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| 1 | Atorvastatin protects against cerebral ischemia/ reperfusion injury through anti-inflammatory and antioxidant effects显示文摘In addition to its lipid-lowering effect, atorvastatin exerts anti-inflammatory and antioxidant effects as well. In this study, we hypothesized that atorvastatin could protect against cerebral ischemia/reperfusion injury. The middle cerebral artery ischemia/reperfusion model was established, and atorvastatin, 6.5 mg/kg, was administered by gavage. We found that, after cerebral ischemia/ reperfusion injury, levels of the inflammation-related factors E-selectin and myeloperoxidase were upregulated, the oxidative stress-related marker malondialdehyde was increased, and superoxide dismutase activity was decreased in the ischemic cerebral cortex. Atorvastatin pretreatment significantly inhibited these changes. Our findings indicate that atorvastatin protects against cerebral ischemia/reperfusion injury through anti-inflammatory and antioxidant effects. | Qiuyun Tu Hui Cao Wei Zhong Binrong Ding Xiangqi Tang | 2014 | Neural Regeneration Research2014，9，3： | 29 |
| 2 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 3 | Microvascular changes after conbercept therapy in central retinal vein occlusion analyzed by optical coherence tomography angiography显示文摘AIM: To investigate microvascular changes in eyes with central retinal vein occlusion(CRVO) complicated by macular edema before and after intravitreal conbercept injection and evaluate correlations between these changes and best-corrected visual acuity(BCVA) and retinal thickness. METHODS: Twenty-eight eyes of 28 patients with macular edema caused by CRVO were included in this retrospective study. All patients received a single intravitreal conbercept injection to treat macular edema. BCVA and the results of optical coherence tomography angiography(OCTA) automatic measurements of the vessel density in the superficial(SCP) and deep retinal capillary plexus(DCP), the foveal avascular zone(FAZ) area, the FAZ perimeter(PERIM), the vessel density within a 300-μm wide ring surrounding the FAZ(FD-300), the acircularity index(AI), the choriocapillaris flow area, and retinal thickness were recorded before and at one month after treatment and compared with the results observed in age-and sexmatched healthy subjects. RESULTS: The vessel density in the SCP and DCP, the FD-300, and the flow area of the choriocapillaris were allsignificantly lower in CRVO eyes than in healthy eyes, while the AI and retinal thickness were significantly higher(all P<0.05). After treatment, retinal thickness was significantly decreased, and the mean BCVA had markedly improved from 20/167 to 20/65(P=0.0092). The flow area of the choriocapillaris was also significantly improved, which may result from the reduction of shadowing effect caused by the attenuation of macular edema. However, there were no significant changes in SCP and DCP vessel density after treatment. The flow area of the choriocapillaris at baseline was negatively correlated with retinal thickness.CONCLUSION: OCTA enables the non-invasive, layerspecific and quantitative assessment of microvascular changes both before and after treatment, and can therefore be used as a valuable imaging tool for the evaluation of the follow-up in CRVO patients. | Yang Deng Qi-Wen Zhong Ai-Qi Zhang Xiao-Juan Cai Ming-Zhi Lu Shao-Chong Zhang Li-Shi Su Hui Chen Ying Lin Li-Mei Sun Guan-Di Chen Li-Ting Zhong Chen-Jin Jin Wei Chi | 2019 | International Journal of Ophthalmology(English edition)2019，12，5： | 13 |
