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| 1 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 2 | Chinese expert consensus statement on the diagnosis and treatment of fulminant myocarditis显示文摘Fulminant myocarditis is primarily caused by infection with any number of a variety of viruses. It arises quickly, progresses rapidly, and may lead to severe heart failure or circulatory failure presenting as rapid-onset hypotension and cardiogenic shock,with mortality rates as high as 50%–70%. Most importantly, there are no treatment options, guidelines or an expert consensus statement. Here, we provide the first expert consensus, the Chinese Society of Cardiology Expert Consensus Statement on the Diagnosis and Treatment of Fulminant Myocarditis, based on data from our recent clinical trial(NCT03268642). In this statement, we describe the clinical features and diagnostic criteria of fulminant myocarditis, and importantly, for the first time,we describe a new treatment regimen termed life support-based comprehensive treatment regimen. The core content of this treatment regimen includes(i) mechanical life support(applications of mechanical respirators and circulatory support systems,including intraaortic balloon pump and extracorporeal membrane oxygenation),(ii) immunological modulation by using sufficient doses of glucocorticoid, immunoglobulin and(iii) antiviral reagents using neuraminidase inhibitor. The proper application of this treatment regimen may and has helped to save the lives of many patients with fulminant myocarditis. | Daowen Wang Sheng Li Jiangang Jiang Jiangtao Yan Chunxia Zhao Yan Wang Yexin Ma Hesong Zeng Xiaomei Guo Hong Wang Jiarong Tang Houjuan Zuo Li Lin Guanglin Cui Section of Precision Medicine Group of Chinese Society of Cardiology,Editorial Board of Chinese Journal of Cardiology &Working Group of Adult Fulminant Myocarditis | 2019 | Science China(Life Sciences)2019，62，2： | 18 |
| 3 | External Characteristics and Internal Flow Features of a Centrifugal Pump during Rapid Startup显示文摘Centrifugal pumps always work under steady conditions,and many researches focus on the steady operation.But transient conditions,such as sudden startup and shutdown,are inevitable.The researches on the inner flow of centrifugal pumps under transient conditions have been done,and they show that the transient operation is different from the steady operation.In order to research the evolution of unsteady flow in a centrifugal pump under transient conditions,and to investigate the mechanism of transient effects by analyzing the unsteady flow in a centrifugal pump,the external characteristic experiment and the internal flow numerical calculation of the centrifugal pump with an open impeller during startup is presented.The relationships of the rotation speed,capacity and head between start-time are obtained by the external characteristics experiment.The numerical calculations under startup process are carried out by using the k-e model and N-S equation.The distribution of velocity and pressure in the inner channel of the tested pump was obtained by choosing fourteen start-time points and twelve geometrical points in the impeller channel during startup.The calculation results show that the velocity and the pressure increase linearly with the start-time before rotation's speed gets steady,then changes almost horizontally after rotation speed becomes steady,then fluctuates until being steady.The internal flow characteristics are in good agreement with the external characteristic experimental results and numerical calculation.The simulation methods and results make the basis for the diagnosis and optimization of under flow in the centrifugal pump during transient operation. | ZHU Zuchao GUO Xiaomei CUI Baoling LI Yi | 2011 | Chinese Journal of Mechanical Engineering2011，24，5： | 14 |
| 4 | miR-188-5p regulates proliferation and invasion via PI3K/Akt/MMP-2/9 signaling in keloids显示文摘Keloids(KDs)and hypertrophic scars(HSs),two forms of pathological scars,seriously affect the physical and psychological health of patients.Despite many similarities with HSs,KDs are characterized by invasion and a high rate of recurrence after surgery,features they share in common with tumors.The underlying molecular mechanisms of this phenomenon have not been fully elucidated.In this study,we used microRNA(miRNA)array analysis to search for invasion-associated miRNAs in KDs.The expression of miR-188-5p in KDs,HSs,normal skin(NS)tissues,and cell lines was measured by quantitative real-time polymerase chain reaction.Furthermore,cell proliferation,migration,and invasion were detected in KD fibroblasts(KFs)and HS fibroblasts(HSFs),and interrelated