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| 1 | Studies of poly-L-lysine-starch nanoparticle preparation and its application as gene carrier显示文摘Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in wa- ter-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we devel- oped the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection effi- ciency, low cell toxicity and biodegradability. | XIAO Suyao LIU Xuanming TONG Chunyi LIU Jun TANG Dongying ZHAO Lijian | 2005 | Science China Chemistry2005，48，2： | 7 |
| 2 | Dialdehyde starch nanoparticles: Preparation and application in drug carrier显示文摘Dialdehyde starch nanoparticles (DASNP) were prepared by the redox reaction of NaIO4 and starch in water-in-oil microemulsion. IR spectrum showed that DASNP had aldehyde groups, and quantitative alkali consumption showed that its dialdehyde content was about (50±5)%. The average diameter of DASNP determined by SEM was about 100 nm. TGA-DTA showed that its thermal stability was better than starch nanoparticle (StNP) and dialdehyde starch (DAS). Its low biological toxicity was detected by cell experiment. Also the best mass ratio of doxorubicin (DOX) to combined DASNP detected by UV-VIS was 15 : 1, and the product was effective for controlled release of DOX. The cell experiment showed that the drug-carrier particle (DOX-DASNP) can release DOX for a long time and strengthened the effect of the anticancer drug. This work demonstrates that the DASNP, which has good thermal stability, small particle size, low biological toxicity, and slowly anticancer drug-releasing to strengthen drug effect, is a potentially useful carrier for anticancer drug. | YU DanMi XIAO SuYao TONG ChunYi CHEN Lin LIU XuanMing | 2007 | Chinese Science Bulletin2007，52，21： | 5 |
| 3 | Dialdehyde starch nanoparticles as antitumor drug delivery system:An in vitro,in vivo,and immunohistological evaluation显示文摘Sustaining the release of therapeutic nanoparticles in a cell-, tissue-, or disease-specific manner is a potentially powerful technology. A new drug carrier-dialdehyde starch nanoparticle (DASNP) that can sustain the loading and release of 5-fluorouracil (5-Fu) antitumor drug is reported in this study. IR spectrophotometer and 1H NMR confirmed the formation of aldehyde groups, and scan electron microscope determinations showed that the dialdehyde starch nanoparticles obtained had an average diameter of 90 nm. 5-Fu, the model drug, was conjugated into nanoparticles by aldehyde groups. These 5-Fu-binding nanoparticles significantly enhanced breast cancer cell (MCF-7) inhibition in vitro compared with free 5-Fu. After subcutaneous 0 injection in the breast tumor-loaded rats, 5-Fu-DASNP exhibited remarkable tumor-inhibitory efficacy determined by measuring tumor weight in vivo. The tumor inhibition of 5-Fu-DASNP was 61%±6%, whereas that of free 5-Fu was only 42%±4%. Bcl-2/Bax immunohistochem-istry studies indicated that 5-Fu-DASNP remarkably induced tumor tissue necrosis. These results demonstrated that the DASNP prepared in this work is a potentially effective drug carrier. | XIAO SuYao LIU XuanMing TONG ChunYi ZHAO LiChao LIU XiaoJuan ZHOU AiMei CAO Yong | 2012 | Chinese Science Bulletin2012，57，24： | 3 |
| 4 | Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves ( Eucalyptus grandis×Eucalyptus urophylla GL9 )显示文摘 | Yunjiao Chen Junjiang Wang Yangwen Ou Hongzhang Chen Suyao Xiao Guo Liu Yong Cao Qingrong Huang | 2013 | Journal of Functional Foods2013，，： | 1 |
| 5 | The anti-proliferative activity and cellular antioxidant activity of oenothein B and its content in different Eucalyptus species and region显示文摘The leaves of Eucalyptus are abundant in natural resources as a novel potential antioxidant and have broad market prospect.Four of characteristic hydrolysable tannins from Eucalyptus leaves have been demonstrated great application potential as natural antioxidants in our previous research.In this study,the bioactive activities of another hydrolysable tannin isolated and identified as oenothein B(OEB)were evaluated.Content of bioactive compound(hydrolysable tannin)from different Eucalyptus species and regions in southern China were investigated.The results showed that OEB not only exerted anti-proliferative activity against HepG2 cell,but also showed higher cellular antioxidant activity than some common antioxidants.Generally,content of characteristic hydrolysable tannins decreased in the order of OEB>tellimagrandin I(T1)>pedunculagin>gemin D>pentagalloyl glucose(PGG).E.grandis×E.urophylla GL9 and E.urophylla×E.grandis could be recommended as new natural sources to exploit hydrolysable tannins because of high content,widely plantation and strong adaptability.This study supplied first insight on introduction of high cellular antioxidant activity of OEB and selecting the species and region to exploit natural antioxidants.It will promote the increasing of economic value of Eucalyptus. | Yunjiao Chen Qiao Qin Hongzhang Chen Qingrong Huang Mingyue Song Suyao Xiao Longbipei Cao Yong Cao | 2023 | Journal of Future Foods2023，3，4： | 0 |
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