|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Small Interfering RNA-mediated Caveolin-1 Knockout on Plasminogen Activator Inhibitor-1 Expression in Insulin-stimulated Human Vascular Endothelial Cells显示文摘把人的脉管的 endothelial 房间(ECV304 ) 用作目标,我们学习了 caveolin (CAV ) 的效果在 plasminogen 的刺激胰岛素的表示期间的 -1 禁止者(白族)-1。代表 RNAi CAV-1 基因的适当搁浅单人赛的 oligonucleotides 被 Ambion 软件分析。在退火产生双 stranded oligonucleotides (dsoligo ) 以后,它被克隆进由 T4 DNA 连接酶包含 RNA 聚合酶 III 表示元素的 pENTR/U6 入口向量。从 pENTR/U6 入口转移的短发卡(shRNA ) 序列与 LR 再结合反应被克隆进 pLenti6/BLOCK-iT-DEST 向量。由定序的 Afteridentification,我们成功地用网关技术构造了 CAV-1 RNAi lentiviral 表达式系统。Silencing 效率是由即时 reversetranscription 聚合酶链反应,免疫荧光染色并且西方的弄污的 assayed。ECV304cells 在包含胰岛素的不同集中的媒介是有教养的(1x10 ^( 有 CAV-1 基因 silenced 的 -9)to1x10^(-7) M ) 。表示水平和潜水艇 PAI-1 和 CAV-1 的细胞的本地化用反向的抄写聚合酶链反应,免疫荧光染色和蛋白质印迹试金被比较。结果证明有势力抑制 ofCAV-1 表示能到达 85% ,并且它对 CAV-1-derived shRNA,不是导出 theS100A13 的 shRNA 特定。在蛋白质确实在房间膜下面积累了的在与生理的胰岛素,而是 PAI-1 孵化的 RNAi^+andRNAi^- ECV304 房间之间的 PAI-1 表示没有戏剧的差别。当胰岛素的集中增加了, PAI-1 的表示是起来调整的,而 CAV-1 的表示稀释了。而且, PAI-1 清楚地在 CAV-1knockdown 以后扩充了。这些结果显示 hyperinsulinism 能由 inhibitingCAV-1 支持 PAI-1 表示,并且稳定或在 endothelial 房间的起来调整的 CAV-1 表示可能在糖尿病减少大容器和毛状的容器的复杂并发症。 | Huiling YANG Shuya HE Zhihua QUAN Weixia PENG Bin YAN Jianghua LIU Fang WEN Renxian CAO Yangyan XU Gebo WEN Weixin HU | 2007 | Acta Biochimica et Biophysica Sinica2007，39，3： | 7 |
| 2 | Effects of Calcination Temperature of Boron-Containing Magnesium Oxide Raw Materials on Properties of Magnesium Phosphate Cement as a Biomaterial显示文摘A new magnesium phosphate bone cement(MPBC) was prepared as a byproduct of boroncontaining magnesium oxide(B-MgO) after extracting Li_2CO_3 from salt lakes. We analyzed the elementary composition of the B-MgO raw materials and the effects of calcination temperature on the performance of MPBC. The phase composition and microstructure of the B-MgO raw materials and the hydration products(KMg PO_4·6H_2O) of MPBC were analyzed by X-ray diffraction and scanning electron microscopy. The results showed that ionic impurities and the levels of toxic elements were sufficiently low in B-MgO raw materials to meet the medical requirements for Mg O(Chinese Pharmacopeia, 2010 Edition) and for hydroxyapatite surgical implants(GB23101.1-2008). The temperature of B-Mg O calcination had a marked influence on the hydration and hardening of MPBC pastes. Increasing calcination temperature prolonged the time required for the MPBC slurry to set, significantly decreased the hydration temperature, and prolonged the time required to reach the highest hydration temperature. However, the compressive strength of hardened MPBC did not increase with higher calcination temperatures. In the 900-1 000 ℃ temperature range, the hardened MPBC had a higher compressive strength. Imaging analysis suggested that the setting time and the highest hydration temperature of MPBC pastes were dependent on the size and crystal morphology of the B-Mg O materials. The production and microstructure compactness of KMgPO_4·6H_2O, the main hydration product, determined the compressive strength. | 董金美 余红发 XIAO Xueying LI Ying WU Chengyou WEN Jing TAN Yongshan CHANG Chenggong ZHENG Weixin | 2016 | Journal of Wuhan University of Technology(Materials Science)2016，31，3： | 2 |
| 3 | Synthesis of Cu (OH)2 and CuO nanoribbon arrays on a copper surface 显示文摘 | Wen Xiaogang Zhang Weixin Yang Shihe | 2003 | Langmuir2003，19，： | 1 |
| 4 | Nanorods of manganese oxides: Synthesis, characterization and catalytic application显示文摘 | Zeheng Yang Yuancheng Zhang Weixin Zhang Xue Wang Yitai Qian Xiaogang Wen Shihe Yang | 2005 | Journal of Solid State Chemistry2005，，3： | 1 |
| 5 | The effect of solid diffusion surface alloying on properties of ZM5 magnesium alloy 显示文摘 | Ma Youping Xu Kewei Wen Weixin | 2005 | Surface & Coatings Technology2005，190，23： | 1 |
| 6 | Large-scale synthesis of β-MnO 2 nanorods and their rapid and efficient catalytic oxidation of methylene blue dye显示文摘 | Weixin Zhang Zeheng Yang Xue Wang Yuancheng Zhang Xiaogang Wen Shihe Yang | 2005 | Catalysis Communications2005，，6： | 1 |
| 7 | Pho- toelectrochemistry of pure and core/sheath nanowire arrays of Cu2S directly grown on copper electrodes显示文摘 | Yang Shangfeng Wen Xiaogang Zhang Weixin | 2005 | J Electro- them SOe2005，152，3： | 1 |
| 8 | Synthesis of Cu(OH)2 and CuO nanoribbon arrays on a copper surface 显示文摘 | Wen Xiaogang Zhang Weixin Yang Shihe | 2003 | Langmuir2003，19，： | 1 |
