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| 1 | Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation显示文摘在房间期间,分割要求忠诚有丝分裂的锭子汇编和染色体分离的基因信息的稳定的传播。运用了 GTPase 戏在有丝分裂的锭子汇编的一个关键角色。然而,在锭子的 Ran-GTP 的一个化学坡度的产生怎么被联合到有丝分裂的 translational 以后修正,从来没被描绘过。这里,我们解决了复杂结构与核苷酸版本因素 Mog1 跑了并且描出一个新奇有丝分裂特定的调整 acetylation 的 Ran-Mog1 相互作用在染色体分离期间。我们的指导结构的功能的分析表明那 Mog1 与 RCC1 竞争为以一种 GTP/GDP-dependent 方式变有约束力。生物化学的描述表明了那 Mog1 固定跑阻止 RCC1 有约束力、随后的 GTP 装载。令人惊讶地,跑了是 TIP60 的真正的底层,并且由 TIP60 的 Lys134 的 acetylation 解放 Mog1 从在有丝分裂期间变有约束力。重要地,这个得到 acetylation 的开关对 RCC1 变有约束力支持 Ran-GTP 的高水平,它为染色体排列是必要的。这些结果建立一以前 TIP60 由调节在提供 Ran-GTP 水平的 homeostatic 控制的规章的机制运用了的 uncharacterized 为在有丝分裂的染色体分离的受动器绑定。 | Xiaoling Bao Heng Liu Xing Liu Ke Ruan Yonshui Zhang Zhiyong Zhang Qi Hu Ying Liu Saima Akram Jiahai Zhang Qingguo Gong Wenwen wang Xiao Yuan Jian-Li Lingli Zhao Zhen Dou Ruijun Tian Xuebiao Yao Jihui Wu Yunyu Shi | 2018 | Journal of Molecular Cell Biology2018，10，1： | 8 |
| 2 | Control of flowering and inflorescence architecture in tomato bysynergistic interactions between ALOG transcription factors显示文摘lnflorescences are flower-bearing shoots that originate frompools of stem cells in shoot apical meristems (SAM). Inflorescencearchitecture is determined by a process of meristem maturation,during which stem cell fate switches from a vegetative to a repro-ductive growth program. | Xiaozhen Huang Lingli Tang Yuan Yu Justin Dalrymple Zachary B.Lippman Cao Xu | 2018 | Journal of Genetics and Genomics2018，45，10： | 4 |
| 3 | Targeting lactate dehydrogenase A (LDHA) exerts antileukemic effects on T-cell acute lymphoblastic leukemia显示文摘Background:T-cell acute lymphoblastic leukemia(T-ALL)is an uncommon and aggressive subtype of acute lymphoblastic leukemia(ALL).In the serum of T-ALL patients,the activity of lactate dehydrogenase A(LDHA)is increased.We proposed that targeting LDHA may be a potential strategy to improve T-ALL outcomes.The current study was conducted to investigate the antileukemic effect of LDHA gene-targeting treatment on T-ALL and the underlying molecular mechanism.Methods:Primary T-ALL cell lines Jurkat and DU528 were treated with the LDH inhibitor oxamate.MTT,colony formation,apoptosis,and cell cycle assays were performed to investigate the effects of oxamate on T-ALL cells.Quantitative real-time PCR(qPCR)and Western blotting analyses were applied to determine the related signaling pathways.A mitochondrial reactive oxygen species(ROS)assay was performed to evaluate ROS production after T-ALL cells were treated with oxamate.A T-ALL transgenic zebrafish model with LDHA gene knockdown was established using CRISPR/Cas9 gene-editing technology,and then TUNEL,Western blotting,and T-ALL tumor progression analyses were conducted to investigate the effects of LDHA gene knockdown on T-ALL transgenic zebrafish.Results:Oxamate significantly inhibited proliferation and induced apoptosis of Jurkat and DU528 cells.It also arrested Jurkat and DU528 cells in G0/G1 phase and stimulated ROS production(all P<0.001).Blocking LDHA significantly decreased the gene and protein expression of c-Myc,as well as the levels of phosphorylated serine/threonine kinase(AKT)and glycogen synthase kinase 3 beta(GSK-3β)in the phosphatidylinositol 3′-kinase(PI3K)signaling pathway.LDHA gene knockdown delayed disease progression and down-regulated c-Myc mRNA and protein expression in T-ALL transgenic zebrafish.Conclusion:Targeting LDHA exerted an antileukemic effect on T-ALL,representing a potential strategy for T-ALL treatment. | Haizhi Yu Yafei Yin Yifang Yi Zhao Cheng Wenyong Kuang Ruijuan Li Haiying Zhong Yajuan Cui Lingli Yuan Fanjie Gong Zhihua Wang Heng Li Hongling Peng Guangsen Zhang | 2020 | Cancer Communications2020，40，10： | 2 |
