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| 1 | Use of siRNA molecular beacons to detect and attenuate mycobacterial infection in macrophages显示文摘Tuberculosis is one of the leading infectious diseases plaguing mankind and is mediated by the facultative pathogen, Mycobacterium tuberculosis(MTB). Once the pathogen enters the body, it subverts the host immune defenses and thrives for extended periods of time within the host macrophages in the lung granulomas, a condition called latent tuberculosis(LTB). Persons with LTB are prone to reactivation of the disease when the body's immunity is compromised. Currently there are no reliable and effective diagnosis and treatment options for LTB, which necessitates new research in this area. The mycobacterial proteins and genes mediating the adaptive responses inside the macrophage is largely yet to be determined. Recently, it has been shown that the mce operon genes are critical for host cell invasion by the mycobacterium and for establishing a persistent infection in both in vitro and in mouse models of tuberculosis. The Yrb E and Mce proteins which are encoded by the MTB mce operons display high degrees of homology to the permeases and the surface binding protein of the ABC transports, respectively. Similarities in structure and cell surface location impute a role in cell invasion at cholesterol rich regions and immunomodulation. The mce4 operon is also thought to encode a cholesterol transport system that enables the mycobacterium to derive both energy and carbon from the host membrane lipids and possibly generating virulence mediating metabolites, thus enabling the bacteria in its long term survival within the granuloma. Various deletion mutation studies involving individual or whole mce operon genes have shown to be conferring varying degrees of attenuation of infectivity or at times hypervirulence to the host MTB, with the deletion of mce4 A operon gene conferring the greatest degree of attenuation of virulence. Antisense technology using synthetic si RNAs has been used in knocking down genes in bacteria and over the years this has evolvedinto a powerful tool for elucidating the roles of various genes mediating infectivity and survival in mycobacteria. Molecular beacons are a newer class of antisense RNA tagged with a fluorophore/quencher pair and their use for in vivo detection and knockdown of mR NA is rapidly gaining popularity. | Remo George Renata Cavalcante Celso Carvalho Jr Elyana Marques Jonathan B Waugh M Tino Unlap | 2015 | World Journal of Experimental Medicine2015，5，3： | 2 |
| 2 | Mechanisms of resistance to the cytotoxic effects of oxysterols in human leukemic cells显示文摘 | Claudia C Gregorio-King Tamara Gough Gavin J Van Der Meer Jane B Hosking Caryll M Waugh Janet L McLeod Fiona Mc Collier Mark A Kirkland | 2004 | Journal of Steroid Biochemistry and Molecular Biology2004，，3： | 1 |
| 3 | What good are pos-itive emotions in crises? A prospective study of resilience and emotions following the terrorist attacks on the United States on September 11 th, 2001 显示文摘 | Fredrickson B L Tugade M M Waugh C E | 2003 | J Pers Soc Psychol2003，84，2： | 1 |
