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| 1 | The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato显示文摘GAMYBs are positive GA signaling factors that exhibit essential functions in reproductive development,particularly in anther and pollen development.However,there is no direct evidence of the regulation of any GAMYB in these biological processes in tomato(Solanum lycopersicum).Here,we identified a tomato GAMYB-like gene,SlMYB33,and characterized its specific roles.SlMYB33 is predominately expressed in the stamens and pistils.During flower development,high mRNA abundance of SlMYB33 is detected in both male and female organs,such as microspore mother cells,anthers,pollen grains,and ovules.Silencing of SlMYB33 leads to delayed flowering,aberrant pollen viability,and poor fertility in tomato.Histological analyses indicate that SlMYB33 exerts its function in pollen development in the mature stage.Further transcriptomic analyses imply that the knockdown of SlMYB33 significantly inhibits the expression of genes related to flowering in shoot apices,and alters the transcription of genes controlling sugar metabolism in anthers.Taken together,our study suggests that SlMYB33 regulates tomato flowering and pollen maturity,probably by modulating the expression of genes responsible for flowering and sugar metabolism,respectively. | Yan Zhang Bo Zhang Tongwen Yang Jie Zhang Bin Liu Xiangqiang Zhan Yan Liang | 2020 | Horticulture Research2020，7，1： | 5 |
| 2 | The tomato 2-oxoglutarate-dependent dioxygenase gene SlF3HL is critical for chilling stress tolerance显示文摘Low temperature is a major stress that severely affects plant development,growth,distribution,and productivity.Here,we examined the function of a 2-oxoglutarate-dependent dioxygenase-encoding gene,SlF3HL,in chilling stress responses in tomato(Solanum lycopersicum cv.Alisa Craig[AC]).Knockdown(KD)of SlF3HL(through RNA interference)in tomato led to increased sensitivity to chilling stress as indicated by elevated levels of electrolyte leakage,malondialdehyde(MDA)and reactive oxygen species(ROS).In addition,the KD plants had decreased levels of proline and decreased activities of peroxisome and superoxide dismutase.The expression of four cold-responsive genes was substantially reduced in the KD plants.Furthermore,seedling growth was significantly greater in AC or SlF3HLoverexpression plants than in the KD plants under either normal growth conditions with methyl jasmonate(MeJA)or chilling stress conditions.SlF3HL appears to positively regulate JA accumulation and the expression of JA biosynthetic and signaling genes under chilling stress.Together,these results suggest that SlF3HL is a positive regulator of chilling stress tolerance and functions in the chilling stress tolerance pathways,possibly by regulating JA biosynthesis,JA signaling,and ROS levels. | Tixu Hu Yuqin Wang Qiqi Wang Ningning Dang Ling Wang Chaochao Liu Jianhua Zhu Xiangqiang Zhan | 2019 | Horticulture Research2019，6，1： | 1 |
| 3 | The Epithelial-Mesenchymal Transition and Cancer Stem Cells: Functional and Mechanistic Links显示文摘 | Xiangqiang Liu Daiming Fan | 2015 | Current Pharmaceutical Design2015，，10： | 1 |
| 4 | Coaxial foilless diode显示文摘 | Kong Long Liu Qingxiang Li Xiangqiang | 2014 | AIP Advances2014，4，05： | 1 |
| 5 | A GW level high-power radial line helical array antenna 显示文摘 | LI Xiangqiang LIU Qingxiang Wu Xiaojiang | 2008 | IEEE trans on antennas and propagation2008，56，9： | 1 |
| 6 | A GW level high-power radial line helical array antenna显示文摘 | Li Xiangqiang Liu Qingxiang Wu Xiaojiang | 2008 | IEEE Trans on Antennas Pro pa g2008，56，9： | 1 |
| 7 | A GW level high-power radial line helical array antenna 显示文摘 | LI Xiangqiang LIU Qingxiang WU Xiaojiang ZHAO Liu | 2008 | IEEE trans on antennas and propagation2008，56，9： | 1 |
| 8 | Study of delayed neutron emission in slightly super-prompt-critical systemwith analog Monte Carlo method显示文摘 | Xie Qilin Fan Xiangqiang Liu Hangang | 2011 | Nucl Sci Eng2011，169，11： | 1 |
| 9 | Can Accounting Earnings Predict Future GDP Growth? Evidence from China显示文摘这研究调查财务收入是否能预言未来在瓷器机构的背景以内的粗野国内产品(GDP ) 生长。Konchitchki 和 Patatoukas (2014a ) 发现那项财务收入是 GDP 生长的重要的带指示物下一三或四个季度。然而,我们推测那个收入管理因为它弄歪,将为财务收入削弱如此的预兆的力量从真实社团的利润的收入。因为中国公司的收入是严重美国公司比那些操作的更多,这研究发现列出的公司能仅仅预言的汉语的那项收入 GDP 生长一个单个季度。我们进一步把财务收入分解成操作现金流动和获利收入并且发现是收入管理影响的更少的那操作现金流动在下一三个季度,而是获利收入的更长的地平线上为 GDP 有更好预兆的力量能仅仅预言 GDP 生长 1/4。 | Huacheng Wang Feng Cao Sifei Li Xiangqiang Liu | 2015 | Frontiers of Business Research in China2015，9，1： | 0 |
