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| 1 | 四川荥经南罗坝村战国墓显示文摘1988年1月,荥经县塔子山茶场在荥经县附城乡南罗坝村的基建工程中,发现古文化遗物。我馆闻讯后当即派人前往调查,该地为战国时代墓地,在约二百平方米的范围内,我们发掘和清理墓葬十一座(88YLMl—11)。墓地位于经河与烟竹河交汇的二级台地上,北距经河800米,隔河与县城相望。城北即是荥河冲积台地上的同心村巴蜀墓地,两墓地相距仅1公里。墓地东500米为烟竹河,南是打罗坪山,山顶平坦,多为稻田。墓地附近为褐色土,耕土厚27—30厘米,以下即为砂砾层(图一)。 | Yingjing Museum of Yandao City Site | 1994 | 考古学报1994，，3： | 20 |
| 2 | Amyloid beta-peptide worsens cognitive impairment following cerebral ischemia-reperfusion injury显示文摘Amyloid β-peptide,a major component of senile plaques in Alzheimer’s disease,has been implicated in neuronal cell death and cognitive impairment.Recently,studies have shown that the pathogenesis of cerebral ischemia is closely linked with Alzheimer’s disease.In this study,a rat model of global cerebral ischemia-reperfusion injury was established via occlusion of four arteries;meanwhile,fibrillar amyloidβ-peptide was injected into the rat lateral ventricle.The Morris water maze test and histological staining revealed that administration of amyloidβ-peptide could further aggravate impairments to learning and memory and neuronal cell death in the hippocampus of rats subjected to cerebral ischemia-reperfusion injury.Western blot showed that phosphorylation of tau protein and the activity of glycogen synthase kinase 3βwere significantly stronger in cerebral ischemia-reperfusion injury rats subjected to amyloid β-peptide administration than those undergoing cerebral ischemia-reperfusion or amyloidβ-peptide administration alone.Conversely,the activity of protein phosphatase 2A was remarkably reduced in rats with cerebral ischemia-reperfusion injury following amyloidβ-peptide administration.These findings suggest that amyloidβ-peptide can potentiate tau phosphorylation induced by cerebral ischemia-reperfusion and thereby aggravate cognitive impairment. | Bo Song Qiang Ao Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，26： | 5 |
| 3 | In vitro biocompatibility of three chitosan/polycation composite materials for nerve regeneration显示文摘BACKGROUND:It has been reported that chitosan nerve conduits could support axon elongation and improve relevant function during in vivo nerve regeneration. OBJECTIVE: To investigate in vitro biocompatibility of three novel, chitosan/polycation composite materials for nerve regeneration in cultured mouse Schwann cells and PC12 cells. DESIGN, TIME AND SETTING: The observational, control experiments for nerve tissue engineering were performed at the Department of Biological Sciences and Biotechnology of Tsinghua University from August 2007 to January 2008. MATERIALS: Mouse Schwann cells were isolated from the sciatic nerve of 5–7-day-old BALB/C mice. PC12 cells were purchased from the American Type Culture Collection (ATCC, USA). Chitosan was purchased from Tsingdao Haisheng Co., China. Poly-L-lysine hydrochloride (PLL), polyethyleneimine (PEI) poly-L-ornithine hydrobromide (POR), and S-100 antibody was purchased from Sigma Chemical Co., USA. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Chemical Co., Japan. METHODS: Three chitosan/polycation composite materials for nerve regeneration (PLL-0.25, PEI-0.25, and POR-0.25) were produced by blending chitosan with 0.25% (w/w) poly-L-lysine, polyethyleneimine, and poly-L-ornithine. Pure chitosan was utilized as the control. After 3 days of culture, the morphology of mouse Schwann and PC12 cells cultured on all substrates was observed with an inverted phase contrast microscope. Mouse Schwann cells were stained by immunofluorescence labeling S-100 protein and nuclei, followed by identification with a confocal laser-scanning microscope. The amount of proliferating mouse Schwann and PC12 cells was determined by CCK-8 after 1, 3, and 5 days in culture. The level of PC12 cell differentiation on all substrates was assessed by measuring neurite length at 1, 3, and 5 days after seeding. MAIN OUTCOME MEASURES: Morphology and amount of proliferation