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| 1 | Olfactory ecto-mesenchymal stem cell-derived exosomes ameliorate murine Sjögren’s syndrome by modulating the function of myeloid-derived suppressor cells显示文摘Sjögren’s syndrome(SS)is a systemic autoimmune disease characterized by progressive inflammation and tissue damage in salivary glands and lacrimal glands.Our previous studies showed that myeloid-derived suppressor cells(MDSCs)exhibited impaired immunosuppressive function during disease progression in patients with SS and mice with experimental Sjögren’s syndrome(ESS),but it remains unclear whether restoring the function of MDSCs can effectively ameliorate the development of ESS.In this study,we found that murine olfactory ecto-mesenchymal stem cell-derived exosomes(OE-MSC-Exos)significantly enhanced the suppressive function of MDSCs by upregulating arginase expression and increasing ROS and NO levels.Moreover,treatment with OE-MSC-Exos via intravenous injection markedly attenuated disease progression and restored MDSC function in ESS mice.Mechanistically,OE-MSC-Exo-secreted IL-6 activated the Jak2/Stat3 pathway in MDSCs.In addition,the abundant S100A4 in OE-MSC-Exos acted as a key factor in mediating the endogenous production of IL-6 by MDSCs via TLR4 signaling,indicating an autocrine pathway of MDSC functional modulation by IL-6.Taken together,our results demonstrated that OE-MSC-Exos possess therapeutic potential to attenuate ESS progression by enhancing the immunosuppressive function of MDSCs,possibly constituting a new strategy for the treatment of Sjögren’s syndrome and other autoimmune diseases. | Ke Rui Yue Hong Qiugang Zhu Xiaofei Shi Fan Xiao Hailong Fu Qing Yin Yida Xing Xinfeng Wu Xiaodan Kong Huaxi Xu Jie Tian Shengjun Wang Liwei Lu | 2021 | Cellular & Molecular Immunology2021，18，2： | 12 |
| 2 | A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening显示文摘Ripening of the model fruit tomato(Solanum lycopersicum)is controlled by a transcription factor network including NAC(NAM,ATAF1/2,and CUC2)domain proteins such as No-ripening(NOR),SlNAC1,and SlNAC4,but very little is known about the NAC targets or how they regulate ripening.Here,we conducted a systematic search of fruit-expressed NAC genes and showed that silencing NOR-like1(Solyc07g063420)using virus-induced gene silencing(VIGS)inhibited specific aspects of ripening.Ripening initiation was delayed by 14 days when NOR-like1 function was inactivated by CRISPR/Cas9 and fruits showed obviously reduced ethylene production,retarded softening and chlorophyll loss,and reduced lycopene accumulation.RNA-sequencing profiling and gene promoter analysis suggested that genes involved in ethylene biosynthesis(SlACS2,SlACS4),color formation(SlGgpps2,SlSGR1),and cell wall metabolism(SlPG2a,SlPL,SlCEL2,and SlEXP1)are direct targets of NOR-like1.Electrophoretic mobility shift assays(EMSA),chromatin immunoprecipitation-quantitative PCR(ChIP-qPCR),and dual-luciferase reporter assay(DLR)confirmed that NOR-like1 bound to the promoters of these genes both in vitro and in vivo,and activated their expression.Our findings demonstrate that NOR-like1 is a new positive regulator of tomato fruit ripening,with an important role in the transcriptional regulatory network. | Ying Gao Wei Wei Xiaodan Zhao Xiaoli Tan Zhongqi Fan Yiping Zhang Yuan Jing Lanhuan Meng Benzhong Zhu Hongliang Zhu Jianye Chen Cai-Zhong Jiang Donald Grierson Yunbo Luo Da-Qi Fu | 2018 | Horticulture Research2018，5，1： | 9 |
| 3 | Effects of Plant Growth-Promoting Rhizobacteria and N Source on Plant Growth and N and P Uptake by Tomato Grown on Calcareous Soils显示文摘Introducing specific microorganisms into the soil ecological system is an important strategy for improving nutrient use efficiency.Two pot experiments were conducted in the greenhouse from December 3, 2012 to January 25, 2013(Experiment 1) and March 11 to April 23, 2013(Experiment 2) to evaluate the effect of nitrogen(N) source and inoculation with plant growth-promoting rhizobacteria(PGPR) on plant growth and N and phosphorus(P) uptake in tomato(Lycopersicon esculentum Mill.) grown on calcareous soils from South Florida, USA.Treatments included urea, controlled release urea(a controlled release fertilizer, CRF) each at low and high N rates