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| 1 | Purification of total DNA extracted from activated sludge显示文摘从活性污泥样品提取的全部的脱氧核糖核酸的纯化被学习。从活性污泥提取的全部的脱氧核糖核酸的产量和纯净上的抽取缓冲区和细胞溶解处理(溶解酵素,钠 dodecyl 硫酸盐(SDS ) , sonication,机械工厂和热吃惊) 的效果被调查。SDS 和机械工厂是为房间细胞溶解的最有效的方法,这被发现,并且,两个都由 SDS 和热吃惊给了最高的脱氧核糖核酸收益,最纯的脱氧核糖核酸摘录能被获得。有另外的细胞溶解处理的 SDS 的联合例如 sonication 和热吃惊,能显然增加脱氧核糖核酸收益而且导致严重的砍。为粗略的脱氧核糖核酸摘录的纯化,聚乙烯化合物的 polypyrrolidone 被用于腐殖的沾染物的移动。Cetyltrimethyl 铵溴化物,钾醋酸盐和酚 / 氯仿被用来把蛋白质和多糖从粗略的脱氧核糖核酸移开。粗略的脱氧核糖核酸被 isopropanol 降水进一步净化。因此,一个合适的协议为脱氧核糖核酸抽取被建议,产出大约 49.9 mg (全部的脱氧核糖核酸)/g 不稳定的推迟的固体,和脱氧核糖核酸摘录成功地为 16S rDNA 和 16S rDNA V3 区域在 PCR 扩大被使用。16S rDNA V3 区域的 PCR 产品允许 DGGE 分析(使中毒的坡度胶化电气泳动) 可能。 | SHAN Guobin JIN Wenbiao Edward KHLAM XING Xinhui | 2008 | Journal of Environmental Sciences2008，20，1： | 8 |
| 2 | Mechanism on minimization of excess sludge in oxic-settling- anaerobic (OSA) process显示文摘The oxic-settling-anaerobic(OSA)process is a promising wastewater treatment technique for efficiently reducing sludge production and improving the stability of process operation.In this paper,the possible factors of sludge reduction such as sludge decay,uncoupled metabol-ism,and anaerobic oxidation with low sludge production were discussed in the OSA process.It has been confirmed that sludge decay is the decisive cause in the OSA process,accounting for 66.7%of sludge production reduction.Sludge decay includes hydrolysis and acidogenesis of dead microorganisms and particle organic carbon adsorbed in sludge floc and endogenous metabolism.By batch experi-ments,it has been proven that there is energetic uncoupling in the OSA system since microorganisms were exposed to alternative anaerobic and aerobic environment.It accounts for about 7.5%of sludge production reduction.Soluble chemical oxygen demand(SCOD)released from the anaerobic sludge tank in the OSA process was used as the substrate for cryptic growth.The substrate was used for anoxic denitrifying,anaerobic phosphorus release,sulfate reduction,and methane production.These anaerobic reactions in the sludge anaerobic tank have lower sludge production than in the aerobic oxidation when equivalent SCOD is consumed,which may lead to approximately 23%of sludge reduction in the OSA process.It has been concluded that multiple causes resulted in the minimization of excess sludge in the OSA system.The microbial community structure and diversity of sludge samples from the CAS(conventional activated sludge)and OSA systems were investigated by 16 SrDNA PCR-DG-DGGE(poly-merase chain reaction-double gradient-denaturing gradient gel electrophoresis).DGGE profile and cluster analysis showed more abundant species in the OSA system contrasting to microbial communities in the CAS system. | Jianfang WANG Qingliang ZHAO Wenbiao JIN Jikan LIN | 2008 | Frontiers of Environmental Science & Engineering2008，2，1： | 3 |
| 3 | Abundance and diversity of ammonia-oxidizing archaea in response to various habitats in Pearl River Delta of China, a subtropical maritime zone显示文摘Ammonia-oxidizing archaea(AOA) are widely considered key to ammonia oxidation in various environments.However,little work has been conducted to simultaneously investigate the abundance and diversity of AOA as well as correlations between archaeal amoA genotypes and environmental parameters of different ecosystems at one district.To understand the abundance,diversity,and distribution of AOA in Pearl River Delta of China in response to various habitats,the archaeal amoA genes in soil,marine,river,lake,hot spring and wastewater treatment plant(WWTP) samples were investigated using real-time fluorescent quantitative PCR and clone libraries.Our analyses indicated that the diversity of AOA in various habitats was different and could be clustered into five major clades,i.e.,estuary sediment,marine water/sediment,soil,hot spring and Cluster 1.Phylogenetic analyses revealed that the structure of AOA communities in similar ecological habitats exhibited strong relation.The canonical correspondence method indicated that the AOA community structure was strongly correlated to temperature,pH,total organic carbon,total nitrogen and dissolved oxygen variables.Assessing AOA amoA gene copy numbers,ranging from 6.84 × 106 to 9.45 × 107 copies/g in dry soil/sediment,and 6.06 × 106 to 2.41 × 107 copies/L in water samples,were higher than ammonia-oxidizing bacteria(AOB) by 1-2 orders of magnitude.However,AOA amoA copy numbers were much lower than AOB in WWTP activated sludge samples.Overall,these studies suggested that AOA may be a major contributor to ammonia oxidation in natural habitats but play a minor role in highly aerated activated sludge.The result also showed the ratio of AOA to AOB amoA gene abundance was positively correlated with temperature and less correlated with other environmental parameters.New data from our study provide increasing evidence for the relative abundance and diversity of ammonia-oxidizing archaea in the global nitrogen cycle. | Zhixin Li Wenbiao Jin Zhaoyun Liang Yangyang Yue Junhong Lv | 2013 | Journal of Environmental Sciences2013，25，6： | 3 |
| 4 | Rock fracturing tracing by image processing显示文摘 | Wang Weixing Jin Wenbiao Huang Xing | 2004 | Journal of Fndan university(Natund Science)2004，43，5： | 1 |
| 5 | Bioaugmentation of a biological contact oxidation ditch with indigenous nitrifying bacteria for in situ remediation of nitrogen-rich stream water显示文摘 | Yan Jiao Qingliang Zhao Wenbiao Jin Xiaodi Hao Shijie You | 2010 | Bioresource Technology2010，，2： | 1 |
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