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| 1 | Atg7 is required for acrosome biogenesis during spermatogenesis in mice显示文摘acrosome 是盖住精子原子核的前面的部分并且在授精的过程起一个必要作用的一个专业化细胞器。位于这个 lysosome 相关的细胞器(LRO ) 的生物的续生说下面的分子的机制大部分仍然是未知的。这里,我们显示出房间特定的 Atg7 大美人老鼠是的那个细菌由于在 acrosome 生物的续生说的一个缺点不肥沃并且显示了类似于人的 globozoospermia 的显型;这个繁殖缺点被 intracytoplasmic 精子注射成功地救。而且,在细菌房间的 Atg7 的弄空没影响细菌房间的开发的早阶段,但是精子发生在以后上演,在 Golgi 期间没有通过到保险丝进一个单个 acrosomal 泡的 proacrosomal 泡分阶段执行,它最后导致了不规则或将近 round-headed 精子。Autophagic 流动在弄空 Atg7 的细菌房间被破坏,最后导致 LC3 变化形式的失败到 Golgi 导出仪器的泡。另外, Atg7 部分调整了另一 globozoospermia 相关的蛋白质,联系 Golgi 的 PDZ 卷和卷卷包含主题的蛋白质(GOPC ) ,在 acrosome 生物的续生说期间。最后,任何一个 autophagy 的注射或进睾丸的 lysosome 禁止者导致了类似的显型到细菌的房间特定的 Atg7 大美人老鼠。总的来说,我们的结果揭开为在 acrosome 的生物的续生说的 Atg7 的一个新角色,并且我们提供证据为 acrosome 支持 autolysosome 起源假设。 | Hongna Wang Haifeng Wan Xixia Li Weixiao Liu Qi Chen Yaqing Wang Lin Yang Hongmei Tang Xiujun Zhang Enkui Duan Xiaoyang Zhao Fei Gao Wei Li | 2014 | Cell Research2014，24，7： | 25 |
| 2 | Spatio-temporal Changes of ≥10℃ Accumulated Temperature in Northeastern China since 1961显示文摘The objective of this study was to provide reliable basis for decision making for national food security and layout and structure adjustment of grain production in the northeastern China. The data of mean daily air temperature of 1961-2009 from 106 meteorological stations in the northeastern China were chosen in this study. Using statistical methods and isoline method, the spatio-temporal changes of various decadal ≥ 10℃ accumulated temperature and the climatic means of ≥ 10℃ accumulated temperature were studied in this paper. The results showed that 1) The geo-graphical distribution of ≥ 10℃ accumulated temperature in the northeastern China could be influenced directly by the latitude, longitude and altitude. If latitude moved one degree northward, the average decrease amplitude of the climatic means was 101.9℃ in the study area. 2) The means of decadal ≥ 10℃ accumulated temperature rose since the 1980s, and their increase amplitudes became larger in the 1990s and the 2010s obviously. Compared with those of the 1980s, ≥ 10℃ accumulated temperature increased by about 100℃ in the mountainous and plain areas in the 1990s; compared with those of the 1990s, ≥ 10℃ accumulated temperature increased by about 200℃ in the Hulun Buir High Plain and the Songnen Plain, and 100℃ in the Sanjiang Plain and the Liaohe Plain in the 2010s. 3) The means of the decadal ≥ 10℃ accumulated temperature for 106 meteorological stations in the northeastern China increased with the rate of 145.57℃/10yr in 1961-2009. 4) The climatic means of ≥ 10℃ accumulated temperature increased from 1961-1990 to 1971-2000 and 1981-2009. Compared with the climatic mean of 1971-2000, that of 1981-2009 had increased by above 50℃ in most of the study area, even up to 156℃. Compared with the climatic mean of 1961-1990, that of 1981-2009 increased by above 100℃ in most parts of the study area, even up to 200℃. 5) The maximum northward shift, eastward and westward extension amplitudes of 3100℃, 3300℃ and 3500℃ isolines were larger among all isoli-nes for the climatic means of the three phases. Compared with the positions of the isolines of 1961-1990, those ampli-tudes of 3100℃ isoline of 1981-2009 were 145 km, 109 km and 64 km, respectively; those of 3300℃ isoline were 154 km, 54 km and 64 km, respectively; and the maximum northward shift of 3500℃ isoline was about 100 km. | YAN Minhua LIU Xingtu ZHANG Wei LI Xiujun LIU Shi | 2011 | Chinese Geographical Science2011，21，1： | 21 |
| 3 | Preparation of carbon-coated iron oxide nanoparticles dispersed on graphene sheets and applications as advanced anode materials for lithium-ion batteries显示文摘 | Huilong Fei Zhiwei Peng Lei Li Yang Yang Wei Lu Errol L. G. Samuel Xiujun Fan James M. Tour | 2014 | Nano Research2014，7，4： | 10 |
