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| 1 | Overexpression of Dickkopf-3 induces apoptosis through mitochondrial pathway in human colon cancer显示文摘AIM:To investigate the mechanisms of the biological roles of Dickkopf-3(Dkk-3) in cell invasion,survival and apoptosis in colon cancer cells.METHODS:Three human colon cancer cell lines,i.e.,HT-29,LoVo and SW480,were used.Overexpression of Dkk-3 induced by pEGFP-N1-Dkk-3-GFP plasmid in LoVo cells was performed using Lipofectamine 2000 reagent.Reverse transcription polymerase chain reaction and Western blotting were performed to determine the mRNA and protein expression levels of Dkk-3,respectively.Cell proliferation assay,cell cycle analysis,hoechst 33258 assay and Matrigel invasion assay were performed on Dkk-3 overexpressing transfectants.RESULTS:The mRNA and protein expressions of Dkk-3 in HT-29(mRNA:0.06 ± 0.02,protein:0.06 ± 0.01) and LoVo(mRNA:0.07 ± 0.02,protein:0.07 ± 0.02) cells were significantly lower than that in SW480 cells(mRNA:0.92 ± 0.04,protein:0.69 ± 0.13;all P < 0.05),and the greatest levels of invasiveness wasin LoVo cells.Dkk-3 overexpression inhibited the proliferation and invasion of LoVo cells and induced cell cycle arrest at G0/G1 phase and subsequent apoptosis,as indicated by increased chromatin condensation and fragments,upregulated Bax and cytochrome c protein,downregulated survivin and Bcl-2 protein,and the activation of caspase-3 and caspase-9.Furthermore,Dkk-3 overexpression reduced the accumulation of cytosolic fraction of β-catenin.CONCLUSION:Dkk-3 overexpression induced apoptosis in human colon cancer possibly through the mitochondrial pathway.Dkk-3 may be involved in the Wnt/β-catenin signaling pathways in colon cancer. | Zi-Rong Yang Wei-Guo Dong Xiao-Fei Lei Meng Liu QiSheng Liu | 2012 | World Journal of Gastroenterology2012，18，14： | 7 |
| 2 | Observation and research of deep underground multi-physical fields—Huainan–848 m deep experiment显示文摘Compared with the surface,the deep environment has the advantages of allowing“super-quiet and ultra-clean”-geophysical field observation with low vibration noise and little electromagnetic interference,which are conducive to the realization of long-term and high-precision observation of multi-physical fields,thus enabling the solution of a series of geoscience problems.In the Panyidong Coal Mine,where there are extensive underground tunnels at the depth of 848 m below sea level,we carried out the first deep-underground geophysical observations,including radioactivity,gravity,magnetic,magnetotelluric,background vibration and six-component seismic observations.We concluded from these measurements that(1)the background of deep subsurface gravity noise in the long-period frequency band less than 2 Hz is nearly two orders of magnitude weaker than that in the surface observation environment;(2)the underground electric field is obviously weaker than the surface electric field,and the relatively high frequency of the underground field,greater than 1 Hz,is more than two orders of magnitude weaker than that of the surface electric field;the east-west magnetic field underground is approximately the same as that at the surface;the relatively high-frequency north-south magnetic field underground,below 10 Hz,is at least one order of magnitude lower than that at the surface,showing that the underground has a clean electromagnetic environment;(3)in addition to the highfrequency and single-frequency noises introduced by underground human activities,the deep underground space has a significantly lower background vibration noise than the surface,which is very beneficial to the detection of weak earthquake and gravity signals;and(4)the underground roadway support system built with ferromagnetic material interferes the geomagnetic field.We also found that for deep observation in the“ultra-quiet and ultra-clean”environment,the existing geophysical equipment and observation technology have problems of poor adaptability and insufficient precision as well as data cleaning problems,such as the effective separation of the signal and noise of deep observation data.It is also urgent to interpret and comprehensively utilize these high-precision multi-physics observation data. | Yun WANG Yaxin YANG Heping SUN Chengliang XIE Qisheng ZHANG Xiaoming CUI Chang CHEN Yongsheng HE Qiangqiang MIAO Chaomin MU Lianghui GUO Jiwen TENG | 2023 | Science China Earth Sciences2023，66，1： | 3 |
