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| 1 | Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda显示文摘Edwardsiella tarda 是为 edwardsiellosis,淡水的严重传染疾病和海洋的鱼种类负责的病原体,并且当前认出是为人病原的种类。anti-idiotypic monoclonal 抗体(mAb ) , 1E11,被开发了。它模仿 E 的保护的 epitope。tarda 和罐头阻止鱼 E 的感染。tarda。在这研究,正确变量重( VH )并且可变的光( VL )基因被使用生物信息学方法从 1E11 获得,并且 15 氨基酸( Gly4Ser ) 3 连接器被用来为单个链变量碎片( scFv )的 VLlinkerVH 形式的构造一起保持二 V 域基因。然后, scFv 是进向量 pET-28a 的 subcloned,在 Escherichia coli BL21 房间表示了,并且由 SDSPAGE 并且西方的弄污识别了。称大约 50 g 的红鼓(Sciaenops ocellatus L.) 与不同 E 受到挑战。在种痘,死亡率和相对百分比幸存跟随的 4 个星期以后的 tarda 紧张被记录并且计算,并且在 scFv 亚群的鱼的幸存率显然比控制亚群的高(P < 0.01 ) 。连接酶的 immunosorbent 试金结果在 4 个星期以后显示出那种痘以后,在 scFv 组的鱼 sera 的特定的抗体的水平比控制组显著地高。这研究显示 recombinant 抗体 scFv 成功地被开发,并且它可以对 E 用作一个有效疫苗的候选人。tarda。 | Hong Qin Xiaohang Jin Weiquan Huang Yulin Liu | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 5 |
| 2 | Marsdenia tenacissima injection induces the apoptosis of prostate cancer by regulating the AKT/GSK3β/STAT3 signaling axis显示文摘Marsdenia tenacissima injection,a standard Marsdenia tenacissima extract(MTE),has been approved as an adjuvant therapeutic agent for various cancers.Our previous study showed that MTE inhibited the proliferation and metastasis of prostate cancer(PCa)cells.However,the underlying mechanisms and active ingredients of MTE against PCa were not completely understood.This study revealed that MTE induced significant decreases in cell viability and clonal growth in PCa cells.In addition,MTE induced the apoptosis of DU145 cells by reducing the mitochondrial membrane potential and increasing the expression of Cleaved Caspase 3/7,Cyt c,and Bax.In vivo,DU145 xenografted NOD-SCID mice treated with MTE showed significantly decreased tumor size.TUNEL staining and Western blot confirmed the pro-apoptotic effects of MTE.Network pharmacology analysis collected 196 ingredients of MTE linked to 655 potential targets,and 709 PCa-associated targets were retrieved,from which 149 overlapped targets were screened out.Pathway enrichment analysis showed that the HIF-1,PI3K-AKT,and ErbB signaling pathways were closely related to tumor apoptosis.Western blot results confirmed that MTE increased the expression of p-AKT^(Ser473) and p-GSK3β^(Ser9),and decreased the expression of p-STAT3^(Tyr705) in vitro and in vivo.A total of 13 compounds in MTE were identified by HPLC-CAD-QTOF-MS/MS and UPLCQTOF-MS/MS.Molecular docking analysis indicated that six compounds may interact with AKT,GSK3β,and STAT3.In conclusion,MTE induces the endogenous mitochondrial apoptosis of PCa by regulating the AKT/GSK3β/STAT3 signaling axis,resulting in inhibition of PCa growth in vitro and in vivo. | LI Xiaolan HE Songhua LIANG Wei ZHANG Weiquan CHEN Xin LI Qiaofeng YANG Xin LIU Yanying ZHU Dan LI Li LIU Buming SU Zhiheng CHEN Jie GUO Hongwei | 2023 | Chinese Journal of Natural Medicines2023，21，2： | 2 |
| 3 | CALDER6N-ZYGMUND OPERAORS ON THE HARDY SPACES OF WEIGHTED HERZ TYPE显示文摘Applying the decomposition theorems in [1] and [2] , we obtain the boundedness theorem of Calderbn-Zygmund operator of type 6 on the Hardy spaces of weighted Herz type and establish interpolation theorem of linear operators on the weighted Herz spaces. - | Liu Weiquan and Lu Shanzhen(Beijing Normal University, China) | 1997 | Analysis in Theory and Applications1997，13，2： | 2 |