| 4 | Rapid eutectic growth during free fall显示文摘Rapid eutectic growth of Sb-24%Cu alloy is realized in the drop tube during the free fall under the con-tainerless condition. Based on the analysis of crystal nuclea-tion and eutectic growth in the free fall condition, it is indicated that, with the increase of undercooling, microstruc-tural transition of Sb-24%Cu eutectic alloy proceeds from lamellar to anomalous eutectic structure. Undercoolings of 0 -154 K have been obtained in experiment. The maximum undercooling exceeds to 0.19TE. Calculated results exhibit that Cu2Sb compound is the primary nucleation phase, and that the primary Sb dendrite will grow more rapidly than the eutectic structure when undercooling is larger than 40 K. The eutectic coupled zone around Sb-24%Cu eutectic alloy leads strongly to the Cu-rich side and covers a composition range from 23.0% to 32.7%Sb. | Wenjing Yao Xiujun Han Bingbo Wei | 2002 | Chinese Science Bulletin2002，47，15： | 7 |
| 5 | Evolution of a mining induced fracture network in the overburden strata of an inclined coal seam显示文摘The geological conditions of the Pingdingshan coal mining group were used to construct a physical model used to study the distribution and evolution of mining induced cracks in the overburden strata.Digital graphics technology and fractal theory are introduced to characterize the distribution and growth of the mining induced fractures in the overburden strata of an inclined coal seam.A relationship between fractal dimension of the fracture network and the pressure in the overburden strata is suggested.Mining induced fractures spread dynamically to the mining face and up into the roof as the length of advance increases.Moreover,the fractal dimension of the fracture network increases with increased mining length,in general,but decreases during a period from overburden strata separation until the main roof collapses.It is a1so shown that overburden strata pressure plays an important role in the evolution of mining induced fractures and that the fractal dimension of the fractures increases with the pressure of the overburden. | Wei Xiujun Gao Mingzhong Lv Youchang Shi Xiangchao Gao Hailian Zhou Hongwei | 2012 | International Journal of Mining Science and Technology2012，22，6： | 7 |
| 6 | CircRNA-SORE mediates sorafenib resistance in hepatocellular carcinoma by stabilizing YBX1显示文摘Sorafenib is the first-line chemotherapeutic therapy for advanced hepatocellular carcinoma(HCC).However,sorafenib resistance significantly limits its therapeutic efficacy,and the mechanisms underlying resistance have not been fully clarified.Here we report that a circular RNA,circRNA-SORE(a circular RNA upregulated in sorafenib-resistant HCC cells),plays a significant role in sorafenib resistance in HCC.We found that circRNA-SORE is upregulated in sorafenib-resistant HCC cells and depletion of circRNA-SORE substantially increases the cell-killing ability of sorafenib.Further studies revealed that circRNA-SORE binds the master oncogenic protein YBX1 in the cytoplasm,which prevents YBX1 nuclear interaction with the E3 ubiquitin ligase PRP19 and thus blocks PRP19-mediated YBX1 degradation.Moreover,our in vitro and in vivo results suggest that circRNA-SORE is transported by exosomes to spread sorafenib resistance among HCC cells.Using different HCC mouse models,we demonstrated that silencing circRNA-SORE by injection of siRNA could substantially overcome sorafenib resistance.Our study provides a proof-of-concept demonstration for a potential strategy to overcome sorafenib resistance in HCC patients by targeting circRNA-SORE or YBX1. | Junjie Xu Lin Ji Yuelong Liang Zhe Wan Wei Zheng Xiaomin Song Kirill Gorshkov Qiming Sun Hui Lin Xueyong Zheng Jiang Chen Ren-an Jin Xiao Liang Xiujun Cai | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 7 | High undercooling and rapid dendritic growth of Cu-Sb alloy in drop tube显示文摘Droplets of Cu-20%Sb hypoeutectic alloy has been rapidly solidified in drop tube within the containerless condition. With the decrease of droplet diameter, undercooling