| 9 | Solution Phase Synthesis of Cu (OH)2 Nanoribbons by Coordination SelfAssembly Using Cu2S Nanowires as Precursors显示文摘 | Xiaogang Wen Weixin Zhang Shihe Yang | 2002 | NANO Letters2002，12，2： | 1 |
| 10 | Synthesis of Cu (OH)2 and CuO Nanoribbon Arrays on a Copper surface显示文摘 | Xiaogang Wen Weixin Zhang Shihe Yang | 2003 | Langmuir2003，19，： | 1 |
| 11 | Tetramethylpyrazine improves the recovery of spinal cord injury via Akt/Nrf2/HO-1 pathway显示文摘 | Chao Wang Peng Wang Wen Zeng Weixin Li | 2016 | Bioorganic & Medicinal Chemistry Letters2016，，： | 1 |
| 12 | Extraction of straight field roads between farmlands based on agricultural vehicle-mounted LiDAR显示文摘The application of autonomous agricultural vehicles is gaining popularity as a way to increase production efficiency and lower operational costs.To achieve high performance,perception tasks(such as obstacle detection,road extraction,and drivable area extraction)are of great importance.Compared with structured roads,field roads between farmlands,including unstructured roads and semi-structured roads,are unfavorable for autonomous agricultural vehicle driving due to their bumpiness and unstructured nature.This study proposed an extraction method for the straight field roads between farmlands.The proposed method was based on the point cloud data acquired by LiDAR(Velodyne VLP-16)mounted on a John Deere 12046B-1204 tractor.The proposed method has three aspects:Euclidean Clustering-based extraction,boundary-based extraction,and road point cloud curve segment modification.Firstly,Euclidean Clustering with K-Dimensional(KD)-Tree data structure was adopted to extract the road curve segments close to the LiDAR composed of road points.Secondly,the boundary lines constraint was constructed to extract the distant road curve segments.Thirdly,the local distance ratio was used to modify the extracted road curve segments.The average extraction accuracy for both semi-structured and unstructured roads exceeded 98%,and the false positive rate(FPR)was less than 0.5%.These experimental findings demonstrated that the proposed road extraction method was precise and effective.The proposed method of this study can be applied to enhance the perception ability of autonomous agricultural vehicles thereby increasing the efficiency and safety of field road driving. | Lili Yang Yuanyuan Xu Yajie Liang Jia Qin Yuanbo Li Xinxin Wang Weixin Zhai Long Wen Zhibo Chen Caicong Wu | 2022 | International Journal of Agricultural and Biological Engineering2022，15，5： | 1 |
| 13 | Synthesis of Cu(OH)2and Cu O Nanoribbon Arrays on a Copper Surface显示文摘 | Wen Xiaogang Zhang Weixin Yang Shihe | 2003 | American Chemical Society2003，19，： | 1 |
| 14 | Synthesis and characterization of uniform arrays of copper sulfide nanorods coated with nanolayers of polypyrrole显示文摘 | Weixin Zhang W X Wen X G etc | 2003 | Langmuir2003，19，： | 1 |
| 15 | Long-term Properties of Aeolian Sand-magnesium Oxychloride Cement Composites and Its Engineering Application显示文摘In order to prepare a new material with long-term stable performance,low cost,easy construction,and ecological environmental protection,the influence of aeolian sand on the compressive and flexural strength as well as micro morphology and phase composition of magnesium oxychloride cement(MOC)was studied.The experimental results indicate that,with the increase of content of doping sand,the compressive strength and flexural strength of MOC decrease significantly.However,when the quality ratio of aeolian sand and light burned magnesia powder is 1:8,the performance meets the actual engineering needs.Namely,the compressive strength of MOC is not less than 18 MPa,and flexural strength is not less than 4 MPa.Meanwhile,within 12 months of age,the compressive strength and flexural strength are stable.There is no obvious change in phase composition,and its main phase is still 5·1·8 phase.Microscopic appearance changes from needle-like to gel-like shape.Based on engineering applications,it is found that when the novel sand-fixing material is used in the field for one year,its macroscopic feature is not damaged,compressive strength and flexural strength are also more stable,phase composition negligibly changes,and micro morphology has also been turned into be gellike shape.These further confirm the long-term stability and weather resistance of MOC doping aeolian sand,providing theoretical and technical support for the widely application of MOC in the field of sand fixation in the future. | 常成功 DONG Jinmei ZHENG Weixin WEN Jing 阎峰云 肖学英 AN Lingyun | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，4： | 0 |