| 4 | A 12-year cohort study on the efficacy of plasmaderived hepatitis B vaccine in rural newborns显示文摘AIM To understand the anti-HBs persistenceand the long-term preventive efficacy in ruralnewborns after vaccination with plasma-derivedhepatitis B vaccine.METHODS In the time of expanded program onimmunization(EPI),the newborns werevaccinated with 10μg×3 doses of hepatitis Bvaccine and 762 newborns who were HBsAgnegative after primary immunization wereselected for cohort observation from 1986 to1998.Their serum samples were detectedqualitatively and quantitatively for hepatitis Binfecting markers,including HBsAg,anti-HBsand anti-HBc by SPRIA Kits.The annual HBsAgpositive conversion rate was counted by life-table method.RESULTS①The anti-HBs positive rate was94.44% for the babies born to HBsAg negativemothers and 84.21% for those born to HBsAgpositive mothers in the 1st year afterimmunization,and dropped to 51.31% and52.50% in the 12th year respectively.GMT valuewas dropped from 31.62 to 3.13 and 23.99 to 3.65in the 2nd to the 12th year respectively.Therewas a marked drop in GMT at the 3rd to the 5thyear,and in anti-HBs positive rate at the 9th tothe 10th year.②In the period of 12 yearsobservation,the person-year HBsAg positive conversion rates were 0.12%(5/4150.0)innewborns born to HBsAg negative mothers and0.20%(1/508.0)in those born to HBsAgpositive mothers,and none of the HBsAgpositive converted children became HBsAgchronic carriers.Compared with the baselinebefore immunization,the protective rates were97.19% and 95.32% respectively.CONCLUSION The protective efficacy ofplasma-derived hepatitis B vaccine persisted atleast 12 years,and a booster dose seems notnecessary within at least 12 years after theprimary three-doses immunization to newbornsborn to HBsAg negative mothers. | Hong Bin Liu Zong Da Meng Jing Chen Ma Chang Quan Han Ying Lin Zhang Zhan Chun Xing Yu Wei Zhang Yu Zhong Liu Hui Lin Cao | 2000 | World Journal of Gastroenterology2000，6，3： | 11 |
| 5 | Effect of a Traditional Chinese Medicine combined therapy on adolescent idiopathic scoliosis:a randomized controlled trial显示文摘OBJECTIVE:To evaluate the effectiveness of a combined Traditional Chinese Medicine(TCM) therapy versus conventional treatment on adolescent idiopathic scoliosis.METHODS:One hundred twenty outpatients with mild and moderate adolescent idiopathic scoliosis were randomly divided into a TCM group(TCMG)and a brace group(CG).TCMG patients underwent Daoyin,Tuina,and acupotomology therapies.CG patients were treated with a Milwaukee brace.Each patient's Cobb angle was measured after 12 and 24 months of treatment,and pulmonary function was determined after 12 months of treatment.Average electromyogram(AEMG) ratio of the surface electromyogram was measured after 6 and 12 months of treatment and followed-up after 18 and 24 months.RESULTS:The Cobb angle significantly decreased in both groups after 12 months of treatment compared with before treatment(P< 0.05).The percentages of original Cobb angle in TCMG and CG were51.4%and 47.8%(P > 0.05) after 12 months and62.5%and 34.7%(P < 0.05) after 24 months,respectively.Pulmonary function significantly improved after 12 months in TCMG(P < 0.05) but significantly decreased in CG(P < 0.05).The AEMG ratio was significantly lower(P < 0.01) and tended to remain at1 after stopping treatment in TCMG,but increased in CG(P<0.05).CONCLUSION:TCM combined therapy can prevent the progression of scoliosis.The AEMG ratio is a promising index that could replace radiography in the evaluation of treatment effect and progression in scoliosis. | Wei Hui Xu Jinyuan Jiang Zhong Ye Shuliang Song Hongquan Ning Xitao Huang Huanmin Chen Wei Pei Jianwei Jiang Nengyi Chen Shao Du Honggen | 2015 | Journal of Traditional Chinese Medicine2015，35，5： | 9 |