and with or without inoculation of PGPR.A mixture of PGPR strains Bacillus amyloliquefaciens IN937 a and Bacillus pumilus T4 was applied to the soil during growing periods of tomato.Treatments with PGPR inoculation increased plant height compared to treatments without PGPR in both experiments.Inoculation with PGPR increased shoot dry weight and shoot N uptake for the same N rate and N source.In both experiments, only at high N rate, CRF and urea treatments with PGPR had significantly(P < 0.05)greater shoot biomass than those without PGPR.Only at high N rate, CRF treatment with PGPR significantly increased shoot N uptake by 39.0% and 10.3% compared to that without PGPR in Experiments 1 and 2, respectively.Meanwhile, presence of PGPR in the soil increased shoot P uptake for all treatments in Experiment 1 and for most treatments in Experiment 2.In Experiment 1, only at low N rate, CRF treatment with PGPR significantly increased shoot P uptake compared with that without PGPR.In Experiment2, a significant increase in shoot P uptake by inoculation of PGPR was only observed in CRF treatment at high N rate.Results from this study indicate that inoculation with PGPR may increase plant growth and N and P uptake by tomato grown on calcareous soils.However, the effect of PGPR varied and was influenced by many factors such as N source, N rate, and soil fertility.Further investigations are warranted to confirm the effect of PGPR under different soil conditions. | Xiaohui FAN Shouan ZHANG Xiaodan MO Yuncong LI Yuqing FU Zhiguang LIU | 2017 | Pedosphere2017，27，6： | 8 |
| 4 | Improvements of corner frequency and scaling factor for stochastic finite-fault modeling显示文摘In this paper, three existing source spectral models for stochastic finite-fault modeling of ground motion were reviewed. These three models were used to calculate the far-field received energy at a site from a vertical fault and the mean spectral ratio over 15 stations of the Northridge earthquake, and then compared. From the comparison, a necessary measure was observed to maintain the far-field received energy independent of subfault size and avoid overestimation of the long-period spectral level. Two improvements were made to one of the three models (i.e., the model based on dynamic corner frequency) as follows: (i) a new method to compute the subfault corner frequency was proposed, where the subfault corner frequency is determined based on a basic value calculated from the total seismic moment of the entire fault and an increment depending on the seismic moment assigned to the subfault; and (ii) the difference of the radiation energy from each subfault was considered into the scaling factor. The improved model was also compared with the unimproved model through the far-field received energy and the mean spectral ratio. The comparison proves that the improved model allows the received energy to be more independent of subfault size than the unimproved model, and decreases the overestimation degree of the long-period spectral amplitude. | Sun Xiaodan Tao Xiaxin Chen Fu | 2010 | Earthquake Engineering and Engineering Vibration2010，9，4： | 5 |
| 5 | Anti-oxidant and anti-inflammatory activities of ultrasonicassistant extracted polyphenol-rich compounds from Sargassum muticum显示文摘Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances. | YU Yuan WANG Le FU Xiaoting WANG Lei FU Xiaodan YANG Min HAN Zhenlian MOU Haijin JEON You-Jin | 2019 | Journal of Oceanology and Limnology2019，37，3： | 3 |
| 6 | Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds显示文摘Production of chitosan and its derivatives by traditional methods involves the excessive use of a reaction solution comprisedof sodium hydroxide and hydrochloric acid. Waste water resulting from this process has lim让ed the application of chitosanas a fertilizer as the process causes serious environmental pollution. Specifically, the resulting waste water contains highlevels of dissolved nitrogen and minerals from shrimp shells. In this study, an eco-friendly method was established to produce chitooligosaccharides (COS) with different degrees of deacetylation (DDAs) from shrimp shell waste. At a solid-tosolventratio of 1:6, the degree of demineralization was above 90% with the treatment of 30 g-L_1 H3PO4, and the degree