proteins were ascertained by western blot analysis.It was found that miR-188-5p was significantly decreased in KD tissue compared with HS and NS tissues.Upregulated expression of miR-188-5p suppressed KF proliferation,migration,and invasion;and decreased expression of miR-188-5p also promoted HSF proliferation,migration,and invasion.The protein levels of MMP-2,MMP-9,PI3K,and p-Akt in miR-188-5p mimic-transfected KFs were repressed.In contrast,after transfection with miR-188-5p inhibitor,the protein levels of MMP-2,MMP-9,PI3K,and p-Akt were higher than the control in HSFs.Treatment with PI3K/Akt inhibitor LY294002 in KFs with miR-188-5p inhibitor did not further reduce their proliferation,migration,and invasion.The upregulation of MMP-2 and MMP-9 by miR-188-5p inhibitor could be abolished by LY294002.These findings together demonstrate a tumor-suppressive role of miR-188-5p in KD proliferation and invasion via PI3K/Akt/MMP-2/9 signaling,indicating that miR-188-5p may be a potential prognostic marker and therapeutic target for KDs. | Wenyan Zhu Xiaoyan Wu Bo Yang Xiaodong Yao Xiaomei Cui Pan Xu Xiaodong Chen | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 9 |
| 5 | Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites显示文摘MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions. | WANG Ketao SU Xiaomei CUI Xia DU Yongchen ZHANG Shuaibin GAO Jianchang | 2018 | Horticultural Plant Journal2018，4，2： | 6 |
| 6 | Molecular characteristics and evolutionary analysis of a very virulent infectious bursal disease virus显示文摘Infectious bursal disease virus(IBDV) poses a significant threat to the poultry industry. Viral protein 2(VP2), the major structural protein of IBDV, has been subjected to frequent mutations that have imparted tremendous genetic diversity to the virus. To determine how amino acid mutations may affect the virulence of IBDV, we built a structural model of VP2 of a very virulent strain of IBDV identified in China, vv IBDV Gx, and performed a molecular dynamics simulation of the interaction between virulence sites. The study showed that the amino acid substitutions that distinguish vv IBDV from attenuated IBDV(H253Q and T284A) favor a hydrophobic and flexible conformation of ?-barrel loops in VP2, which could promote interactions between the virus and potential IBDV-specific receptors. Population sequence analysis revealed that the IBDV strains prevalent in East Asia show a significant signal of positive selection at virulence sites 253 and 284. In addition, a signal of co-evolution between sites 253 and 284 was identified. These results suggest that changes in the virulence of IBDV may result from both the interaction and the co-evolution of multiple amino acid substitutions at virulence sites. | LI Zan QI XiaoLe REN XianGang CUI Lei WANG XiaoMei ZHU Ping | 2015 | Science China(Life Sciences)2015，58，8： | 6 |
| 7 | Alterations in gut microbiota and metabolites associated with altitude-induced cardiac hypertrophy in rats during hypobaric hypoxia challenge显示文摘The gut microbiota is involved in host responses to high altitude.However,the dynamics of intestinal microecology and their association with altitude-related illness are poorly understood.Here,we used a rat model of hypobaric hypoxia challenge to mimic plateau exposure and monitored the gut microbiome,short-chain fatty acids(SCFAs),and bile acids(BAs)over 28 d.We identified weight loss,polycythemia,and pathological cardiac hypertrophy in hypoxic rats,accompanied by a large compositional shift in the gut microbiota,which is mainly driven by the bacterial families of Prevotellaceae,Porphyromonadaceae,and Streptococcaceae.The aberrant gut microbiota was characterized by increased abundance of the Parabacteroides,Alistipes,and Lactococcus genera and a larger Bacteroides to Prevotella ratio.Trans-omics analyses showed that the gut microbiome was significantly correlated with the metabolic abnormalities of SCFAs and BAs in feces,suggesting an interaction network remodeling of the microbiome-metabolome after the hypobaric hypoxia challenge.Interestingly,the transplantation of fecal microbiota significantly increased the diversity of the gut microbiota,partially inhibited the increased abundance of the Bacteroides and Alistipes genera,restored the decrease of plasma propionate,and moderately ameliorated cardiac hypertrophy in hypoxic rats.Our results provide an insight into the longitudinal changes in intestinal microecology during the hypobaric hypoxia challenge.Abnormalities in the gut microbiota and microbial metabolites contribute to the development of high-altitude heart disease in rats. | Zhiyuan Pan Yichen Hu Zongyu Huang Ni Han Yan Li Xiaomei Zhuang Jiye Yin Hui Peng Quansheng Gao Wenpeng Zhang Yong Huang Yujun Cui Yujing Bi Zhenjiang Zech Xu Ruifu Yang | 2022 | Science China(Life Sciences)2022，65，10： | 5 |