| 6 | Crust and Upper Mantle Electrical Resistivity Structure in the Panxi Region of the Eastern Tibetan Plateau and Its Significance显示文摘The Panxi region is located in the frontal zone of positive squeezing subduction and side squeezing shearing between the Indian plate and the Eurasian plate. The long-period magnetotelluric(LMT) and broadband magnetotelluric(MT) techniques are both used to study the deep electrical conductivity structure in this region; magnetic and gravity surveys are also performed along the profile. According to the 2-D resistivity model along the Yanyuan-Yongshan profile,a highconductivity layer(HCL) exists widely in the crust,and a high-resistivity block(HRB) exists widely in the upper mantle in general,as seen by the fact that a large HCL exists from the western Jinpingshan tectonic zone to the eastern Mabian tectonic zone in the crust,while the HRB found in the Panxi tectonic zone is of abnormally high resistivity in that background compared to both sides of Panxi tectonic zone. In addition,the gravity and magnetic field anomalies are of high value. Combined with geological data,the results indicate that there probably exists basic or ultrabasic rock with a large thickness in the lithosphere in the Panxi axial region,which indicates that fracture activity once occurred in the lithosphere. As a result,we can infer that the high-resistivity zone in the Panxi lithosphere is the eruption channel for Permian Emeishan basalt and the accumulation channel for basic and ultrabasic rock. The seismic sources along the profile are counted according to seismic record data. The results indicate that the most violent earthquake sources are located at the binding site of the HRB and the HCL,where the tectonic activity zone is generally acknowledged to be; however,the earthquakes occurring in the HCL are not so violent,which reflects the fact that the HCL is a plastic layer,and the fracture threshold of a plastic layer is low generally,making high stress difficult to accumulate but easy to release in the layer. As a result,a higher number of smaller earthquakes occurred in the HCL at Daliangshan tectonic zone,and violent earthquakes occurred at the binding site of high- and low-resistivity blocks at the Panxi tectonic zone. | ZHANG Gang WANG Xuben FANG Hui GUO Ziming ZHANG Zhaobin LUO Wei CAI Xuelin LI Jun LI Zhong WU Xing | 2015 | Acta Geologica Sinica(English Edition)2015，89，2： | 9 |
| 7 | Transarterial chemoembolization with PD-(L)1 inhibitors plus molecular targeted therapies for hepatocellular carcinoma(CHANCE001)显示文摘There is considerable potential for integrating transarterial chemoembolization(TACE),programmed death-(ligand)1(PD-[L]1)inhibitors,and molecular targeted treatments(MTT)in hepatocellular carcinoma(HCC).It is necessary to investigate the therapeutic efficacy and safety of TACE combined with PD-(L)1 inhibitors and MTT in real-world situations.In this nationwide,retrospective,cohort study,826 HCC patients receiving either TACE plus PD-(L)1 blockades and MTT(combination group,n=376)or TACE monotherapy(monotherapy group,n=450)were included from January 2018 to May 2021.The primary endpoint was progression-free survival(PFS)according to modified RECIST.The secondary outcomes included overall survival(OS),objective response rate(ORR),and safety.We performed propensity score matching approaches to reduce bias between two groups.After matching,228 pairs were included with a predominantly advanced disease population.Median PFS in combination group was 9.5 months(95%confidence interval[CI],8.4-11.0)versus 8.0 months(95%CI,6.6-9.5)(adjusted hazard ratio[HR],0.70,P=0.002).OS and ORR were also significantly higher in combination group(median OS,19.2[16.1-27.3]vs.15.7 months[13.0-20.2];adjusted HR,0.63,P=0.001;ORR,60.1%vs.32.0%;P<0.001).Grade 3/4 adverse events were observed at a rate of 15.8%and 7.5%in combination and monotherapy groups,respectively.Our results suggest that TACE plus PD-(L)1 blockades and MTT could significantly improve PFS,OS,and ORR versus TACE monotherapy for Chinese patients with predominantly advanced HCC in real-world practice,with an acceptable safety profile. | Hai-Dong Zhu Hai-Liang Li Ming-Sheng Huang Wei-Zhu Yang Guo-Wen Yin Bin-Yan Zhong Jun-Hui Sun Zhi-Cheng Jin Jian-Jian Chen Nai-Jian