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| 1 | Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex显示文摘The nuclear pore complex(NPC)mediates the flow of substances between the nucleus and cytoplasm in eukaryotic cells.Here we report the cryo-electron tomography(cryo-ET)structure of the luminal ring(LR)of the NPC from Xenopus laevis oocyte.The observed key structural features of the LR are independently confirmed by single-particle cryo-electron microscopy(cryo-EM)analysis.The LR comprises eight butterfly-shaped subunits,each containing two symmetric wings.Each wing consists of four elongated,tubular protomers.Within the LR subunit,the eight protomers form a Finger domain,which directly contacts the fusion between the inner and outer nuclear membranes and a Grid domain,which serves as a rigid base for the Finger domain.Two neighboring LR subunits interact with each other through the lateral edges of their wings to constitute a Bumper domain,which displays two major conformations and appears to cushion neighboring NPCs.Our study reveals previously unknown features of the LR and potentially explains the elastic property of the NPC. | Yanqing Zhang Sai Li Chao Zeng Gaoxingyu Huang Xuechen Zhu Qifan Wang Kunpeng Wang Qiang Zhou Chuangye Yan Wusheng Zhang Guangwen Yang Minhao Liu Qinghua Tao Jianlin Lei Yigong Shi | 2020 | Cell Research2020，30，6： | 4 |
| 2 | Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis显示文摘The nuclear pore complex(NPC)exhibits structural plasticity and has only been characterized at local resolutions of up to 15 A for the cytoplasmic ring(CR).Here we present a single-particle cryo-electron microscopy(cryo-EM)structure of the CR from Xenopus laevis NPC at average resolutions of 5.5-7.9A,with local resolutions reaching 4.5 A.Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components.The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits,forming a circular scaffold.Within each CR subunit,the Nup358-containing region wraps around the stems of both Y complexes,likely stabilizing the scaffold.Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighboring Y complex.The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC.These previously uncharacterized structural features reveal insights into NPC assembly. | Gaoxingyu Huang Yanqing Zhang Xuechen Zhu Chao Zeng Qifan Wang Qiang Zhou Qinghua Tao Minhao Liu Jianlin Lei Chuangye Yan Yigong Shi | 2020 | Cell Research2020，30，6： | 2 |
| 3 | Cryo-EM structure of the inner ring from the Xenopus laevis nuclear pore complex显示文摘Nuclear pore complex(NPC)mediates nucleocytoplasmic shuttling.Here we present single-particle cryo-electron microscopy structure of the inner ring(IR)subunit from the Xenopus laevis NPC at an average resolution of 4.2Å.A homo-dimer of Nup205 resides at the center of the IR subunit,flanked by two molecules of Nup188.Four molecules of Nup93 each places an extended helix into the axial groove of Nup205 or Nup188,together constituting the central scaffold.The channel nucleoporin hetero-trimer of Nup62/58/54 is anchored on the central scaffold.Six Nup155 molecules interact with the central scaffold and together with the NDC1–ALADIN hetero-dimers anchor the IR subunit to the nuclear envelope and to outer rings.The scarce inter-subunit contacts may allow sufficient latitude in conformation and diameter of the IR.Our structure reveals the molecular basis for the IR subunit assembly of a vertebrate NPC. | Gaoxingyu Huang Xiechao Zhan Chao Zeng Ke Liang Xuechen Zhu Yanyu Zhao Pan Wang Qifan Wang Qiang Zhou Qinghua Tao Minhao Liu Jianlin Lei Chuangye Yan Yigong Shi | 2022 | Cell Research2022，32，5： | 1 |
| 4 | Regulation of the inward K^+-channels in stomatal guard cells by cytoskeletal microtubules显示文摘Patch clamp techniques were applied to investigating the regulation of the inward K^+channels in Vicia stomatal guard cells by cytoskeletal microtubules. The intracellular addition of either microtubule-disassembling reagent amprophos-methyl (APM) or microtubule-stabilizing reagent taxol resulted in significant inhibition of the inward K^+ -currents across the plasma membranes of Vicia stomatal guard cells. The results suggest that the activation of the inward K^+channels in stomatal guard cells requires proper dynamic organization of cytoskeletal microtubules. The regulation of the inward K^+-channels in guard cells by microtubules may mediate the regulation of stomatal movements by cytoskeleton. | Ximing Zhou Weihua Wu Ming Yuan Xuechen Wang | 1999 | Chinese Science Bulletin1999，44，10： | 1 |
| 5 | Design,synthesis,and evaluation of PD-L1 degraders to enhance T cell killing activity against melanoma显示文摘Inhibitor targeting immune checkpoint is a promising new anticancer therapy.Blocking the interaction between PD-1 and PD-L1 can reverse the immunosuppression state and improve the lethality of immune cells to tumor cells.Here,we report PROTAC-based PD-L1 degraders to enhance T cell killing activity against melanoma.Four series of PD-L1 degraders were designed and synthesized to VHL,CRBN,MDM2 or c IAP E3 ligase system,in which CRBN-ligand-based compound BMS-37-C3 was identified as the most active PROTAC molecule.BMS-37-C3 also significantly enhanced the killing ability of T cells in a co-culture model of A375 and T cells.Furthermore,western blot data and flow cytometry demonstrated that BMS-37-C3 could reduce the protein levels of PD-L1 in dose and time dependent manner,which may provide a new therapeutic method for tumor immunotherapy. | Yang Liu Mengzhu Zheng Zhilu Ma Yirong Zhou Junfeng Huo Wenbo Zhang Yu Liu Yuanyuan Guo Xuechen Zhou Hua Li Lixia Chen | 2023 | Chinese Chemical Letters2023，34，5： | 0 |