ofdeproteinization was above 80% when treated with 30 g-L_1 KOH at 70 °C. Chitosans with different DDAs were obtainedby microwave-assisted KOH metathesis and the COS with Mw approximately 1500 Da were then prepared by oxidativedegradation. In summary, 33.73 kg H3PO4,12.77 kg, and 241.31 kg KOH were supplied during the processes of demineralization,deproteinization, and deacetylation of 100 kg shrimp shell waste, respectively. The process water was totally recycled,demonstrating that the shrimp shell could be wholly transformed into fertilize The entire process created a product withthe fractions of N:P2O5:K2O:COS = 7.94:24.44:10.72:1 &27. The test on the germination promotion of wheat seeds revealedthat the COS with 72」2% DDA significantly promoted germination. This work demonstrated the use of an eco-friendlypreparation method of COS with a specific degree of deacetylation that can be applied as a fertilizer. | Xiaodan Fu Lin Zhu Li Li Tan Zhang Meng Li Haijin Mou | 2019 | Marine Life Science & Technology2019，1，1： | 2 |
| 7 | Bacteriostatic effect of lipopeptides from Bacillus subtilis N-2 on Pseudomonas putida using soybean meal by solid-state fermentation显示文摘Bacillus subtilis N-2 which was isolated from natto,produced lipopeptides using soybean meal as a substrate.This work aimed to purify,identify,and determine the antibacterial mechanism of lipopeptides produced by B.subtilis N-2.The fermented product obtained by solid-state fermentation was subjected to water extraction,acid precipitation,and methanol extraction.Fractions were separated and collected using a two-step ultrafiltration method and then identified by LC-MS/MS.Mass spectrometry characterization revealed the presence of four variants of iturin A that differed according to the p-amino fatty acid chain from C14 to C17 as well as the amino acid positions.A new lipopeptide(m/z 1070.3)was identified and its structure was different from the previously reported lipopeptides.The lipopeptides were shown to inhibit the growth of an isolate of Pseudomonas putida,a common pathogen in decaying fish,by changing membrane permeability.These results suggest that the lipopeptides from B.subtilis N-2 could be used as a biocontrol agent in aquaculture. | Meng Li Haijin Mou Qing Kong Tan Zhang Xiaodan Fu | 2020 | Marine Life Science & Technology2020，2，2： | 1 |
| 8 | Adsorption characteristics of Ni(II) onto MA–DTPA/PVDF chelating membrane显示文摘 | Xiaodan Zhao Laizhou Song Jie Fu Pei Tang Feng Liu | 2011 | Journal of Hazardous Materials2011，，3： | 1 |
| 9 | Effect of seasonal snow on the start of growing season of typical vegetation in Northern Hemisphere显示文摘Under global warming, seasonal snow takes faster melting rate than before, which greatly changes the hydro-logical cycle. In this study, by targeting three typical seasonal snow-covered land types (i.e., open shrubland,evergreen needleleaf forest and mixed forest) in the Northern Hemisphere, the start of growing season (SGS) hasbeen found obviously advanced in the past years, greatly contributed by the faster melting rate of seasonal snow.It is manifested that significantly positive correlation has been found between SGS and May snow depth for openshrubs, March and April snow depth for evergreen needleleaf forests and March snow depth for mixed forests.However, such close association is not appeared in all the climate conditions of same vegetation. In the future,as the rate of melting snow becomes faster in the high emission of greenhouse gasses than the current situation,continuously advanced SGS will accelerate the change of vegetation distribution in the Northern Hemisphere.These findings offer insights into understanding the effect from seasonal snow on vegetation and promote thesustainable utilization of regional vegetation in the Northern Hemisphere. | Xiaodan Guan Shuyang Guo Jianping Huang Xiaohan Shen Li Fu Guolong Zhang | 2022 | Geography and Sustainability2022，3，3： | 0 |
| 10 | 缰核相关神经通路可介导光疗的抗抑郁作用显示文摘文章简介视网膜所感知的光信息可调节抑郁情绪在内的多种非成像功能。虽然临床研究表明光疗可显著改善抑郁症状,但光疗抗抑郁作用产生的神经通路机制尚不明确。在本研究中,研究人员通过采用神经环路示踪技术发现在小鼠中存有一条连接视网膜(Retina)与外侧缰核(LHb)的跨双突触神经通路。 | 黄鲁 席月 黄晓丹 Yan Yang Xiaodan Huang Yunwei Fu Qian Tao Jia Xiao Tifei Yuan Kai An Huan Zhao Mingliang Pu Fuqiang Xu Tian Xue Minmin Luo Kwok-Fai So 任超然 苏国辉 | 2020 | 科学新闻2020，，2： | 0 |