Ge Wen-Bin Ding Wen-Hui Li Jin-Hua Huang Wei Mu Shan-Zhi Gu Jia-Ping Li Hui Zhao Shu-Wei Wen Yan-Ming Lei Yu-Sheng Song Chun-Wang Yuan Wei-Dong Wang Ming Huang Wei Zhao Jian-Bing Wu Song Wang Xu Zhu Jian-Jun Han Wei-Xin Ren Zai-Ming Lu Wen-Ge Xing Yong Fan Hai-Lan Lin Zi-Shu Zhang Guo-Hui Xu Wen-Hao Hu Qiang Tu Hong-Ying Su Chuan-Sheng Zheng Yong Chen Xu-Ya Zhao Zhu-Ting Fang Qi Wang Jin-Wei Zhao Ai-Bing Xu Jian Xu Qing-Hua Wu Huan-Zhang Niu Jian Wang Feng Dai Dui-Ping Feng Qing-Dong Li Rong-Shu Shi Jia-Rui Li Guang Yang Hai-Bin Shi Jian-Song Ji Yu-E Liu Zheng Cai Po Yang Yang Zhao Xiao-Li Zhu Li-Gong Lu Gao-Jun Teng | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 7 |
| 8 | Highly efficient base editing in human tripronuclear zygotes显示文摘 | Changyang Zhou Meiling Zhang Yu Wei Yidi Sun Yun Sun Hong Pan Ning Yao Wanxia Zhong Yixue Li Weiping Li3 Hui Yang Zi-jiang Chen | 2017 | Protein & Cell2017，8，10： | 6 |
| 9 | Hepatitis C Virus Infection Downregulates the Ligands of the Activating Receptor NKG2D显示文摘Natural killer (NK) cells are a major component of the host innate immune defense against various pathogens. Several viruses, including hepatitis C virus (HCV), have developed strategies to evade the NK-cell response. In our study, we found HCV infection could trigger DNA damage response by both ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) pathways. Recent reports had revealed that NKG2D ligands (NK cell-activating ligands) were upregulated when a major DNA damage checkpoint pathway was activated. However, here we found that DNA damage response was activated but NKG2D ligands were downregulated upon HCV infection. Further studies showed that the protease NS3/4A of HCV which had been shown relation with immune invasion contributed to the reduced expression of NKG2D ligands. These findings provide a novel insight into the mechanisms evolved by HCV to escape from the NK cell response. | Chaoyang Wen Xiang He Hongfang Ma Ningbo Hou Congwen Wei Ting Song Yanhong Zhang Liping Sun Qingjun Ma Hui Zhong | 2008 | Cellular & Molecular Immunology2008，5，6： | 6 |
| 10 | Effects of chlortetracycline,Cu and their combination on the performance and microbial community dynamics in swine manure anaerobic digestion显示文摘Swine manure was typical for the combined pollution of heavy metals and antibiotics. The effects of widely used veterinary antibiotic chlortetracycline(CTC), Cu and their combination on swine manure anaerobic digestion performance and microbial community have never been investigated. Thus, four 2 L anaerobic digestion reactors were established including reactor A(control), B(CTC spiked by 0.5 g/kg dry weight, dw), C(Cu spiked by 5 g/kg dw) and D(combination of CTC, 0.5 g/kg dw, and Cu, 5 g/kg dw), and dynamics of bacterial and archaeal community structure was investigated using high throughput sequencing method. Results showed that addition of CTC and Cu separately could increase the total biogas production by21.6% and 15.8%, respectively, while combination of CTC and Cu severely inhibited anaerobic digestion(by 30.3%). Furthermore, corresponding to different stages and reactors, four kinds of microbes including bacteria and archaea were described in detail, and the effects of CTC, Cu and their combination mainly occurred at hydrolysis and acidification phases. The addition of Cu alone changed the dynamics of archaeal community significantly. It was genus Methanomassiliicoccus that dominated at the active methane production for A, B and D, while it was genus Methanobrevibacter and Methanoculleus for C. | Rui Wang Junya Zhang Jibao Liu Dawei Yu Hui Zhong Yawei Wang Meixue Chen Juan Tong Yuansong Wei | 2018 | Journal of Environmental Sciences2018，30，5： | 5 |
