|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Fluid Flow in Tundish Due to Different Type Arrangement of Weir and Dam显示文摘Tundish is an important metallurgical reactor in the continuous casting process. In order to control the fluid flow in tundish and thus take full advantage of the residence time available for the removal of inclusions from molten steel, the effect of weir and dam on the fluid flow has been studied in a water model based on the characteristic number Froude and Reynold number similarity criteria. The residence time distribution curves of the flow were measured by SG800, The optimum arrangement of dam and weir and the non- stationary flow in tundish were discussed. The results show that the combination of weir and dam is benefit for the flow pattern in tundish, weir can prevent the upper recirculating flow, dam can cut off the bottom flow and turn to upwards, it is advantageous to separate the nonmetallic inclusions. Furthermore, it is important to exceed the critical depth of bath during exchange ladles, not only for the inclusion floatation but also for avoiding tundish slag drainage earlier. | Yanping Bao, Wei Jiang,Baomei Xu, Haizhong Zhang Metallurgy School, University of Science and Technology Beijing, Beijing 100083, China E-mail: baoyp@metall .ustb .edu. cn | 2002 | Journal of University of Science and Technology Beijing2002，9，1： | 8 |
| 2 | Single-cell analysis reveals bronchoalveolar epithelial dysfunction in COVID-19 patients显示文摘Dear Editor,In 2019,a zoonotic coronavirus named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)was identified as the causative agent of Coronavirus Disease 2019(COVID-19).As of 8 June 2020,the World Health Organization(WHO)has reported 6,912,751 globally confirmed cases with 400,469 deaths.Although generally causes mild disease,SARS-CoV-2 infection can result in serious outcomes,including acute lung injury(ALI)and acute respiratory distress syndrome(ARDS),the leading cause of mortality in patients with comorbidities.Recent autopsy studies of COVID-19 patients revealed mononuclear infiltration and excessive production of mucus in the infected lung,especially in the damaged small airways and alveoli(Bian and Team,2020;Liu et al.,2020). | Jiangping He Shuijiang Cai Huijian Feng Baomei Cai Lihui Lin Yuanbang Mai Yinqiang Fan Airu Zhu Huang Huang Junjie Shi Dingxin Li' Yuanjie Wei Yueping Li Yingying Zhao’ Yuejun Pan He Liu Xiaoneng Mo Xi He Shangtao Cao FengYu Hu Jincun Zhao Jie Wang Nanshan Zhong Xinwen Chen Xilong Deng Jiekai Chen | 2020 | Protein & Cell2020，11，9： | 7 |
| 3 | Impact of bacterial chaperonin GroEL-GroES on bacteriorhodopsin folding and membrane integration显示文摘Our understanding of molecular chaperone function in membrane protein biogenesis lags far behind that in soluble protein biogenesis. Through a combined approach including isothermal titration calorimetry, UV-Vis spectroscopy, and fluorescence spectroscopy, the behavior of ATP-dependent chaperonin GroEL-GroES, a paradigmatic chaperone of soluble protein folding, was investigated in the refolding of membrane protein bacteriorhodopsin (BR) and its membrane insertion. We found that BR bound asymmetrically to the double-ring GroEL, w让h a much higher affinity when it was partially denatured. GroEL alone showed a clear influence on BR refolding, but the presence of ATP was necessary to significantly enhance both the rate and yield of the GroEL-mediated folding, in contrast to the adverse effect of GroES on the folding yield. However;synergy between ATP and GroES was shown to be required not only for releasing high-affinity BR species from GroEL, but also for unfolding and rescuing the misfolded conformers complexed to GroEL. This is consistent with the observation that maximum rate enhancement of BR refolding or assembly with the prepared inverted membrane vesicles was achieved when the complete chaperonin system was used. Our results support the iterative unfolding mechanism of GroEL activity previously proposed for soluble proteins, whereby GroEL might perform repeated unfolding and release of BR, thus offering additional opportunities for timely folding or membrane integration. This work provides important information on the convergence of folding of membrane and soluble proteins in light of folding pathways and the role of molecular chaperones. | Xinwei Lu Baomei Xu Haiyan Sun Junting Wei Haixia Chi Naseer Ullah Khan Xiaojuan Wang Xiaoqiang Wang Fang Huang | 2019 | Biophysics Reports2019，5，3： | 0 |
