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| 1 | A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure显示文摘因为他们的人的白血球抗原(HLA ) haplotype 将强烈增加与房间线的相对更小的队为某些人口匹配的度,同型结合的人的胚胎的干细胞(hESCs ) 被认为是为 hESC 银行的更好的房间来源。同型结合的 hESCs 能从单性生殖的胚胎被产生,但是仅仅异质接合的 hESCs 用当前的策略被建立了没有第二极的身体挤出,人工地激活卵母细胞。这里,我们在 vitro 授精处理从 one-pronuclear 卵母细胞追随者例程报导一根人的同型结合的转换字符线(chHES-32 ) 的第一成功的推导。chHES-32 房间与正常 hESCs 表示普通标记和基因。他们处于一个无差别的状态被宣传超过一年(>P50 ) 了并且维持了 46 的稳定的 karyotype, XX。当在 vivo 并且在 vitro 区分了时, chHES-32 房间能从所有三胚胎的细菌层形成衍生物。五父亲一般地表示的印的基因和他们与卵母细胞施主相同的 HLA 遗传型的几乎无法发现的表示显示了他们的单性生殖的起源。用染色体宽的单个核苷酸的多型性分析并且 DNA 采指纹, chHES-32 房间的 homozygosity 进一步被证实。结果显示“讨厌”的 one-pronuclear 卵母细胞可能是为人的同型结合、单性生殖的转换字符的潜在的来源,并且为从单性生殖的 haploid 卵母细胞获得同型结合的 hESC 线建议了其他的策略。 | Ge Lin | 2007 | Cell Research2007，17，12： | 15 |
| 2 | Production of cloned transgenic cow expressing omega-3 fatty acids显示文摘 | Xia Wu Hongsheng Ouyang Biao Duan Daxin Pang Li Zhang Ting Yuan Lian Xue Daibang Ni Lei Cheng Shuhua Dong Zhuying Wei Lin Li Ming Yu Qing-Yuan Sun Da-Yuan Chen Liangxue Lai Yifan Dai Guang-Peng Li | 2012 | Transgenic Research2012，，3： | 1 |
| 3 | Near-infrared fluorophores with absolute aggregation-caused quenching and negligible fluorescence re-illumination for in vivo bioimaging of nanocarriers显示文摘Environment-responsive fluorophores with aggregation-caused quenching(ACQ)properties have been applied to track nanocarriers with reduced artefacts caused by unbound or free fluorophores but suffer from incomplete fluorescence quenching and significant re-illumination,which undermine bioimaging accuracy.Herein,through structural modifications to reinforce the hydrophobicity,planarity and rigidity of fluorophores with an aza-BODIPY framework,probes featuring absolute ACQ(aACQ)and negligible re-illumination are developed and evaluated in various nanocarriers.aACQ probes,FD-B21 and FD-C7,exhibit near-infrared emission,high quantum yield,photostability,water sensitivity,and negligible re-illumination in blood,plasma and 1%Tween-80 in contrast to ACQ probe P2 and conventional probe DiR.All nanocarriers can be labeled efficiently by the tested fluorophores.Polymeric micelles(PMs)labeled by different aACQ probes manifest similar biodistribution patterns,which however differ from that of DiR-labeled PMs and could be ascribed to the appreciable re-illumination of DiR.Significantly lower re-illumination is also found in aACQ probes(2%-3%)than DiR(20%-40%)in Caco-2,Hela,and Raw264.7 cells.Molecular dynamics simulations unravel the molecular mechanisms behind aggregation and re-illumination,supporting the hypothesis of planarity dependency.It is concluded that aACQ fluorophores demonstrate excellent water sensitivity and negligible fluorescence re-illumination,making themselves useful tools for more accurate bioimaging of nanocarriers. | Yifan Cai Xin Ji Yunsen Zhang Chang Liu Zichen Zhang Yongjiu Lv Xiaochun Dong Haisheng He Jianping Qi Yi Lu Defang Ouyang Weili Zhao Wei Wu | 2023 | Aggregate2023，4，2： | 0 |
| 4 | Holdout Problem and Its Governance in Market-Oriented Urban Renewal: Taking Shenzhen as an Example显示文摘This paper studies the occurrence, characteristics, and governance mechanism of the holdout problem during market-oriented urban renewal in Shenzhen after a related foreign experience analysis, through methodologies of logical analysis and case study.The results indicate that the holdout problem is almost an inevitable result of market-oriented urban renewal, and its proliferation not only goes against social morality and the principle of justice, but also harms social public interests, and even causes market failure.The paper proposes two approaches to dealing with holdout problem: first, to introduce the contracting commitment to restrict the owners’ freedom of contract, and to guarantee the public benefit of all owners;second, to introduce land expropriation right to regulate holdout behavior via authority of state. | Ouyang Yifan Du Jingshen Jin Xiangmu Liu Jinxin(译) | 2019 | China City Planning Review2019，28，2： | 0 |
