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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 3 | Effective gene editing by high-fidelity base editor 2 in mouse zygotes显示文摘通过相应再结合的指向的点 mutagenesis 广泛地在基因研究被使用了并且为在病人修理引起疾病的变化保持可观的诺言。然而,象 mosaicism 那样的问题和低 mutagenesis 效率继续提出挑战到如此的途径的临床的申请。最近,一个基础编辑器() 在 cytidine (C) 脱氨基酶上造的系统和 CRISPR/Cas9 技术在植物,酵母,和人的房间为指向的点 mutagenesis 作为一个其他的方法被开发。然而,基础编辑器高效地在 deamination 窗口中把 C 变换成 thymidine (T) 是否指向了在鼠标胚胎的基础编辑,仍然保持不清楚是可行的。在这份报告,我们产生了基础编辑的一个修改高保真版本 2 (HF2-BE2 ) ,并且调查了它在老鼠胚胎编辑功效的底。我们发现 HF2-BE2 能高效地把 C 变换成 T,与直到在老鼠胚胎的 100% biallelic 变化效率。不同于 BE3, HF2-BE2 能在目标和非目标海滨上把 C 变换成 T,扩展基础编辑器的编辑范围。令人惊讶地,我们发现 HF2-BE2 能也使脱去氨基对 gRNA 有约束力的区域近似的 C。一起拿,我们的工作表明由基础编辑,和下划线在鼠标产生点变化的可行性小心地优化编辑系统以便消除近似地点的 deamination 的底的需要。 | Puping Liang Hongwei Sun Ying Sun Xiya Zhang Xiaowei Xie Jinran Zhang zhen Zhang Yuxi Chen Chenhui Ding Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2017 | Protein & Cell2017，8，8： | 17 |
| 4 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 5 | 基于脉冲激励的地铁运营引起邻近建筑物内振动预测方法显示文摘根据振动系统传递特性,提出一种新的环境振动预测方法——实测脉冲传递函数预测方法。通过脉冲试验测得振源与邻近建筑物内测点的振动加速度,计算得到振动系统的振动响应传递函数;将实测的地铁运营振源的振动加速度代入传递函数公式,实现对建筑物内测点的振动加速度时程、频谱、1/3倍频程和z振级的预测。应用该方法预测隧道中心楼内测点振动加速度结果表明:隧道结构与隧道中心楼内测点振动加速度传递函数峰值频段为30~50Hz,隧道中心楼内振动加速度峰值频段为40~60Hz;土层和隧道中心楼基础结构对60Hz以上频段的振动具有很强的衰减作用,楼房基础结构有效地隔断了70Hz以上的高频振动。该方法可用于预测地铁运营引起的建筑物内振动。 | Wang, Wenbin Liu, Weining Sun, Ning Li, Kefei Liu, Li | 2012 | 中国铁道科学2012，，4： | 12 |
| 6 | NIR-II ?uorescence in vivo confocal microscopy with aggregation-induced emission dots显示文摘Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized. | Wenbin Yu Bing Guo Hequn Zhang Jing Zhou Xiaoming Yu Liang Zhu Dingwei Xue Wen Liu Xianhe Sun Jun Qian | 2019 | Science Bulletin2019，64，6： | 10 |
| 7 | The Assessment of Global Surface Temperature Change from 1850s:The C-LSAT2.0 Ensemble and the CMST-Interim Datasets显示文摘Based on C-LSAT2.0,using high-and low-frequency components reconstruction methods,combined with observation constraint masking,a reconstructed C-LSAT2.0 with 756 ensemble members from the 1850s to 2018 has been developed.These ensemble versions have been merged with the ERSSTv5 ensemble dataset,and an upgraded version of the CMSTInterim dataset with 5°×5°resolution has been developed.The CMST-Interim dataset has significantly improved the coverage rate of global surface temperature data.After reconstruction,the data coverage before 1950 increased from 78%−81%of the original CMST to 81%−89%.The total coverage after 1955 reached about 93%,including more than 98%in the Northern Hemisphere and 81%−89%in the Southern Hemisphere.Through the reconstruction ensemble experiments with different parameters,a good basis is provided for more systematic uncertainty assessment of C-LSAT2.0 and CMSTInterim.In comparison with the original CMST,the global mean surface temperatures are estimated to be cooler in the second half of 19th century and warmer during the 21st century,which shows that the global warming trend is further amplified.The global warming trends are updated from 0.085±0.004℃(10 yr)^(–1)and 0.128±0.006℃(10 yr)^(–1)to 0.089±0.004℃(10 yr)^(–1)and 0.137±0.007℃(10 yr)^(–1),respectively,since the start and the second half of 20th century. | Wenbin SUN Qingxiang LI Boyin HUANG Jiayi CHENG Zhaoyang SONG Haiyan LI Wenjie DONG Panmao ZHAI Phil JONES | 2021 | Advances in Atmospheric Sciences2021，38，5： | 8 |
