3篇
您的检索式：作者名="YIN Wanxin"
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| 1 | IMPA2 blocks cervical cancer cell apoptosis and induces paclitaxel resistance through p53-mediated AIFM2 regulation显示文摘Cervical cancer continues to be a concern,and the prognosis of locally advanced cervical cancer remains poor.IMPA2 was previously identified as a potential oncogene and regulator of tumor apoptosis.In this study,we aim to further elucidate the underlying mechanisms of IMPA2 gene in the regulation of cervical cancer apoptosis.First,we identify AIFM2 as an upregulated gene in IMPA2-silenced cervical cancer cells,and inhibition of AIFM2 reverses IMPA2 knockdown-induced apoptosis.Further study reveals that AIFM2 regulates cell apoptosis in a mitochondrial-dependent manner with a redistribution of mitochondrial membrane potential and intracellular Ca^(2+) levels.However,the analysis of the STRING database and our experimental results show that AIFM2 has little effect on cervical cancer progression and survival.Further mechanistic study demonstrates that IMPA2 and AIFM2 silencing inhibits apoptosis by activating p53.Meanwhile,the knockdown of IMPA2 enhances the chemosensitivity of cervical cancer cells by strengthening paclitaxel-induced apoptosis.Based on the above results,the IMPA2/AIFM2/p53 pathway may be a new molecular mechanism for paclitaxel treatment of cervical cancer and an effective strategy to enhance the sensitivity of cervical cancer cells to paclitaxel.Our findings display a novel function of IMPA2 in regulating cell apoptosis and paclitaxel resistance mediated by a disturbance of AIFM2 and p53 expression,potentially making it a novel therapeutic target for cervical cancer treatment. | Kexin Xie Lei Liu Min Wang Xianping Li Bingqi Wang Sheng Yin Wanxin Chen Yingrui Lin Xiaolin Zhu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，4： | 0 |
| 2 | Mechanisms of imperatorin on regulating Pglycoprotein in blood-brain barrier based on network pharmacology and in vitro experiment显示文摘OBJECTIVE To explore mecha⁃nisms of imperatorin on regulating P-glycoprotein(P-gp)in blood-brain barrier(BBB)based on net⁃work pharmacology combined with in vitro experi⁃ment.METHODS Drug targets were predicted using the Pharmapper and Swiss targets data⁃bases;disease targets were obtained through the Genecards database;intersections between drugs and disease targets were screened by Cytoscape software;the obtained core targets were used to construct protein-protein interaction(PPI)network,gene ontology(GO)functions,and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis.The effects of imperatorin(20,50,100μmol·L^(-1))on P-gp activity were monitored in hCMEC/D3 in vitro BBB model,and the effects of imperatorin on the expression of target proteins were verified using Western blot method.RESULTS 55 drug targets and 3102 disease targets were obtained from the network pharmacology screening,and 37 core targets were obtained after the combination.Enrichment analysis showed that core targets were closely related to chemical synaptic trans⁃mission regulation,neurotransmitter receptor activity,protein kinase regulation activity,G proteincoupled receptor signaling pathway,neural active ligand receptor interaction pathway,PI3K-Akt sig⁃naling pathway,VEGF signaling pathway,etc..In vitro experimental validation suggested that all tested concentration groups of imperatorin signifi⁃cantly reduced the activity and expression of P-gp,which were achieved by significantly downregu⁃lating the phosphorylation levels of PI3K and Akt,and repressing the expression of VEGFR2 pro⁃tein.CONCLUSION Network pharmacology was used to predict the core targets and signaling pathways of imperatorin on regulating P-gp in BBB and relevant validation was conducted through in vitro experiments,providing a refer⁃ence basis for further exploration of the mecha⁃nisms of imperatorin on regulating P-gp in BBB. | SONG Yizhen YIN Wanxin MA Yicong AN Yufan HUANG Jiaqi YAN Zhongjie WU Xiuwen WANG Yuanyu | 2023 | 中国药理学与毒理学杂志2023，37，S01： | 0 |
| 3 | Mass Yield Distribution in Intermediate Energy Heavy Ion Reactions | Zhang, Xiang Li, Wenxin Yin, Xinmin Wen, Wanxin Sun, Tongyu Jin, Genming Luo, Qingzheng | 1995 | Chinese journal of nuclear physics1995，，3： | 0 |
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