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| 1 | The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis显示文摘Eucommia ulmoides,也叫的强壮的橡胶树,是一棵经济地重要的树;然而,它的染色体顺序的缺乏 ?限制基本生物研究和这植物种的应用研究。这里,我们在场它的 1.2-Gb 染色体的一个高质量的集会(支架 N50 ?=? 1.88 Mb ) 与至少 26 ? 为 E 的 723 预言的基因。ulmoides,顺序 Garryales 的首先定序的染色体,它用联合定序的 Illumina 的综合策略被获得,定序的 PacBio,和印射的 BioNano。作为到 lamiids 和 campanulids 的姐妹 taxon, E。ulmoides 经历了三倍由核心 eudicots 而是没有进一步整个染色体的复制分享了的一个古老的染色体 ? 在最后 1.25 亿年里。E。ulmoides 为涉及压力回答和第二等的代谢物的生合成的多重基因展出高表达式层次或基因数字扩大,它可以说明它的可观的环境适应性。与橡胶树(Hevea brasiliensis ) 相对照,它生产 cis 聚异式戊二我烯, E。ulmoides 演变综合经由 farnesyl diphosphate synthases (FPS ) 的长链的 trans 聚异式戊二我烯。而且, FPS 和橡胶延伸 factor/small 橡胶粒子蛋白质基因家庭从 H 独立地被扩展。brasiliensis 系。这些结果提供新卓见进 E. 的生物学 ? ulmoides 和聚异式戊二我烯生合成的起源。 | Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du | 2018 | Molecular Plant2018，11，3： | 24 |
| 2 | SIMULATION AND EXPERIMENT OF BUBBLY FLOW INSIDE THROTTLING GROOVE显示文摘在在扼杀沟内的压力分发和成穴(噪音) 之间的关系被数字模拟和试验性的方法调查。有几个变换器的一个阀门衣袋被执行在阀门房间内检测压力分布,并且结果与计算液体动力学(CFD ) 适合相当好分析。高速度的成像技术被采用在扼杀的沟附近调查成穴机制,特别地水泡开始和簇形成。一个光谱分析器被用来测量水泡流动产生的噪音的健全压力水平。在沟内的压力分布对阀门港口配置和背压力敏感,这被发现。压力分发决定通过阀门沟和折叠水泡导致的噪音的健全压力水平的水泡尺寸和数字。入口压力主要因此在沟和健全压力水平的紧张内影响冒泡的流动的浸透度。 | FU Xin DU Xuewen ZOU Jun YANG Huayong JI Hong | 2007 | Chinese Journal of Mechanical Engineering2007，20，5： | 7 |
| 3 | Enzymatic formation of curcumin in vitro and in vivo显示文摘 | Jiaqing wang Taotao Xiong Jie Zhou Hongjian He Dongdong Wu Xuewen Du Xingyi Li Bing Xu | 2018 | Nano Research2018，11，6： | 1 |
| 4 | A simple and efficient method of DNA extraction from formalin-fixed and paraffin-embedded tissues for screening EGFR gene mutation by gene sequencing显示文摘Epidermal growth factor receptor (EGFR) is frequently overexpressed in non-small-cell lung cancer (NSCLC) and plays a key role in tumorigenesis.1 Small molecule tyrosine kinase inhibitors (TKIs),such as gefitinib,erlotinib,and icotinib,which can inhibit receptor tyrosine kinase activity of EGFR become clinically available for the treatment of non-small-cell lung cancer (NSCLC).2 NSCLC patients with EGFR mutation have experienced a marked response to EGFR-TKIs therapy.3 Detection of mutations of the EGFR gene is critical for predicting the response to therapy with TKIs. | Zhang Dan Cao Zhenqiang Zhang Guizhen Wu Mei Zhang Yucheng Du Zhenwu Zhang Xuewen | 2014 | Chinese Medical Journal2014，，11： | 1 |
| 5 | Experimental study on the vitrification and xenotransplantation of human ovarian tissue显示文摘Background:Cryopreservation of ovarian tissue is a promising method for preserving fertility.Transmission electron microscopy(TEM)is an evaluation system for cryo-injury during the cooling and warming process which is very laborious and needs to be optimized.Objective:In this study,we evaluated that serum 17β-oestradiol(E2)may be used as an indicator of vitrified ovarian tissue.Methods:Immunodeficient nude mice were used as hosts for xenografting of vitrified-warmed human ovarian tissues.A total of 54 mice were divided into two group:vitrified ovarian xenotransplant(VOX)group(n?45)and non-transplant control group(n?9).The transplanted mice were grouped into vitrified/warmed grafted-4 weeks(VOX-4w,n?15),vitrified/warmed grafted-6weeks(VOX-6w n?15)and vitrified/warmed grafted-12 weeks(VOX-12w n?15)according to the time after transplantation.The viable and functional recovery of grafted ovarian tissue was assessed by light microscopy,transmission electron microscopy,and hormone(E2)assays.Results:Serum E2 concentration was significantly higher in VOX-6w(group(21.07 pg/ml)than that of VOX-12w group(15.59 pg/ml).VOX-12w group showed a lower value(12.61 pg/ml)for E2 concentration.The trend for E2 concentration was consistent with the morphological identification of the grafts.Conclusion:In vivo serum hormone E2 released by cortical biopsies can be used as a functional marker for xenotransplanted vitrified-warmed human ovarian tissue reserve. | Jinghui Zhao Yuping Ge Jingyi Li Yucui Zeng Guoyi Dong Liping Zeng Juanjuan Dai Hui Du Mumin Shao Xuewen Yu Huan Li Fang He Zhouchun Shang Chun Wang Gabor Vajt Huiru Tang Ruifang Wu Yutao Du | 2022 | Gynecology and Obstetrics Clinical Medicine2022，2，1： | 1 |