| 8 | Studies of Primary Angle Qosure Glaucoma in China显示文摘The resent researches on primary angle closure glaucoma (PACG) in China were reviewed. The prevalence of PACG (0.41%) in chinese is between the Caucasian and Eskimos, and chronic type is the dominant type in PACG. However, with the economic development and medical care improvements, the prevalence condition of PACG may change; several researches discovered that the mechanism of PACG, especially the chronic type, is not only because of pupil-blocking but also closely related to the mechanism of crowding of peripheral iris and the anterior location of the ciliary body. We supposed that PACG can be divided into several subtypes based on this machnism. Also, we believe that the use of ultrasound biomicroscopy (UBM) and ophthalmic laser in China has promoted the research of PACG and improved the level of prevention and treatment of PACG. Eye Science 1997; 13 : 120- 124. | Ningli Wang, Wenbin Zhou, Tiancai Ye, Qiang Yu, Mingying Lai, Meihua LeZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China | 1997 | 眼科学报1997，13，3： | 7 |
| 9 | Earth's Temporal Principal Moments of Inertia and Variable Rotation显示文摘Based on the gravity field models EGM96 and EIGEN-GL04C, the Earth's time-dependent principal moments of inertia A, B, C are obtained, and the variable rotation of the Earth is determined. Numerical results show that A, B, and C have increasing tendencies; the tilt of the rotation axis increases 2.1×10-8 mas/yr; the third component of the rotational angular velocity, ω 3, has a decrease of 1.0×10-22 rad/s2, which is around 23% of the present observed value. Studies show in detail that both θ and ω 3 experience complex fluctua-tions at various time scales due to the variations of A, B and C. | SHEN Wenbin CHEN Wei SUN Rong | 2008 | Geo-Spatial Information Science2008，11，2： | 6 |
| 10 | Quantitative Real Time Measurement of Iris Configuration in Human Eyes显示文摘Purpose: To evaluate intraobserver and interobserver reproducibility of real time measurement of iris morphology in living human eyes.Methods: Base on the platform of software (Autocad, version 12), we developed an ultrasound biomicroscopy (UBM) image asisstant measuring system. By using the system, we can perform the iris configuration quantitative measurement in living eyes. The measuring parameters including: iris rest length,radius of iris curvature,and the thickness in different parts of iris. Ten anterior segment images of one normal individual were obtained by a single operator to evaluate the intraobserver reproducibility of image capture, and ten times measurement of one image were performed by a single operator to assess the reproducibilityof image measurement. The measurement of three independent observers were compared to investigate interobserver reproducibility in quantitative measurement. Intraobserver and interobserver reproducibility of measurement were assessed by calculating the | Ningli Wang, Mingying Lai, Wenbin ZhouZhongshan Ophthalmic Center , Sun Yat-sen University of Medical Sciences , Guangzhou 510060 , China | 1997 | 眼科学报1997，13，1： | 6 |
| 11 | Environmentally Tough and Stretchable MXene Organohydrogel with Exceptionally Enhanced Electromagnetic Interference Shielding Performances显示文摘Conductive hydrogels have potential applications in shielding electromagnetic(EM)radiation interference in deformable and wearable electronic devices,but usually suffer from poor environmental stability and stretching-induced shielding performance degradation.Although organohydrogels can improve the environmental stability of materials,their development is at the expense of reducing electrical conductivity and thus weakening EM interference shielding ability.Here,a MXene organohydrogel is prepared which is composed of MXene network for electron conduction,binary solvent channels for ion conduction,and abundant solvent-polymer-MXene interfaces for EM wave scattering.This organohydrogel possesses excellent anti-drying ability,low-temperature