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| 1 | Protective effects of nitric oxide on salt stress-induced oxidative damage to wheat (Triticum aestivum L.)leaves显示文摘The changes of chlorophyll and malondialde-hyde (MDA) contents, plasma membrane permeability confirmed that 0.1 and 1 mmol/L sodium nitroprusside (SNP), a donor of nitric oxide (NO) in vivo, could markedly alleviate the oxidative damage to wheat (Triticum aestivum L.) leaves induced by 150 and 300 mmol/L NaCl treatments, respectively. Further results proved that NO significantly enhanced the activities of superoxide dismutase (SOD) and catalase (CAT), both of which separately contributed to the delay of O2- and H2O2 accumulation in wheat leaves under saltstress. Meanwhile, the accumulation of proline was apparently accelerated. Therefore, these results suggested that NO could strongly protect wheat leaves from oxidative damage caused by salt stress. | Haihua Ruan Wenbiao Shen Maobing Ye Langlai Xu | 2002 | Chinese Science Bulletin2002,47,8: | 37 |
| 2 | Carbon monoxide-induced adventitious rooting of hypo-cotyl cuttings from mung bean seedling显示文摘In this paper, the regulatory role of ex-ogenous carbon monoxide (CO) during the genera-tion and development of adventitious roots in mung bean (Phaseolus radiatus L. cv. Mingguang) hypo-cotyl cuttings was surveyed. The results indicated that, like nitric oxide (NO), CO donor Hematin in-duced adventitious rooting in dose- and time-de- pendent manners. These responses were also prov- en by the addition of gaseous CO aqueous solution with different concentrations. Additionally, the above CO-mediated effect was differently reversed when CO scavenger hemoglobin (Hb), CO specific syn-thetic inhibitor ZnPPIX, the auxin transport inhibitor NPA and nitric oxide synthase (NOS) inhibitor L-NAME were added with Hematin, respectively. Furthermore, after being treated with CO for 36 h, NO fluorescence was significantly enhanced and mainly distributed in the zone of vascular bundles whereas the specific NO scavenger cPTIO significantly inhib-ited NO fluorescence induced by CO, indicating that CO induces adventitious rooting of hypocotyl cuttings from mung bean seedling probably mediated by NO/NOS pathway. | XU Ji XUAN Wei HUANG Benkai ZHOU Yehua LING Tengfang XU Sheng SHEN Wenbiao | 2006 | Chinese Science Bulletin2006,51,6: | 16 |
| 3 | Reservoir Characteristics of Lacustrine Shale and Marine Shale:Examples from the Songliao Basin,Bohai Bay Basin and Qiannan Depression显示文摘Lacustrine shale from the Qingshankou Formatin of Songliao basin and the Shahejie Formation of Bohai Bay basin, and marine shale from the lower Cambrian Jiumenchong Formation of Qiannan depression were analysed by using rock pyrolysis, TOC(total organic carbon), XRD(X-ray diffraction), SEM(scanning electron microscope), FE-SEM(field emission scanning electron microscope), high pressure mercury intrusion, and low pressure N_2 and CO_2 gas adsorption experiments, in aim to reveal their reservoir features. The results show that:(1) the width of micro-pores of all the studied samples mainly ranges from 0.45 to 0.7 nm indicated by CO_2 isotherms, and the width of meso-pores is less than 10 nm, with type IV adsorption isotherms and type H2 hysteresis loop, indicative of 'ink-bottle'-shaped pores. Good correlations exist among pore volume, surface area and averaged pore diameter, and a good positive correlation exists between micro-pore volume and TOC content; however, there is no obvious correlation between meso-pore volume and TOC content;(2) interparticle pores, pores among the edge of mineral grains and organic matter pores were all identified in marine and lacustrine shale, among which the interparticle pores may be influence by dissolution effect. Not all bitumen develops organic matter pore, and only high to over mature bitumen present pores. Now the description methods of micrometer scale pores developed in shale are very lack. Microfractures developed in Jiyang depression and dissolution interparticle pores developed in Songliao Basin should be the accumulation sites for shale oil in lacustrine shale, and can be as sweet spots. | WANG Min LU Shuangfang WANG Zhiwei LIU Yang HUANG Wenbiao CHEN Fangwen XU Xingyou LI Zheng LI Jijun | 2016 | Acta Geologica Sinica(English Edition)2016,90,3: | 10 |
