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| 1 | In vivo transfection of enhanced green fluorescent protein in rat retinal ganglion cells mediated by ultrasound-induced microbubbles显示文摘BACKGROUND:Studies have demonstrated that ultrasound-mediated microbubble destruction significantly improves transfection efficiency of enhanced green fluorescent protein(EGFP) in in vitro cultured retinal ganglial cells(RGCs). OBJECTIVE:To investigate the feasibility of ultrasound-mediated microbubble destruction for EGFP transfection in rat RGCs,and to compare efficiency and cell damage with traditional transfection methods. DESIGN,TIME AND SETTING:In vivo,gene engineering experiment.The study was performed at the Central Laboratory,Institute of Ultrasonic Imaging,Chongqing Medical University from March to July 2008. MATERIALS:Eukaryotic expression vector plasmid EGFP and microbubbles were prepared by the Institute of Ultrasonic Imaging,Chongqing Medical University.The microbubbles were produced at a concentration of 8.7×10^(11)/L,with a 2-4μm diameter,and 10-hour half-life in vitro. METHODS:A total of 50 Sprague Dawley rats were randomly assigned to four groups.Normal controls(n=5) were infused with 5μL normal saline to the vitreous cavity;the naked plasmid group (n=15) was infused with 5μL EGFP plasmid to the vitreous cavity;in the plasmid with ultrasound group(n=15),the eyes were irradiated with low-energy ultrasound wave(0.5 W/cm^2) for a total of 60 seconds(irradiated for 5 seconds,at 10-second intervals) immediately following infusion of EGFP plasmids to the vitreous cavities.In the microbubble-ultrasound group(n=15),the eyes were irradiated with the same power of ultrasonic wave immediately following infusion of microbubbles containing EGFP plasmids to the vitreous cavities. MAIN OUTCOME MEASURES:After 7 days,retinal preparations and EGFP expression in RGCs were observed by fluorescence microscopy.RGC quantification in the retinal ganglion cell layer was performed.In addition,EGFP mRNA expression was semi-quantitatively determined by RT-PCR. RESULTS:The transfection efficiency of EGFP to RGCs by microbubbles with ultrasound was significantly greater than the other groups,and no obvious damage was detected in the RGCs. CONCLUSION:Under irradiation of low-frequency ultrasound waves,ultrasound-mediated microbubble destruction was effective and resulted in safe transfection of the EGFP gene to the RGCs. | Hong Su Su Liu Zhigang Wang Wenyue Xie Bing Jiang Haibo Xiong | 2009 | Neural Regeneration Research2009，4，6： | 3 |
| 2 | Hollow click-based porous organic polymers for heterogenization of [Ru(bpy)3]2+ through electrostatic interactions显示文摘为用在空基于按的多孔的器官的聚合物(H-CPPs ) 的非共有原子价相互作用的转变金属催化剂的 heterogenization 的一条灵巧的途径被介绍。催化地活跃的 cationic 种,[Ru (bpy )3]2+(bpy = 2,2-bipyridyl ) ,经由静电的相互作用在 H-CPPs 被使不能调动。内在的性质[Ru (bpy )3]2+ 很好被保留。当在可见光的照耀下面为 4-methoxyphenylboronic 酸的氧化 hydroxylation 使用了到 4-methoxyphenol 时,产生 Rucontaining 空聚合物展出了优秀催化活动,提高的稳定性,和好再循环能力。吸引人的催化表演主要从有效集体转移和 cationic Ru 建筑群的化学性质的维护导致了 H-CPPs。 | Liuyi Li Caiyan Cui Wenyue Su Yangxin Wang Ruihu Wang | 2016 | Nano Research2016，9，3： | 2 |
| 3 | Self-assembly synthesis of LAPO4 hierarchical hollow spheres with enhanced photocatalytic CO2-reduction performance显示文摘像顽童的 LaPO 4 空范围被一条灵巧的答案线路成功地作为一个指导结构的代理人用柠檬性的酸(CA ) 综合。尺寸三维(3D ) 空范围被改变 CA 的集中调节。3D LaPO 4 的形成机制空范围被学习时间依赖者形态学进化过程揭示。重要地,与 monodispersed 相比一个维(1D ) LaPO 4 nanorods,从 nanorods 自我装配的空范围显示出的 3D LaPO 4 在为公司 2 减小的 photocatalytic 活动的 6.8 褶层改进,它被归因于他们的层次空结构,更高轻收获的能力,和更快的电子转移的 synergistic 效果。我们的调查结果为提高 photocatalytic 表演为层次空 micro/nanoarchitectures 而且一条有效线路的合成提供一个简单、灵巧的方法不仅。 | Bao Pan Yangen Zhou Wenyue Su Xuxu Wang | 2017 | Nano Research2017，10，2： | 2 |
