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| 1 | BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity显示文摘基础编辑() ,包含 cytidine 脱氨基酶和催化地有缺点的 Cas9,广泛地被用来执行基础编辑。然而,狭窄的编辑窗口是限制它的实用程序。这里,我们开发了依照到由熔化 10 的 U (T) 范围(加) 的更大的 C 为招募 GCN4 肽拷贝到 nCas9 (D10A ) 的编程作为基础编辑命名的一种新编辑技术 scFv-APOBEC-UGI-GB1 到目标地点。新系统完成与一扇拓宽的窗户的基础编辑,导致一个增加的指向染色体的范围。有趣地,新系统很产出更少讨厌的 indels 和 non-C-to-T 变换。我们也通过站 codons (iSTOP ) 的正式就职越过整个染色体在基因混乱表明了它的潜在的使用。一起拿,加系统与编辑窗口和提高的忠实增加提供一个新编辑工具。 | Wen Jiang Songjie Feng Shisheng Huang Wenxia Yu Guanglei Li Guang Yang Yajing Liu Yu Zhang Lei Zhang Yu Hou Jia Chen Jieping Chen Xingxu Huang | 2018 | Cell Research2018，28，8： | 20 |
| 2 | Xiaoyaosan improves depressive-like behaviors by regulating the NLRP3 signaling pathway in the rat cerebral cortex显示文摘Objective:To observe changes in the molecular expression of the NLR Family Pyrin Domain Containing Protein 3(NLRP3)pathway in depressed rats after treatment with Xiaoyaosan,and identify the regulatory mechanism of this compound.Methods:Male SpragueeD awley rats were randomly divided into five groups with 12 rats in each group,including the control group,model group,Fluoxetine group,Xiaoyaosan group,and MCC950 group.A depression model was generated by chronic immobilization stress(induced by 3 h of restraint immobilization every day),and the drugs were administered at the same time in each group for 21 days.The effects of Xiaoyaosan on behavioral changes of depressed rats were observed through macroscopic characterization,body mass,open field experiments,and a sucrose preference test.The m RNA and protein expression of the NLRP3 signaling pathway was examined by fluorescence real-time quantitative PCR and Western blot assays.Results:The Xiaoyaosan group,Fluoxetine group,and MCC950 group rats showed improved depressive behavior and an increased weight of sucrose water consumption.The protein and mRNA expression levels of NLRP3,Caspase-1,and IL-1 b were also decreased in the Fluoxetine,Xiaoyaosan,and MCC950 groups.Conclusion:NLRP3,Caspase-1,and IL-1 b protein and mRNA expression levels were increased in the cortex of depressed rats,while Xiaoyaosan protected cortical tissue in these rats by decreasing NLRP3,Caspase-1,and IL-1 b protein and mRNA expression. | Cong Chen Rong Yu Zhe Xue Zhiyi Yan Qinlai Bian Yajing Hou Yunzhi Chen Yueyun Liu Jiaxu Chen | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 5 |
| 3 | 2021 Chinese consensus on the diagnosis and management of primary immune thrombocytopenia in pregnancy显示文摘Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://gffzz16e312a87a7141a5hx6pkuww5o5fn60on.ffgz.tsg.suse.edu.cn/A978). | Zhang Xiaohui Chen Fangping Chen Xiequn Cheng Yunfeng Fang Meiyun Feng Jianming Fu Haixia Gao Hong Han Yue He Aili Hou Ming Hu Yu Huang Ruibin Huang Wenrong Jing Zhicheng Kong Peiyan Liang Aibin Liang Meiying Liu Daihong Liu Junling Liu Lin Liu Xiaowei Ma Liangming Mei Heng Ni Heyu Niu Ting Peng Jun Qiao Jianlin Ren Jinhai Song Yongping Tang Liang V Tong Tong Wang Shaoyuan Wang Xin Wang Zhao Wei Hui Wu Depei Wu Guangsheng Xu Caigang Xu Xue Xu Yajing Yang Linhua Yang Renchi Yang Tonghua Yin Chenghong Yu Li Zhang Guangsen Zhang Lei Zhang Liansheng Zhang Xi Zhao Weili Zhao Yongqiang Zhou Daobin Zhou Hu Zhou Zeping Zhu Tienan Wang Jianliu Huang Xiaojun | 2022 | Chinese Medical Journal2022，，8： | 2 |
| 4 | Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)显示文摘Suspension cells play a crucial role in many biological processes. However, compared to adherent cells, it is particularly challenging to introduce exogenous genes into suspension cells to regulate their biological functions with non-viral gene vectors, mainly due to the low cellular uptake and endosomal escape of polyplexes. Herein, to improve the interactions of polyplexes with cellular membranes, we design and synthesize highly branched poly(β-amino ester)(HPAE) via an “A2 + B4 + C2” Michael addition strategy.Results show that branching significantly increases DNA condensation of HPAE, cellular uptake and endosomal escape of HPAE/DNA polyplexes. In mast cells(MCs), HPAE exhibits up to 80-fold higher gene transfection efficiency compared to the corresponding linear poly(β-amino ester)(LPAE) and the leading commercial gene transfection reagents PEI25k, jetPEI, and Lipofectamine 3000, without causing obvious cytotoxicity. Our study establishes a reliable non-viral platform for efficient gene transfection of suspension cells. | Delu Che Chenfei Wang Zhili Li Kaixuan Wang Shuaiwei Sun Xinyue Zhang Yi Li Zhengju Chen Lei Guo Yajing Hou Dezhong Zhou Songmei Geng | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
| 5 | 3D microgel with extensively adjustable stiffness and homogeneous microstructure for metastasis analysis of solid tumor显示文摘3D microgels with various mechanical properties have been important platforms tumor metastasis analysis,and widely adjustable stiffness is crucial for deeper researches.Herein,by mixing biodegradable polylactic acid(PLA)nanofibers in the modified alginate with different concentrations of Ca^(2+),we significantly enhance the stiffness range of microgels while retaining the pore size,which provides bionic microenvironment for tumor analysis.As a proof of concept,we simulated the mechanical characteristics of breast tumors by encapsulating cells in 3D microgels with diverse stiffness,and analyzed cellular behaviors of two typical breast cancer cell lines:MCF-7 and SUM-159.Results showed that with the addition of 2.0%(w/v)PLA short nanofibers,the Young’s modulus of modified alginate increased more than three-fold.Besides preserving high survival and proliferation rates,both cells also displayed stronger migration ability in soft microgel spheres,where RT-qPCR analysis revealed the underlying changes at the genetic level.This systematic study demonstrated our method is powerful for creating widely adjustable 3D mechanical microenvironment,and the results of cellular behavior analysis shows its promising application prospects in tumorigenesis and progression. | Xiaonan Zheng Ying Hou Qiang Zhang Yajing Zheng Zengnan Wu Xueji Zhang Jin-Ming Lin | 2023 | Chinese Chemical Letters2023，34，11： | 0 |
| 6 | Serving Enterprises and the Public Well显示文摘Can procuring government services be as easy for enterprises and members of the public as buying something online?According to Shanghai’s trademark integrated online government services platform,the answer is a resounding yes!Upon entering the Xuhui Distric t Administrative Service Center,which is the only national-level demonstration zone for the integration of Internet technology with government services in Shanghai,I was greeted by a bright space with a clean design and lots of decorative plants. | Hou Yajing | 2020 | Qiu Shi2020，12，5： | 0 |
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