivity.The ideal rice culm structure,includingmajor_axis_culm,minor axis_culm,andwall thickness_culm,is critical for improving lodging resistance.However,the traditionalmethod ofmeasuring rice culms is destructive,time consuming,and labor intensive.In this study,we used a high-throughput micro-CT-RGB imaging system and deep learning(SegNet)todevelopa high-throughputmicro-CTimageanalysis pipelinethatcanextract 24 riceculmmorphological traits and lodging resistance-related traits.When manual and automatic measurements were compared at themature stage,the mean absolute percentage errors for major_axis_culm,minor_axis_culm,andwall_thickness_culmin 104 indica rice accessionswere 6.03%,5.60%,and 9.85%,respectively,and the R^(2) valueswere 0.799,0.818,and 0.623.We also builtmodels of bending stress using culmtraits at the mature and tillering stages,and the R^(2) values were 0.722 and 0.544,respectively.The modeling results indicated that this method can quantify lodging resistance nondestructively,even at an early growth stage.In addition,we also evaluated the relationships of bending stress toshoot dryweight,culm density,and drought-related traits and found that plants with greater resistance to bending stress had slightly higher biomass,culm density,and culm area but poorer drought resistance.In conclusion,we developed a deep learning-integrated micro-CT image analysis pipeline to accurately quantify the phenotypic traits of rice culms in4.6 min per plant;this pipeline will assist in future high-throughput screening of large rice populations for lodging resistance. | Di Wu Dan Wu Hui Feng Lingfeng Duan Guoxing Dai Xiao Liu Kang Wang Peng Yang Guoxing Chen Alan P.Gay John H.Doonan Zhiyou Niu Lizhong Xiong Wanneng Yang | 2021 | Plant Communications2021，2，2： | 7 |
| 3 | Hierarchical red phosphorus incorporated TiO_(2) hollow sphere heterojunctions toward superior photocatalytic hydrogen production显示文摘Solar-to-hydrogen conversion through photocatalysis is a sustainable and promising strategy for storing solar energy.Recently,elemental red phosphorus(RP)with broad light absorption has been recognized as a potential candidate for photocatalytic hydrogen evolution,while challenges remain due to the rapid recombination of photogenerated carriers.In this work,RP modified TiO_(2)hollow spheres were designed and fabricated through the chemical vapor deposition method.The optimal hydrogen production rate reaching 215.5μmol/(g h)over TiO_(2)@RP heterostructure was obtained under simulated solar light irradiation.Experimental results evidenced that the hollow sphere structure and RP light absorber extended light absorption ability,and the heterostructure induced interfacial charge migration facilitated photoinduced charge separation,which benefited the photocatalytic hydrogen production performance. | Guiqing Huang Wanneng Ye Chunxiao Lv Denys S.Butenko Chen Yang Gaolian Zhang Ping Lu Yan Xu Shuchao Zhang Hongwei Wang Yukun Zhu Dongjiang Yang | 2022 | Journal of Materials Science & Technology2022，，13： | 3 |
| 4 | Characteristics of light-harvesting II mutant of Rhodobacter sphaeroides with alterations at the transmembrane helices ofβ-subunit显示文摘 | Wanneng Wang Zongli Hu Jinzhe Li | 2009 | Biochemistry(Moscow)2009，74，7： | 1 |
| 5 | Expression characterization and actual function of the second puc BA in Rhodobacter sphaeroides显示文摘 | Wanneng Wang Zongli Hu Jinzhe Li | 2009 | Bioscience Reports2009，，29： | 1 |
| 6 | Heterologous synthesis and assembly of functional LHII antenna complexes from Rhodovulum sulfidophilum in Rhodobacter sphaeroides mutant显示文摘 | Wanneng Wang Zongli Hu Xuqing Chen Zhiping Zhao Jinzhe Li Guoping Chen | 2009 | Molecular Biology Reports2009，，7： | 1 |
| 7 | Characteristics of light-harvesting complex II mutant of Rhodobacter sphaeroides with alterations at the transmembrane helices of β-subunit显示文摘 | Wanneng Wang Zongli Hu Jinzhe Li Xuqing Chen Guoping Chen | 2009 | Biochemistry (Moscow)2009，，7： | 1 |
| 8 | Min-Min Chromosome Genetic Algorithm for Load Balancing in Grid Computing显示文摘 | Shu Wanneng Wang Jianqing | 2009 | International Journal of Distributed Sensor Networks2009，5，1： | 1 |
| 9 | The Origin of the Black Shale Series and Bentonites from the Wufeng Formation in the Southwestern Upper Yangtze:Implications for the Convergence of the Yangtze and Cathaysia Blocks in the Late Ordovician显示文摘Early Paleozoic black organic sediments and bentonites occur widely in the craton basin within the Yangtze block and are generally believed to be genetically related to a specific tectonic setting on the cratonic boundary.However,the intimate relationship between their origins and the dynamic mechanisms are unclear,as exemplified by the genesis of the black shale series and bentonites from the Wufeng Formation during the Ordovician–Silurian transition(OST).In order to reveal the relationship between the Wufeng Formation and the convergence of the Yangtze and Cathaysia blocks(i.e.,the intracontinental Kwangsian Orogeny),two stratigraphic sections respectively in Zhaotong area(Northeast Yunnan)and Puyi area(Northwestern Guizhou)that were located in the semi-restricted inner Yangtze Sea during the OST were systematically studied,on the basis of whole-rock geochemical composition,pyriteδ^(34)S(δ^(34)Spy),total organic carbon(TOC),stable Sr isotope,pyrite framboid size distribution and zircon U-Pb age,trace elements.The evidence shows that the paleo-oceanic environment changed significantly at the turn of the early–late Katian and formed the black shale series in the Wufeng Formation.These acritarch assemblages were formed in the transition process of the Upper Yangtze Basin from passive continental margin basin to foreland basin during this interval.Based on previous research on the genetic relationship between black shale series and plate tectonic movement,a basin-mountain evolution model suitable for South China in the Late Ordovician is presented.The two bentonites in the Wufeng Formation with U-Pb ages of 445.5±0.8 Ma and 441.9±2.4 Ma primarily originated from the intermediate–acid volcanic eruption during the collision and convergence between the Yangtze and Cathaysia blocks in the Late Ordovician,the provenance region probably being located in the Jiangnan orogenic belt.Thus,we believe that the appearance of the black shale series and bentonite in the Wufeng Formation at the turn of the early–late Katian may represent the initiation of basin-mountain transformation and the Kwangsian Orogeny in South China,which provides important evidence for the collision and convergence of the Yangtze and Cathaysia blocks in the Late Ordovician. | MIAO Yu ZHOU Ye ZHOU Jiaxi TIAN Yufeng WU Liang LI Shizhong ZHANG Yaotang WANG Wanneng | 2022 | Acta Geologica Sinica(English Edition)2022，96，6： | 0 |
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