| 16 | Use of serum Golgi protein 73 for screening chronic hepatitis B virus infection patients needing antiviral therapy in the community显示文摘Chronic hepatitis B virus (HBV) infection is a major global public health problem. Approximately 887,000 people die of HBV infection-related diseases annually, with cirrhosis and hepatocellular carcinoma (HCC) being the principal causes of mortality.[1] Timely antiviral therapy greatly reduces the risks of cirrhosis and HCC. However, unfortunately, of those patients who are eligible for antiviral treatment, only 25% of patients in clinic settings and 12% of those in community settings obtain timely antiviral therapy.[2] Therefore, reliable means of identifying patients with chronic HBV infection that require antiviral therapy are necessary, particularly for use in the community. | Jinwei Duan Xiajie Wen Huai Wang Weixin Chen Pei Gao Qianli Yuan Han Zheng Yanna Liu Jiang Wu Jie Wang Mingjie Yao Fengmin Lu | 2022 | Chinese Medical Journal2022，135，22： | 0 |
| 17 | Behavior recognition and fuel consumption prediction of tractor sowing operations using smartphone显示文摘In order to qualitatively recognize the behaviors and investigate the relationship between fuel consumption and machinery driving modes of the tractor in a low-cost approach,this study proposed a method for behavior recognition and fuel consumption prediction of tractor sowing operations using a smartphone.First,three driving modes were developed for maize sowing scenarios:manual driving assisted driving and unmanned driving.While sowing,smartphone software and CAN(Controller Area Network)storage devices collected both positional data and engine operating conditions.Second,the tractor trajectory points were divided into kinematic sequences,with six driving cycle indicators built in each series based on the time window.Based on the semantic information of the kinematic sequences,the three operations of sowing,seeds filling,and turning round were well recognized.Last,a model for maize sowing fuel consumption forecast was advanced using the principal component analyses and random forest algorithm,regarding three factors:driving cycles,operating behaviors,and driving patterns.When compared to the traditional K-means algorithm,the results demonstrated that the harmonic mean of the precision and recall(F1 score)of sowing behavior recognition,seeds filling behavior recognition,and turning behavior recognition were enhanced by 2.06%,8.99%,and 21.79%,respectively.In terms of the impacts of driving modes and operating behaviors on fuel consumption,assisted driving mode had the lowest fuel usage for both sowing and turning behavior.Therefore,assisted driving is the most fuel-efficient mode for maize sowing.Combining the three driving modes,the relative error of the fuel consumption prediction model was 0.11 L/h,with the manual driving mode having the lowest relative error at 0.09 L/h.This research method lays the foundation for the optimization of tractor operation behavior,the selection of tractor driving mode,and the fine management of tractor fuel consumption. | Lili Yang Weize Tian Weixin Zhai Xinxin Wang Zhibo Chen Long Wen Yuanyuan Xu Caicong Wu | 2022 | International Journal of Agricultural and Biological Engineering2022，15，4： | 0 |
| 18 | Morphology-Dependent Catalysis of CeO_(2)-Based Nanocrystal Model Catalysts显示文摘Comprehensive Summary Recent progress in colloidal synthesis has realized the preparations of uniform nanocrystal(NC)model catalysts with rich and well-controlled morphologies that were employed to explore structure-activity relationships of powder catalysts,similar to single-crystal-based model catalysts under ultrahigh vacuum conditions but can work at the same conditions as powder catalysts without the'materials gap'and'pressure gap'.In this perspective,the CeO_(2)-based NC model catalysts with various morphologies are included and their relevant progresses are critically reviewed.The detailed descriptions of morphology-controlled synthesis and characterizations of uniform CeO_(2)NCs. | Yang Wen Qiuyu Huang Zhenhua Zhang Weixin Huang | 2022 | Chinese Journal of Chemistry2022，40，15： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员