| 6 | Towards a tailored indoor horticulture:a functional genomics guided phenotypic approach显示文摘As indoor horticulture gathers momentum,electric(also termed artificial)lighting systems with the ability to generate specific and tunable wavelengths have been developed and applied.While the effects of light quality on plant growth and development have been studied,authoritative and reliable sets of light formulae tailored for the cultivation of economically important plants and plant traits are lacking as light qualities employed across laboratories are inconsistent.This is due,at least in part,to the lack of molecular data for plants examined under electric lights in indoor environments.It has hampered progress in the field of indoor horticulture,in particular,the transition from small-scale indoor farming to commercial plant factories.Here,we review the effects of light quality on model and crop plants studied from a physiological,physical and biochemical perspective,and explain how functional genomics can be employed in tandem to generate a wealth of molecular data specific for plants cultivated under indoor lighting.We also review the current state of lighting technologies in indoor horticulture specifically discussing how recent narrow-bandwidth lighting technologies can be tailored to cultivate economically valuable plant species and traits.Knowledge gained from a complementary phenotypic and functional genomics approach can be harvested not only for economical gains but also for sustainable food production.We believe that this review serves as a platform that guides future light-related plant research. | Claudius Marondedze Xinyun Liu Shihui Huang Cynthia Wong Xuan Zhou Xutong Pan Huiting An Nuo Xu Xuechen Tian Aloysius Wong | 2018 | Horticulture Research2018，5，1： | 0 |
| 7 | Strigolactones positively regulate abscisic acid-dependent heat and cold tolerance in tomato显示文摘Strigolactones are carotenoid-derived phytohormones that impact plant growth and development in diverse ways.However,the roles of strigolactones in the responses to temperature stresses are largely unknown.Here,we demonstrated that strigolactone biosynthesis is induced in tomato(Solanum lycopersicum)by heat and cold stresses.Compromised strigolactone biosynthesis or signaling negatively affected heat and cold tolerance,while application of the synthetic strigolactone analog GR245DS enhanced heat and cold tolerance.Strigolactone-mediated heat and cold tolerance was associated with the induction of abscisic acid(ABA),heat shock protein 70(HSP70)accumulation,C-REPEAT BINDING FACTOR 1(CBF1)transcription,and antioxidant enzyme activity.Importantly,a deficiency in ABA biosynthesis compromised the GR245DS effects on heat and cold stresses and abolished the GR245DS-induced transcription of HSP70,CBF1,and antioxidant-related genes.These results support that strigolactones positively regulate tomato heat and cold tolerance and that they do so at least partially by the induction of CBFs and HSPs and the antioxidant response in an ABA-dependent manner. | Cheng Chi Xuechen Xu Mengqi Wang Hui Zhang Pingping Fang Jie Zhou Xiaojian Xia Kai Shi Yanhong Zhou Jingquan Yu | 2021 | Horticulture Research2021，8，1： | 0 |
| 8 | Thermally stable poly(3-hexylthiophene):Nonfullerene solar cells with efficiency breaking 10%显示文摘Solar cells featuring polythiophenes as donors are one of the optoelectronic devices that hold notable promises for commercial application,profiting from the lowest synthetic complexity and excellent scalability.However,the complex phase behaviors of polythiophenes and their blends put constraints on modulating electrical performance and thus realizing stable performance under thermal stress.In this contribution,we present a multi-technique approach that combines calorimetry,scattering,spectroscopy,and microscopy to thoroughly probe the thermodynamic mixing,thermal properties of materials,the evolution of nanoscale domain structure,and device performance of poly(3-hexylthiophene)(P3HT)with a range of nonfullerene acceptors(NFAs)such as ITIC,IDTBR,and ZY-4Cl.Accordingly,two blending guidelines are established for matching these popular NFAs with P3HT to enable highly efficient and thermally stable cells.First,blend systems with weak vitrification and hypo-miscibility are excellent candidates for efficient solar cells.Furthermore,high thermal stability can be achieved by selecting NFAs with diffusion-limited crystallization.The P3HT:ZY-4Cl blend was found to endow the best performance of over 10%efficiency and an exceptionally high T_(80) lifetime of>6000 h under continuous thermal annealing,which are among the highest values for P3HT-based solar cells.This realization of high thermal stability and efficiency demonstrates the remarkable potentials of simple polythiophene:nonfullerene pairs in electronic applications. | Mengyuan Gao Yang Liu Kaihu Xian Zhongxiang Peng Kangkang Zhou Junwei Liu Saimeng Li Fei Xie Wenchao Zhao Jidong Zhang Xuechen Jiao Long Ye | 2022 | Aggregate2022，3，5： | 0 |
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