| 11 | A New Somphospondylan Sauropod (Dinosauria, Titanosauriformes) from the Late Cretaceous of Ganzhou, Jiangxi Province of Southern China显示文摘A new somphospondylan sauropod (Dinosauria, Titanosauriformes): Gannansaurus sinensis gen. et sp. nov. from the Late Cretaceous Nanxiong Formation of Ganzhou Basin, Jiangxi Province of southern China is erected based on one nearly complete dorsal vertebra and a mid-caudal vertebra. It is characterized by two centroprezygapophyseal lamina fossae; large, square-shaped concavity formed by centroprezygapophyseal lamina and dorsal margin of the centrum; large infraparapophyseal fossa; three openings present within the large lateral concavity, which occupy 65% length of the centrum; the posterior centroparapophyseal lamina (PCPL) and the anterior centroparapophyseal lamina (ACPL) weakly developed; the posterior centrodiapophyseal lamina (PCDL) separated by an infradiapophyseal fossa into two branches dorsally; parapophyseal and diapophyseal laminae cross to form 'K' configuration. It shares some characters with the Early Cretaceous form-Euhelopus, indicating that it is more closely related to Euhelopus rather than to other titanosauriform sauropods. This means Gannansaurus may have a close phylogenetic relationship with Euhelopus. | Lü Junchang YI Laiping ZHONG Hui WEI Xuefang | 2013 | Acta Geologica Sinica(English Edition)2013，87，3： | 5 |
| 12 | Dynamics of Rodent and Rodent-borne Disease during Construction of the Three Gorges Reservoir from 1997 to 2012显示文摘Objective To investigate the impact of impoundment and active public health interventions on rodent populations and rodent-borne diseases in the Three Gorges reservoir region from 1997 to 2012. Methods Surveillance data from 1997 to 2012 were extracted from the Public Health Surveillance System of The Three Gorges established in 1997. Temporal changes in the incidences of hemorrhagic fever with renal syndrome(HFRS) and leptospirosis, rodent density, pathogen-carrying rates, and their correlations were analyzed. Results The average indoor and outdoor rodent densities decreased overall from 1997 to 2012. The average densities decreased by 47.72%(from 4.38% to 2.29%) and 39.68%(from 4.41% to 2.66%), respectively, after impoundment(2003-2012) compared with before impoundment(1997-2002). The average annual incidence rates of HFRS and leptospirosis were 0.29/100,000 and 0.52/100,000, respectively, and decreased by 85.74%(from 0.68/100,000 to 0.10/100,000) and 95.73%(from 1.47/100,000 to 0.065/100,000), respectively, after impoundment compared with before impoundment. Incidences of HFRS and leptospirosis appear to be positively correlated with rodent density in the reservoir area. Conclusion This study demonstrated that rodent density and incidences of rodent-borne diseases decreased and were maintained at low levels during construction of the Three Gorges dam. Measures that reduce rodent population densities could be effective in controlling rodent-borne diseases during large-scale hydraulic engineering construction. | CHANG Zhao Rui LU Liang MAO De Qiang PAN Hui Ming FENG Lian Gui YANG Xiao Bing LIU Feng Feng HE Yuan Yuan ZHANG Jing YANG Wei Zhong | 2016 | Biomedical and Environmental Sciences2016，29，3： | 5 |
| 13 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 14 | Effect of organic matter on phosphorus recovery from sewage sludge subjected to microwave hybrid pretreatment显示文摘Microwave(MW) hybrid processes are able to disrupt the flocculent structure of complex waste activated sludge,and help promote the recovery of phosphorus as struvite.In this study,to optimize struvite yield,(1) the characteristics of matter released in MW-hybrid treatments were compared,including MW,MW-acid,MW-alkali,MW-H_2O_2,and MW-H_2O_2- alkali.The results showed that selective release of carbon,nitrogen,phosphorus,Ca^(2+),and Mg^(2+) achieved by sludge pretreatment using MW-hybrid processes.MW-H_2O_2 is the recommended sludge pretreatment process for phosphorus recovery in the form of struvite.The ratio of Mg^(2+):NH_4^+-N:PO_4^(3-)-P was 1.2:2.9:1 in the supernatant.