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| 1 | A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress显示文摘Heterografting has long been used to enhance the chilling tolerance of temperature-sensitive crops,including watermelon,whose mechanism is known to involve bidirectional long-distance mRNA movements.Despite several studies reporting on mobile mRNA(mb-mRNA)profiles in plants,accurate identification of mb-mRNAs is challenging owing to an array of technical problems.Here,we developed a bioinformatical pipeline that took most of the known technical concerns into consideration and is considered to be a universal tool for mb-mRNA detection in heterografts.By applying this pipeline to a commercial watermelon–bottle gourd heterografting system,we detected 130 and 1144 mb-mRNAs upwardly and 167 and 1051 mb-mRNAs downwardly transmitted under normal and chilling-stress conditions,respectively.Quantitative real-time PCR indicated a high accuracy rate(88.2%)of mb-mRNA prediction with our pipeline.We further revealed that the mobility of mRNAs was not associated with their abundance.Functional annotation and classification implied that scions may convey the stress signal to the rootstock,subsequently triggering energy metabolism reprogramming and abscisic acid-mediated stress responses by upward movement of effective mRNAs,ultimately leading to enhanced chilling tolerance.This study provides a universal tool for mb-mRNA detection in plant heterografting systems and novel insights into heterografting advantages under chilling stress. | Ying Wang Lingping Wang Nailin Xing Xiaohua Wu Xinyi Wu Baogen Wang Zhongfu Lu Pei Xu Ye Tao Guojing Li Yuhong Wang | 2020 | Horticulture Research2020，7，1： | 3 |
| 2 | Description of two new species of Newsteadia Green(Hemiptera:Coccomorpha:Ortheziidae)from China with a key to Chinese species显示文摘This study reported two new species:Newsteadia multipori Zheng&Wu,sp.nov.,collected from pine needle litter in Guizhou Province,are described and illustrated for its adult female,male,and first-,second-and third-instar nymphs;N.chebalingensis Zheng&Wu,sp.nov.,collected from broad leaf litter in Guangdong Province,are described and illustrated for its adult female and first-instar nymph.Identification keys are provided to the adult females of Newsteadia species known in China and to the adult males of Newsteadia species known worldwide. | Xinyi Zheng San’an Wu Jichun Xing | 2023 | Zoological Systematics2023，48，3： | 0 |
| 3 | Clinical Efficacy of the Integrated Medical Care Model Combined with Psychological Intervention in Tuberculosis Patients with Lung Cancer显示文摘Objective:To explore the clinical efficacy of the integrated medical care model combined with psychological intervention in pulmonary tuberculosis patients with lung cancer.Methods:From January to December 2022,60 pulmonary tuberculosis patients with lung cancer admitted to our hospital were selected as the research subjects.Using the random number table method,the patients were divided into two groups,a control group and a study group,with 30 cases in each group.The chest computed tomography(CT)examination results,mental state assessment(including depression scale and anxiety scale)scores,incidence of adverse reactions,treatment effect,and length of hospital stay were compared between the two groups.Results:The treatment effect of the patients in the study group was better than that of the patients in the control group(P<0.05);the duration of hospitalization,chest CT examination results,mental state assessment scores,and incidence of adverse reactions of the study group and the control group,were significantly different(P<0.05).Conclusion:The integrated medical care model combined with psychological intervention can effectively improve the treatment effect of pulmonary tuberculosis patients with lung cancer and prevent the occurrence of adverse reactions;thus,it should be promoted in clinical practice. | Wei Yuan Dandan Yang Xinyi Ye Zhangying Li Xing Luan Min Zheng Yanling Feng Mi Zhang Chuo Guo | 2023 | Journal of Clinical and Nursing Research2023，7，2： | 0 |
| 4 | Seasonal Variation of the Sea Surface Temperature Growth Rate of ENSO显示文摘El Ni?o–Southern Oscillation(ENSO) exhibits a distinctive phase-locking characteristic, first expressed during its onset in boreal spring, developing during summer and autumn, reaching its peak towards winter, and decaying over the next spring. Several studies have demonstrated that this feature arises as a result of seasonal variation in the growth rate of ENSO as expressed by the sea surface temperature(SST). The bias towards simulating the phase locking of ENSO by many state-of-the-art climate models is also attributed to the unrealistic depiction of the growth rate. In this study, the seasonal variation of SST growth rate in the Ni?o-3.4 region(5°S–5°N, 120°–170°W) is estimated in detail based on the mixed layer heat budget equation and recharge oscillator model during 1981–2020. It is suggested that the consideration of a variable mixed layer depth is essential to its diagnostic process. The estimated growth rate has a remarkable seasonal cycle with minimum rates occurring in spring and maximum rates evident in autumn. More specifically, the growth rate derived from the meridional advection(surface heat flux) is positive(negative) throughout the year. Vertical diffusion generally makes a negative contribution to the evolution of growth rate and the magnitude of vertical entrainment represents the smallest contributor. Analysis indicates that the zonal advective feedback is regulated by the meridional immigration of the intertropical convergence zone, which approaches its southernmost extent in February and progresses to its northernmost location in September, and dominates the seasonal variation of the SST growth rate. | Xinyi XING Xianghui FANG Da PANG Chaopeng JI | 2024 | Advances in Atmospheric Sciences2024，41，3： | 0 |
| 5 | A novel lysosome‐localized fluorescent probe with aggregation‐induced emission without alkalinizing effect显示文摘Herein,a novel small molecule probe,tetraphenylethylene‐cystein(TPE‐Cys),was rationally designed and developed for the intracellular lysosome localization.The combination of tetraphenylethylene and cysteine serve as a novel strategy for lysosome‐targeting,with significant aggregation‐induced emission increases with the proportion of water by as high as 25‐folds.Biofluorescence imaging experiments show that the probe TPE‐Cys has a good colocalization effect with the commercially available lysotracker red,with a Pearson correlation coefficient of 0.91.In addition,we also demonstrate that TPE‐Cys has good light stability.TPE‐Cys,as a neutral compound,is free of alkalinizing effect when applied in lysosome localization.Moreover,molecular dynamics simulations and density function theories are employed to explore the detailed aggregation process and the mechanism of TPE‐Cys aggregation in water solution.The mechanism of lysosome localization of probe TPE‐Cys was explained by cellular endocytosis process and the formation of larger particle in the more acidic environment of lysosomes.This work not only demonstrates an efficient strategy for the construction of fluorescent probes for lysosome localization,but also indicates that they are promising as live cell imaging tools. | Jingran Zhang Yan Jia Xinyi Xing Mengmei Qin Zibo Wu Yingqian Zhong Lele Liu Shuqing Sun Peng Li Haiyuan Wang Guangjiu Zhao | 2021 | SmartMat2021，2，4： | 0 |
| 6 | Correction to:A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress显示文摘After the publication of this article1,the authors became aware that there was an error in Fig.2b that“Ref2-M”(below“Bowtie2”)on the right panel was mistakenly written as“Ref1-M”.The correct version is shown below. | Ying Wang Lingping Wang Nailin Xing Xiaohua Wu Xinyi Wu Baogen Wang Zhongfu Lu Pei Xu Ye Tao Guojing Li Yuhong Wang | 2020 | Horticulture Research2020，7，1： | 0 |
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