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| 1 | Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L显示文摘Clubroot disease,a major plant root disease caused by Plasmodiophora brassicae,has become one of the most destructive diseases among cultivated cruciferous vegetables.However,clubroot-resistant Brassica oleracea materials are rare.A few clubroot-resistant cabbage varieties are available on the market,but all are Ogura cytoplasmic male sterile(CMS)types.Therefore,in this study,to reutilize the clubroot-resistant Ogura CMS germplasm of cabbage,a new fertility-restored Ogura CMS material,16Q2-11,was used as a bridge to transfer the clubroot resistance(CR)gene from the Ogura CMS cytoplasm to the normal cytoplasm by a two-step method(a fertility restoration and cytoplasm replacement method).In the first cross for fertility restoration of Ogura CMS clubroot-resistant cabbage(FRCRC),16Q2-11 was used as a restorer to cross with Ogura CMS materials containing the CR gene CRb2.Eleven Rfo-positive progenies were generated,of which four contained CRb2:F8-514,F8-620,F8-732 and F8-839.After inoculation with race 4 of P.brassicae,these four CRb2-positive individuals showed resistance.Furthermore,F8-514 and F8-839 were then used as male parents in the second cross of FRCRC to cross with cabbage inbred lines,resulting in the successful introgression of the CRb2 gene into the inbred lines.All offspring produced from this step of cross,which had a normal cytoplasm,showed a high resistance to race 4 of P.brassicae and could be utilized for the breeding of clubrootresistant cabbage varieties in the future.This is the first time that the Ogura CMS restorer has been used to restore the fertility of Ogura CMS clubroot-resistant cabbages,which could improve germplasm diversity in cabbage and provide a reference method for using CMS germplasm in Brassica crops. | Wenjing Ren Zhiyuan Li Fengqing Han Bin Zhang Xing Li Zhiyuan Fang Limei Yang Mu Zhuang Honghao Lv Yumei Liu Yong Wang Hailong Yu Yangyong Zhang | 2020 | Horticulture Research2020，7，1： | 7 |
| 2 | An update on the arsenal:mining resistance genes for disease management of Brassica crops in the genomic era显示文摘Brassica species include many economically important crops that provide nutrition and health-promoting substances to humans worldwide.However,as with all crops,their production is constantly threatened by emerging viral,bacterial,and fungal diseases,whose incidence has increased in recent years.Traditional methods of control are often costly,present limited effectiveness,and cause environmental damage;instead,the ideal approach is to mine and utilize the resistance genes of the Brassica crop hosts themselves.Fortunately,the development of genomics,molecular genetics,and biological techniques enables us to rapidly discover and apply resistance(R)genes.Herein,the R genes identified in Brassica crops are summarized,including their mapping and cloning,possible molecular mechanisms,and application in resistance breeding.Future perspectives concerning how to accurately discover additional R gene resources and efficiently utilize these genes in the genomic era are also discussed. | Honghao Lv Zhiyuan Fang Limei Yang Yangyong Zhang Yong Wang | 2020 | Horticulture Research2020，7，1： | 2 |
| 3 | Transparent conductive thin films based on polyaniline nanofibers 显示文摘 | Wang Yangyong Jing Xinli | 2007 | Materials Science and Engineering B2007，138，： | 1 |
| 4 | Sonochemical synthesis of polyaniline nanofibers 显示文摘 | Jing Xinli Wang Yangyong Wu Dan | 2007 | Ultrasonics Sono- chemistry2007，14，1： | 1 |
| 5 | Polyaniline nanofibers prepared with ultrasonic irradiation 显示文摘 | Jing Xinli Wang Yangyong Wu Dan | 2006 | Journal of Polymer Science Part A: Polymer Chemistry2006，44，2： | 1 |
| 6 | Effects of ultrason- ic irradiation on the morphology of chemically prepared poly- aniline nanofibers显示文摘 | Li Yu Wang Yangyong Wu Dan | 2009 | Journal of Applied Polymer Science2009，113，2： | 1 |
| 7 | 显示文摘 | Xinli Jing Yangyong Wang Dan Wu | 2006 | J Polym Sci Pol Chem2006，44，： | 1 |
| 8 | 显示文摘 | Xinli Jing Yangyong Wang Dan Wu Jipeng Qiang | 2007 | Ultrason Sonochem2007，14，： | 1 |
| 9 | Polyaniline nanofibers prepared with hydrogen peroxide as oxidant显示文摘 | Yangyong Wang Xinli Jing Junhua Kong | 2007 | Synthetic Metals2007，，6： | 1 |
| 10 | Set-valued information sys-tems显示文摘 | Guan Yangyong Wang Hongkai | 2006 | Information Sciences2006，176，17： | 1 |
| 11 | 显示文摘 | Yangyong Wang Xinli Jing | 2008 | J Phys Chem B2008，112，： | 1 |
| 12 | 显示文摘 | Wang Yangyong Jing Xinli Kong Junhua | 2007 | Synthetic Met2007，157，： | 1 |
| 13 | Polyaniline nanofibers prepared with ultrasonic irradiation 显示文摘 | Jing Xinli Wang Yangyong Wu Dan | 2006 | Jour- nal of Polymer Science: Part A: Polymer Chemistry2006，44，： | 1 |
| 14 | Synthesis and applications of one-dimensional nano-structured polyaniline: An overview 显示文摘 | Zhang Donghua Wang Yangyong | 2006 | Materials Science and Engineering B2006，134，1： | 1 |
| 15 | Measurement and encoder based on neural networks Technology, 2013 a calibration method for an optical daptive differential evolution-Fourier 显示文摘 | Deng Fang Chen Jie Wang Yangyong | 2013 | Measurement Science and2013，24，5： | 1 |
