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| 1 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 2 | Joint Source-Channel Coding for 6G Communications显示文摘In order to provide ultra low-latency and high energy-efficient communication for intelligences,the sixth generation(6G)wireless communication networks need to break out of the dilemma of the depleting gain of the separated optimization paradigm.In this context,this paper provides a comprehensive tutorial that overview how joint source-channel coding(JSCC)can be employed for improving overall system performance.For the purpose,we first introduce the communication requirements and performance metrics for 6G.Then,we provide an overview of the source-channel separation theorem and why it may not hold in practical applications.In addition,we focus on two new JSCC schemes called the double low-density parity-check(LDPC)codes and the double polar codes,respectively,giving their detailed coding and decoding processes and corresponding performance simulations.In a nutshell,this paper constitutes a tutorial on the JSCC scheme tailored to the needs of future 6G communications. | Yanfei Dong Jincheng Dai Kai Niu Sen Wang Yifei Yuan | 2022 | China Communications2022，19，3： | 2 |
| 3 | miR-3677-3p promotes hepatocellular carcinoma progression via inhibiting GSK3β显示文摘MicroRNAs play im portant roles in regulating hepatocellular carcinoma(HCC)form ation,progression and metastasis.However,their functions and the underlying m olecular mechanisms are still unclear.Here,we found that miR-3677-3p was highly expressed in prim ary tumor tissues of HCC patients.And its inhibition by using sponge in HCC cells could suppress cell proliferation sig nificantly,but it has no effect on cell apoptosis.Through directly targeting to the 37 untranslated region of glycogen synthase kinase 3-β(GSK3β),miR-3677-3p could inhibit GSK3β expression.Our study revealed that the miR-3677-3p/GSK3β axis may play a crucial role in HCC and miR-3677-3p may serve as a potential diagnostic biom arker or a therapeutic target for HCC. | Yanfei Li Yajie Zhou Linlin Ma Dingsheng Liu Zhensheng Dai Junwei Shen | 2020 | Acta Biochimica et Biophysica Sinica2020，52，12： | 1 |
| 4 | Thermal stability of double-ceramic-layer thermal barrier coatings with various coating thickness显示文摘 | Hui Dai Xinghua Zhong Jiayan Li Yanfei Zhang Jian Meng Xueqiang Cao | 2006 | Materials Science & Engineering A2006，，1： | 1 |
| 5 | Tobacco Rattle Virus-induced Phytoene Desaturase (PDS) Silencing in Centaurea cyanus显示文摘Virus-induced gene silencing(VIGS)is a genetic tool used to assess gene function.Tobacco rattle virus(TRV)is a VIGS vector commonly used to induce endogenous gene silencing in plants.However,there is no VIGS system established for Centaurea spp.We evaluated the effectiveness of a TRV-based VIGS system using phytoene desaturase(PDS)as a reporter gene in Centaurea cyanus.Three methods including pressure-,vacuum-and apical meristem-infiltrationwere tested to infect C.cyanus seedlings.Photobleached leaveswere only obtained using apicalmeristem-infiltration after a 14 d treatment.The CcPDS transcripts in photobleached leaves were significantly reduced compared with that in green leaves treated with empty TRV.Four C.cyanus cultivars were tested to detect their VIGS responses,and‘Dwarf Tom Pouce Blue’was the most sensitive.The agro-infiltration condition was optimized by screening for the optimal seedling stage as well as the optimum Agrobacterium density for efficient silencing.Seedlings with four true leaves and infiltration with an Agrobacterium density of OD_(600)0.5 were optimal conditions to obtain more photobleached leaves and more intense photobleached phenotype.The results demonstrated the feasibility of TRV-based VIGS for functional analysis of genes in C.cyanus. | Chengyan Deng Fan Zhang Jiaying Wang Yanfei Li He Huang Silan Dai | 2021 | Horticultural Plant Journal2021，7，2： | 1 |
| 6 | A primer-initiated strand displacement amplification strategy for sensitive detection of 5-Hydroxymethylcytosine in genomic DNA显示文摘5-Hydroxymethylcytosine(5 hmC),an intermediate product of DNA demethylation,is important for the regulation of gene expression during development and even tumorigenesis.The challenges associated with determination of 5 hm C level include its extremely low abundance and high structural similarity with other cytosine derivatives,which resulted in sophisticated treatment with large amount of sample input.Herein,we developed a primer-initiated strand displacement amplification(PISDA)strategy to quantify the global 5 hm C in genomic DNA from mammalian tissues with high sensitivity/selectivity,low input and simple operation.This sensitive fluorescence method is based on 5 hmC-specific glucosylation,primer ligation and DNA amplification.After the primer was labeled on 5 hm C site,DNA polymerase and nicking enzyme will repeatedly act on each primer,causing a significant increase of fluorescence signal to magnify the minor difference of 5 