| 4 | Co-regulation of circadian clock genes and microRNAs in bone metabolism显示文摘Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It is widely recognized that circadian clock genes can regulate bone metabolism.In recent years,the regulation of bone metabolism by non-coding RNAs has become a hotspot of research.MicroRNAs can participate in bone catabolism and anabolism by targeting key factors related to bone metabolism,including circadian clock genes.However,research in this field has been conducted only in recent years and the mechanisms involved are not yet well established.Recent studies have focused on how to target circadian clock genes to treat some diseases,such as autoimmune diseases,but few have focused on the co-regulation of circadian clock genes and microRNAs in bone metabolic diseases.Therefore,in this paper we review the progress of research on the co-regulation of bone metabolism by circadian clock genes and microRNAs,aiming to provide new ideas for the prevention and treatment of bone metabolic diseases such as osteoporosis. | Tingting LI Shihua ZHANG Yuxuan YANG Lingli ZHANG Yu YUAN Jun ZOU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，7： | 2 |
| 5 | Development of a direct ELISA based on carboxy-terminal of penicillin-binding protein BlaR for the detection of β-lactam antibiotics in foods显示文摘 | Juan Peng Guyue Cheng Lingli Huang Yulian Wang Haihong Hao Dapeng Peng Zhenli Liu Zonghui Yuan | 2013 | Analytical and Bioanalytical Chemistry2013，，27： | 1 |
| 6 | Brominelpara-Toluenesulfonic Acid-Catalyzed Synthesis of 3,3-Bis(indole-3-yl)indoline-2-(1H)-ones by Condensing Indoles with Isatins显示文摘在仅仅在房间温度的 Br 2 的 3 mol% 的催化作用下面,吲哚与靛红很快反应了形成生物学上重要的 3,3 二度(indole-3-yl ) 有高效率和宽底层范围的 indoline-2-(1H ) 。而且,我们证明 p-toluenesulfonic 酸(TsOH ) 能用作一个代理人催化这转变。 | Wenzhong Huang Lingli Nang Xiangguang Li Lin Yuan Yinhai Ma Deqiang Liang | 2015 | Chinese Journal of Chemistry2015，33，10： | 1 |
| 7 | Synthesis and char- acterization of HPMC derivatives as novel duodenum-specific coating agents显示文摘 | Huang Yuan Zheng lingli Liu Jun | 2005 | Archives of Pharmacal Research2005，28，3： | 1 |
| 8 | Optimization of Fermentation Process for Hyaluronic Acid Production by Streptococcus zooepidemicus显示文摘Hyaluronic acid(HA) is a high molecular weight glycosaminoglycan consisting of alternating D-glucuronic acid and N-acetylglucosamine and plays extremely important roles in many biological processes.In this study,we optimized fermentation process for the production of HA by Streptococcus zooepidemicus ATCC35246,including fermentation broth composition and various fermentation parameters.The experimental results showed that the optimal fermentation broth composition was:glucose 45 g/L,yeast extract 10 g/L,tryptone 12 g/L,KH_2PO_42 g/L,K_2HPO_4·3H_2O 2 g/L,MgSO_4 ·7H_2O2 g/L,and(NH_4)_2SO_40.4g/L.The optimal parameters involved in fermentation was:liquid volume 20%,pH 6.0,rotation speed 180 r/min,fermentation temperature 35 °C,fermentation duration 18 h,CTAB concentration 25 mg/L.Under the optimized conditions,the yield of HA was 0.305 g/L,which was dramatically improved by 43.87%compared to that of 0.212 g/L before optimization. | Jing YUAN Zhiping ZHAO Baoqu WANG Lingli WU Li ZHANG | 2015 | Agricultural Biotechnology2015，4，5： | 1 |