increases and the microstructures of primary copper dendrite refines. Undercooling up to 207 K (0.17 TL) is obtained in experiment. Theoretic analysis indicated that, because of the broad temperature range of solidification, the rapid growth of primary copper dendrite is controlled by the solutal diffusion. Judging from the calculation of T0 curve in the phase diagram, it is shown that the critical undercooling of segregationless solidification is △T0=474 K. At the maximum undercooling of 207 K, the growth velocity of primary copper phase exceeds to 37 mm/s, and the distinct solute trapping occurs. | Yao Wenjing Xiujun Han Bingbo Wei | 2002 | Chinese Science Bulletin2002，47，15： | 3 |
| 8 | HSP70 alleviates sepsis-induced cardiomyopathy by attenuating mitochondrial dysfunction-initiated NLRP3 inflammasome-mediated pyroptosis in cardiomyocytes显示文摘Background:Sepsis-induced cardiomyopathy(SIC)is an identified serious complication of sepsis that is associated with adverse outcomes and high mortality.Heat shock proteins(HSPs)have been implicated in suppressing septic inflammation.The aim of this study was to investigate whether HSP70 can attenuate cellular mitochondrial dysfunction,exuberated inflammation and inflammasome-mediated pyroptosis for SIC intervention.Methods:Mice with cecal ligation plus perforation(CLP)and lipopolysaccharide(LPS)-treated H9C2 cardiomyocytes were used as models of SIC.The mouse survival rate,gross profile,cardiac function,pathological changes and mitochondrial function were observed by photography,echocardiography,hematoxylin-eosin staining and transmission electron microscopy.In addition,cell proliferation and the levels of cardiac troponin I(cTnI),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were determined by Cell Counting Kit-8,crystal violet staining and enzyme-linked immunosorbent assay.Moreover,mitochondrial membrane potential was assessed by immunofluorescence staining,and dynamin-related protein 1 and pyroptosis-related molecules[nucleotide-binding domain,leucine-rich-repeat containing family pyrin domain-containing 3(NLRP3),caspase-1,gasdermin-D(GSDMD),gasdermin-D N-terminal(GSDMD-N)]were measured by western blotting,immunoprecipitation and immunoblotting.Finally,hsp70.1 knockout mice with CLP were used to verify the effects of HSP70 on SIC and the underlying mechanism.Results:Models of SIC were successfully established,as reduced consciousness and activity with liparotrichia in CLP mice were observed,and the survival rate and cardiac ejection fraction(EF)were decreased;conversely,the levels of cTnI,TNF-αand IL-1βand myocardial tissue damage were increased in CLP mice.In addition,LPS stimulation resulted in a reduction in cell viability,mitochondrial destabilization and activation of NLRP3-mediated pyroptosis molecules in vitro.HSP70 treatment improved myocardial tissue damage,survival rate and cardiac dysfunction caused by CLP.Additionally,HSP70 intervention reversed LPS-induced mitochondrial destabiliza-tion,inhibited activation of the NLRP3 inflammasome,caspase-1,GSDMD and GSDMD-N,and decreased pyroptosis.Finally,knockout of hsp70.1 mice with CLP aggravated cardiac dysfunction and upregulated NLRP3 inflammasome activity,and exogenous HSP70 significantly rescued these changes.It was further confirmed that HSP70 plays a protective role in SIC by attenuating mitochondrial dysfunction and inactivating pyroptotic molecules.Conclusions:Our study demonstrated that mitochondrial destabilization and NLRP3 inflammasome activation-mediated pyroptosis are attributed to SIC.Interestingly,HSP70 ameliorates sepsis-induced myocardial dysfunction by improving mitochondrial dysfunction and inhibiting the acti-vation of NLRP3 inflammasome-mediated pyroptosis,and such a result may provide approaches for novel therapies for SIC. | Chenlu Song Yiqiu Zhang Qing Pei Li Zheng Meiyu Wang Youzhen Shi Shan Wu Wei Ni Xiujun Fu Yinbo Peng Wen Zhang Min Yao | 2022 | Burns & Trauma2022，10，1： | 2 |