hm C content from other cytosine derivatives.This method enables highly sensitive analysis of 5 hm C with a detection limit of 0.003%in DNA(13.6 fmol,S/N=3)from sample input of only 150 ng,which takes less than 15 min for determination.Further determination of 5 hmC in different tissues not only confirms the widespread presence of 5 hmC but also indicates its significant variation in different tissues and ages.Importantly,this PISDA strategy exhibits distinct advantages of bisulfite-free treatment,mild conditions and simple operation without the involvement of either expensive equipment or large amount of DNA sample.This method can be easily performed in almost all research and medical laboratories,and would provide a promising prospect to detect global 5 hmC in mammalian tissues. | Yunda Li Yanfei Zhang Zhenning Yu Yuzhi Xu Si-Yang Liu Zong Dai Xiaoyong Zou | 2022 | Chinese Chemical Letters2022，33，8： | 0 |
| 7 | Edge preparation methods for cutting tools:a review显示文摘Edge preparation can remove cutting edge defects,such as burrs,chippings,and grinding marks,generated in the grinding process and improve the cutting performance and service life of tools.Various edge preparation methods have been proposed for different tool matrix materials,geometries,and application requirements.This study presents a scientific and systematic review of the development of tool edge preparation technology and provides ideas for its future development.First,typical edge characterization methods,which associate the microgeometric characteristics of the cutting edge with cutting performance,are briefly introduced.Then,edge preparation methods for cutting tools,in which materials at the cutting edge area are removed to decrease defects and obtain a suitable microgeometry of the cutting edge for machining,are discussed.New edge preparation methods are explored on the basis of existing processing technologies,and the principles,advantages,and limitations of these methods are systematically summarized and analyzed.Edge preparation methods are classified into two categories:mechanical processing methods and nontraditional processing methods.These methods are compared from the aspects of edge consistency,surface quality,efficiency,processing difficulty,machining cost,and general availability.In this manner,a more intuitive understanding of the characteristics can be gained.Finally,the future development direction of tool edge preparation technology is prospected. | Yu ZHOU Wei FANG Lanying SHAO Yanfei DAI Jiahuan WANG Xu WANG Julong YUAN Weigang GUO Binghai LYU | 2023 | Frontiers of Mechanical Engineering2023，18，4： | 0 |
| 8 | Microgravity Material Research in China:2012–2014显示文摘During 2012–2014, the main research activities from microgravity material research were focused on, which include study of microgravity effects on collagen fibrillogenesis and HAP crystallization, microgravity experiments using drop tube, and research of thermoelectric materials for space.This paper summarizes all these activities. | WANG Yuren DAI Guoliang WANG Jing FENG Shaobo LUO Xinghong ZHOU Yanfei CHEN Lidong YU Yude | 2014 | 空间科学学报2014，34，5： | 0 |
| 9 | Epitaxial growth ofβ-Ga_(2)O_(3)thin films on Ga_(2)O_(3)and Al_(2)O_(3)substrates by using pulsed laser deposition显示文摘In this work,we have successfully grown high quality epitaxialβ-Ga_(2)O_(3)thin films onβ-Ga_(2)O_(3)(100)and Al_(2)O_(3)(0001)substrates using pulsed laser deposition(PLD).By optimizing temperature and oxygen pressure,the best conditions were found to be 650-700℃and 0.5 Pa.To further improve the quality of hetero-epitaxialβ-Ga_(2)O_(3),the sapphire substrates were pretreated for atomic terraced surface by chemical cleaning and high temperature annealing.From the optical transmittance measurements,the films grown at 600-750℃exhibit a clear absorption edge at deep ultraviolet region around 250-275 nm wavelength.High resolution transmission electron microscope(HRTEM)images and X-ray diffraction(XRD)patterns demonstrate thatβ-Ga_(2)O_(3)(-201)//Al_(2)O_(3)(0001)epitaxial texture dominated the epitaxial oxide films on sapphire substrate,which opens up the possibilities of high power electric devices. | Yuxin An Liyan Dai Ying Wu Biao Wu Yanfei Zhao Tong Liu Hui Hao Zhengcheng Li Gang Niu Jinping Zhang Zhiyong Quan Sunan Dingy | 2019 | Journal of Advanced Dielectrics2019，9，4： | 0 |
| 10 | Generation of monoclonal antibodies against n-3 fatty acid desaturase显示文摘Dear Editor:Omega-3 polyunsaturated fatty acids(n-3 PUFAs)are essential fatty acids for normal cellular functions and have been used for prevention and treatment of many diseases,including coronary heart disease,diabetes,and cancers[1-3].n-3 PUFAs and n-6 PUFAs have been shown to decrease and increase the severity of several human diseases,respectively[4].Unfortunately,mammals have no enzymes to synthesize n-6 and | Yanfei Zhang Yilin Yuan Meilei Sheng Wei Zhang Ying Wang Xiao Zhang Yifan Dai | 2015 | The Journal of Biomedical Research2015，29，6： | 0 |