of mouse Schwann cells and PC12 cells cultured on chitosan and three polycation-modified materials, as well as amount of differentiation in PC12 cells on these substrates. RESULTS: (1) Morphology of mouse Schwann cells and PC12 cells on all substrates: after 3 days in culture on three different chitosan/polycation composite substrates, Schwann cells were connected to each other and exhibited greater proliferation, compared to the chitosan control. In particular, on PLL-0.25 and POR-0.25 substrates, some cells congregated and nearly reached confluence. The PC12 cells on chitosan substrate, after 3 days in culture, maintained a round shape; few exhibited a bipolar shape and began to form neurite extensions. However, on PLL-0.25 and POR-0.25 substrates, most PC12 cells displayed a bipolar shape with obvious neurite outgrowth, and almost grew as an adherent, spreading monolayer. (2) Proliferation of mouse Schwann cells and PC12 cells on all substrates: on the first day, Schwann cell proliferation on the three composite substrates was significantly greater than the cells on chitosan control (P < 0.01). After 3 and 5 days in culture, PLL-0.25 and POR-0.25 substrates resulted in greater cell proliferation when compared to pure chitosan (P < 0.01). On the third and fifth day in culture PC12 cell proliferation on PLL-0.25 and POR-0.25 was significantly greater than on chitosan substrate (P < 0.01). (3) Differentiation of PC12 cells on all substrates: at all time points, the average neurite length of cells cultured on composite materials was significantly longer than on chitosan control (P < 0.05-0.01). Cells on PLL-0.25 exhibited the longest average neurite length at days 3 and 5. CONCLUSION: Mouse Schwann cells and PC12 cells exhibit in vitro biocompatibility with poly-L-lysine-and poly-L-ornithine-modified substrates, which indicates that these substrates could serve as suitable substrates for peripheral nerve regeneration. | Zhenhuan Zheng Yujun Wei Gan Wang Yandao Gong Xiufang Zhang | 2008 | Neural Regeneration Research2008，3，8： | 2 |
| 4 | The mitochondrial function was impaired in APP knockout mouse embryo fibroblast cells显示文摘The amyloid precursor protein (APP) is recognized as the source of Aβ, which plays an important role in Alzheimer’s disease. However, the biological function of APP is obscure. Previous studies showed that mitochondria could be a target of APP. In this work, APP knockout mouse embryo fibroblast (MEF) cells were used to test if APP plays any role in maintaining the mitochondrial function. As the result, APP knockout MEF cells (APP-/- cells) showed the abnormal mitochondrial function, including slower cell proliferation, lower mitochondrial membrane potential, lower intracellular ROS, higher mitochondrial membrane fluidity and lower cytochrome c oxidase activity than their wild-type counterparts. However, no change was found in the amount of mitochondria in MEF APP-/-cells. | SHENG BaiYang NIU Ying ZHOU Hui ZHOU Hui YAN JiaXin ZHAO NanMing ZHANG XiuFang GONG YanDao | 2009 | Chinese Science Bulletin2009，54，10： | 2 |
| 5 | Phosphorylation of tau protein over time in rats subjected to transient brain ischemia显示文摘Transient brain ischemia has been shown to induce hyperphosphorylation of the microtubule-associated protein tau.To further determine the mechanisms underlying these processes,we investigated the interaction between tau,glycogen synthase kinase(GSK)-3βand protein phosphatase 2A.The results confirmed that tau protein was dephosphorylated during brain ischemia;in addition,the activity of GSK-3βwas increased and the activity of protein phosphatase2A was decreased.After reperfusion,tau protein was hyperphosphorylated,the activity of GSK-3βwas decreased and the activity of protein phosphatase 2A remained low.Importantly,the interaction of tau with GSK-3βand protein phosphatase 2A was altered during ischemia and reperfusion Lithium chloride could affect tau