| 4 | Analysis of Trace-Level Volatile Compounds in Fresh Turf Crop (Lolium perenne L.) by Gas Chromatography Quadrupole Time-of-Flight Mass Spectrometry显示文摘在不稳定的混合物的传统的研究,一些踪迹级的混合物不能被煤气的层析团 spectrometry 识别。目标和 targeted 以后方法与混合四极 time-of-flight 团 spectrometry 在联合基于煤气的层析在新鲜草地庄稼(Lolium perenne L.) 在踪迹级的不稳定的混合物的调查被使用。根据出版的文学,目标分析被使用保留索引,典型离子的精确群众和秒阶段团系列(MS2 系列) 执行。并且一系列实验证明低电子冲击能对许多的改进有益低丰富分子的离子山峰。提高许多分子的离子山峰是的低丰富对质的分析有益。完全, 60 不稳定的混合物被识别,哪个是 benzeneacetaldehyde (14.8%) 的大多数混合物, 2,5-dimethyl-pyrazine (9.6%) ,和 hexanal (9.3%) 。鉴定被质量应允光谱搜索,保留索引和典型离子的精确群众。 | Saisai Hong Wenyu Kang Yue Su Yinlong Guo | 2013 | Chinese Journal of Chemistry2013，31，10： | 2 |
| 5 | Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress,protecting mitochondrial function,and regulating metabolic pathways显示文摘The development of acute liver injury can result in liver cirrhosis,liver failure,and even liver cancer,yet there is currently no effective therapy for it.The purpose of this study was to investigate the protective effect and therapeutic mechanism of Lycium barbarum polysaccharides(LBPs)on acute liver injury induced by carbon tetrachloride(CCl_(4)).To create a model of acute liver injury,experimental canines received an intraperitoneal injection of 1 mL/kg of CCl_(4)solution.The experimental canines in the therapy group were then fed LBPs(20 mg/kg).CCl_(4)-induced liver structural damage,excessive fibrosis,and reduced mitochondrial density were all improved by LBPs,according to microstructure data.By suppressing Kelch-like epichlorohydrin(ECH)-associated protein 1(Keap1),promoting the production of sequestosome 1(SQSTM1)/p62,nuclear factor erythroid 2-related factor 2(Nrf2),and phaseⅡdetoxification genes and proteins downstream of Nrf2,and restoring the activity of anti-oxidant enzymes like catalase(CAT),LBPs can restore and increase the antioxidant capacity of liver.To lessen mitochondrial damage,LBPs can also enhance mitochondrial respiration,raise tissue adenosine triphosphate(ATP)levels,and reactivate the respiratory chain complexes I–V.According to serum metabolomics,the therapeutic impact of LBPs on acute liver damage is accomplished mostly by controlling the pathways to lipid metabolism.9-Hydroxyoctadecadienoic acid(9-HODE),lysophosphatidylcholine(Lyso PC/LPC),and phosphatidylethanolamine(PE)may be potential indicators of acute liver injury.This study confirmed that LBPs,an effective hepatoprotective drug,may cure acute liver injury by lowering oxidative stress,repairing mitochondrial damage,and regulating metabolic pathways. | JIANJIA HUANG YUMAN BAI WENTING XIE RONGMEI WANG WENYUE QIU SHUILIAN ZHOU ZHAOXIN TANG JIANZHAO LIAO RONGSHENG SU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，2： | 2 |