| 11 | Two-dimensional coordination polymer-based nanosensor for sensitive and reliable nucleic acids detection in living cells显示文摘A reliable and sensitive strategy which can assess nucleic acid levels in living cells would be essential for fundamental research of biomedical applications. Some nanomaterial-based fluorescence biosensors recently developed for detecting nucleic acids, however, are often with expensive, complicated and timeconsuming preparation process. Here, by using a facile bottom-up synthesis method, a two-dimensional(2 D) coordination polymer(CP) nanosheet, [Cu(tz)](Htz = 1,2,4-triazole), was successfully prepared after optimizing reaction conditions. These ultrathin CP nanosheets with thickness of 4.7 ± 1.1 nm could readily form nanosensors by assembly with DNA probes, which exhibited a low limit of detection(LOD)for p53 DNA fragment as 144 pmol/L. Furthermore, by integrating [Cu(tz)] nanosheets with hybridization chain reaction(HCR) probes, mi R-21, one kind of micro RNA upregulated in many cancer cells, can be sensitively detected with a LOD of 100 pmol/L and monitored in living cells, giving consistent results with those obtained by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) analysis.Thus [Cu(tz)] nanosheets, which not only possess much better nucleic acids sensing performance than bulk cystals, but also exhibit nucleic acid delivery functions, could be used as a novel nanoplatform in biomedical imaging and sensing applications. | Yuzhi Xu Yanfei Zhang Huihui Yang Wen Yin Leli Zeng Shuo Fang Si-Yang Liu Zong Dai Xiaoyong Zou Yihang Pan | 2022 | Chinese Chemical Letters2022，33，2： | 0 |
| 12 | RPA3 is transcriptionally activated by YY1 and its depletion enhances radiosensitivity of triple-negative and HER2-positive breast cancer显示文摘RPA3(Replication Protein A3)(14 kD)is a part of the canonical heterotrimeric replication protein A complex(RPA/RP-A).This study aimed to explore the functional role of RPA3 and the mechanisms of its dysregulation in breast cancer.Data from the Cancer Genome Atlas(TCGA)-breast cancer patients and GSE75688 were utilized for gene expression and survival analysis.Breast cancer cell lines MDA-MB-231 and SK-BR-3 were used for in-vitro cell studies.Clonogenic assay and immunofluorescent staining ofγ-H2AX were performed to examine radiation-induced cytotoxicity.Systemic correlation analysis was performed to identify potential transcription factors(TFs)regulating RPA3 expression.ChIP-qPCR and dual-luciferase assay were conducted to verify the transcriptional activating effect of YY1 on RPA3 expression.Bioinformatic analysis showed that RPA3 expression was upregulated in breast cancer.Its upregulation was associated with poor survival of basal-like and HER2+cases.RPA3 inhibition by siRNA reduced colony formation and increasedγ-H2AX foci formation after irradiation in MDA-MB-231 and SK-BR-3 cells.RPA3 expression was transcriptionally activated by YY1 via promoter binding in the two cell lines.Both RPA3 and YY1 expression were positively correlated with their gene-level copy numbers.RPA3 might serve as a potential target for radio-sensitization in basal-like and HER2+breast cancer. | YANFEI LI LULU DAI KE CAI YINGKUI SONG XIQING LIU | 2021 | BIOCELL2021，45，3： | 0 |
| 13 | A photo-elutable and template-free isothermal amplification strategy for sensitive fluorescence detection of 5-formylcytosine in genomic DNA显示文摘5-Formylcytosine(5fC), as an important epigenetic modification, plays a vital role in diverse biological processes and multiple diseases by regulating gene expression. Owing to the extremely low abundance of 5fC in all mammalian tissues and high structural similarity with other cytosine derivatives, the precise and sensitive detection of 5fC is challenging. Herein, a photo-elutable and template-free isothermal amplification strategy has been proposed for the sensitive detection of 5fC in genomic DNA based on5fC-specific biotinylation, enrichment, photocleavage, and terminal deoxynucleotidyl transferase(Td T)-assisted fluorescence signal amplification, which is termed 5fC-PTIAS. By introducing the highly specific chemolabeling and the one-step photoelution processes, this strategy possesses a minimal nonspecific background as well as a much higher amplification efficiency. With the high signal-to-noise ratio, this strategy can achieve the accurate quantification of 5fC in various biological samples including mouse brain, kidney, and liver, with a limit of detection(LOD) of 0.025‰ in DNA(S/N=3). These results not only confirm the widespread distribution of 5fC but also indicate its significant variation in different tissues and ages. The bisulfite-and mass spectrometry-free strategy is highly sensitive, selective, and easily mastered, holding great promise in detecting other epigenetic modifications with much lower levels. | Hongling Yang Yanfei Zhang Zhenning Yu Si-Yang Liu Yuzhi Xu Zong Dai Xiaoyong Zou | 2023 | Chinese Chemical Letters2023，34，3： | 0 |
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