| 4 | Purification of total DNA extracted from activated sludge显示文摘从活性污泥样品提取的全部的脱氧核糖核酸的纯化被学习。从活性污泥提取的全部的脱氧核糖核酸的产量和纯净上的抽取缓冲区和细胞溶解处理(溶解酵素,钠 dodecyl 硫酸盐(SDS ) , sonication,机械工厂和热吃惊) 的效果被调查。SDS 和机械工厂是为房间细胞溶解的最有效的方法,这被发现,并且,两个都由 SDS 和热吃惊给了最高的脱氧核糖核酸收益,最纯的脱氧核糖核酸摘录能被获得。有另外的细胞溶解处理的 SDS 的联合例如 sonication 和热吃惊,能显然增加脱氧核糖核酸收益而且导致严重的砍。为粗略的脱氧核糖核酸摘录的纯化,聚乙烯化合物的 polypyrrolidone 被用于腐殖的沾染物的移动。Cetyltrimethyl 铵溴化物,钾醋酸盐和酚 / 氯仿被用来把蛋白质和多糖从粗略的脱氧核糖核酸移开。粗略的脱氧核糖核酸被 isopropanol 降水进一步净化。因此,一个合适的协议为脱氧核糖核酸抽取被建议,产出大约 49.9 mg (全部的脱氧核糖核酸)/g 不稳定的推迟的固体,和脱氧核糖核酸摘录成功地为 16S rDNA 和 16S rDNA V3 区域在 PCR 扩大被使用。16S rDNA V3 区域的 PCR 产品允许 DGGE 分析(使中毒的坡度胶化电气泳动) 可能。 | SHAN Guobin JIN Wenbiao Edward KHLAM XING Xinhui | 2008 | Journal of Environmental Sciences2008,20,1: | 8 |
| 5 | Hydrogen sulfide, a signaling molecule in plant stress responses显示文摘Gaseous molecules, such as hydrogen sulfide(H_2S)and nitric oxide(NO), are crucial players in cellular and(patho)physiological processes in biological systems. The biological functions of these gaseous molecules, which were first discovered and identified as gasotransmitters in animals, have received unprecedented attention from plant scientists in recent decades. Researchers have arrived at the consensus that H_2S is synthesized endogenously and serves as a signaling molecule throughout the plant life cycle.However, the mechanisms of H_2S action in redox biology is still largely unexplored. This review highlights what we currently know about the characteristics and biosynthesis of H_2S in plants. Additionally,we summarize the role of H_2S in plant resistance to abiotic stress. Moreover, we propose and discuss possible redox-dependent mechanisms by which H_2S regulates plant physiology. | Jing Zhang Mingjian Zhou Heng Zhou Didi Zhao Cecilia Gotor Luis CRomero Jie Shen Zhenglin Ge Zhirong Zhang Wenbiao Shen Xingxing Yuan Yanjie Xie | 2021 | Journal of Integrative Plant Biology2021,63,1: | 8 |
| 6 | Ant Colony Clustering Algorithm for Underdetermined BSS显示文摘 | HE Xuansen WANG Fang CHAI Wenbiao WU Liangmin | 2013 | Chinese Journal of Electronics2013,22,2: | 7 |
| 7 | Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles显示文摘The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria. | Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li | 2015 | Science China Chemistry2015,58,11: | 5 |
| 8 | Scale-Dependent Nature of Porosity and Pore Size Distribution in Lacustrine Shales: An Investigation by BIB-SEM and X-Ray CT Methods显示文摘Due to heterogeneous pore distributions within shales,petrophysical properties of shales determined by scanning electron microscopy(SEM) and X-ray computed tomography(CT) methods strongly depend on the observed domain size(analysis scale). In this paper,the influence of the analysis scale on areal and bulk porosities and pore size distribution(PSD) for lacustrine shales from the Dongying sag of Bohai Bay Basin,China were investigated using broad ion beam(BIB)-SEM and X-ray CT methods.The BIB-SEM cross-sections with high imaging resolution(10 nm/pixel) and a large field of view(>1 mm2)mainly describe the 2 D nanoscale pore system in the two shales(samples F41#-2 and Y556#-1),while CTbased 3 D reconstructions with resolutions of 0.42(F41#-1) and 0.5 μm/pixel(H172#-1) reflect the 3 D submicron pore system. The results indicate that the areal(bulk) porosity exhibits a multiple power-law distribution with increasing analysis area(volume),which can be used to extrapolate the porosity of a given area(volume). Based on SEM and CT investigations,the sizes of the minimum representative elementary areas(REAs) and volumes(REVs) were determined respectively,which are closely associated with the heterogeneousness of the pore system. Minimum REAs are proposed to be 2.93×10~4(F41#-2) and 0.91×10~4μm2(Y556#-1),and minimum REVs are 0.016(F41#-1) and 0.027 mm^3(H172#-1). As the analyzed areas(volumes) are larger than the minimum REA(REV),obtained 2 D(3 D) PSDs are comparable to each other and can be considered to reflect the shale PSD. These results provide insights into the porosity and PSD characterization of shales by SEM and X-ray CT methods. | Junqian Li Pengfei Zhang Shuangfang Lu Chen Chen Haitao Xue Siyuan Wang Wenbiao Li | 2019 | Journal of Earth Science2019,30,4: | 5 |