| 9 | Acetylation of ezrin regulates membrane–cytoskeleton interaction underlying CCL18-elicited cell migration显示文摘Ezrin,a membrane–cytoskeleton linker protein,plays an essential role in cell polarity establishment,cell migration,and division.Recent studies show that ezrin phosphorylation regulates breast cancer metastasis by promoting cancer cell survivor and promotes intrahepatic metastasis via cell migration.However,it was less characterized whether there are additional post-translational modifications and/or post-translational crosstalks on ezrin underlying context-dependent breast cancer cell migration and invasion.Here we show that ezrin is acetylated by p300/CBP-associated factor(PCAF)in breast cancer cells in response to CCL18 stimulation.Ezrin physically interacts with PCAF and is a cognate substrate of PCAF.The acetylation site of ezrin was mapped by mass spectrometric analyses,and dynamic acetylation of ezrin is essential for CCL18-induced breast cancer cell migration and invasion.Mechanistically,the acetylation reduced the lipid-binding activity of ezrin to ensure a robust and dynamic cycling between the plasma membrane and cytosol in response to CCL18 stimulation.Biochemical analyses show that ezrin acetylation prevents the phosphorylation of Thr567.Using atomic force microscopic measurements,our study revealed that acetylation of ezrin induced its unfolding into a dominant structure,which prevents ezrin phosphorylation at Thr567.Thus,these results present a previously undefined mechanism by which CCL18-elicited crosstalks between the acetylation and phosphorylation on ezrin control breast cancer cell migration and invasion.This suggests that targeting PCAF signaling could be a potential therapeutic strategy for combating hyperactive ezrin-driven cancer progression. | Xiaoyu Song Wanjuan Wang Haowei Wang Xiao Yuan Fengrui Yang Lingli Zhao McKay Mullen Shihao Du Najdat Zohbi Saravanakumar Muthusamy Yalei Cao Jiying Jiang Peng Xia Ping He Mingrui Ding Nerimah Emmett Mingming Ma Quan Wu Hadiyah-Nicole Green Xia Ding Dongmei Wang Fengsong Wang Xing Liu | 2020 | Journal of Molecular Cell Biology2020，12，6： | 1 |
| 10 | Simultaneous determination of malachite green, gentian violet and their leuco-metabolites in shrimp and salmon by liquid chromatography–tandem mass spectrometry with accelerated solvent extraction and auto solid-phase clean-up显示文摘 | Yanfei Tao Dongmei Chen Xiaoqin Chao Huan Yu Pan Yuanhu Zhenli Liu Lingli Huang Yulian Wang Zonghui Yuan | 2011 | Food Control2011，，8： | 1 |
| 11 | Simultaneous determination of malachite green, gentian violet and their leuco-metabolites in shrimp and salmon by liquid chromatography–tandem mass spectrometry with accelerated solvent extraction and auto solid-phase clean-up显示文摘 | Yanfei Tao Dongmei Chen Xiaoqin Chao Huan Yu Pan Yuanhu Zhenli Liu Lingli Huang Yulian Wang Zonghui Yuan | 2011 | Food Control2011，，8： | 1 |
| 12 | Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid-and choline acetyltransferase-positive neurons显示文摘This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells. | Lingli Yu Yindi Ding Ambre Spencer Ji Ma Ruisheng Lu Brian B.Rudkin Chonggang Yuan | 2012 | Neural Regeneration Research2012，7，7： | 1 |
| 13 | Development of a high-performance liquid chromatography method for the simultaneous quantification of quinoxaline-2-carboxylic acid and methyl-3-quinoxaline-2-carboxylic acid in animal tissues显示文摘 | Yujie Wu Huan Yu Yulian Wang Lingli Huang Yanfei Tao Dongmei Chen Dapeng Peng Zhengli Liu Zonghui Yuan | 2006 | Journal of Chromatography A2006，，1： | 1 |
| 14 | Determination of 17 macrolide antibiotics and avermectins residues in meat with accelerated solvent extraction by liquid chromatography–tandem mass spectrometry显示文摘 | Yanfei Tao Gang Yu Dongmei Chen Yuanhu Pan Zhenli Liu Huimin Wei Dapeng Peng Lingli Huang Yulian Wang Zonghui Yuan | 2012 | Journal of Chromatography B2012，，： | 1 |
