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| 1 | Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells显示文摘 | Shuya Zhou Chenhui Ding Xiaoyang Zhao Eryao Wang Xiangpeng Dai Lei Liu Wei Li Zichuan Liu Haifeng Wan Chunjing Feng Tang Hai Liu Wang Qi Zhou | 2010 | Cell Research2010，20，7： | 10 |
| 2 | Single W18O49 nanowires: A multifunctional nanoplatform for computed tomography imaging and photothermal/ photodynamic/radiation synergistic cancer therapy显示文摘联合治疗是通常基于复杂 nanostructures 的利用,多重部件为 photothermal 作为相片热的精力变换器, photo-sensitizers,或剂量 intensifiers 工作的有希望的癌症治疗策略治疗(PTT ) ,光力学的治疗(太平洋夏季时间) ,或放射治疗(RT ) 。在这研究, ultrathin 钨氧化物 nanowires (W 18 O 49) 用一条 solvothermal 途径被综合并且作为多功能的 theranostic nanoplatform 检验了。在 vitro 并且在 vivo,分析证明这些 nanowires 能导致广泛的热 -- 并且汗衫在 980 nm 在附近下面的癌症房间的调停氧的损坏红外线(NIR ) 激光刺激。他们也被显示作为加强在放射治疗期间局部地并且有选择地提高放热精力免职的代理人的放射剂量工作。独自比作导致 NIR 的 PTT/PDT 和 RT, W 18 O 49-based synergistic tri 形式的治疗根除了异种皮移植肿瘤和没有复发被观察在以内一 9 月列在后面起来。而且,钨元素的强壮的 X 光检查变细能力(Z = 74, 4.438 厘米 2???? ?? ?? 佦 ? 畳 ?? 猯扵￣?? | Jianjian Qiu Qingfeng Xiao Xiangpeng Zheng Libo Zhang Huaiyong Xing Dalong Ni Yanyan Liu Shengjian Zhang Qingguo Ren Yanqing Hua Kuaile Zhao Wenbo Bu | 2015 | Nano Research2015，8，11： | 5 |
| 3 | Establishment of customized mouse stem cell lines by sequential nuclear transfer显示文摘治疗学的克隆,胚胎的干细胞(转换字符) 由此从原子转移(NT ) 被导出胚胎,可以在再生药的新时代起一个主要作用。Inthis 学习我们建立了四十原子转移 -- 从不同施主房间类型或段落的 NTembryos 被导出的转换字符(NT 转换字符) 线。我们发现 NT 转换字符能够形成胚胎植物或动物身体。另外, NT 转换字符表示了 pluripotency 干细胞标记试管内并且能区分进胚胎的纸巾体内。从施主房间能形成完整的术语的早经过 R1 的 NT 胚胎开发了小狗,而那些为为实时出生是必要的 reprogramming 从迟了的经过 R1 ES 施主房间失去了潜力。我们随后建立了近来从 NT 胚囊被开发的 sequentialNT-R1-ESC 线经过 R1 转换字符施主。然而,当在他们的早段落用作原子转移施主时,这些 NT-R1-ESC 排队,没能导致实时小狗。这显示用顺序的 NT 转换字符的治疗学的克隆过程不能救居住在以前的施主代的发展缺乏。 | Chunli Zhao Ruqiang Yao Jie Hao Chenhui Ding Yong Fan Xiangpeng Dai Wei Li Tang Hai Zichuan Liu Yang Yu Yingying Wang Xiaojun Hou Weizhi Ji Qi Zhou Alice Jouneau Fanyi Zeng Liu Wang | 2007 | Cell Research2007，17，1： | 1 |
| 4 | Towards the Semantics and Verification of BPEL4WS显示文摘 | Pu Geguang Zhao Xiangpeng Wang Shuling | 2006 | Electronic Notes in Theoretical Computer Science2006，151，2： | 1 |
| 5 | Towards the semantics and verification of BPEL4WS显示文摘 | Pu Geguang Zhao Xiangpeng Wang Shuling | 2006 | Electronic Notes in Theoretical Computer Science2006，151，2： | 1 |
| 6 | Design and performance analysis of tangential-axial flow threshing device for oat harvester显示文摘Aiming at the problems of stalks winding and poor threshing performance in the process of mechanical harvesting of oats,a type of threshing and crushing experimental device was designed.The device was composed of a belt conveyor,a tangential-flow threshing drum,an axial-flow threshing drum,a high-speed camera and a testing device.According to a regression orthogonal test method,using the rotation speed of the axial-flow drum,the horizontal center distance,and the vertical height difference of the two threshing drums as experimental factors;using the mass fraction of long stalks and the threshing rate as experimental indexes,a mathematical regression model of factors and indexes was established,and combined parameters were analyzed for the threshing quality and crushing ability.Experimental results showed that this device had the best crushing performance when the rotation speed of the axial-flow drum was 850 r/min,the horizontal center distance was 820 mm,and the vertical height difference was 10 mm,and this device had optimal threshing performance