| 4 | Disrupted Maturation of Prefrontal Layer 5 Neuronal Circuits in an Alzheimer’s Mouse Model of Amyloid Deposition显示文摘Mutations in genes encoding amyloid precursor protein(APP)and presenilins(PSs)cause familial forms of Alzheimer’s disease(AD),a neurodegenerative disorder strongly associated with aging.It is currently unknown whether and how AD risks affect early brain development,and to what extent subtle synaptic pathology may occur prior to overt hallmark AD pathology.Transgenic mutant APP/PS1 over-expression mouse lines are key tools for studying the molecular mechanisms of AD pathogenesis.Among these lines,the 5XFAD mice rapidly develop key features of AD pathology and have proven utility in studying amyloid plaque formation and amyloidβ(Aβ)-induced neurodegeneration.We reasoned that transgenic mutant APP/PS1 over-expression in 5XFAD mice may lead to neurodevelopmental defects in early cortical neurons,and performed detailed synaptic physiological characterization of layer 5(L5)neurons from the prefrontal cortex(PFC)of 5XFAD and wild-type littermate controls.L5 PFC neurons from 5XFAD mice show early APP/Aβimmunolabeling.Whole-cell patch-clamp recording at an early post-weaning age(P22–30)revealed functional impairments;although 5XFAD PFC-L5 neurons exhibited similar membrane properties,they were intrinsically less excitable.In addition,these neurons received smaller amplitude and frequency of miniature excitatory synaptic inputs.These functional disturbances were further corroborated by decreased dendritic spine density and spine head volumes that indicated impaired synapse maturation.Slice biotinylation followed by Western blot analysis of PFC-L5 tissue revealed that 5XFAD mice showed reduced synaptic AMPA receptor subunit GluA1 and decreased synaptic NMDA receptor subunit GluN2A.Consistent with this,patch-clamp recording of the evoked L23>L5 synaptic responses revealed a reduced AMPA/NMDA receptor current ratio,and an increased level of AMPAR-lacking silent synapses.These results suggest that transgenic mutant forms of APP/PS1 overexpression in 5XFAD mice leads to early developmental defects of cortical circuits,which could contribute to the age-dependent synaptic pathology and neurodegeneration later in life. | Chang Chen Jing Wei Xiaokuang Ma Baomei Xia Neha Shakir Jessica K.Zhang Le Zhang Yuehua Cui Deveroux Ferguson Shenfeng Qiu Feng Bai | 2023 | Neuroscience Bulletin2023，39，6： | 0 |
| 5 | Reaction Performance of FCC Catalyst for Cracking Inferior Crude Oils显示文摘Driven by the increasing supply of heavy oils with deteriorating quality,a high nickel-resistant catalyst for catalytic cracking of inferior crude oils was developed by the Research Institute of Petroleum Processing(RIPP).Catalyst performance was evaluated in a laboratory fixed fluidized bed reactor.The test results showed that the high nickel resistance catalyst exhibited good bottoms crackability,good nickel resistance,and good adaptability to changes in operating parameters,which had no adverse effect on the product distribution,indicating to a most promising prospect for application of this catalyst in catalytic cracking of inferior crude oil. | Wei Xiaoli Song Baomei Mao Anguo Long Jun (SINOPEC Research Institute of Petroleum Processing,Beijing 100083) | 2009 | China Petroleum Processing & Petrochemical Technology2009，11，4： | 0 |
| 6 | Comparison of Three Methods for Determination of Ammonia Nitrogen in Surface Water显示文摘Nessler's reagent spectrophotometry,gas phase molecular absorption spectrometry and ion chromatography were respectively used to determine ammonia nitrogen in surface water.The detection limit,precision,accuracy and recovery rate of the three methods were compared,and the determination results of the actual water samples were analyzed by t test.The results showed that the three methods all could meet the requirement of the laboratory quality control,and there was no significant difference in the determination results.Compared with Nessler's reagent spectrophotometry,the operation of gas phase molecular absorption spectrometry and ion chromatography operation was more convenient and fast and has no pollution,which can be used for the determination of ammonia nitrogen content in a large quantity of surface water samples. | Zuo Jia Dai Shuhao Wei Baomei Qiu Lan Tu Xiaobin | 2019 | Meteorological and Environmental Research2019，10，3： | 0 |
| 7 | Development of a New Subermerged Entry Nozzle in Thin Slab Caster显示文摘The fluid flow in the mould of the thin slab continuous caster has a large intluence on the quality of slabs and its productivity.The fluid flow pattern can be controlled by the SEN (Submerged Entry Nozzle) structure. Traditional SEN can not decrease the surface turbulence and penetration depth at the same time, especially at high casting speed. In order to improve the fluid flow in the mould, a new structure SEN-Dissipation SEN have been invented. The water modeling experiments proved that the dissipation SEN could satisfy, the needs of fluid flow condition in the mould at high casting speed. | Yanping Bao Jianqiang Zhu Wei Jiang Naiyuan Tian Baomei Xu(Metallurgy School, University of Science & Technology Beijing, Beijing 100083, China) | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员