| 16 | Dissection of two QTL clusters underlying yield-related heterosis in the cabbage founder parent 01–20显示文摘Cabbage has significant heterosis and most commercial cultivars are hybrids.To explore genetic basis of cabbage heterosis and promote cabbage heterosis utilization,we constructed two populations by crossing 100 DH lines derived from a cabbage hybrid 01–20×96–100 with two female parents.Hybrids exhibited different extents of heterosis,the mean value of economic yield was 2.6 times bigger than parents.We identified 66 and 73 QTLs associated with mid-parent heterosis and transgressive heterosis of twelve yield-related traits,respectively.Some QTLs could be detected under the two-year experiment existed in two populations with different testers,showing relatively high phenotypic contribution rate(15.8%–20.0%).Heterosis QTLs exhibited clustered distribution in several cabbage chromosome regions.Two dominant genetic regions,mk300–316 and mk258–268,originated from the elite parent 01–20,exhibited significant genetic effects for yield-related heterosis,which were first identified.Three elite DH lines(D22,D46,D83)harboring these two dominant regions were selected as having strong heterosis in cabbage production.Candidate gene analysis revealed that some genes participating in biosynthetic processes of carbohydrates and some responses to auxin might affect cabbage yield heterosis.QTL identification and genetic dissection of yield-related traits provide new insights into the genetic effects of cabbage heterosis. | Xing Li Honghao Lv Bin Zhang Zhiyuan Fang Limei Yang Mu Zhuang Yumei Liu Zhansheng Li Yong Wang Yangyong Zhang | 2023 | Horticultural Plant Journal2023，9，1： | 1 |
| 17 | Transparent conductive thin films based on polyaniline nanofibers显示文摘 | Yangyong Wang Xinli Jing | 2007 | Materials Science and Engineering B2007，，138： | 1 |
| 18 | 显示文摘 | Jing Xinli Wang Yangyong Zhang Baiyu | 2005 | Journal of Applied Polymer Science2005，98，： | 1 |
| 19 | Stable epidermal electronic device with strain isolation induced by in situ Joule heating显示文摘Epidermal electronics play increasingly important roles in human-machine intetfaces.However,their efficient fabrication while maintaining device stability and reliability remains an unresolved challenge.Here,a facile in situ Joule heating method is proposed for fabricating stable epidermal electronics on a polyvinyl alcohol(PVA)substrate.Benefiting from the precise control of heating locations,the crystallization and enhanced rigidity of PVA are restricted to desired areas,leading to strain isolation of the active regions.As a result,the electronic device can be conformably attached to skin while showing negligible degradation in device performance during deformation.Based on this method,a flexible surface electromyography(sEMG)sensor with outstanding stability and highly comfortable wearability is demonstrated,showing high accuracy(91.83%)for human hand gesture recognition.These results imply that the fabrication method proposed in this research is a facile and reliable approach for the fabrication of epidermal electronics. | Zihao Wang Qifeng Lu Yizhang Xia Simin Feng Yixiang Shi Shuqi Wang Xianqing Yang Yangyong Zhao Fuqin Sun Tie Li Ting Zhang | 2021 | Microsystems & Nanoengineering2021，7，4： | 0 |
| 20 | Map-based cloning and CRISPR/Cas9-based editing uncover BoNA1 as the causal gene for the no-anthocyanin-accumulation phenotype in curly kale (Brassica oleracea var. sabellica)显示文摘Brassica oleracea comprises several important vegetable and ornamental crops,including curly kale,ornamental kale,cabbage,broccoli,and others.The accumulation of anthocyanins,important secondary metabolites valuable to human health,in these plants varies widely and is responsible for their pink to dark purple colors.Some curly kale varieties lack anthocyanins,making these plants completely green.The genetic basis of this trait is still unknown.We crossed the curly kale inbred line BK2019(without anthocyanins)with the cabbage inbred line YL1(with anthocyanins)and the Chinese kale inbred line TO1000(with anthocyanins)to generate segregating populations.The no-anthocyanin trait was genetically controlled by a recessive gene,bona1.We generated a linkage map and mapped bona1 to a 256-kb interval on C09.We identified one candidate gene,Bo9g058630,in the target genomic region;this gene is homologous to AT5G42800,which encodes a dihydroflavonol-4-reductase-like(DFR-like)protein in Arabidopsis.In BK2019,a 1-bp insertion was observed in the second exon of Bo9g058630 and directly produced a stop codon.To verify the candidate gene function,CRISPR/Cas9 gene editing technology was applied to knock out Bo9g058630.We generated three bona1 mutants,two of which were completely green with no anthocyanins,confirming that Bo9g058630 corresponds to BoNA1.Different insertion/deletion mutations in BoNA1 exons were found in all six of the other no-anthocyanin kale varieties examined,supporting that independent disruption of BoNA1 resulted in no-anthocyanin varieties of B.oleracea.This study improves the understanding of the regulation mechanism of anthocyanin accumulation in B.oleracea subspecies. | Kaiwen Yuan Xinyu Zhao Wenru Sun Limei Yang Yangyong Zhang Yong Wang Jialei Ji Fengqing Han Zhiyuan Fang Honghao Lv | 2023 | Horticulture Research2023，10，8： | 0 |
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