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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | Ecological compensation based on willingness to accept for conservation of drinking water sources显示文摘生态的赔偿是为在喝的水消息提供者被找到的区域的环境保护的一个强大的环境经济工具。我们基于回答者心甘情愿建立了一个生态的赔偿财务系统接受(WTA ) 。在这个系统,影响他们的股东偏爱和因素能用 logit 和 tobit 模型有效地被计量。我们为 Miyun 水库把这个财务系统用于生态的赔偿,中国北京。为 Miyun 水库居民的平均 WTA 价值比本地保护花费的每在 2012 的家庭是约 1186 CNY,它能被放作为一个合适的赔偿标准,自从它稍微高。因此,年度全部的生态的赔偿能是 5873 万 CNY。从到环境保护的水库,工作类型,和态度的距离是有 WTA 上的重要效果的变量。另外,为单个偏爱的趋势经由关键有影响的因素的分析被识别。结果为证实为喝的保存的生态的赔偿机制浇来源建议一些有用信息。建议包括在居民之中推广生态的赔偿的概念和意思,设置基于从水库的距离的不同赔偿层次,并且拿,就农民的要求而言各种各样在里面客气并且 out-of-kind 赔偿途径。 | Linyu XU Bing YU Yang LI | 2015 | Frontiers of Environmental Science & Engineering2015，9，1： | 3 |
| 3 | Research and application of schemes for constructing concrete pillars in large section finishing cut in backfill coal mining显示文摘Based on the technology of controlling surrounding rock deformation by constructing concrete pillars in large section finishing cut in backfill coal mining, the characteristics of vertical stress on concrete pillars and main factors influencing pillar stability are analyzed. By building a Winkler elastic foundation mechanical model for the support system constituted of coal pillar, backfill body and concrete pillars,mechanical calculation on stability of concrete pillar is carried out to evaluate the pillar stability and safety. Seven numeral models in three schemes with different pillar sizes, inter-row distances and compression ratios at the stopes were analyzed through numerical simulation according to width reduction principle. The practice of finishing cut at III644 workface at Yangzhuang coal mine shows that: when the actual compression ratio is 86.5%, construction size inside the finishing cut is 2000 mm 2000 mm and the interval between concrete pillars is 2000 mm 2000 mm, the pillars can be stable with the maximum movement of two sides of each pillar being only 83 mm and 54 mm, which achieves the expected effect. | Sun Qiang Zhang Jixiong Ju Feng Li Linyue Zhao Xu | 2015 | International Journal of Mining Science and Technology2015，25，6： | 3 |
| 4 | Quantification of energy related industrial eco-efficiency of China显示文摘Improving eco-efficiency is propitious for saving resources and reducing emissions,and has become a popular route to sustainable development.We define two energy-related eco-efficiencies:energy efficiency(ENE)and greenhouse gas(GHG)emission-related eco-efficiency(GEE)using energy consumption and the associated GHG emissions as the environmental impacts.Using statistical data,we analyze China’s energy consumption and GHG emissions by industrial subsystem and sector,and estimate the ENE and GEE values for China in 2007 as 4.871×10^(7)US·/PJ and 4.26×10^(8)US$/TgCO_(2)eq,respectively.Industry is the primary contributing subsystem of China’s economy,contributing 45.2%to the total economic production,using 79.6%of the energy consumed,and generating 91.4%of the total GHG emissions.We distinguish the individual contributions of the 39 industrial sectors to the national economy,overall energy consumption,and GHG emissions,and estimate their energyrelated eco-efficiencies.The results show that although ferrous metal production contributes only 3.5%to the national industrial economy,it consumes the most industrial energy(20%of total),contributes 16%to the total industrial global warming potential(GWP),and ranks third in GHG emissions.The power and heat sector ranks first in GHG emissions and contributes one-third of the total industrial GWP,although it only consumes about 8%of total industrial energy and,like ferrous metal production,contributes 3.5%to the national economy.The ENE of the ferrous metal and power and heat sectors are only 8 and 2.1×10^(7)US$/PJ,while the GEE for these two sectors are 9 and 4×10^(4)US$/GgCO_(2)eq,respectively;these are nearly the lowest ENE and GEE values among all 39 industry sectors.Finally,we discuss the possibility of ecoefficiency improvement through a comparison with other countries. | Jiansu MAO Yanchun DU Linyu XU Yong ZENG | 2011 | Frontiers of Environmental Science & Engineering2011，5，4： | 1 |