when the rotation speed of the axial-flow drum was 760 r/min,the horizontal center distance was 820 mm,and the vertical height difference was 20 mm.The parameters were optimized by Design-Expert 11 to obtain an optimization result that the rotation speed of the axial-flow drum was 800 r/min,the horizontal center distance was 820 mm,and the vertical height difference was 10 mm.A verification experiment was carried out by using a high-speed camera,and the two groups of parameters were selected for a comparative experiment.Images of material movement between the two threshing drums were captured by the high-speed camera.Experimental results showed that the optimized parameters were beneficial to improving the threshing performance and anti-winding performance of the oat threshing process.This study provides a technical basis for the research and development of oat combine harvesters. | Rongxian Wang Xiangpeng Zhao Jiangtao Ji Xin Jin Bin Li | 2021 | International Journal of Agricultural and Biological Engineering2021，14，6： | 0 |
| 7 | Algorithms for checking channel passing in web service choreography显示文摘 | Hongli YANG Chao CAI Liyang PENG Xiangpeng ZHAO Zongyan QIU Shengchao QIN | 2013 | Frontiers of Computer Science2013，7，5： | 0 |
| 8 | Cloning efficiency following ES cell nuclear transfer is influenced by the methylation state of the donor nucleus altered by mutation of DNA methyltransferase 3a and 3b显示文摘The epigenetic state of donor cells plays a vital role in the nuclear reprogramming and chromatin remodel-ing of cloned embryos.In this study we investigated the effect of DNA methylation state of donor cells on the development of mouse embryos reconstructed with embryonic stem(ES)cell nuclei.Our results confirmed that deletion of the DNA methyltransferase 3a(Dnmt3a)and DNA methyltransferase 3b(Dnmt3b)distinctly decreases the level of DNA methylation in ES cells.In contrast to wild type ES cells(J1),Dnmt3a–/–3b–/–(DKO)and Dnmt3b–/–(3bKO)donor cells significantly elevated the percentage of embryonic stem cell nuclear transfer(ECNT)morula,blastocysts and postimplantation embryos(P<0.05).However,the efficiency of establish-ment of NT-ES cell lines derived from DKO reconstructed blastocysts was not improved,and the expression pattern of OCT4 and CDX2 in cloned blastocysts and postim-plantation embryos was not altered either.Our results suggest that the DNA methylation state of the donor nucleus is an important factor in regulation of the donor nuclear reprogramming. | Xiangpeng DAI Xiaoyang ZHAO Hai TANG Jie HAO Jean-Paul RENARD Qi ZHOU Alice JOUNEAU Liu WANG | 2010 | Frontiers in Biology2010，5，5： | 0 |
| 9 | Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts显示文摘Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer (SCNT), holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells (hESCs) have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmission, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi-or pluripotency has not yet been evaluated. In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer- ESC (ntESC) lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results indicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning. | FAN Yong TONG Man ZHAO ChunLi DING ChenHui HAO Jie LV Zhuo DAI XiangPeng HAI Tang LI XueMei YAO RuQiang YU Yang LI ZanDong WANG Liu ALICE Jouneau ZHOU Qi | 2008 | Chinese Science Bulletin2008，53，23： | 0 |
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