| 4 | Genome sequence and analysis of the tuber crop potato显示文摘 | Xu X Pan S Cheng S Zhang B Mu D Ni P Zhang G Yang S Li R Wang J Orjeda G Guzman F Tones M Lnzano R Ponce O Martinez D De la Cruz G Chakrabarti S K Patil V U Skryabin K G Kuznetsov B B Ravin N V Kolganova T V Beletsky A V Mardanov A V Di Genova A Bolser D M Martin D M Li G Yang Y Kuang H Hu Q Xiong X Bishop G J Sagredo B Mejia N Zagorski W Gromadka R Gawor J Szczesny P Huang S Zhang Z Liang C He J Li Y He Y Xu J Zhang Y Xie B Du Y Qu D Bonierbale M Ghislain M Herrera Mdel R Giuliano G Pietrella M Perrotta G Faeella P O 'Brien K Feingold S E Barreiro L E Massa G A Diambra L Whitty B R Vaillancourt B Lin H Massa A N Geoffroy M Lundback S DellaPenna D Buell C R Sharma S K Marshall D F Waugh R Bryan G J Destefanis M Nagy I Milbourne D Thomson S J Fiefs M Jacobs J M Nielsen K L Sonderkaer M Iovene M Tortes G A Jiang J Veilleux R E Bachem C W de Boer J Bonn T Kloosterman B van Eck H Datema E Hekkert B Goverse A van Ham R C Visser R G | 2011 | Nature2011，475，7355： | 1 |
| 5 | Smile to see the forest: Facially expressed positive emotions broaden cognition显示文摘 | Johnson K J Waugh C E Fredrickson B L | 2010 | Cognition and Emotion2010，24，2： | 1 |
| 6 | Totacco etch virusprotease:mechanism of autolysis and rational design ofstable mutants with wild-type catalytic proficiency显示文摘 | Kapust R B Copeland T D Waugh D S | 2001 | Protein Eng2001，14，2： | 1 |
| 7 | Escherichia coli maltose-bindingprotein is uncommonly effective at promoting the solubili-ty of polypeptides to which it is fused显示文摘 | Kaust R B Waugh D S | 1999 | Protein Sci1999，8，8： | 1 |
| 8 | Genetic distribution of Bare-1- like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP)显示文摘 | WAUGH R MCLEAN K FLAVELL A J PEARCE S R KUMAR A THOMAS B B T POWELL W | 1997 | Mol Gen Genet1997，253，： | 1 |
| 9 | Processive degra-dation of nascent polypeptides,triggered by tandem AGAcodons,limits the accumulation of recombinant tobaccoetch virus protease in Escherichia coli BL21(DE3)显示文摘 | Kapust R B Routzahn K M Waugh D S | 2002 | Protein Expr Purif2002，24，1： | 1 |
| 10 | Mucinous carcinomas of the breast:imaging features and potential for misdiagnosis 显示文摘 | Tan JZ Waugh J Kumar B | 2013 | J Med Imaging Radiat Oncol2013，57，1： | 1 |
| 11 | Intrusions into the tropical upper troposphere: Three-dimensional structure and accompanying ozone and OLR distributions显示文摘 | WAUGH D W FUNATSU B M | 2003 | J Atmos Sci2003，60，4： | 1 |
| 12 | What good are positive emotions in crises? A prospective study of resilience and emotions following the terrorist attacks on the United States on September llth, 2001显示文摘 | FREDRICKSON B L TUGADE M M WAUGH C E | 2003 | Journal of Personality & Social Psychology2003，84，2： | 1 |
| 13 | Mucinous carcinomas of thebreast : imaging features and potential for misdiagnosis 显示文摘 | Tan JZ Waugh J Kumar B | 2013 | J MedImaging Radiat Oncol2013，57，1： | 1 |
| 14 | Travoprost 显示文摘 | Waugh J Jarvis B | 2002 | Drugs Aging2002，19，6： | 1 |
| 15 | Authigenic iron and titanium oxides in Triassic red beds (St Bees Sandstone), Cumbria, northern England显示文摘 | Ixer R A Turner P Waugh B | 1979 | Geological Journal1979，14，1： | 1 |
| 16 | Smile to see the forest:facially expressed positive emotions broaden cognition显示文摘 | Johnson K J Waugh C E Fredrickson B L | 2010 | Cognition and Emotion2010，24，2： | 1 |
| 17 | Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex显示文摘 | Graner A Streng S Kellermann A Schiemann A Bauer E Waugh R Pellio B Ordon F | 1999 | Theoretical and Applied Genetics1999，98，2： | 1 |
| 18 | Bioassay of Rauwolfia roots and alkaloids 显示文摘 | RUBIN B MALONE M H WAUGH J C | 1957 | J Pharmacol Exp Ther1957，120，9： | 1 |
| 19 | Functional genomics of cell elongation in developing cotton fiber 显示文摘 | Arpat A B Waugh M Sullivan J P | 2004 | Plant Mol Biol2004，54，： | 1 |
| 20 | What good arepositive emotions in crisisg A prospective study of resilience and e - motions following the terrorist attacks on the United States onSeptember llth, 2001 显示文摘 | Fredrickson B L Tugade M M Waugh C E | 2003 | Journal of Personality and Social Psy - chology2003，84，2： | 1 |
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