phosphorylation by regulating the interaction of tau with GSK-3βand protein phosphatase 2A,and improve learning and memory ability of rats after transient brain ischemia.The present study demonstrated that it was the interaction of tau with GSK-3βand protein phosphatase 2A,rather than their individual activities,that dominates the phosphorylation of tau in transient brain ischemia.Hyperphosphorylated tau protein may play an important role in the evolution of brain injury in ischemic stroke.The neuroprotective effects of lithium chloride partly depend on the inhibition of tau phosphorylation during transient brain ischemia. | Bo Song Qiang Ao Zhen Wang Weiqiang Liu Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，34： | 2 |
| 6 | Suppression of GSK3β by ERK mediates lipopolysaccharide induced cell migration in macrophage through β-catenin signaling显示文摘We investigate the role of β-catenin signaling in the response of macrophage to lipopolysaccharide(LPS)using RAW264.7 cells.LPS rapidly stimulated cytosolic β-catenin accumulation.β-catenin-mediated transcription was showed to be required for LPS induced gene expression and cell migration.Mechanically,ERK activation-primed GSK3β inactivation by Akt was demonstrated to mediate the LPS induced β-catenin accumulation.Overall,our findings suggest that suppression of GSK3β by ERK stimulatesβ-catenin signaling therefore contributes to LPS induced cell migration in macrophage activation. | Kai Gong Fangfang Zhou Huizhe Huang Yandao Gong Long Zhang | 2012 | Protein & Cell2012，3，10： | 2 |
| 7 | Studies on nerve cell affinity of biodegradable modified chitosan films显示文摘 | Mingyu Cheng Wenling Cao Yuan Gao Yandao Gong Nanming Zhao Xiufang Zhang | 2003 | Journal of Biomaterials Science Polymer Edition2003，，10： | 1 |
| 8 | Properties and biocompatibility of chitosan films modified by blending with PEG显示文摘 | Zhang Miao Li Xunhu Gong Yandao Zhao Nanming Zhang Xiufang | | 0，，： | 1 |
| 9 | Investigation of MC3T3- E1 cell behavior on the surface of GRGDS-coupled chitosan显示文摘 | Li Jing Yun Huan Gong Yandao | 2006 | Biomacromolecules2006，7，4： | 1 |
| 10 | Studies on nerve cell affinity of biodegradable modified chitosan films显示文摘 | Mingyu Cheng Wenling Cao Yuan Gao Yandao Gong Nanming Zhao Xiufang Zhang | 2003 | Journal of Biomaterials Science Polymer Edition2003，，10： | 1 |
| 11 | Endogenously generated amyloid β increases membrane fluidity in neural 2a cells显示文摘The effect of endogenously generated amyloid β on membrane fluidity was investigated in Neural 2a cells stably expressing Swedish mutant amyloid precursor protein (APPswe). Membrane fluidity was studied by fluorescence polarizability using 1,6-Diphenyl-1,3,5-Hexatriene (DPH) as the fluorescence probe. It was found that the membrane fluidity in APPswe cells was significantly higher than that in its wild type counterparts. Alleviating the effect of amyloid β either by γ secretase activity inhibition or by amyloid antibody treatment decreased membrane fluidity, which indicated an important role of amyloid β in increasing membrane fluidity. Treatment using amyloid β channel blocker, tromethamine and NA4 suggested that channel formed by amyloid β on the cell membrane is a way through which amyloid β takes its membrane fluidizing effect. | NIU Ying SHENG BaiYang SONG Bo LIU LingLing ZHANG XiuFang ZHAO NanMing GONG YanDao | 2009 | Chinese Science Bulletin2009，54，3： | 1 |
| 12 | Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions显示文摘 | Mingyu Cheng Jinguang Deng Fei Yang Yandao Gong Nanming Zhao Xiufang Zhang | 2003 | Biomaterials2003，，17： | 1 |
| 13 | Physical, mechanical and degradation properties, and Schwann cell affinity of cross-linked chitosan films显示文摘 | Wenling Cao Mingyu Cheng Qiang Ao Yandao Gong Nanming Zhao Xiufang Zhang | 2005 | Journal of Biomaterials Science Polymer Edition2005，，6： | 1 |
| 14 | The effect of glutaraldehyde treatment on collagen structure显示文摘 | Xing Rongzhi Gong Yandao Zhang Xiufang | 1998 | Acta Biophysica Sinica1998，14，1： | 1 |