| 9 | Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis显示文摘Hydrogen sulfide(H2S)is a signaling molecule that regulates plant hormone and stress responses.The phytohormone abscisic acid(ABA)plays an important role in plant adaptation to unfavorable environmental conditions and induces the persulfidation of L-CYSTEINE DESULFHYDRASE1(DES1)and the production of H2S in guard cells.However,it remains largely unclear how H2S and protein persulfidation participate in the relay of ABA signals.In this study,we discovered that ABSCISIC ACID INSENSITIVE 4(ABI4)acts downstream of DES1 in the control of ABA responses in Arabidopsis.ABI4 undergoes persulfidation at Cys250 that is triggered in a time-dependent manner by ABA,and loss of DES1 function impairs this process.Cys250 and its persulfidation are essential for ABI4 function in the regulation of plant responses to ABA and the H2S donor NaHS during germination,seedling establishment,and stomatal closure,which are abolished in the ABI4Cys250Ala mutated variant.Introduction of the ABI4Cys250Ala variant into the abi4 des1 mutant did not rescue its hyposensitivity to ABA.Cys250 is critical for the binding of ABI4 to its cognate motif in the promoter of Mitogen-Activated Protein Kinase Kinase Kinase 18(MAPKKK18),which propagates the MAPK signaling cascade induced by ABA.Furthermore,the DES1-mediated persulfidation of ABI4 enhances the transactivation activity of ABI4 toward MAPKKK18,and ABI4 can bind the DES1 promoter,forming a regulatory loop.Taken together,these findings advance our understanding of a post-translational regulatory mechanism and suggest that ABI4 functions as an integrator of ABA and MAPK signals through a process in which DES1-produced H2S persulfidates ABI4 at Cys250. | Mingjian Zhou Jing Zhang Jie Shen Heng Zhou Didi Zhao Cecilia Gotor Luis CRomero Ling Fu Zongmin Li Jing Yang Wenbiao Shen Xingxing Yuan Yanjie Xie | 2021 | Molecular Plant2021,14,6: | 5 |
| 10 | Electrical Properties and Micro-structures of Water-tree Aged XLPE Cables After Siloxane Fluid Injection显示文摘 | ZHOU Kai XIONG Qing ZHAO Wei WEN Weijie TAO Wenbiao | 2015 | 高电压技术2015,41,8: | 4 |
| 11 | Carbon isotope fractionation during shale gas transport: Mechanism, characterization and significance显示文摘The gas in-place(GIP)content and the ratio of adsorbed/free gas are two key parameters for the assessment of shale gas resources and have thus received extensive attention.A variety of methods have been proposed to solve these issues,however none have gained widespread acceptance.Carbon isotope fractionation during the methane transport process provides abundant information,serving as an effective method for differentiating the gas transport processes of adsorbed gas and free gas and ultimately evaluating the two key parameters.In this study,four stages of methane carbon isotope fractionation were documented during a laboratory experiment that simulated gas transport through shale.The four stages reflect different transport processes:the free gas seepage stage(Ⅰ),transition stage(Ⅱ),adsorbed gas desorption stage(Ⅲ)and concentration diffusion stage(Ⅳ).Combined with the results of decoupling experiments,the isotope fractionation characteristics donated by the single effect(seepage,adsorption-desorption and diffusion)were clearly revealed.We further propose a technique integrating the Amoco curve fit(ACF)method and carbon isotope fractionation(CIF)to determine the dynamic change in adsorbed and free gas ratios during gas production.We find that the gases produced in stage Ⅰ are primarily composed of free gas and that carbon isotope ratios of methane(δ13C1)are stable and equal to the ratios of source gas(13C 10).In stage Ⅱ,the contribution of free gas decreases,while the proportion of adsorbed gas increases,and the δ13C1 gradually becomes lighter.With the depletion of free gas,the adsorbed gas contribution in stage Ⅲ reaches 100%,and the δ13C1 becomes heavier.Finally,in stage Ⅳ,the desorbed gas remaining in the pore spaces diffuses out under the concentration difference,and the δ13C1 becomes lighter again and finally stabilizes.In addition,a kinetic model for the quantitative description of isotope fractionation during desorption and diffusion was established. | Wenbiao LI Shuangfang LU Junqian LI Pengfei ZHANG Siyuan WANG Wenjun FENG Yongbo WEI | 2020 | Science China Earth Sciences2020,63,5: | 4 |