| 15 | Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats显示文摘 | Shuai Zhang Lingli Zheng Deshi Dong Lina Xu Lianhong Yin Yan Qi Xu Han Yuan Lin Kexin Liu Jinyong Peng | 2013 | Food Chemistry2013，，3： | 1 |
| 16 | Amino acid variation analysis of surface spike glycoprotein at 614 in SARS-CoV-2 strains显示文摘As severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)continues to disperse globally with worrisome speed,identifying amino acid variations in the virus could help to understand the characteristics of it.Here,we studied 489 SARS-CoV-2 genomes obtained from 32 countries from the Nextstrain database and performed phylogenetic tree analysis by clade,country,and genotype of the surface spike glycoprotein(S protein)at site 614.We found that virus strains from China's Mainland were mostly distributed in Clade B and Clade undefined in the phylogenetic tree,with very few found in Clade A.In contrast,Clades A2(one case)and A2a(112 cases)predominantly contained strains from European regions.Moreover,Clades A2 and A2a differed significantly from those of China's Mainland in age of infected population(P Z 0.0071,mean age 40.24 to 46.66),although such differences did not exist between the US and China's Mainland.Further analysis demonstrated that the variation of the Sprotein at site 614(QHD43416.1:p.614D>G)was a characteristic of stains in Clades A2 and A2a.Importantly,this variation was predicted to have neutral or benign effects on the function of the S protein.In addition,global quality estimates and 3D protein structures tended to be different between the two S proteins.In summary,we identified different genomic epidemiology among SARS-CoV-2 strains in different clades,especially in an amino acid variation of the S protein at 614,revealing potential viral genome divergence in SARS-CoV-2 strains. | Canhui Cao Liang Huang Kui Liu Ke Ma Yuan Tian Yu Qin Haiyin Sun Wencheng Ding Lingli Gui Peng Wu | 2020 | Genes & Diseases2020，7，4： | 0 |
| 17 | Recommendations for the Diagnosis and Treatment of Maternal SARS-CoV-2 Infection显示文摘The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has spread worldwide and threatened human’s health.With the passing of time,the epidemiology of coronavirus disease 2019 evolves and the knowledge of SARS-CoV-2 infection accumulates.To further improve the scientific and standardized diagnosis and treatment of maternal SARS-CoV-2 infection in China,the Chinese Society of Perinatal Medicine of Chinese Medical Association commissioned leading experts to develop the Recommendations for the Diagnosis and Treatment of Maternal SARS-CoV-2 Infection under the guidance of the Maternal and Child Health Department of the National Health Commission.This recommendations includes the epidemiology,diagnosis,management,maternal care,medication treatment,care of birth and newborns,and psychological support associated with maternal SARS-CoV-2 infection.It is hoped that the recommendations will effectively help the clinical management of maternal SARS-CoV-2 infection. | Dunjin Chen Yue Dai Xinghui Liu Hongbo Qi Chen Wang Lan Wang Yuan Wei Xiaochao Xu Chuan Zhang Lingli Zhang Yuquan Zhang Ruihua Zhao Yangyu Zhao Borong Zhou Ai-Ling Wang Huixia Yang Li Song | 2023 | Maternal-Fetal Medicine2023，5，2： | 0 |