| 11 | Inhibition of HIPK3 by AST487 Ameliorates Mutant HTT-Induced Neurotoxicity and Apoptosis via Enhanced Autophagy显示文摘Dear Editor,Accumulation of misfolded and aggregation-prone proteins is the common hallmark of many neurodegenerative disorders,and lowering the levels of these proteins may provide promising strategies for the potential treatment of some of these diseases[1,2].Among them,Huntington’s disease(HD)is a monogenic disease caused by mutation of the HTT(huntingtin)gene[3],which encodes the mutant HTT protein(mHTT)with an expanded polyglutamine tract(polyQ). | Xiaodan Zhang Xue Wen Ismael Al-Ramahi Juan Botas Boxun Lu Yuhua Fu | 2022 | Neuroscience Bulletin2022，38，1： | 0 |
| 12 | Model Predictive Control for Cascaded H-Bridge PV Inverter with Capacitor Voltage Balance显示文摘We designed an improved direct-current capacitor voltage balancing control model predictive control(MPC)for single-phase cascaded H-bridge multilevel photovoltaic(PV)inverters.Compared with conventional voltage balanc-ing control methods,the method proposed could make the PV strings of each submodule operate at their maximum power point by independent capacitor voltage control.Besides,the predicted and reference value of the grid-connected current was obtained according to the maximum power output of the maximum power point tracking.A cost function was con-structed to achieve the high-precision grid-connected control of the CHB inverter.Finally,the effectiveness of the proposed control method was verified through a semi-physical simulation platform with three submodules. | Xinwei Wei Wanyu Tao Xunbo Fu Xiufeng Hua Zhi Zhang Xiaodan Zhao Chen Qin | 2024 | Journal of Electronic Research and Application2024，8，2： | 0 |
| 13 | Correction:Inhibition of HIPK3 by AST487 Ameliorates Mutant HTT-Induced Neurotoxicity and Apoptosis via Enhanced Autophagy显示文摘We are sorry that we have made a few minor errors regarding the description of the scale bars and data presentation in the figure legends(not the figures)of the following manuscript:We’d like to make the following corrections:The authors would like to correct the following errors in the figure legends of Fig.1B–1D and Fig.2F–2I by changing“mean±SEM”to“mean±SD”. | Xiaodan Zhang Xue Wen Ismael Al-Ramahi Juan Botas Boxun Lu Yuhua Fu | 2022 | Neuroscience Bulletin2022，38，9： | 0 |
| 14 | Response of Salmonella enterica serovar Typhimurium to alginate oligosaccharides fermented with fecal inoculum:integrated transcriptomic and metabolomic analyses显示文摘Alginate oligosaccharides(AOS),extracted from marine brown algae,are a common functional feed additive;however,it remains unclear whether they modulate the gut microbiota and microbial metabolites.The response of Salmonella enterica serovar Typhimurium,a common poultry pathogen,to AOS fermented with chicken fecal inocula was investigated using metabolomic and transcriptomic analyses.Single-strain cultivation tests showed that AOS did not directly inhibit the growth of S.Typhimurium.However,when AOS were fermented by chicken fecal microbiota,the supernatant of fermented AOS(F-AOS)exhibited remarkable antibacterial activity against S.Typhimurium,decreasing the abundance ratio of S.Typhimurium in the fecal microbiota from 18.94 to 2.94%.Transcriptomic analyses showed that the 855 diferentially expressed genes induced by F-AOS were mainly enriched in porphyrin and chlorophyll metabolism,oxidative phosphorylation,and Salmonella infection-related pathways.RT-qPCR confrmed that F-AOS downregulated key genes involved in fagellar assembly and the type III secretory system of S.Typhimurium,indicating metabolites in F-AOS can infuence the growth and metabolism of S.Typhimurium.Metabolomic analyses showed that 205 microbial metabolites were signifcantly altered in F-AOS.Among them,the increase in indolelactic acid and 3-indolepropionic acid levels were further confrmed using HPLC.This study provides a new perspective for the application of AOS as a feed additive against pathogenic intestinal bacteria. | Jiaying Cheng Mengshi Xiao Xinmiao Ren Francesco Secundo Ying Yu Shihao Nan Weimiao Chen Changliang Zhu Qing Kong Youtao Huang Xiaodan Fu Haijin Mou | 2023 | Marine Life Science & Technology2023，5，2： | 0 |
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