| 9 | Genetically modified CD7-targeting allogeneic CAR-T cell therapy with enhanced efficacy for relapsed/refractory CD7-positive hematological malignancies:a phase I clinical study显示文摘Chimeric antigen receptor(CAR)-T cell therapy against T cell malignancies faces major challenges including fratricide between CAR-T cells and product contamination from the blasts.Allogeneic CAR-T cells,generated from healthy donor T cells,can provide ready-to-use,blast-free therapeutic products,but their application could be complicated by graft-versus-host disease(GvHD)and host rejection.Here we developed healthy donor-derived,CD7-targeting CAR-T cells(RD13-01)with genetic modifications to resist fratricide,GvHD and allogeneic rejection,as well as to potentiate antitumor function.A phase I clinical trial(NCT04538599)was conducted with twelve patients recruited(eleven with T cell leukemia/lymphoma,and one with CD7-expressing acute myeloid leukemia).All patients achieved pre-set end points and eleven proceeded to efficacy evaluation.No dose-limiting toxicity,GvHD,immune effector cell-associated neurotoxicity or severe cytokine release syndrome(grade≥3)were observed.28 days post infusion,81.8%of patients(9/11)showed objective responses and the complete response rate was 63.6%(7/11,including the patient with AML).3 of the responding patients were bridged to allogeneic hematopoietic stem cell transplantation.With a median follow-up of 10.5 months,4 patients remained in complete remission.Cytomegalovirus(CMV)and/or Epstein-Barr virus(EBV)reactivation was observed in several patients,and one died from EBV-associated diffuse large B-cell lymphoma(DLBCL).Expansion of CD7-negative normal T cells was detected post infusion.In summary,we present the first report of a Phase I clinical trial using healthy donor-derived CD7-targeting allogeneic CAR-T cells to treat CD7^(+)hematological malignancies.Our results demonstrated the encouraging safety and efficacy profiles of the RD13-01 allogeneic CAR-T cells for CD7^(+)tumors. | Yongxian Hu Yali Zhou Mingming Zhang Houli Zhao Guoqing Wei Wengang Ge Qu Cui Qitian Mu Gong Chen Lu Han Tingting Guo Jiazhen Cui Xiaoyan Jiang Xiujun Zheng Shuhui Yu Xiaolong Li Xingwang Zhang Mingxi Chen Xiuju Li Ming Gao Kang Wang Cheng Zu Hao Zhang Xiaohong He Yanbin Wang Dongrui Wang Jiangtao Ren He Huang | 2022 | Cell Research2022，32，11： | 2 |
| 10 | Characteristics of Channeling Flow in Cultivated Horizon of Saline Rice Soil显示文摘By applying bromide ion as tracer,the channeling flow has been quantitatively described in saline rice soil and alkaline soil of Da,an City,Jilin Province of China.Breakthrough curves of bromide ion in the saline rice soils after 1-year cultivation and 5-year cultivation and alkaline soil have been attained.Results show that the rice cultivation practice can improve the alkaline soil structure,however,it can accelerate the development of channeling flow pathway.Therefore,the channeling flow pathway has been developed widely in saline rice soil,but rarely in the alkaline soil.Three models of convection-dispersion equation(CDE),transfer functional model(TFM) and Back-Progation Network(BP Network) were used to simulate the transportation process of bromide ion.The peaks of probability density function of saline rice soil are higher with left skewed feature compared with that of the alkaline soil.It shows that the TFM and CDE can simulate the transportation process of the bromide ion in saline rice soil after 5-year cultivation,however,some deviation exists when it was used to simulate transportation process of bromide ion in saline rice soil after 1-year cul-tivation and alkaline soil;BP network can effectively simulate transportation process of bromide ion in both saline rice soil and alkaline soil. | LUO Jinming DENG Wei ZHANG Xiaoping YANG Fan LI Xiujun | 2006 | Chinese Geographical Science2006，16，4： | 1 |