| 15 | Degradation of covalently cross-linked carboxymethyl chitosan and its potential application for peripheral nerve regeneration显示文摘 | Guangyuan Lu Lijun Kong Baiyang Sheng Gan Wang Yandao Gong Xiufang Zhang | 2007 | European Polymer Journal2007，，9： | 1 |
| 16 | Simulation for the primary electronic donor of photosystem II in vitro显示文摘Histidine coordinated to Chl a is a distinct characteristic of Chl a in vivo. By usinghistidine analogue of 1-methylimidazole (C4H6N2) and measuring the UV/vis absorption, CD and MCD spectra of the interaction between C4H6N2and Chl a in CCI4, we have obtained that: (i) In pure CCI4 solvent, Chl a molecule is in five-coordinate state, and two Chl a molecules form an asymmetric compact-dimer with strong coupling interaction. We propose that the two Chl a molecules are connected by two unequally coordinated Mg-O bonds (the two oxygen atoms come from the C=O of C131 keto and C17 ester, respectively); (ii) when the molar ratio of C4H6N2/Chl a is 0.5 or 1 (corresponding to 2Chl a #1C4H6N2 and 2Chl a ·2C4H6N2, respectively), significant changes have been observed in the absorption, CD and MCD spectra, which indicate that the Chl a remains in dimer form, but the coupling interaction between them reduces greatly. We postulate that C4H6N2 replaces the ligation of C=O of C17 ester and C131 keto to Mg atoms | PAN Jie ZHANG Chunxi GONG Yandao LI Liangbi ZHAO Nanming KUANG Tingyun | 2000 | Chinese Science Bulletin2000，45，10： | 0 |
| 17 | Dithiothreitol decreases the thermal stability and unfolding cooperativity of ribulose-1,5-bisphosphate carboxylase/oxygenase显示文摘Plant rubisco consists of eight large subunits (55 kD) encoded by chloroplast gene and eight small subunits (15 kD) encoded by nuclear gene. There are abundant cysteine residues that do not form disulfide bonds in native rubisco. Differential scanning calorimetry has been used to study some plant rubisco and suggested an irreversible two-state denaturation due to the high cooperativity in subunits. By comparing the data from circular dichroism, fluorescence, differential scanning calorimetry, SDS electrophoresis, and activity assays in the absence or presence of DTT, we suggest that the formation of disulfide bonds in subunits during the early thermal unfolding may increase the thermal stability and the thermal unfolding cooperativity of rubisco. | LI Guofu, MAO Haibin, LI Donghai, GONG Yandao, ZHANG Xiufang and ZHAO Nanming(State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua U-niversity, Beijing 100084, China) | 2003 | Progress in Natural Science:Materials International2003，13，3： | 0 |
| 18 | Amyloid precursor protein-mediated modulation of capacitive calcium entry显示文摘Alzheimer's disease(AD) is a progressive neurodegenerative disorder.In the present study,the function of amyloid precursor protein(APP) in modulating capacitive calcium entry(CCE),a refilling mechanism for depleted intracellular calcium stores,was investigated.CCE in neural 2a(N2a) cells stably expressing wild-type human APP was lower than in wild-type N2a cells,while CCE in APP knockout mouse embryonic fibroblast(MEF) cells was higher than in their wild-type counterparts.We demonstrate that wild-type APP depresses CCE.Furthermore,using N2a cells transfected with C-terminal APP fragments,we show that these fragments anchored in the cell membrane play an important role in CCE depression. | MA Tuo NIU Ying ZHAO Chang SU ZuQi AO Qiang ZHANG XiuFang ZHAO NanMing GONG YanDao | 2012 | Chinese Science Bulletin2012，57，35： | 0 |
| 19 | The photodamage process of pigments and proteins of PSI complexes from Spinacia Oleracea L.显示文摘Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction | WEI Jie YU Hui LI Liangbi KUANG Tingyun WANG Jushuo GONG Yandao ZHAO Nanming | 2001 | Chinese Science Bulletin2001，46，21： | 0 |
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