| 6 | Porcine TEF1 and RTEF1 : Molecular characterization and association analyses with growth traits显示文摘 | Xuewen Xu Shuang Xing Zhi-Qiang Du Max. F. Rothschild Martine Yerle Bang Liu | 2008 | Comparative Biochemistry and Physiology Part B2008，，4： | 1 |
| 7 | Effects of Mangiferin on the Expression of TNF-α,iNos,ICAM-1 and Its mRNA in the Heart,Brain and Kidneys of SHR显示文摘[Objectives] To study the effects of Mangiferin( MGF) on TNF-α,iNOS,ICAM-1 and its mRNA expression in the heart,brain and kidneys of spontaneously hypertensive rats( SHR),and reveal the mechanism of its anti-inflammatory injury in hypertension target organs.[Methods]SHRs were randomly divided into 5 groups: the model group,the high-dose,medium-dose,low-dose MGF groups and the Benazepril group,with 8 rats in each group,WKY rats were used for the normal control group. Besides,on-invasive blood pressure( BP) instruments were used to measure systolic blood pressure in the rats' tail artery,western blot was used to analyze the expression of TNF-α,iNOS,ICAM-1 and reverse transcription-polymerase chain reaction( RT-PCR) was used to analyze the expression of TNF-α,iNOS,ICAM-1 mRNA.[Results]Compared with the normal control group,the model group's BP level was significantly increased( P <0. 01)),but the MGF had no significant lowering BP effect( P > 0. 05); compared with the normal control group,the expression of TNF-α,iNOS,ICAM-1 and its' mRNA in the model group was significantly increased( P < 0. 05 or P < 0. 01),and MGF could reduce the level of expression of these inflammatory cytokines( P < 0. 05 or P < 0. 01); between the Benazepril group and high-dose,medium-dose,low-dose MGF groups,most of the indicators had no significant difference( P > 0. 05). [Conclusions]MGF had no significant lowering BP effect,SHR showed inflammatory injury in the heart,brain and kidneys,MGF showed improvement on the inflammatory injury,and the anti-inflammation mechanism may be associated with lowering TNF-α,i NOS and ICAM-1 and its mRNA expression. | Xiaoqin HU Xuewen ZENG Erwei HAO Zhengcai DU Bei ZHOU Jiagang DENG | 2018 | Medicinal Plant2018，9，2： | 0 |
| 8 | Mangiferin alleviates renal infammatory injury in spontaneously hypertensive rats by inhibiting MCP-1/CCR2 signaling pathway显示文摘Objective: Hypertension is a low-grade infammation state of the disease and was easily complicated by kidneys’ infammatory response. Mangiferin(MGF), a pharmacologically active compound in various plants including Mangifera indica, has a strong anti-infammatory activity. However, the effects of MGF on renal infammatory injury in spontaneously hypertensive rats(SHRs) remain unclear. The purpose of this study was to investigate the protective effects and mechanisms of MGF on renal infammatory injury in SHRs.Methods: MGF was used in SHRs at the doses of 10, 20, 40 mg/kg/d for 8 weeks consecutively. The blood and urine were collected for assessment of renal function. Renal tissues were collected for histological,immunohistochemistry, ELISA, Western blot and real time reverse transcription PCR(RT-PCR) analysis.Results: The results showed that the levels of interleukin 6(IL-6), tumor necrosis factor-a(TNF-a), monocyte chemoattractant protein-1(MCP-1) and