| 3 | Spatial distribution and reconstruction potential of Japanese anchovy(Engraulis japonicus) based on scale deposition records in recent anaerobic sediment of the Yellow Sea and East China Sea显示文摘Many studies have revealed that anchovy has exhibited large variability in population size on decadal timescales. However, such works concerning anchovy population are mainly based on short historical catch records. In order to understand the causes of variability in fish stocks(natural and/or anthropogenic) and calibrate the error between catches and standing stocks, it is essential to develop long-term time series of fish stocks from the time when human impacts are minimal or negligible. Well preserved fish scales from sediment record are regarded as useful indicators revealing the history of fish population dynamics over the last centuries. Anchovy scales was first analyzed over the Yellow Sea and East China Sea and the largest abundance was found in the central South Yellow Sea where is regarded as the largest overwintering ground for Japanese anchovy(Engraulis japonicas). Thus in the central South Yellow Sea, two cores covering the last 150 years were collected for estimating fish scale flux. The scale deposition rate(SDR) records show that the decadal scale SDRs were obviously coherent between cores with independent chronologies. The calibration of downcore SDRs to the standing stocks of anchovy further validated that SDR is a reliable proxy to reconstruct the long-term anchovy population dynamic in the central South Yellow Sea where anoxic conditions prevail in the sediment. When assembled with other productivity proxies, it would be expected that SDR could be associated with changes in oceanic productivity and may make a contribution to determine the forcing factors and elucidate the mechanism of the process in future. | HUANG Jiansheng SUN Yao JIA Haibo YANG Qian TANG Qisheng | 2014 | Acta Oceanologica Sinica2014，33，12： | 2 |
| 4 | Effects of fish meal replacement with Chlorella meal on growth performance,pigmentation,and liver health of largemouth bass(Micropterus salmoides)显示文摘Chlorella meal is a potential protein source for aquafeeds.However,the physiological response of carnivorous fish fed Chlorella meal remains elusive.This study evaluated the effects of replacing dietary fish meal with Chlorella meal on growth performance,pigmentation,and liver health in largemouth bass.Five diets were formulated to replace dietary fish meal of 0%(C0,control),25%(C25),50%(C50),75%(C75),and 100%(C100)with Chlorella meal,respectively.Total 300 fish(17.6±0.03 g)were randomly assigned to 15 tanks(3 tanks/group).Fish were fed the experimental diet twice daily for 8 weeks.The increased dietary Chlorella meal quadratically influenced the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and feed intake(FI),which were significantly lower in the C100 group than in the other groups(P<0.05).The feed conversion ratio(FCR)increased linearly or quadratically with dietary Chlorella meal.Dietary Chlorella meal linearly or quadratically increased the lutein content of plasma,liver,and dorsal muscle of largemouth bass(P<0.05).Compared to the C0 group,all supplemented Chlorella meal groups significantly improved the yellowness(b*)of the dorsal body(1.5 to 2.0 fold),abdominal body(1.5 to 1.8 fold),and dorsal muscle(3.8 to 5.4 fold)of largemouth bass(P<0.05).In addition,compared to the C0 group,the liver vacuolation area of fish was significantly increased in the C75 and C100 groups(P<0.05).Transcriptional levels of apoptosis-related genes of b-cell lymphoma-2(bcl2),caspase-9-like(casp9),and caspase-3a(casp3)were markedly upregulated(0.9 to 1.6 fold)in the C100 group compared to the C0 group(P<0.05).Based on the quadratic regression analysis between FBW,WGR,or SGR and dietary Chlorella meal level,largemouth bass had the best growth when replacing 31.7%to 32.6%of fish meal with 15.03%to 15.43%dietary Chlorella meal.The present results indicated that dietary supplementation with Chlorella meal(11.85%to 47.45%)significantly enhanced the pigmentation;however,total replacement of fish meal(40%)with Chlorella meal(47.45%)caused growth retardation,apoptosis,and liver damage in largemouth bass. | Longwei Xi Qisheng Lu Yulong Liu Jingzhi Su Wen Chen Yulong Gong Dong Han Yunxia Yang Zhimin Zhang Junyan Jin Haokun Liu Xiaoming Zhu Shouqi Xie | 2022 | Animal Nutrition2022，，3： | 2 |