| 12 | The safety parameters monitoring system for the coal mine based on CAN bus communication and intelligent data acquisition显示文摘In this paper,a monitoring and controlling system for the safety in production and environmental parameters of a small and medium-sized coal mine has been developed after analyzing the current domestic coal production and security conditions. The client computer can convert the analog signal about the safety in production and environmental parameters detected from the monitoring terminal into digital signal,and then,send the signal to the coal mine safety monitoring centre. This information can be analyzed,judged,and diagnosed by the monitoring-management-controlling software for helping the manager and technical workers to control the actual underground production and security situations. The system has many advantages including high reliability,better performance of real-time monitoring,faster data communicating and good practicability,and it can effectively prevent the occurrence of safety incidents in coal mines. | Bin Guangfu Chu Wangwen Balbir S. Dhillon He Wenbiao | 2008 | Engineering Sciences2008,6,4: | 4 |
| 13 | Robust design and analysis of a conformal expansion nozzle with inverse-design idea显示文摘This paper examines robust optimization design and analysis of a conformal expansion nozzle of flying wing Unmanned Aerial Vehicle(UAV) with the inverse-design idea.In view of flow features and stealth constraints, the inverse-design idea is described and the uncertainty-based robust design model is presented.A robust design system employs this model to combine deterministic optimization and robust optimization and is applied into design of a conformal expansion nozzle.The results indicate that design optimization can conform to the anticipation of the inversedesign idea and significantly improve the aerodynamic performance that meet the requirement of 6σ.The present method is a feasible nozzle design strategy that integrates robust optimization and inverse-design. | Wenbiao GAN Xiaocui ZHANG Tielin MA Qinling ZHANG Wu YUAN | 2018 | Chinese Journal of Aeronautics2018,31,1: | 3 |
| 14 | Mechanism on minimization of excess sludge in oxic-settling- anaerobic (OSA) process显示文摘The oxic-settling-anaerobic(OSA)process is a promising wastewater treatment technique for efficiently reducing sludge production and improving the stability of process operation.In this paper,the possible factors of sludge reduction such as sludge decay,uncoupled metabol-ism,and anaerobic oxidation with low sludge production were discussed in the OSA process.It has been confirmed that sludge decay is the decisive cause in the OSA process,accounting for 66.7%of sludge production reduction.Sludge decay includes hydrolysis and acidogenesis of dead microorganisms and particle organic carbon adsorbed in sludge floc and endogenous metabolism.By batch experi-ments,it has been proven that there is energetic uncoupling in the OSA system since microorganisms were exposed to alternative anaerobic and aerobic environment.It accounts for about 7.5%of sludge production reduction.Soluble chemical oxygen demand(SCOD)released from the anaerobic sludge tank in the OSA process was used as the substrate for cryptic growth.The substrate was used for anoxic denitrifying,anaerobic phosphorus release,sulfate reduction,and methane production.These anaerobic reactions in the sludge anaerobic tank have lower sludge production than in the aerobic oxidation when equivalent SCOD is consumed,which may lead to approximately 23%of sludge reduction in the OSA process.It has been concluded that multiple causes resulted in the minimization of excess sludge in the OSA system.The microbial community structure and diversity of sludge samples from the CAS(conventional activated sludge)and OSA systems were investigated by 16 SrDNA PCR-DG-DGGE(poly-merase chain reaction-double gradient-denaturing gradient gel electrophoresis).DGGE profile and cluster analysis showed more abundant species in the OSA system contrasting to microbial communities in the CAS system. | Jianfang WANG Qingliang ZHAO Wenbiao JIN Jikan LIN | 2008 | Frontiers of Environmental Science & Engineering2008,2,1: | 3 |