recombinant chemokine C-C-Motif receptor 2(CCR2) were increased in SHRs, meanwhile, the level of IL-10 was decreased in SHR. Treatment of MGF inhibited the expression of IL-6, TNF-a, MCP-1 and CCR2, and promoted the expression of IL-10. Furthermore, the content of blood urea nitrogen(BUN) and serum uric acid(SUA) was significantly increased in the model group, and treatment of MGF had no obvious effects on these parameters at all dose levels.Conclusion: Our study proved that the kidneys of SHRs had significant infammatory injury, and MGF had the protective effects on renal infammatory injury in SHRs;The protective mechanism may be mediated partly by the MCP-1/CCR2 signaling pathway. Thus, it is a potential new drug for the treatment of hypertension. | Xiaoqin Hu Wei Zhao Jiagang Deng Zhengcai Du Xuewen Zeng Bei Zhou Erwei Hao | 2023 | Chinese Herbal Medicines2023，15，4： | 0 |
| 9 | Long non-coding RNA-AK138945 regulates myocardial ischemia-reperfusion injury via the miR-1-GRP94 signaling pathway显示文摘Objective:Myocardial ischemia-reperfusion injury(MIRI)is one of the leading causes of death from cardiovascular disease in humans,especially in individuals exposed to cold environments.Long non-coding RNAs(lncRNAs)regulate MIRI through multiple mechanisms.This study explored the regulatory effect of lncRNA-AK138945 on myocardial ischemia-reperfusion injury and its mechanism.Methods:In vivo,8-to 12-weeks-old C57BL/6 male mice underwent ligation of the left anterior descending coronary artery for 50 minutes followed by reperfusion for 48 hours.In vitro,the primary cultured neonatal mouse ventricular cardiomyocytes(NMVCs)were treated with 100μmol/L hydrogen peroxide(H_(2)O_(2)).The knockdown of lncRNA-AK138945 was evaluated to detect cardiomyocyte apoptosis,and a glucose-regulated,endoplasmic reticulum stress-related protein 94(GRP94)inhibitor was used to detect myocardial injury.Results:We found that the expression level of lncRNA-AK138945 was reduced in MIRI mouse heart tissue and H2O2-treated cardiomyocytes.Moreover,the proportion of apoptosis in cardiomyocytes increased after lncRNA-AK138945 was silenced.The expression level of Bcl2 protein was decreased,and the expression level of Bad,Caspase 9 and Caspase 3 protein was increased.Our further study found that miR-1a-3p is a direct target of lncRNA-AK138945,after lncRNA-AK138945 was silenced in cardiomyocytes,the expression level of miR-1a-3p was increased while the expression level of its downstream protein GRP94 was decreased.Interestingly,treatment with a GRP94 inhibitor(PU-WS13)intensified H2O2-induced cardiomyocyte apoptosis.After overexpression of FOXO3,the expression levels of lncRNA-AK138945 and GRP94 were increased,while the expression levels of miR-1a-3p were decreased.Conclusion:LncRNA-AK138945 inhibits GRP94 expression by regulating miR-1a-3p,leading to cardiomyocyte apoptosis.The transcription factor Forkhead Box Protein O3(FOXO3)participates in cardiomyocyte apoptosis induced by endoplasmic reticulum stress through up-regulation of lncRNA-AK138945. | Yanying Wang Jian Huang Han Sun Jie Liu Yingchun Shao Manyu Gong Xuewen Yang Dongping Liu Zhuo Wang Haodong Li Yanwei Zhang Xiyang Zhang Zhiyuan Du Xiaoping Leng Lei Jiao Ying Zhang | 2024 | Frigid Zone Medicine2024，4，1： | 0 |