| 11 | The Iidocaine in male patients with urinary retention following hemorrhoids operation painless urethral catheter application显示文摘 | Zhang Wei Guo Xiujun | | 0，，08： | 1 |
| 12 | Stapler vs suture closure of pancreatic remnant after distal pancreatectomy: a meta-analysis显示文摘 | Wei Zhou Ran Lv Xianfa Wang Yiping Mou Xiujun Cai Ingrid Herr | 2010 | The American Journal of Surgery2010，，4： | 1 |
| 13 | Genome assembly of the snow lotus species Saussurea involucrata provides insights into acacetin and rutin biosynthesis and tolerance to an alpine environment显示文摘Dear Editor,Understanding the evolution and survival mechanisms of endangered wild medicinal herbs is crucial for their cultivation,utilization,and conservation.The snow lotus species Saussurea involucrata(Kar.&Kir.)Sch.Bip.(2n=32)(i.e.the well-known Tianshan snow lotus)which belongs to the eudicot family Asteraceae,is a famous traditional Chinese medicinal herb having anti-inflammatory. | Yanxia Sun Aidi Zhang Jacob BLandis Wei Shi Xiujun Zhang Hang Sun Hengchang Wang | 2023 | Horticulture Research2023，10，10： | 0 |
| 14 | High-quality total RNA extraction from Magnolia sieboldii K.Koch seeds:a comparative evaluation显示文摘Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extraction. The extraction of high-quality RNA is important and can be a limiting factor in plant molecular biology experiments. Sufficient high-quality total RNA is required to elucidate the molecular regulation mechanism of germination. However, M. sieboldii seeds with large amounts of secondary metabolites also contain recalcitrant tissues for RNA isolation. We found two simple and low-cost RNA extraction methods for M. sieboldii seeds by evaluating and selecting eight types of methods and further optimizing these methods. The two methods were not only suitable for extracting M. sieboldii seed RNA but also applicable to RNAs from several other tissues. Total RNA extracted through these approaches was applicable for general molecular biology experiments such as qRT-PCR(quantitative real-time polymerase chain reactions). The protocols also meet the strict harsh requirements for transcriptome sequencing and small RNA sequencing. This study provides a powerful approach for future studies at the transcription level. | Jun Wei Xiaolin Zhang Zhe Hou Xiujun Lu | 2019 | Journal of Forestry Research2019，30，1： | 0 |
| 15 | CircRNA-SORE mediates sorafenib resistance inhepatocellular carcinoma by stabilizing YBX1显示文摘Sorafenib is the first-line chemotherapeutic therapy for advanced hepatocellular carcinoma(HCC).However,sorafenib resistance significantly limits its therapeutic efficacy,and the mechanisms underlying resistance have not been fully clarified.Here we report that a circular RNA,circRNA-SORE(a circular RNA upregulated in sorafenib-resistant HCC cells),plays a significant role in sorafenib resistance in HCC.We found that circRNA-SORE is upregulated in sorafenib-resistant HCC cells and depletion of circRNA-SORE substantially increases the cell-killing ability of sorafenib.Further studies revealed that circRNA-SORE binds the master oncogenic protein YBX1 in the cytoplasm,which prevents YBX1 nuclear interaction with the E3 ubiquitin ligase PRP19 and thus blocks PRP19-mediated YBX1 degradation.Moreover,our in vitro and in vivo results suggest that circRNA-SORE is transported by exosomes to spread sorafenib resistance among HCC cells.Using different HCC mouse models,we demonstrated that silencing circRNA-SORE by injection of siRNA could substantially overcome sorafenib resistance.Our study provides a proof-of-concept demonstration for a potential strategy to overcome sorafenib resistance in HCC patients by targeting circRNA-SORE or YBX1. | Junjie Xu Lin Ji Yuelong Liang Zhe Wan Wei Zheng Xiaomin Song Kirill Gorshkov Qiming Sun Hui Lin Xueyong Zheng Jiang Chen Ren-an Jin Xiao Liang Xiujun Cai | 2021 | Signal Transduction and Targeted Therapy2021，6，1： | 0 |
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