| 15 | Constraints of f(R) gravity in Palatini approach with observational Hubble data显示文摘We use the newly released observational H(z) data (OHD), the Cosmic Microwave Background (CMB) shift parameter, and the Baryon Acoustic Oscillation (BAO) measurements data to constrain cosmological parameters of f(R) gravity in Palatini formalism in which the f(R) form is defined as f(R) = R β/Rn. Under the assumption of a spatially flat FRW universe, we get the best fitting results of the free parameters (Ωm0, n). In the calculation, we marginalize the likelihood function over H0 by integrating the probability density P ∝ e-χ2/2 to obtain the best fitting results and the confidence regions in the Ωm0-n plane. The constraints results of (Ωm0, n) = (0.33, 0.41) by OHD only and (Ωm0, n) = (0.23, 0.08) by the combination of OHD+CMB+BAO both indicate that the universe goes through three last phases, i.e., radiation dominated, matter-dominated, and late time accelerated expansion without introduction of dark energy. | ZHAI ZhongXu LIU WenBiao | 2011 | Science China(Physics,Mechanics & Astronomy)2011,54,8: | 3 |
| 16 | Abundance and diversity of ammonia-oxidizing archaea in response to various habitats in Pearl River Delta of China, a subtropical maritime zone显示文摘Ammonia-oxidizing archaea(AOA) are widely considered key to ammonia oxidation in various environments.However,little work has been conducted to simultaneously investigate the abundance and diversity of AOA as well as correlations between archaeal amoA genotypes and environmental parameters of different ecosystems at one district.To understand the abundance,diversity,and distribution of AOA in Pearl River Delta of China in response to various habitats,the archaeal amoA genes in soil,marine,river,lake,hot spring and wastewater treatment plant(WWTP) samples were investigated using real-time fluorescent quantitative PCR and clone libraries.Our analyses indicated that the diversity of AOA in various habitats was different and could be clustered into five major clades,i.e.,estuary sediment,marine water/sediment,soil,hot spring and Cluster 1.Phylogenetic analyses revealed that the structure of AOA communities in similar ecological habitats exhibited strong relation.The canonical correspondence method indicated that the AOA community structure was strongly correlated to temperature,pH,total organic carbon,total nitrogen and dissolved oxygen variables.Assessing AOA amoA gene copy numbers,ranging from 6.84 × 106 to 9.45 × 107 copies/g in dry soil/sediment,and 6.06 × 106 to 2.41 × 107 copies/L in water samples,were higher than ammonia-oxidizing bacteria(AOB) by 1-2 orders of magnitude.However,AOA amoA copy numbers were much lower than AOB in WWTP activated sludge samples.Overall,these studies suggested that AOA may be a major contributor to ammonia oxidation in natural habitats but play a minor role in highly aerated activated sludge.The result also showed the ratio of AOA to AOB amoA gene abundance was positively correlated with temperature and less correlated with other environmental parameters.New data from our study provide increasing evidence for the relative abundance and diversity of ammonia-oxidizing archaea in the global nitrogen cycle. | Zhixin Li Wenbiao Jin Zhaoyun Liang Yangyang Yue Junhong Lv | 2013 | Journal of Environmental Sciences2013,25,6: | 3 |
| 17 | Convolution theorem involving n-dimensional windowed fractional Fourier transform显示文摘Dear editor,The fractional Fourier transform(FRFT) was proposed by Wiener in 1929. As an important and powerful analyzing tool for time-frequency analysis, the FRFT has been applied in a lot of fields [1, 2] such as signal processing, optics, radar, and quantum mechanics. According to a similar definition of the FRFT, several other important fractional transforms [3, 4] were studied such as the fractional wavelet transform, the fractional cosine, the fractional sine, and the fractional Hankel transform. | Wenbiao GAO Bingzhao LI | 2021 | Science China(Information Sciences)2021,64,6: | 2 |