| 10 | Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311显示文摘The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type hybrids.Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type CMS.We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting rates.Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were predicted.Also,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene WA352.These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines. | Zuopeng Xu Yuanyue Du Xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang | 2023 | The Crop Journal2023，11，3： | 0 |
| 11 | Genetic analysis implicates a molecular chaperone complex in regulating epigenetic silencing of methylated genomic regions显示文摘DNA cytosine methylation confers stable epigenetic silencing in plants and many animals.However,the mechanisms underlying DNA methylation-mediated genomic silencing are not fully understood.We conducted a forward genetic screen for cellular factors required for the silencing of a heavily methylated p35S:NPTII transgene in the Arabidopsis thaliana rdm1-1 mutant background,which led to the identification of a Hsp20 family protein,RDS1(rdm1-1 suppressor 1).Loss-of-function mutations in RDS1 released the silencing of the p35S::NPTII transgene in rdm1-1 mutant plants,without changing the DNA methylation state of the transgene.Protein interaction analyses suggest that RDS1 exists in a protein complex consisting of the methyl-DNA binding domain proteins MBD5 and MBD6,two other Hsp20 family proteins,RDS2 and IDM3,a Hsp40/DNAJ family protein,and a Hsp70 family protein.Like rds1 mutations,mutations in RDS2,MBD5,or MBD6 release the silencing of the transgene in the rdm1 mutant background.Our results suggest that Hsp20,Hsp40,and Hsp70 proteins may form a complex that is recruited to some genomic regions with DNA methylation by methyl-DNA binding proteins to regulate the state of silencing of these regions. | Zhengyan Feng Xiangqiang Zhan Jia Pang Xue Liu Huiming Zhang Zhaobo Lang Jian-Kang Zhu | 2021 | Journal of Integrative Plant Biology2021，63，8： | 0 |
| 12 | RNA-seq profiling the transcriptome of secondary seed dormancy in canola(Brassica napus L.)显示文摘Secondary seed dormancy(SSD)is responsible for volunteer plants in canola fields,which causes a series of problems in canola production and serves as an important trait for the environmental safety assessment of transgenic canola.A canola cultivar with strong SSD was used to establish insight into seed transcriptomes in its secondarily dormant seeds and control seeds without dormancy by RNA-seq analysis,aiming to determine the molecular ecological characterizations of SSD.A dataset(more than 4 Gb)of valid sequences was obtained from each sample,which was combined to carry out the de novo assembling.The assembled sequences consisted of 314,261fragments with length[100 bp,including 29,740 long transcripts of length C 500 bp.Functional annotation indicated that 1,641 long transcripts could be categorized into 24 cluster of orthologous groups of proteins(COGs)and 16,515 transcripts were linked to 2,648 gene ontology(GO)terms.There were 452 long transcripts with significantly different expression identified by a threshold of[2-fold expression change(P\0.001)between samples,among which 343 transcripts were unambiguously homologous to Arabidopsis genes.The plant hormones abscisic acid and gibberellins were known as the pivotal regulators of seed dormancy and germination.Although genes responsible for either biosynthesis or signaling of each hormone could be widely verified from the SSD transcriptome,theirs expression evidences failed to correlate with the induction of SSD.Based on the enriched terms of gene ontology and KEGG orthology,as well as the expression models of candidate genes of SSD,we proposed that fatty acid metabolism might implicate in SSD in canola.The information reported here may play a significant role in further understanding of environmental safety assessment of SSD in transgenic canola. | Fuxia Liu Xiangqiang Zhao Lihua Zhang Tang Tang Changming Lu Guiming Chen Xinlong Wang Cuiping Bu Xiangxiang Zhao | 2014 | Chinese Science Bulletin2014，59，32： | 0 |
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