| 5 | Fanconi anemia gene-associated germline predisposition in aplastic anemia and hematologic malignancies显示文摘Whether Fanconi anemia(FA)heterozygotes are predisposed to bone marrow failure and hematologic neoplasm is a crucial but unsettled issue in cancer prevention and family consulting.We retrospectively analyzed rare possibly significant variations(PSVs)in the five most obligated FA genes,BRCA2,FANCA,FANCC,FANCD2,and FANCG,in 788 patients with aplastic anemia(AA)and hematologic malignancy.Sixty-eight variants were identified in 66 patients(8.38%).FANCA was the most frequently mutated gene(n=29),followed by BRCA2(n=20).Compared with that of the ExAC East Asian dataset,the overall frequency of rare PSVs was higher in our cohort(P=0.016).BRCA2 PSVs showed higher frequency in acute lymphocytic leukemia(P=0.038),and FANCA PSVs were significantly enriched in AA and AML subgroups(P=0.020;P=0.008).FA-PSV-positive MDS/AML patients had a higher tumor mutation burden,higher rate of cytogenetic abnormalities,less epigenetic regulation,and fewer spliceosome gene mutations than those of FA-PSV-negative MDS/AML patients(P=0.024,P=0.029,P=0.024,and P=0.013).The overall PSV enrichment in our cohort suggests that heterozygous mutations of FA genes contribute to hematopoietic failure and leukemogenesis. | Daijing Nie Jing Zhang Fang Wang Xvxin Li Lili Liu Wei Zhang Panxiang Cao Xue Chen Yang Zhang Jiaqi Chen Xiaoli Ma Xiaosu Zhou Qisheng Wu Ming Liu Mingyue Liu Wenjun Tian Hongxing Liu | 2022 | Frontiers of Medicine2022，16，3： | 1 |
| 6 | The Analyses to Some Issues in the Polymerization Level Entity Simulation 显示文摘 | Yang Ligong Guo Qisheng | 2006 | The Journal of System Simulation2006，18，2： | 1 |
| 7 | Characterizing the spatiotemporal variations of evapotranspiration and aridity index in mid-western China from 2001 to 2016显示文摘Mid-western China is one of the most sensitive and fragile areas on the Earth.Evapotranspiration(ET)is a key part of hydrological cycle in these areas and is affected by both global climate change and human activities.The dynamic changes in ET and potential evapotranspiration(PET),which can reflect water consumption and demand,are still unclear,and there is a lack of predictive capacity on drought severity.In this study,we used global MODIS(moderate-resolution imaging spectroradiometer)terrestrial ET(MOD16)products,Morlet wavelet analysis,and simple linear regression to investigate the spatiotemporal variations of ET,PET,reference ET(ET0),and aridity index(AI)in mid-western pastoral regions of China(including Gansu Province,Qinghai Province,Ningxia Hui Autonomous Region,and part of Inner Mongolia Autonomous Region)from 2001 to 2016.The results showed that the overall ET gradually increased from east to southwest in the study area.Actual ET showed an increasing trend,whereas PET tended to decrease from 2001 to 2016.The change in ET was affected by vegetation types.During the study period,the average annual ET0 and AI tended to decrease.At the monthly scale within a year,AI value decreased from January to July and then increased.The interannual variations of ET0 and AI showed periodicity with a main period of 14 a,and two other periodicities of 11 and 5 a.This study showed that in recent years,drought in these pastoral regions of mid-western China has been alleviated.Therefore,it is foreseeable that the demand for irrigation water for agricultural production in these regions will decrease. | MU Le LU Yixiao LIU Minguo YANG Huimin FENG Qisheng | 2021 | Journal of Arid Land2021，13，12： | 1 |
| 8 | Theoreti- cal model and novel numerical approach of a broadband optically pumped three-level alkali vapour laser 显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | J Phys B : At Mol Opt Phys2011，44，08： | 1 |
| 9 | Brandley F Chmelka and Galen D Stucky,Topological construction of mesoporous materials显示文摘 | Dongyuan Zhao Peidong Yang Qisheng Huo | 1998 | Crrent Opinion in Solid State & Materials Science1998，，3： | 1 |
| 10 | Theoretical model and novel numerical approach of a broadband optically pumped three- level alkali vapor laser显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | J Phys B: At Mol Opt Phys2011，44，08： | 1 |
| 11 | Theoretical model and novel numerical approach of a broadband optically pumped three- level alkali vapour laser显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | J Phys B: At Mol Opt Phys2011，44，08： | 1 |
| 12 | Modeling, numerical approach, and power scaling of alkali vapor lasers in side-pumped configuration with flowing medium显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | JOpt SocArn B2011，28，6： | 1 |