| 5 | Reflection-type surface lattice resonances in all-metal metasurfaces for refractive index sensing显示文摘Surface lattice resonance(SLR)is a pretty effective mechanism to realize ultranarrow linewidths in the spectrum.Herein,we propose and demonstrate reflection-type SLRs in all-metal metasurfaces experimentally,compared with the traditional transmission-type SLR,which can avoid the refractive index(RI)mismatch problem and are more suitable for high-efficiency RI sensing due to direct contact and strong light–matter interaction.The measured SLR linewidth is 13.5 nm influenced by the meta-atom size,which needs a compromise design to keep a balance between the narrow linewidth and noise immunity.Notably,the SLR sensitivity is determined by the lattice period along the polarization direction with regularity,which establishes an intuitive link between structures and optical responses and provides a theoretical guide for metasurface designs.Additionally,incident angle multiplexing will make the resonance wavelength red shift or blue shift in the case of orthogonal polarization.The rectangular array metasurface can realize dual SLRs with different sensing performances.Flexibly,the SLR can also be formed by the different meta-atoms and arrays.This research supports SLR multifarious applications involving not only RI sensing but also nonlinear optics,nano-lasers,etc. | LIYE LI YIFAN OUYANG LIJUN MA HONGSHUN SUN YUSA CHEN MEIZHANG WU ZHIMEI QI WENGANG WU | 2023 | Photonics Research2023，11，12： | 0 |
| 6 | Acetylcholinesterase is associated with apoptosis in β cells and contributes to insulin-dependent diabetes mellitus pathogenesis显示文摘Acetylcholinesterase (疼痛) 表示在 apoptosis 期间是枢轴的。确实,疼痛禁止者部分保护房间免受 apoptosis 的伤害。胰岛素依赖者糖尿病 mellitus (IDDM ) 被胰腺的房间 apoptosis 部分地描绘。这里,我们在 IDDM 的发展调查了疼痛的角色并且分析了疼痛禁止者的保护的效果。多重低剂量的 streptozotocin (MLD-STZ ) 管理在一个老鼠模型导致了 IDDM。西方的污点分析,细胞化学的染色,并且染色的 immunofluorescence 被用来在 MIN6 房间,主要房间,和 apoptotic 检测疼痛表示 MLD-STZ-treated 老鼠的胰腺的房间。疼痛禁止者予 MLD-STZ 老鼠 intraperitoneally 被以 30 天。血葡萄糖,血浆胰岛素,和肌酸层次被测量,并且葡萄糖忍耐测试被执行。MIN6 房间上的疼痛禁止者的效果也被评估。当与 STZ 对待时,疼痛表示在 vivo 在 vitro 和胰腺的小岛在 apoptotic MIN6 房间和主要房间被导致。正式就职和在胰腺的小岛的疼痛的进步累积在 IDDM 开发期间与 apoptotic 房间被联系。疼痛禁止者的管理有效地减少了在 MLD-STZ 老鼠的糖尿病的多糖症和发生,和恢复血浆胰岛素层次和血浆肌酸清理。疼痛禁止者部分保护了 MIN6 房间免受 STZ 治疗引起的损坏的伤害。在胰腺的小岛和 IDDM 的发作和发展上的疼痛禁止者的保护的效果的疼痛的正式就职和累积显示在疼痛和 IDDM 之间的一种靠近的关系。 | Bao Zhang Lei Yang Luyang Yu Bo Lin Yanan Hou Jun Wu Qin Huang Yifan Han Lihe Guo Qi Ouyang Bo Zhang Lu Lu Xuejun Zhang | 2012 | Acta Biochimica et Biophysica Sinica2012，44，3： | 0 |
| 7 | Nanoparticles targeting at methylases with high correlation to N^(6)-methyladenosine-related lncRNA signatures as potential therapy of kidney clear cell carcinoma显示文摘Clear cell renal cell carcinoma(ccRCC)is a heterogeneous malignancy with poor prognosis.Methylation of the N^(6) position of adenosine(m^(6)A),the most common epigenetic modification in both messenger RNAs and noncoding RNAs,has been reported to regulate the initiation and progression of ccRCC.However,whether and how m^(6)A-related long noncoding RNAs(m^(6)ArlncRNAs)signify the progression of ccRCC remain unclear.We found m^(6)ArlncRNAs are effective signatures illustrating immune landscape and risk stratification in ccRCC.We identified two differently expressed m^(6)ArlncRNAs(DEm^(6)ArlncRNAs),AC008870.2 and EMX2OS,as independent risk factors for overall survival of ccRCC patients,by applying stringent variable selection procedure to data from the Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma project.The risk score generated from the DEm^(6)ArlncRNA expression categorizes patients into either high or low-risk groups,between which,enrichment analysis indicated an enrichment in immune-related pathways.Under different DEm^(6)ArlncRNA transcription pattern,the two risk groups differ in immune cell population composition and expression levels of therapy targeting genes.Nanoparticle is satisfactory strategy to delivering therapeutic drugs.For further clinical translation,we designed a novel nanoparticle delivery system packaged STM2457(STM@8P4 NPs),which selectively inhibits AC008870.2-correlated m^(6)A writer.STM@8P4 NPs loaded drug successfully with uniform particle size,long-term stability and high release efficiency.STM@8P4 NPs can easily enter ccRCC cells and showed a highly efficient ccRCC killing activity in vitro.Our results therefore indicate that m^(6)ArlncRNAs expression can depict tumor microenvironment,predict prognosis for ccRCC patient and give hint to therapeutic strategies in ccRCC. | Ruixuan Chen Ping Ouyang Licong Su Xi Xu Penghu Lian Yanqin Li Qi Gao Yifan Zhang Sheng Nie Fan Luo Ruqi Xu Xiaodong Zhang Xiaoxi Li Yue Cao Peiyan Gao Juanjuan Kang Jun Wu Lu Li | 2022 | Chinese Chemical Letters2022，33，10： | 0 |
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