| 6 | Arginine methylation of ribose-5-phosphate isomerase A senses glucose to promote human colorectal cancer cell survival显示文摘Cancer cells remodel their metabolic network to adapt to variable nutrient availability. Pentose phosphate pathway(PPP) plays protective and biosynthetic roles by oxidizing glucose to generate reducing power and ribose. How cancer cells modulate PPP activity in response to glucose supply remains unclear. Here we show that ribose-5-phosphate isomerase A(RPIA), an enzyme in PPP, directly interacts with co-activator associated arginine methyltransferase 1(CARM1) and is methylated at arginine 42(R42). R42 methylation up-regulates the catalytic activity of RPIA. Furthermore, glucose deprivation strengthens the binding of CARM1 with RPIA to induce R42 hypermethylation. Insufficient glucose supply links to RPIA hypermethylation at R42, which increases oxidative PPP flux. RPIA methylation supports ROS clearance by enhancing NADPH production and fuels nucleic acid synthesis by increasing ribose supply. Importantly, RPIA methylation at R42 significantly potentiates colorectal cancer cell survival under glucose starvation. Collectively, RPIA methylation connects glucose availability to nucleotide synthesis and redox homeostasis. | Jizheng Guo Qixiang Zhang Ying Su Xiaochen Lu Yiping Wang Miao Yin Weiguo Hu Wenyu Wen Qun-Ying Lei | 2020 | Science China(Life Sciences)2020，63，9： | 2 |
| 7 | Probing of photocatalytic surface sites on SO 4 2? /TiO 2 solid acids by in situ FT-IR spectroscopy and pyridine adsorption显示文摘 | Xinchen Wang Jimmy C. Yu Ping Liu Xuxu Wang Wenyue Su Xianzhi Fu | 2005 | Journal of Photochemistry & Photobiology, A: Chemistry2005，，3： | 1 |
| 8 | Plasma pretreatment and TiO2 coating of PMMA for the improvement of antibacterial properties显示文摘 | Su Wenyue Wang Shichao Wang Xuxu | | 0，，： | 1 |
| 9 | 显示文摘 | Fu Xianzhi Wei Kemei | 2001 | Huanjing Kexue2001，22，2： | 1 |
| 10 | Multivalency iodine doped TiO2:preparation,characterization,theoretical studies,and visible-light photocatalysis显示文摘 | Su Wenyue Zhang Yongfan Li Zhaohui | 2008 | Langmuir2008，24，7： | 1 |
| 11 | Evaluation of chitosan/PVA blended hydrogel membranes显示文摘 | Yang Jenming Su Wenyu Leu T L | 2004 | Journal of Membrane Science2004，236，12： | 1 |
| 12 | Visible light photocatalysis on praseodymi- um (III)-nitrate-modified TiO2 prepared by an ultrasonnd method 显示文摘 | Su Wenyue Chen Jianxiong Wu Ling Wang Xinchen Wang Xuxu Fu Xianzhi | 2008 | Applied Catalysis B: Environmental2008，77，： | 1 |
| 13 | Recursive High Resolution Reconstruction of Blurred Multiframe Images显示文摘 | Kim S P Su Wenyu | 1993 | IEEE Transactionson Image Processing1993，2，4： | 1 |
| 14 | OBV(obscure vein),a C_(2)H_(2)zinc finger transcription factor,positively regulates chloroplast development and bundle sheath extension formation in tomato(Solanum lycopersicum)leaf veins显示文摘Leaf veins play an important role in plant growth and development,and the bundle sheath(BS)is believed to greatly improve the photosynthetic efficiency of C_(4) plants.The OBV mutation in tomato(Solanum lycopersicum)results in dark veins and has been used widely in processing tomato varieties.However,physiological performance has difficulty explaining fitness in production.In this study,we confirmed that this mutation was caused by both the increased chlorophyll content and the absence of bundle sheath extension(BSE)in the veins.Using genome-wide association analysis and map-based cloning,we revealed that OBV encoded a C_(2)H_(2) L domain class transcription factor.It was localized in the nucleus and presented cell type-specific gene expression in the leaf veins.Furthermore,we verified the gene function by generating CRISPR/Cas9 knockout and overexpression mutants of the tomato gene.RNA sequencing analysis revealed that OBV was involved in regulating chloroplast development and photosynthesis,which