| 5 | Environmental performanceevaluation of Beijing’s energy use planning 显示文摘 | Wang Lei Xu Linyu Song Huimin | 2011 | Energy Policy2011，，39： | 1 |
| 6 | Modeling a policy making framework for urban sustainability: Incorporating system dynamics into the Ecological Footprint显示文摘 | Wei Jin Linyu Xu Zhifeng Yang | 2009 | Ecological Economics2009，，12： | 1 |
| 7 | Modeling a policy making framework for urban sustainability:Incorporating system dynamics into the Ecological Footprint显示文摘 | Wei Jin Linyu Xu Zhifeng Yang | | 0，，12： | 1 |
| 8 | The Study of a Method of Regional Environmental Risk Assessmem 显示文摘 | Xu Linyu Liu Guiyou | 2009 | Journal of Environmental Management2009，90，11： | 1 |
| 9 | The study of a method of regional environmental risk assessment显示文摘 | Linyu Xu Guiyou Liu | 2009 | Journal of Environmental Management2009，，11： | 1 |
| 10 | The study of a method of regional environmental risk assessment显示文摘 | Linyu Xu Guiyou Liu | 2009 | Journal of Environmental Management2009，，11： | 1 |
| 11 | Evaluation of urban ecological carrying capacity : a case study of Beijing, China 显示文摘 | Xu Linyu Kang Peng Wei Jinjin | 2010 | Procedia Environmental Sciences2010，2，6： | 1 |
| 12 | The study of a method of regional environmental risk assessment 显示文摘 | XU Linyu LIU Guiyou | 2009 | J Environ Manage2009，90，1： | 1 |
| 13 | Urban Ecosystem Health Assessment: Perspectives and Chinese Practice显示文摘 | Su Meirong Zhang Yan Liu Gengyuan Xu Linyu Zhang Lixiao Yang Zhifeng | 2013 | International Journal of Environmental Research and Public Health2013，，： | 1 |
| 14 | Approach for aggregate risk assessment of urban development zones 显示文摘 | XU Linyu SHU Xin | 2012 | Procedia Environmental Sciences2012，13，： | 1 |
| 15 | Eco-security Monitoring Index System for Urban Development Zone显示文摘 | Xiaocui Zhou Zhifeng Yang Linyu Xu | 2010 | Procedia Environmental Sciences2010，，： | 1 |
| 16 | CHIP controls the sensitivity of transforming growth factor-b signaling by modulating the basal level of Smad3 through ubiquitin-mediated degradation显示文摘 | Xin H xu X Linyu L et la | 2005 | J Biol Chem2005，280，20： | 1 |
| 17 | Changes of learning and memory ability associated with neuronal nitric oxide synthase in brain tissues of rats with acute alcoholism显示文摘BACKGROUD: Ethanol can influence neural development and the ability of learning and memory, but its mechanism of the neural toxicity is not clear till now. Endogenous nitric oxide (NO) as a gaseous messenger is proved to play an important role in the formation of synaptic plasticity, transference of neuronal information and the neural development, but excessive nitro oxide can result in neurotoxicity. OBJECTIVE: To observe the effects of acute alcoholism on the learning and memory ability and the content of neuronal nitric oxide synthase (nNOS) in brain tissue of rats. DESIGN: A randomized controlled animal experiment. SETTING: Department of Physiology, Xinxiang Medical College. MATERIALS: Eighteen male clean-degree SD rats of 18-22 weeks were raised adaptively for 2 days, and then randomly divided into control group (n = 8) and experimental group (n = 10). The nNOS immunohistochemical reagent was provided by Beijing Zhongshan Golden Bridge Biotechnology Co.,Ltd. Y-maze was produced by Suixi Zhenghua Apparatus Plant. METHODS: The experiment was carried out in the laboratory of the Department of Physiology, Xinxiang Medical College from June to October in 2005. ① Rats in the experimental group were intraperitoneally injected with ethanol (2.5 g/kg) which was dissolved in normal saline (20%). The loss of righting reflex and ataxia within 5 minutes indicated the successful model. Whereas rats in the control group were given saline of the same volume. ② Examinations of learning and memory