| 18 | Effects of Polygonatum odoratum Polysaccharide on Reproductive Hormones in Male Rats Fed a High-fat Diet显示文摘[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharide(POP)on organ relative weights and reproductive hormone levels in male rats fed a high-fat diet.[Methods]Thirty healthy male Sprague-Dawley(SD)rats were randomly divided into two groups according to their body weight:10 in normal control group(Group NC,n=10)and 20 in experimental group(n=20).The rats in experimental group were fed a high-fat diet for eight weeks before they were further randomly divided into two groups:high fat group(Group HF)and high fat+400 mg/(kg·d)POP group(Group HF+POP).In Group HF+POP,the rats were administered with POP for another six weeks,before their blood plasma was collected,and the relative weights of their testis and epididymis were calculated.The plasma levels of testosterone(T),estrogen(E2),follicle-stimulating hormone(FSH),cortisol(C)and luteinizing hormone(LH)were measured by radioimmunoassay,and the plasma levels of sex hormone-binding globulin(SHBG)and insulin-like growth factor-1(IGF-1)were determined by enzyme-linked immunosorbent assay.[Results]Compared with Group HF,POP could effectively inhibit rat obesity caused by high-fat diets,increase the relative weights of their testis and epididymis,plasma levels of LH,E2,FSH,T,SHBG and IGF-1,and reduce the plasma level of E2.[Conclusions]Polygonatum odoratum polysaccharide(POP)is able to effectively regulate the level of reproductive hormones in high-fat diet fed rats,and helps to protect their reproductive function. | Yixin YAN Gang ZHANG Lingli BAI Xin TANG Mengjun YUAN Yihong HU Yan WANG Chenzhong JIN | 2023 | Agricultural Biotechnology2023，12，2： | 0 |
| 19 | A 36 Mb terminal deletion of chromosome 2BL is responsible for a wheat semi-dwarf mutation显示文摘Reduced plant height is one of the most important traits related to lodging resistance and crop yield. The use of reduced height genes has been one of the main features in breeding modern high-yielding wheat varieties with less lodging. A spontaneous dwarf mutant DD399 was identified in a high yielding, gibberellic acid(GA)-insensitive, lodging-resistant variety Nongda 399(ND399). Significant differences in upper internode lengths between mutant DD399 and wild type ND399 were caused by reduced cell elongation. The plant height of ND399 × DD399 F_(1) hybrids was intermediate between the parents, indicating incomplete dominance or a dose–response effect of a reduced height gene. Plant height showed continuous distribution in the F_(2) population, and segregation distortion was observed among the 2292 F_(2:3) progenies. The reduced height mutation was characterized by Illumina 90 K iSelect SNP genotyping and bulked segregant RNA-Seq(BSR-Seq) analysis of the segregating population. A concentrated cluster of polymorphic SNPs associated with the reduced height phenotype was detected in the distal region of chromosome arm 2 BL. Co-segregation of reduced height phenotype with the clustered markers revealed a 36 Mb terminal deletion of chromosome 2 BL in mutant DD399. | Qiuhong Wu Yongxing Chen Jingzhong Xie Lingli Dong Zhenzhong Wang Ping Lu Rongge Wang Chengguo Yuan Yan Zhang Zhiyong Liu | 2021 | The Crop Journal2021，9，4： | 0 |
| 20 | Shanghai Autism Early Development:An Integrative Chinese ASD Cohort显示文摘Why Was the Cohort Set Up?Autism spectrum disorder(ASD)is a child neurodevelopmental disorder,the onset of which is generally within 3 years of age,and often leads to lifelong impaired social and cognitive functions,which impose significant mental pressure and economic burdens on the family and society. | Yuan Dai Yuqi Liu Lingli Zhang Tai Ren Hui Wang Juehua Yu Xin Liu Zilin Chen Lin Deng Minyi Tao Hangyu Tan Chu‑Chung Huang Jiaying Zhang Qiang Luo Jianfeng Feng Miao Cao Fei Li | 2022 | Neuroscience Bulletin2022，38，12： | 0 |
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