(2) To clarify the effects of organic matter on struvite recovery,the composition and molecular weight distribution of organic matters were analyzed.Low molecular weight COD was found to facilitate the removal rate of NH_4^+-N and PO_4^(3-)P via crystallization,and the amorphous struvite crystals(<1 kDa) from the filtered solutions had high purity.Therefore,the present study reveals the necessity of taking into consideration the interference effect of high molecular weight organic matters during struvite crystallization from sewage sludge. | Yawei Wang Qingcong Xiao Hui Zhong Xiang Zheng Yuansong Wei | 2016 | Journal of Environmental Sciences2016，28，1： | 4 |
| 15 | Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies显示文摘A fluorescence polarization immunoassay(FPIA) for the determination of salinomycin(SAL) was developed by using anti-SAL monoclonal antibodies(mAb).Fluorescein labeled SAL(tracer) was synthesized by the N-hydroxysuccinimide active ester method and purified using thin layer chromatography(TLC).The developed FPIA for SAL had a dynamic range from 0.60 to 2193 ng/mL with an IC50 value of 33.2 ng/mL and a detection limit(LOD) of 0.08 ng/mL.No significant cross-reactivities were observed with other drugs but 67.6% with narasin. | Wang, Zhan Hui Cheng, Lin Li Shi, Wei Min Zhang, Su Xia Shen, Jian Zhong | 2010 | Science China Chemistry2010，53，3： | 4 |
| 16 | Ataxia telangiectasia-mutated-Rad3-related DNA damage checkpoint signaling pathway triggered by hepatitis B virus infection显示文摘AIM: To explore whether acute cellular DNA damage response is induced upon hepatitis B virus (HBV) infection and the effects of the HBV infection.METHODS: We incubated HL7702 hepatocytes with HBV-positive serum,mimicking a natural HBV infection process.We used immunoblotting to evaluate protein expression levels in HBV-infected cells or in non-infected cells; immunofluorescence to show ATR foci ands Chk1 phosphorylation foci formation; flow cytometry to analyze the cell cycle and apoptosis; ultraviolet (UV) radiation and ionizing radiation (IR)-treated cells to mimic DNA damage; and Trypan blue staining to count the viable cells.RESULTS: We found that HBV infection induced an increased steady state of ATR protein and increased phosphorylation of multiple downstream targets including Chk1,p53 and H2AX.In contrast to ATR and its target,the phosphorylated form of ATM at Ser-1981 and its downstream substrate Chk2 phosphorylation at Thr-68 did not visibly increase upon infection.However,the level of Mre11 and p21 were reduced beginning at 0.5 h after HBV-positive serum addition.Also,HBV infection led to transient cell cycle arrest in the S and the G2 phases without accompanying increased apoptosis.Research on cell survival changes upon radiation following HBV infection showed that survival of UV-treated host cells was greatly increased by HBV infection,owing to the reduced apoptosis.Meanwhile,survival of IR-treated host cells was reduced by HBV infection.CONCLUSION: HBV infection activates ATR DNA damage response to replication stress and abrogates the checkpoint signaling controlled by DNA damage response. | Fan Zhao Ning-Bo Hou Xiang He Yan-Hong Zhang Cong-Wen Wei Ting Song Li Li Qing-Jun Ma Hui Zhong Xiao-Li Yang Yu Liu | 2008 | World Journal of Gastroenterology2008，14，40： | 3 |
| 17 | Catalytic oxidation of 4-tert-butyltoluene over Ti-MCM-41显示文摘The surface-grafted titanium MCM-41 materials were prepared by anchoring titanocene onto the inner walls of MCM-41. The materials were characterized by powder X-ray diffraction (XRD), N2 adsorption-desorption isotherm and diffuse reflectance UV-visible (UV-vis) spectroscopies. The catalytic properties of Ti-MCM-41 were tested in oxidation of 4-tert-butyltoluene with tert-butylhydroperoxide (TBHP) in liquid phase. MCM-41 with loading 4.8 mol% Ti gave the maximal conversions of 23.6% of 4-tert-butyltoluene with a complete selectivity to 4-tert-butylbenzaldehyde. | Wei Hua Yu Chun Hui Zhou Xiang Sheng Xu Zhong Hua Ge | 2007 | Chinese Chemical Letters2007，18，3： | 3 |