| 8 | Anti-cavitation performance of a splitter-bladed inducer under different flow rates and different inlet pressures显示文摘The anti-cavitation performance of a high-speed centrifugal pump with a splitter-bladed inducer is investigated under different flow rates and different inlet pressures. Simulations and external characteristics experiments are carried out. Static pressure and the vapor volume fraction distributions on the inducer and the impeller of the pump under various operation conditions are obtained. The results show that the cavitation developments on the impeller and on the inducer with the flow rates are reverse, while the development of the inlet pressure on the inducer and the impeller is the same. Cavitation on the impeller increases with the increase of flow rates, and it extends to the near passages with rotating, while cavitation on the inducer is more complex than that on the impeller. Cavitation at the inlet of the inducer decreases with the increase of flow rates, while cavitation at the outlet of the inducer is opposite. The results also show that cavitation development on the impeller and on the inducer with the inlet pressure is the same. Cavitation both decreases with the increase of the inlet pressure at the same flow rate. Furthermore, asymmetric cavitation on the impeller and on the inducer is both observed. And the asymmetric degree of cavitation on the impeller is higher than that on the inducer. | GUO XiaoMei ZHU ZuChao CUI BaoLing HUANG Yong | 2015 | Science China(Technological Sciences)2015，58，12： | 4 |
| 9 | A single mutation in the PBC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus显示文摘To test whether amino acid mutations in the PBC and PHI loops of VP2 are involved in the replication and virulence of infectious bursal disease virus(IBDV), a pair of viruses, namely the moderately virulent IBDV(rG x-F9VP2) and the attenuated strain(rGt), were used. Residue mutations A222P(P_(BC)) and S330R(PHI), selected by sequence comparison, were introduced individually into r Gx-F9VP2 by using a reverse genetics system. In addition, the reverse mutation of either P222 A or R330 S was introduced into r Gt. The four modified viruses were then rescued and evaluated in vitro(CEF cells) and in vivo(SPF chickens). Results showed that A222 P elevated the replication efficiency of rG x-F9VP2 while P222 A reduced that of rG t in CEF cells. A mutation at residue 330 did not alter IBDV replication. In addition, animal experiments showed that a single mutation at either residue 222 or 330 did not significantly influence the virulence of IBDV. In conclusion, residue 222 in PBC of VP2 is involved in the replication efficiency of IBDV in vitro but does not affect its virulence in vivo, further facilitating our understanding of the gene-function of IBDV. | Xiaole Qi Xiang Gao Zhen Lu Lizhou Zhang Yongqiang Wang Li Gao Yulong Gao Kai Li Honglei Gao Changjun Liu Hongyu Cui Yanping Zhanga Xiaomei Wang | 2016 | Science China(Life Sciences)2016，59，7： | 2 |
| 10 | Experimental and numerical investigations on fatigue behavior of aluminum alloy 7050-T7451 single lap four-bolted joints显示文摘The fatigue behavior of aluminum alloy 7050-T7451 single lap four-bolted joints was studied by highfrequency fatigue test and finite element(FE) methods. The fatigue test results showed that a better enhancement of fatigue life was achieved for the joints with high-locked bolts by employing the combinations of cold expansion, interference fit, and clamping force. The fractography revealed that fatigue cracks propagated tortuously; more fatigue micro-cliffs, tearing ridges, lamellar structure were observed,and fatigue striation spacing was simultaneously reduced. The evaluation of residual stress conducted by FE methods confirmed the experimental results and locations of fatigue crack initiation. The extension of fatigue lives can be attributed to the evolution of fatigue damage and effect of beneficial compressive residual stresses around the hole, resulting in the delay of crack initiation, crack deflection, and plasticity-induced crack closure. | Xiaomei Liu Hao Cui Shangzhou Zhang Haipo Liu Gaofeng Liu Shujun Li | 2018 | Journal of Materials Science & Technology2018，34，7： | 2 |
| 11 | Cascades in small-world modular networks with CMLS method显示文摘 | CUI DI GAO ZIYOU ZHAO XIAOMEI | 2007 | Modern Physics Letters B2007，21，30： | 1 |
| 12 | Different Deswelling Behavior of Temperature-Sensitive Microgels of Poly (N-Isopropylacrylamide) Crosslinked by Polyethylene-Glycol Dimethacrylates显示文摘 | Ma Xiaomei Cui Yanjun Zhao Xian | 2004 | J Colloid Interface Sci2004，276，1： | 1 |