ability: The Y-maze tests for learning and memory ability were performed at 6 hours after the models establishment. The rats were put into the Y-maze separately. The test was performed in a quiet and dark room. There was a lamp at the end of each of three pathways in Y-maze and the base of maze had electric net. All the lamps of the three pathways were turned on for 3 minutes and then turned off. One lamp was turned on randomly, and the other two delayed automatically. In 5 seconds after alternation, pulsating electric current presented in the base of unsafe area to stimulate rat’s feet to run to the safe area. The lighting lasted for 15 seconds as one test. Running from unsafe area to safe area at one time in 10 seconds was justified as successful. Such test was repeated for 10 times for each rat and the successful frequency was recorded. The qualified standard of maze test was that the rat arrived in the safe area 9 times during 10 experiments. The number of trainings for the qualified standard was used to represent the result of spatial learning. ③ Determination of the content of nNOS in brain tissue: After the Y-maze test, the rats were anaesthetized, and blood was let from the incision on right auricle, transcardially perfused via the left ventricle with about 200 mL saline, then fixed by perfusion of 40 g/L paraformaldehyde. Hippocampal CA1 region, corpus striatum and cerebellum were taken to prepare serial freezing coronal sections. The nNOS contents in the brain regions were determined with the immunohistochemical methods to reflect the changes of nitric oxide in brain tissue. MAIN OUTCOME MEASURES: The changes of learning and memory ability and the changes of the nNOS contents in the brain tissue of rats with acute alcoholism were observed. RESULTS: One rat in the experimental group was excluded due to its slow reaction to electric stimulation in the Y-maze test, and the other 17 rats were involved in the analysis of results. ① The training times to reach qualifying standards of Y-maze in the experimental group was more than that in the control group [(34.33 ±13.04), (27.50±8.79) times, P < 0.05]. ② Forms and numbers of nNOS positive neurons in brain tissue: It could be observed under light microscope that in the hippocampal CA1 region, there were fewer nNOS positive neurons, which were lightly stained, and the processes were not clear enough; But the numbers of the positive neurons which were deeply stained as buffy were obviously increased in the experimental group, the cell body and cytoplasm of process were evenly stained, but the nucleus was not stained. The nNOS positive neurons in corpus striatum had similar forms and size in the experimental group and control group. The form of the nNOS positive neurons in cerebellum were similar between the two groups. The numbers of nNOS positive neurons in hippocampal CA1 region and corpus striatum in the experimental group [(18.22±7.47), (11.38±5.00) cells/high power field] were obviously higher than those in the control group [(10.15±4.24), (6.15±3.69) cells/high power field. The number of nNOS positive neurons in cerebellum had no significant difference between the two groups [(49.56±18.84), (44.43±15.42) cells/high power field, P > 0.05]. CONCLUSION: Acute alcoholism may impair learning and memory ability, and nitric oxide may be involved in mediating the neurotoxic role of ethanol. | Shuang Li Chunyang Xu Dongliang Li Xinjuan Li Linyu Wei Yuan Cheng | 2006 | Neural Regeneration Research2006，1，3： | 1 |