greatly supported the change in chlorophyll content by mutation.Taken together,these findings demonstrated that OBV affected the growth and development of tomato by regulating chloroplast development in leaf veins.This study also provides a solid foundation to further decipher the mechanism of BSEs and to understand the evolution of photosynthesis in land plants. | Jinghua Lu Chunyang Pan Xin Li Zejun Huang Jinshuai Shu Xiaoxuan Wang Xiaoxiao Lu Feng Pan Junling Hu Hui Zhang Wenyue Su Min Zhang Yongchen Du Lei Liu Yanmei Guo Junming Li | 2021 | Horticulture Research2021，8，1： | 1 |
| 15 | Recursive high - resolution reconstruction of blurred multiframe images显示文摘 | Kim S P Su Wenyu | 1993 | IEEE Transactions on Image Processing1993，2，4： | 1 |
| 16 | Elevated levels of Th17 cells and Th17-related cytokines are associated with disease activity in patients with inflammatory bowel disease显示文摘 | Wenyu Jiang Jiewen Su Xiaofei Zhang Xiuqin Cheng Jun Zhou Ruihua Shi Hongjie Zhang | 2014 | Inflammation Research2014，，11： | 1 |
| 17 | Application of gas chromatography-quadrupole- time-of-flight-nmss spectrometry for post-target analysis of volatile compounds in Fructus Amomi 显示文摘 | KANG Wenyu ZHANG Fang SU Yue | 2013 | Euro J Mass Spec2013，19，2： | 1 |
| 18 | Multi valency iodine doped Ti02: preparation, characteriza tion, theoretical studies, and visible light photocatalysi 显示文摘 | Su Wenyue Zhang Yongfan Li Zhaohui | 2008 | Langmuir2008，24，7： | 1 |
| 19 | RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunits显示文摘RBM46 is a germ cell-specific RNA-binding protein required for gametogenesis,but the targets and molecular functions of RBM46 remain unknown.Here,we demonstrate that RBM46 binds at specific motifs in the 3'UTRs of mRNAs encoding multiple meiotic cohesin subunits and show that RBM46 is required for normal synaptonemal complex formation during meiosis initiation.Using a recently reported,high-resolution technique known as LACE-seq and working with low-input cells,we profiled the targets of RBM46 at single-nucleotide resolution in leptotene and zygotene stage gametes.We found that RBM46 preferentially binds target mRNAs containing GCCUAU/GUUCGA motifs in their 3'UTRs regions.In Rbm46 knockout mice,the RBM46-target cohesin subunits displayed unaltered mRNA levels but had reduced translation,resulting in the failed assembly of axial elements,synapsis disruption,and meiotic arrest.Our study thus provides mechanistic insights into the molecular functions of RBM46 in gametogenesis and illustrates the power of LACE-seq for investigations of RNA-binding protein functions when working with low-abundance input materials. | Yue Lv Gang Lu Yuling Cai Ruibao Su Liang Liang Xin Wang Wenyu Mu Xiuqing He Tao Huang Jinlong Ma Yueran Zhao Zi-Jiang Chen Yuanchao Xue Hongbin Liu Wai-Yee Chan | 2023 | Protein & Cell2023，14，1： | 0 |
| 20 | Identification of a novel efficient transcriptional activation domain from Chinese fir(Cunninghamia lanceolata)显示文摘Direct manipulation of gene expression in vivo is a powerful approach for investigating biological systems and bioengineering in plants(Zhang et al.,2015;Zhao et al.,2015;Lowder et al.,2018).In the past decade,considerable progress has been made in developing techniques for gene targeting. | Tengfei Zhu Wenyu Tang Delan Chen Jian Li Jun Su | 2021 | Journal of Genetics and Genomics2021，48，3： | 0 |
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