tolerance,stretchability,shape adaptability,adhesion and rapid self-healing ability.Two effective strategies have been proposed to solve the problems of current organohydrogel shielding materials.By reasonably controlling the MXene content and the glycerol-water ratio in the gel,MXene organohydrogel can exhibit exceptionally enhanced EM interference shielding performances compared to MXene hydrogel due to the increased physical cross-linking density of the gel.Moreover,MXene organohydrogel shows attractive stretching-enhanced interference effectiveness,caused by the connection and parallel arrangement of MXene nanosheets.This well-designed MXene organohydrogel has potential applications in shielding EM interference in deformable and wearable electronic devices. | Yuanhang Yu Peng Yi Wenbin Xu Xin Sun Gao Deng Xiaofang Liu Jianglan Shui Ronghai Yu | 2022 | Nano-Micro Letters2022，14，5： | 6 |
| 12 | Increased adversely-affected population from water shortage below normal conditions in China with anthropogenic warming显示文摘Whilst climate warming is expected to speed up the water cycle, it is not clear how the renewable freshwater availability and societal impact would change globally and regionally. Recent call for reporting naturally occurring drought with anthropogenic water shortage and the need for new scientific knowledge around the warming targets (e.g., 1.5 and 2°C) prompts us to tackle this challenge [1–4]. Recently, we published a research paper in GeophysicalResearch Letters. | Wenbin Liu Fubao Sun | 2019 | Science Bulletin2019，64，9： | 5 |
| 13 | Effective and precise adenine base editing n mouse zygotes显示文摘 | Puping Liang Hongwei Sun Xiya Zhang Xiaowei Xie Jinran Zhang Yaofu Bai Xueling Ouyang Shengyao Zhi Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2018 | Protein & Cell2018，9，9： | 4 |
| 14 | Late Mesozoic Thermotectonic Evolution of the Jueluotage Range,Eastern Xinjiang,Northwest China:Evidence from Apatite Fission Track Data显示文摘摘要:尽管许多作者强调了变丑的新生代历史,发掘并且在与印度亚洲碰撞有关的 Tianshan 区域冷却,几乎不对压缩的中生代历史被知道并且在 Tianshan 以内高举。以便关于中生代发掘历史和在东方 Tianshan 冷却的进程获得信息,磷灰石上的分裂磁道方法被使用。采样在 Jueluotage 范围被做。三件样品(Z001-Z003 ) 在 Yandong pluton 的地上凿穿 ZK6301 从花岗石被拿;从比是的 pluton 年龄年轻得多的 97.0 ~ 87.6 妈的年龄范围在 334 点由 U-Pb 锆石标明日期 ????????? Y ?? 霈吗?? | ZHU Wenbin WAN Jinglin SHU Liangshu ZHANG Zhiyong SU Jinbao SUN Yah GUO Jichun ZHANG Xueyun | 2008 | Acta Geologica Sinica(English Edition)2008，82，2： | 4 |
| 15 | Cuprous Oxide/Nitrogen-doped Graphene Nanocomposites as Electrochemical Sensors for Ofloxacin Determination显示文摘 | WU Fanghui XU Fan CHEN Le JIANG Binbin SUN Wenbin WEI Xianwen | 2016 | Chemical Research in Chinese Universities2016，32，3： | 3 |
| 16 | Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt显示文摘A cDNAderived 200 bp fragment encoding a rabbit defensin NP1 mature peptide was ligated into the GUSless pBI121 to yield pBIC35NP1. Tobacco leaf discs were genetically transformed with an Agrobacterium tumefaciens strain harbouring pBIC35NP1. Northern blot analysis showed that the defensin NP1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP1 can confer partial resistance to the bacterial wilt pathogen, Ralstonia solanacearum. | Rongzhao Fu Yufa Peng Guangcheng Cao Jiangsheng Ma Caixia Chen Liming Zhang Wenbin Li Yongru Sun | 1998 | Chinese Science Bulletin1998，43，18： | 3 |
| 17 | Metal cation removal by P(VC-r-AA) copolymer ultrafiltration membranes显示文摘poly 包含的一系列 amphiphilic 共聚物(乙烯基 chloride-r-acrylic 酸)(P (VC-r-AA )) 被综合并且过去常经由 nonsolvent 准备膜导致的阶段分离方法。准备的膜被扫描电子显微镜学, X 光检查光电子光谱学,和水接触角度和希腊语的第六个字母潜力大小描绘。共聚物 P (VC-r-AA ) 链溶解在凝结洗澡,显示 AA 分割完全在膜以内被保留。在表面层充实 AA 片断的度为共聚物膜是 2。另外, AA 片断的介绍使膜阴电、吸水以便膜对答案 pH 敏感。犯规抵抗, Cu (II ) 的吸附, Cr (III ) 和 Ce (IV ) 离子和膜的解吸附作用性质也是坚定的。共聚物膜与 92% 的犯规可逆性展出了好 antifouling 性能。膜也为 Cu (II ) 有好吸附能力, Cr (III ) 和 Ce (IV ) 离子。为 Cu (II ) 吸附的最佳的 pH 是 6,共聚物膜为低集中 Cu (II ) 移动有潜在的应用。 | Nachuan Wang Jun Wang Peng Zhang Wenbin Wang Chuangchao Sun Ling Xiao Chen Chen Bin Zhao Qingran Kong Baoku Zhu | 2018 | Frontiers of Chemical Science and Engineering2018，12，2： | 3 |