| 13 | Luminous Butterflies: Efficient Exciton Harvesting by Benzophenone Derivatives for Full‐Color Delayed Fluorescence OLEDs显示文摘 | Sae Youn Lee Takuma Yasuda Yu Seok Yang Qisheng Zhang Chihaya Adachi | 2014 | Angew. Chem. Int. Ed2014，，25： | 1 |
| 14 | Theoretical model and novel numerical approach of a broadband optically pumped three- level alkali vapor laser显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | Journal of Physics B : Atomic Molecular and Optical Physics2011，44，08： | 1 |
| 15 | Modeling, numerical approach, and power scaling of alkali vapor lasers in side-pumped configuration with flowing medium显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | Opt Soc Am B2011，28，6： | 1 |
| 16 | Theoretical model and novel numerical approach of a broadband optically pumped threelevel alkali vapour laser显示文摘 | Yang Zining Wang Hongyan Lu Qisheng | 2011 | J Phys B: At Mol Opt Phys2011，44，08： | 1 |
| 17 | Tuning the Excited State of Tetradentate Pd(ll) and Pt(ll) Complexes through Benzannulated N-Heteroaromatic Ring and Central Metal显示文摘A series of tetra de ntate Pd(ll)and Pt(ll)complexes containing fused 5/6/6 metallocycles with phe nyl/V-heteroaromatic ben-zo[d]imidazole(pbiz),benzo[d]oxazole(pboz)or benzo[d]thiazole(pbthz)-containing ligands was developed.Systematic studies by experiments and theoret:ical calculations reveal that both the central metal and the benzannulated A/-heteroaromatic ring have sig-n ificant in flue nee on the electrochemical,photophysical and excited-state properties of the Pd(ll)and Pt(ll)complexes.In identical condition,compared to pb/z-based Pd(ll)and Pt(ll)complexes,the corresponding metal complexes with pboz and pbthz-containing ligand show significant red-shift emission spectra. | Guijie Hua Guo Xiaoli Fang Yun-Fang Yang Yulu Sun Weiwei Lou Qisheng Zhang Yuanbin She | 2022 | Chinese Journal of Chemistry2022，40，2： | 0 |
| 18 | Structure of SARS-CoV-2 main protease in the apo state显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)has been a global pandemic that severely threatens global health with concordant economic damage.However,there is currently no clinically approved vaccines or drugs against COVID-19(Lu et al.,2020). | Xuelan Zhou Fanglin Zhong Cheng Lin Xiaohui Hu Yan Zhang Bing Xiong Xiushan Yin Jinheng Fu Wei He Jingjing Duan Yang Fu Huan Zhou Peter J.Mc Cormick Qisheng Wang Jian Li Jin Zhang | 2021 | Science China(Life Sciences)2021，64，4： | 0 |
| 19 | Effect of Pennisetum purpureum Schum cv. Guiminyin on Growth Performance of Geese显示文摘To explore the feeding value of Pennisetum purpureum Schum cv. Guiminyin in production of geese,effects of feeding the green fodder on growth performance of geese were studied,while P. purpureum K. Scbumacb × P. typhoideum Rich cv. Reyan No. 4 was used as control. The results showed that P. purpureum Schum cv. Guiminyin had high biomass and good nutritional quality,and geese intake of the forage was greater than that of the control variety. The average daily gain was(51. 45 ± 3. 49) g/goose,which was increased by 13. 36% compared with control; feed gain ratio was(4. 93 ± 0. 35) %,and feed consumption was reduced by 19. 47% compared with control( P < 0. 05). Thus,it could be concluded that feeding geese with P. purpureum Schum cv. Guiminyin could obtain good economic benefit. | Yao Na Lai Zhiqiang Shi Jing Yang Qisheng Zeng Fanquan | 2016 | Animal Husbandry and Feed Science2016，8，6： | 0 |
| 20 | Application Advantages of Fan-Water Curtain Cooling System in Pig Production显示文摘[Objectives]The purpose was to solve the problem that high temperature and high humidity will seriously affect the health and weight gain of pigs.[Methods]The fan-water curtain cooling system was popularized in large-scale pig farms.[Results]The pigs using the water curtain cooling system gained 1.5 kg from weaning to the day of slaughter.The feed-to-gain ratio was 2.43∶1,a year-on-year decrease of 0.05.The average weight gain per pig was about 5-10 kg.[Conclusions]The application of fan-water curtain cooling system in pig production can accelerate the growth rate of pigs,reduce the occurrence of diseases and improve the utilization of feed,eventually improving the productivity of pigs. | Taiyin LI Jiarong WU Kejun LIU Hongying DENG Zhengyu CHEN Qisheng YANG Chunhua HUANG | 2020 | Asian Agricultural Research2020，12，6： | 0 |
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