| 10 | Structure-Enhanced Mechanically Robust Graphite Foam with Ultrahigh MnO_(2) Loading for Supercapacitors显示文摘With the fast bloom of flexible electronics and green vehicles,it is vitally important to rationally design and facilely construct customized functional materials with excellent mechanical properties as well as high electrochemical performance.Herein,by utilizing two modern industrial techniques,digital light processing(DLP)and chemical vapor deposition(CVD),a unique 3D hollow graphite foam(HGF)is demonstrated,which shows a periodic porous structure and robust mechanical properties.Finite element analysis(FEA)results confirm that the properly designed gyroidal porous structure provides a uniform stress area and mitigates potential structural failure caused by stress concentrations.A typical HGF can show a high Young’s modulus of 3.18 MPa at a low density of 48.2 mg cm^(-3).The porous HGF is further covered by active MnO_(2) material with a high mass loading of 28.2 mg cm^(-2)(141 mg cm^(-3)),and the MnO_(2)/HGF electrode still achieves a satisfactory specific capacitance of 260 F g^(-1),corresponding to a high areal capacitance of 7.35 F cm^(-2) and a high volumetric capacitance of 36.75 F cm^(-3).Furthermore,the assembled quasi-solid-state asymmetric supercapacitor also shows remarkable mechanical properties as well as electrochemical performance. | Qinghe Cao Junjie Du Xiaowan Tang Xi Xu Longsheng Huang Dongming Cai Xu Long Xuewen Wang Jun Ding Cao Guan Wei Huang | 2020 | Research2020，，1： | 0 |
| 11 | Supramolecular Nanofibers/Hydrogels of the Conjugates of Nucleobase,Saccharide,and Amino Acids显示文摘Three new hydrogelators based on the conjugates of three naturally occurring biological building blocks:nu-cleobase,saccharide,and amino acids,were explored.Being synthesized via a facile solid phase peptide synthesis route,the hydrogelators self-assemble in water to afford supramolecular nanofibers and hydrogels.Transmission electron microscopy,oscillation rheometry,and circular dichroism reveal that the hydrogels consist of largely he-lix-based nanofibers of the widths of 5-12 nm and exhibit storage moduli up to 1 kPa.These hydrogelators also exhibit excellent cell-compatibility.This work illustrates a new platform for constructing molecular soft materials for nanobiotechnology. | Dongdong Wu Xuewen Du Junfeng Shi Jie Zhou Bing Xu | 2014 | Chinese Journal of Chemistry2014，32，4： | 0 |
| 12 | Screening and identification of candidate Fusarium wilt-resistance genes from pumpkin显示文摘Pumpkin(Cucurbita moschata)has been widely used as cucumber(Cucumis sativus L.)rootstock to defend against Fusarium wilt(FW)and increase cucumber yields and profits.However,the resistance genes and mechanisms underlying the FW tolerance in pumpkin are poorly understood.Here we analyzed the transcriptome of pumpkin inoculated with the cucumber FW causal agent Fusarium oxysporum f.sp.cucumerinum(Foc),and obtained 3152 and 4735 upregulated genes induced by Foc at 24h after Foc inoculation compared with at 0h and 24h non-inoculated control,respectively.Next,404 common differentially expressed genes(DEGs)were screened using the criterion log_(2) FPKM(fold change)≥2.In total,206 of 404 DEGs were predominantly expressed in roots,which is the first tissue that Foc contacts and invades.140 DEGs were selected and classified into four groups(pathogenesis resistance,secondary metabolism-related,transcription factor and signal binding)based on their functional descriptions.Then,29 genes having high expression levels were selected to investigate the expression patterns induced by a Foc inoculation.Among them,16 genes were significantly induced by Foc and showed high expression levels at various treatment time points.These candidate genes may act as positive regulators of FW resistance in pumpkin and provide effective resources for improving cucumber FW resistance through breeding programs. | Jun Xu Qianqian Xian Ke Wang Jingping Dong Chaoyang Zhang Shengli Du Xuewen Xu Xuehao Chen | 2022 | Horticultural Plant Journal2022，8，5： | 0 |
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