| 18 | An Analytic and Optimal Inverse Kinematic Solution for a 7-DOF Space Manipulator显示文摘An analytic inverse kinematic solution is presented for a 7-DOF(degree of freedom)redundant space manipulator.The proposed method can obtain all the feasible solutions in the global joint space,which are denoted by a joint angle parameter.Meanwhile,both the singularity problem and the joint limits are considered in detail.Besides,an optimization approach is provided to get one near optimal inverse kinematic solution from all the feasible solutions.The proposed method can reduce effectively the computational complexity,so that it can be applied online.Finally,the method’s validity is shown by kinematic simulations. | WANG Yingshi | 2014 | 机器人2014，36，5： | 3 |
| 19 | Using nerve segment insert grafting to reconstruct neural pathways of brain-derived paralysis显示文摘Since 1992,task groups have used free nerve auto grafts to bridge partially transected nerves from the dominant area of the normal cerebral cortex to nerves that innervate spastic muscles from a diseased cerebral cortex,using transplanting sutures to alleviate the muscle spasm of cerebral palsy caused by different diseases. This has facilitated rebuilding of some of its neurological function. In this study,80 such patients were followed up,including 20 patients with traumatic brain injury,32 patients with stroke,and 28 pediatric patients with cerebral palsy. After postoperative follow-up of 3 to 21 years,the efficacy rate of this operation was 100% and the excellent and good spasm relief rate was 82.5%. | Wenbin Ding Shaocheng Zhang Zhuo Wang Lin Chen Chuansen Zhang Ping Huang Shunfa Liu Laiqing Sun Yuhai Ma Jun Yang Fei Huang Chengjing Xue Yanxue Zhong Lei Yin Yongtai Pan Dajiang Wu | 2017 | Translational Neuroscience and Clinics2017，3，4： | 2 |
| 20 | Stable perovskite solar cells with efficiency of 22.6%via quinoxaline-based polymeric hole transport material显示文摘Poor stability of spiro-OMe TAD hole transport materials(HTMs)with dopant is a major obstacle for the commercialization of perovskite solar cells(pero-SCs).Herein,we demonstrate a series of quinoxaline-based D-A copolymers PBQ5,PBQ6 and PBQ10 as the dopant-free polymer HTMs for high performance pero-SCs.The D-A copolymers are composed of fluorothienyl benzodithiophene(BDTT)as D-unit,difluoroquinoxaline(DFQ)with different side chains as A-unit,and thiophene asπ-bridge,where the side chains on the DFQ unit are bi-alkyl for PBQ5,bi-alkyl-fluorothienyl for PBQ6,and alkoxyl for PBQ10.All the three copolymers are adopted as the dopant-free HTM in the pero-SCs.The planar n-i-p structured pero-SCs based on(FAPb I_(3))_(0.98)(MAPb Br_(3))_(0.02)with PBQ6 HTM demonstrated the high power conversion efficiency(PCE)of 22.6%with Vocof1.13 V and FF of 80.8%,which is benefitted from the suitable energy level and high hole mobility of PBQ6.The PCE of 22.6%is the highest efficiency reported in the n-i-p structured pero-SCs based on dopant-free D-A copolymer HTM.In addition,the peroSCs show significantly enhanced ambient,thermal and light-soaking stability compared with the devices with traditional spiroOMe TAD HTM. | Chenxing Lu Can Zhu Lei Meng Chenkai Sun Wenbin Lai Shucheng Qin Jinyuan Zhang Wenchao Huang Jiaqi Du Yiyang Wang Yongfang Li | 2021 | Science China Chemistry2021，64，11： | 2 |
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