| 18 | Defective molybdenum sulfide quantum dots as highly active hydrogen evolution electrocatalysts显示文摘铝二硫化物(瞬间 2), 为有二维的分层的结构的氢进化反应的有希望的非宝贵的 electrocatalyst,接受了在最近的年里增加注意。它的 electrocatalytic 表演被低活跃地点内容和差的电导率限制了。此处,我们报导一个灵巧、一般的 ultrafast 激光脱离方法综合为 electrocatalytic 的瞬间 2 量点(MS-QDs ) 她与充分暴露的活跃地点和高度提高的电导率。MS-QDs 被相应体积材料的 ultrafast 激光脱离在水的答案,他们部分在期间被氧化并且形成了有缺点的结构准备。同样准备的 MS-QDs 在 electrocatalytic 表明了高活动和稳定性她的由于他们的很大的表面区域,有缺点的结构,许多活跃地点,和高电导率。现在的 MS-QDs 能也在光学发现申请,察觉到,精力存储,和变换技术。 | Gang Ou Peixun Fan Xiaoxing Ke Yushuai Xu Kai Huang Hehe Wei Wen Yu Hongjun Zhang Minlin Zhong Hui Wu Yadong Li | 2018 | Nano Research2018，11，2： | 3 |
| 19 | Oncolytic adenovirus SG600-IL24 selectively kills hepatocellular carcinoma cell lines显示文摘AIM: To investigate the effect of oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus Ad.IL-24 on hepatocellular carcinoma (HCC) cell lines and normal liver cell line. METHODS: HCC cell lines (HepG2, Hep3B and MHCC97L) and normal liver cell line (L02) with a different p53 status were infected with SG600-IL24 and Ad.IL-24, respectively. Melanoma differentiation-associated (MDA)-7/interleukin (IL)-24 mRNA and protein expressions in infected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), enzymelinked immunosorbent assay (ELISA), and Western blotting, respectively. Apoptosis of HCC cells and normal liver cells was detected by cytometric assay with Hoechst33258 staining. 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay was used to investigate proliferation of HCC cells and normal liver cells, and cell cycle was assayed by flow cytometry. RESULTS: RT-PCR, ELISA and Western blotting showed that the exogenous MDA-7/IL-24 gene was highly expressed in cells infected with SG600-IL24. MTT indicated that SG600-IL24 could suppress the growth of HepG2, Hep3B, MHCC97L, with an inhibition rate of 75% ± 2.5%, 85% ± 2.0%, 72% ± 1.8%, respectively (P < 0.01), promote the apoptosis of HepG2, Hep3B, MHCC97L, with an apoptosis rate of 56.59% ± 4.0%, 78.36% ± 3.5%, 43.39% ± 2.5%, respectively (P < 0.01), and block the HCC cell lines in the G2/M phase with a blocking rate of 35.4% ± 4.2%, 47.3% ± 6.2%, 42% ± 5.0%, respectively (P < 0.01) but not the normal liver cell line in a p53-independent manner. CONCLUSION: SG600-IL24 can selectively suppress the proliferation and apoptosis of HCC cell lines in vitro but not normal liver cell line L02 in a p53-independent manner. Compared with Ad.IL-24, SG600-IL24 can significantly enhance the antitumor activity in HCC cell lines. | Xin-Bo Xue Chao-Wen Xiao Hui Zhang Ai-Guo Lu Wei Gao Zhu-Qing Zhou Xin-Lai Guo Ming-An Zhong Yao Yang Cong-Jun Wang | 2010 | World Journal of Gastroenterology2010，16，37： | 3 |
| 20 | Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries显示文摘冠的动脉把血流动带到心肌肉。理解冠的动脉的发展程序提供卓见进冠的动脉疾病的治疗。多重来源被描述了为包括 proepicardium,湾穴 venosus (SV ) ,和心内膜贡献冠的动脉。然而,冠的容器的发展起源仍然在强烈学习下面。我们为学习冠的开发生产了一个新基因工具,一只 AplnCreER 老鼠在开发冠的容器明确地衬里 recombinase,它表示可诱导的 Cre。冠的开发和 AplnCreER 命运跟踪的预定正式就职的定量分析证明 subepicardial endothelial 房间(EC ) 的子孙两个都入侵紧缩的心肌层形成冠的动脉并且在表面上留下生产静脉。我们发现这些 subepicardial EC 是 intramyocardial 的主要来源在发展中的心的冠的容器。在 vitro 将生物的活组织移植于培养基中培养,试金显示这些 subepicardial EC 的多数从 SV 和中庭的心内膜,然而并非从室的心内膜产生。Apln 积极的房间的同种细胞的分析显示单个 subepicardial EC 同等地作出贡献到冠的动脉和静脉。一起,这些数据建议 subepicardial EC 是 intramyocardial 的主要来源在室的冠的动脉墙,和那冠的动脉和静脉在发展中的心有普通起源。 | Xueying Tian Tianyuan Hu Hui Zhang Lingjuan He Xiuzhen Huang Qiaozhen Liu Wei Yu Liang He Zhongzhou Yang Zhen Zhang Tao P Zhong Xiao Yang Zhen Yang Yan Yan Antonio Baldini Yunfu Sun Jie Lu Robert J Schwartz Sylvia M Evans Adriana C Gittenberger-de Groot Kristy Red-Horse Bin Zhou | 2013 | Cell Research2013，23，9： | 3 |
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