| 18 | General radiation via tunneling in Kerr and Kerr-Newman black holes显示文摘Hawking radiation can be viewed as a process of quantum tunneling near the black hole horizon. When a particle with angular momentum L≠ω a tunnels across the event horizon of Kerr or Kerr-Newman black hole, the angular momentum per unit mass a should be changed. The emission rate of the massless particles under this general case is calculated, and the result is consistent with an underlying unitary theory. | GAO Li LIU WenBiao | 2008 | Science China(Physics,Mechanics & Astronomy)2008,51,9: | 2 |
| 19 | Heme Oxygenase-1 is Associated with Wheat Salinity Acclimation by Modulating Reactive Oxygen Species Homeostasis显示文摘Heme oxygenase-1 (HO-1) has been recently identified as an endogenous signaling system in animals. In this study, HO-1 upregulation and its role in acquired salt tolerance (salinity acclimation) were investigated in wheat plants. We discovered that pretreatment with a low concentration of NaCl (25 mmol/L) not only led to the induction of HO-1 protein and gene expression, as well as enhanced HO activity, but also to a salinity acclimatory response thereafter. The effect is specific for HO-1, since the potent HO-1 inhibitor zinc protoporphyrin IX blocks the above cytoprotective actions, and the cytotoxic responses conferred by 200 mmol/L NaCl are reversed partially when HO-1 inducer hemin is added. Heme oxygenase catalytic product, carbon monoxide (CO) aqueous solution pretreatment, mimicked the salinity acclimatory responses. Meanwhile, the CO-triggered re-establishment of reactive oxygen species (ROS) homeostasis was mainly guaranteed by the induction of total and isozymatic activities, or corresponding transcripts of superoxide dismutase, ascorbate peroxidase, and cytosolic peroxidase (POD), as well as the downregulation of NADPH oxidase expression and cell-wall POD activity. A requirement of hydrogen peroxide homeostasis for HO-1-mediated salinity acclimation was also discovered. Taken together, the above results suggest that the upregulation of HO-1 expression was responsible for the observed salinity acclimation through the regulation of ROS homeostasis. | Yanjie Xie Weiti Cui Xingxing Yuan Wenbiao Shen Qing Yang | 2011 | Journal of Integrative Plant Biology2011,53,8: | 2 |
| 20 | Rice GLUTATHIONE PEROXIDASE1-mediated oxidation of bZIP68 positively regulates ABA-independent osmotic stress signaling显示文摘Osmotic stress caused by drought and high salinity is a significant environmental threat that limits plant growth and agricultural yield. Redox regulation plays an important role in plant stress responses, but the mechanisms by which plants perceive and transduce redox signals are still underexplored. Here, we report a critical function for the thiol peroxidase GPX1 in osmotic stress response in rice, where it serves as a redox sensor and transducer. GPX1 is quickly oxidized upon exposure to osmotic stress and forms an intramolecular disulfide bond, which is required for the activation of bZIP68, a VRE-like basic leucine zipper (bZIP) transcription factor involved in the ABA-independent osmotic stress response pathway. The disulfide exchange between GPX1 and bZIP68 induces homo-tetramerization of bZIP68 and thus positively regulates osmotic stress response by regulating osmotic-responsive gene expression. Furthermore, we discovered that the nuclear translocation of GPX1 is regulated by its acetylation under osmotic stress. Taken together, our findings not only uncover the redox regulation of the GPX1-bZIP68 module during osmotic stress but also highlight the coordination of protein acetylation and redox signaling in plant osmotic stress responses. | Heng Zhou Feng Zhang Fengchao Zhai Ye Su Ying Zhou Zhenglin Ge Priyadarshini Tilak Jürgen Eirich Iris Finkemeier Ling Fu Zongmin Li Jing Yang Wenbiao Shen Xingxing Yuan Yanjie Xie | 2022 | Molecular Plant2022,15,4: | 2 |