| 18 | SARS-CoV-2 immunity and functional recovery of COVID-19 patients 1-year after infection显示文摘The long-term immunity and functional recovery after SARS-CoV-2 infection have implications in preventive measures and patient quality of life.Here we analyzed a prospective cohort of 121 recovered COVID-19 patients from Xiangyang,China at 1-year after diagnosis.Among them,chemiluminescence immunoassay-based screening showed 99%(95%CI,98–100%)seroprevalence 10–12 months after infection,comparing to 0.8%(95%CI,0.7–0.9%)in the general population.Total anti-receptor-binding domain(RBD)antibodies remained stable since discharge,while anti-RBD IgG and neutralization levels decreased over time.A predictive model estimates 17%(95%CI,11–24%)and 87%(95%CI,80–92%)participants were still 50%protected against detectable and severe re-infection of WT SARS-CoV-2,respectively,while neutralization levels against B.1.1.7 and B.1.351 variants were significantly reduced.All non-severe patients showed normal chest CT and 21%reported COVID-19-related symptoms.In contrast,53%severe patients had abnormal chest CT,decreased pulmonary function or cardiac involvement and 79%were still symptomatic.Our findings suggest long-lasting immune protection after SARS-CoV-2 infection,while also highlight the risk of immune evasive variants and long-term consequences for COVID-19 survivors. | Yan Zhan Yufang Zhu Shanshan Wang Shijun Jia Yunling Gao Yingying Lu Caili Zhou Ran Liang Dingwen Sun Xiaobo Wang Zhibing Hou Qiaoqiao Hu Peng Du Hao Yu Chang Liu Miao Cui Gangling Tong Zhihua Zheng Yunsheng Xu Linyu Zhu Jin Cheng Feng Wu Yulan Zheng Peijun Liu Peng Hong | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 0 |
| 19 | The theory of critical flicker fusion frequency and its application in cataracts显示文摘Background:Due to media opacity,it is usually difficult to accurately evaluate the postoperative visual acuity in cataracts patients.As a small and portable tool,the critical flicker fusion frequency(CFF)device reflects the temporal resolution of visual function and has been widely used in clinical research.However,poor understanding of the technique and equipment limitations have restricted its clinical application in China.Main text:There was a decrease in the CFF value in various ophthalmic diseases,indicating that the CFF is sensitive to detect visual functional changes.A number of studies have shown that the CFF test can accurately distinguish patients with simple cataracts from those with cataracts combined with fundus disease,and,as a visual test,it can more accurately predict postoperative visual acuity without being affected by media opacity.This study comprehensive reviews the basic principles of CFF and its application in ophthalmology,especially in cataracts.Conclusions:As one of the tools for dynamic visual function detection,the CFF test could help doctors to assess the possible presence of fundus disease in cataracts patients,especially in eyes with dense cataracts,and more precisely provide a reasonable visual prognosis than other available visual tests. | Guangcan Xu Junxia Fu Haolan Qi Linyu Li Wenqian Chen Yi Gao Tianju Ma Zi Ye Zhaohui Li | 2023 | Advances in Ophthalmology Practice and Research2023，3，1： | 0 |
| 20 | Network pharmacology-based analysis on three amicoumacin-type isocoumarin compounds from an endophytic bacterium in Houttuynia cordata显示文摘Amicoumacin isocoumarin-type compounds are mainly derived from the secondary metabolites of Bacillus bacteria,and have potential biological activities such as antibacterial,anti-tumor,anti-inflammatory,and anti-ulcer.In our previous research,three amicoumacin-type isocoumarin compounds,Paenicoumacin A,Paenicoumacin B,and Paenicoumacin C(B and C are novel),were isolated from an endophytic bacterium in Houttuynia cordata.In this study,network pharmacology and bioinformatics techniques were used to analyze the potential activities of amicoumacin compounds in the treatment of liver cancer.These compounds were found to mainly regulate cellular oxidative stress,cancer process,nucleic acid metabolism,nutritional metabolism and other pathways.The findings provide useful information for subsequent experimental verification. | Yiyao Lu Yinjie Xiong Mengyue Zhang Yingying Wu Jingjing Xu Linyu Zhang Xin Wu | 2021 | Asian Journal of Traditional Medicines2021，16，5： | 0 |
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