| 4 | Laser Cooling and Deceleration of Neutral Atoms by Red-Shifted Diffuse Light显示文摘We report a cooling and deceleration experiment of a thermal beam by using a nearly resonant red-shifted diffuse light in an optical integral sphere cavity.By using of this red-shifted diffuse light,the velocity of a part of thermal sodium atoms is cooled to 380m/s and the velocity width of cooled atoms is about 18m/s.The mechanism of this kind of laser cooling and the experimental results are discussed. | CHEN Hongxin CAI Weiquan LIU Liang SHU Wei LI Fosheng WANG Yuzhu | 1994 | Chinese Physics Letters1994，11，9： | 1 |
| 5 | FOXP3 Is an X-Linked Breast Cancer Suppressor Gene and an Important Repressor of the HER-2/ErbB2 Oncogene显示文摘 | Tao Zuo Lizhong Wang Carl Morrison Xing Chang Huiming Zhang Weiquan Li Yan Liu Yin Wang Xingluo Liu Michael W.Y. Chan Jin-Qing Liu Richard Love Chang-gong Liu Virginia Godfrey Rulong Shen Tim H.-M. Huang Tianyu Yang Bae Keun Park Cun-Yu Wang Pan Zheng Yan | 2007 | Cell2007，，7： | 1 |
| 6 | FOXP3 Is an X-Linked Breast Cancer Suppressor Gene and an Important Repressor of the HER-2/ErbB2 Oncogene显示文摘 | Tao Zuo Lizhong Wang Carl Morrison Xing Chang Huiming Zhang Weiquan Li Yan Liu Yin Wang Xingluo Liu Michael W.Y. Chan Jin-Qing Liu Richard Love Chang-gong Liu Virginia Godfrey Rulong Shen Tim H.-M. Huang Tianyu Yang Bae Keun Park Cun-Yu Wang Pan Zheng Yan | 2007 | Cell2007，，7： | 1 |
| 7 | Build-in Reliability Analysis for Circuit Design in the Nanometer Technology Era 显示文摘 | Liu Zhihong Zhang Weiquan Mu Fuchen | 2004 | IEEE International Conference on Integrated Circuit Design and Technology2004，，： | 1 |
| 8 | Detection of CD2 expression in chicken hematogenic embryo yolk sac lymphoid cells prior to thymus genesis显示文摘从在舞台 16 点的小鸡胚胎的淋巴的单音的原子房间在胎儿的肝和胸腺开始以前被收集学习造血的蛋黄囊的区别和功能并且检测 CD2 是否发生在淋巴的单音的原子房间的表面上。显型和房间表面蛋白质 E 受体的功能的活动和胚胎的 E + 房间的超微结构与成熟 T 房间的那些相比。我们的结果显示在胚胎的淋巴细胞和成熟 T 房间的 E 受体之间的 99.36% 相同。另外的类似包括分子的分发,动机,形成一个红血球插座,在胚胎的淋巴细胞之间被检测并且成熟的能力,受体 ligand 建筑群的结构,和信号隧道的符合构造表示 CD2 T 房间。这些结果显示 CD2 已经在胎儿的肝和胸腺开始以前被表示并且它的表示不依赖于 thymic 微型环境。 | Dongyu Zhou Jigui Wang Weiquan Liu Rongxiu Liu Yuehu Pei | 2008 | Progress in Natural Science:Materials International2008，18，12： | 1 |
| 9 | Effects of clinorotation on COL-1A1-EGFP gene expression显示文摘 | DAI Zhongquan LI Yinghui DING Bai ZHANG Yuguo LIU Weiquan LIU Pengpeng | 2005 | 中国生物学文摘2005，19，7： | 0 |
| 10 | Atomic Motion in a Misaligned Standing-Wave Field显示文摘Theoretical and experimental results of laser cooling and laser heating of an atomic beam in a standing-wave field and in a misaligned standing-wave field are reported.The results show that in strong fields,laser heating in a standing-wave field become laser cooling in a misaligned standing-wave field and in weak fields,laser cooling become more efficient in a standing-wave field than in a misaligned standing-wave field. | WANG Yuzhu LIU Liang CAI Weiquan CHENG Yudan | 1991 | Chinese Physics Letters1991，8，7： | 0 |
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