| 13 | Suppression of matrix metalloproteinase-9 expression by RNA interference inhibits SGC7901 gastric adenocarcinoma cell growth and invasion in vitro and in vivo显示文摘 | Fengjuan Zhao Qingyu Zhang Chunsheng Kang Xiaowei Cui Tao Wang Peng Xu Xuan Zhou Jian Liu Xiaomei Song | 2010 | Medical Oncology2010，，3： | 1 |
| 14 | Synthesis of didodecylmethylcarboxyl betaine and its application in surfactant - polymer flooding 显示文摘 | CUI ZHENGGANG DU XIANGRUI PEI XIAOMEI | 2012 | Journal of Surfactants and Deterfents2012，15，6： | 1 |
| 15 | Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects显示文摘Injectable bone biomaterials like bone cement should be designed and fabricated with certain biological criteria,which include:1)recruitment and polarization of the macrophages from M1(pro-inflammatory)to M2(anti-inflammatory)phenotype,2)enhance vascularization,and 3)activate osteogenic differentiation of bone marrow-derived stem cells to promote bone healing.So far,no injectable biomaterials could spontaneously regulate the entire bone healing process that involves inflammation,angiogenesis,and osteogenesis.Therefore,in this study,we designed bone cement comprised of strontium and copper-incorporated borosilicate glass(Sr/Cu-BSG)in the liquid phase of chitosan to modulate bone healing.In vitro studies showed that the controlled release of Sr and Cu ions up-regulated anti-inflammatory genes(IL-1Ra and TGF-β1)while down-regulating pro-inflammatory genes(IL-1βand IL-6)in macrophages at 3 days.Sr and Cu ions also increased the expressions of angiogenic genes(VEGF and bFGF)in HUVECs at 5 days and osteogenic genes(Runx-2,OCN,and OPN)in hBMSCs at 7,14,and 21 days.5Sr3Cu-BSG bone cement exhibited the best anti-inflammatory,angiogenic,and osteogenic properties among the bone cement groups with different Sr and Cu ratios.Short-term and long-term implantation of Sr/Cu-BSGs in femoral condylar bone defects of rats and rabbits confirmed the in vitro results,where the degradation rate of Sr/Cu-BSG matched the bone healing rate.Similar to in vitro,the 5Sr3Cu-BSG group also showed the highest bone formation in vivo.Excellent physical and chemical properties,along with its bone repairing ability,make the Sr/Cu-BSG bone cement a good candidate biomaterial for treating bone defects. | Shuaijie Li Liyan Zhang Chunyu Liu Jua Kim Kun Su Tingli Chen Limin Zhao Xiaomei Lu Hao Zhang Yinglin Cui Xu Cui Feng Yuan Haobo Pan | 2023 | Bioactive Materials2023，，5： | 1 |
| 16 | Interactions Between Thrombin with Flavonoids from Abelmoschus manihot (L.) Medicus by CZE显示文摘 | Yi Liu Xianyin Lai Xiaomei Ling Yuying Zhao Jingrong Cui | 2006 | Chromatographia (-)2006，，1： | 1 |
| 17 | Synthesis, characterization, and thermal stability of star-shaped poly(ε-caprolactone) with phosphazene core显示文摘 | Yanjun Cui Xiaomei Ma Xiaozhen Tang Yinpei Luo | 2003 | European Polymer Journal2003，，2： | 1 |
| 18 | Prevalence Characteristics of Rice Black-streaked Dwarf Virus Disease and Continuous Control Strategies显示文摘Through summarizing the prevalence characteristics of rice black-streaked dwarf virus disease(RBSDVD)in Linyi City of Shandong Province,this paper analyzed its prevalence is related to changes in farming and cultivation systems,the increase in the population of venomous Laodelphax striatellus Fallén and its own migration and spread,the poor disease resistance of cultivated varieties,and inadequate time of prevention and control.Besides,based on the practice of local control,it came up with some comprehensive control measures including strengthening monitoring,early warning and forecasting,planting resistant(tolerant)rice varieties according to local conditions,appropriately delaying the sowing(planting)period,supplemented by insect nets to cover seedlings,and making scientific use of chemical control.It is expected to provide a reference for the prevention and control of RBSDVD. | Deyou LIU Yangang LIU Lijuan LIU Xiaomei CUI Zhaotang SHEN Jinqian LIU Xiugang YU Zhouliang WANG Shengfeng ZHANG Qingquan MU Xiushan ZHAO | 2021 | Asian Agricultural Research2021，13，6： | 0 |
| 19 | Corrigendum to: miR-188-5p regulates proliferation and invasion via PI3K/Akt/MMP-2/9 signaling in keloids显示文摘 | Wenyan Zhu Xiaoyan Wu Bo Yang Xiaodong Yao Xiaomei Cui Pan Xu Xiaodong Chen | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 0 |
| 20 | Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan显示文摘Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However. | Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi | 2019 | Virologica Sinica2019，34，1： | 0 |
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