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| 1 | A Chromosome-Scale Genome Assembly of Paper Mulberry(Bmussonetia papyrifera)Provides New Insights into Its Forage and Papermaking Usage显示文摘Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide. | Xianjun Peng Hui Liu Peilin Chen Feng Tang Yanmin Hu Fenfen Wang Zhi Pi Meiling Zhao Naizhi Chen Hui Chen Xiaokang Zhang Xueqing Yan Min Liu Xiaojun Fu Guofeng Zhao Pu Yao Lili Wang He Dai Xuming Li Wei Xiong Wencai Xu Hongkun Zheng Haiyan Yu Shihua Shen | 2019 | Molecular Plant2019，12，5： | 18 |
| 2 | Characteristics of gas-solid micro fluidized beds for thermochemical reaction analysis显示文摘Fuel conversion and clean energy reaction systems involve a variety of catalytic and non-catalytic gas-solid thermochemical reactions.A good understanding of the correct reaction mechanism and kinetics,as well as the profiles of reaction products,is of great significance to the development,design,and operation of such reaction systems.The micro fluidized bed reaction analysis provides an efficient and reliable method to acquire this essential information with low capital and operating costs,low energy consumption and enhanced safety.This paper provides an overview of the system and its characteristics for the micro fluidized bed reaction analyzer that has been well proven to be a reliable new approach as well as an instrument for characterizing various gas-solid thermochemical reactions. | Zhennan Han Junrong Yue Xi Zeng Jian Yu Fang Wang Shaozeng Sun Hong Yao Guangqian Luo Xuejing Liu Yining Sun Fu Ding Liangliang Fu Lei Shi Kangjun Wang Jiebin Yang Shaonan Wang Xueqing Chen Dingrong Bai Guangwen Xu | 2020 | Carbon Resources Conversion2020，3，1： | 6 |
| 3 | Modified Shenlingbaizhu decoction reduces intestinal adenoma formation in adenomatous polyposis coli multiple intestinal neoplasia mice by suppression of hypoxia-inducible factor 1α-induced CD4+CD25+forkhead box P3 regulatory T cells显示文摘OBJECTIVE: To test the hypothesis that modified Shenlingbaizhu decoction(MSD) attenuates the formation of intestinal adenomas by regulating activation of CD4+CD25+ forkhead box P3(Fox P3) regulatory T cells(Tregs) by downregulation of hypoxia-inducible factor 1α(HIF-1α).METHODS: Chemical fingerprints of ginsenoside Rb1, ginsenoside Rc, paeoniflorin, and dioscin in standard extractions were used as material bases of MSD. Adenomatous polyposis coli multiple intestinal neoplasia(ApcMin/+) mice, which harbor a mutation in adenomatous polyposis coli, were used to host intestinal adenomas. Peripheral blood and spleen Tregs were analyzed by flow cytometry. Protein expression was analyzed by immunohistochemistry and Western blotting.RESULTS: The number and size of intestinal adenomas were significantly reduced by MSD treatment.Mucosal thickening and the spleen size were also substantially decreased by MSD. The carcinogenesis process in ApcMin/+mice resembled that of human colorectal cancer. Molecular markers of neoplasms, such as β-catenin, cyclooxygenase-2, proliferating cell nuclear antigen, and p53, were substan-tially ameliorated by MSD treatment. Moreover,MSD downregulated peripheral and spleen CD4 +CD25+Fox P3+ Tregs and reduced in situ expression of CD4, CD25, and Fox P3 in intestinal adenomas.MSD also suppressed HIF-1α expression in the intestinal adenomas, and HIF-1α inhibition decreased expression of Fox P3 in Jurkat T cells under hypoxic conditions.CONCLUSION: MSD is a valid prescription to control the formation of intestinal adenomas in ApcMin/+mice. It exerts anti-cancer effects partially through suppression of HIF-1α that induced activation of CD4+CD25+Fox P3+ Tregs in vivo and in vitro. | Xu Wenjuan Han Qinrui Liang Shuntian Li Lu Shao Meng Yao Xueqing Sun Xuegang | 2018 | Journal of Traditional Chinese Medicine2018，38，1： | 3 |
| 4 | AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua显示文摘Artemisia annua,a traditional Chinese medicinal plant,remains the only plant source for artemisinin production,yet few genes have been identified to be involved in both the response to biotic stresses,such as pathogens,and artemisinin biosynthesis.Here,we isolated and identified the WRKY transcription factor(TF)AaWRKY17,which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A.annua.Yeast one-hybrid(Y1H),dual-luciferase(dual-LUC),and electrophoretic mobility shift assay(EMSA)results showed that AaWRKY17 directly bound to the W-box motifs in the promoter region of the artemisinin biosynthetic pathway gene amorpha-4,11-diene synthase(ADS)and promoted its expression.Real-time quantitative PCR(RT-qPCR)analysis revealed that the transcript levels of two defense marker genes,Pathogenesis-Related 5(PR5)and NDR1/HIN1-LIKE 10(NHL10),were greatly increased in AaWRKY17-overexpressing transgenic A.annua plants.Additionally,overexpression of AaWRKY17 in A.annua resulted in decreased susceptibility to P.syringae.These results indicated that AaWRKY17 acted as a positive regulator in response to P.syringae infection.Together,our findings demonstrated that the novel WRKY transcription factor AaWRKY17 could potentially be used in transgenic breeding to improve the content of artemisinin and pathogen tolerance in A.annua. | Tiantian Chen Yongpeng Li Lihui Xie Xiaolong Hao Hang Liu Wei Qin Chen Wang Xin Yan Kuanyu Wu-Zhang Xinghao Yao Bowen Peng Yaojie Zhang Xueqing Fu Ling Li Kexuan Tang | 2021 | Horticulture Research2021，8，1： | 2 |
| 5 | High-energy-density plasma in femtosecond-laser-irradiated nanowire-array targets for nuclear reactions显示文摘In this work,the high-energy-density plasmas(HEDP)evolved from joule-class-femtosecond-laser-irradiated nanowire-array(NWA)targets were numerically and experimentally studied.The results of particle-in-cell simulations indicate that ions accelerated in the sheath field around the surfaces of the nanowires are eventually confined in a plasma,contributing most to the high energy densities.The protons emitted from the front surfaces of the NWA targets provide rich information about the interactions that occur.We give the electron and ion energy densities for broad target parameter ranges.The ion energy densities from NWA targets were found to be an order of magnitude higher than those from planar targets,and the volume of the HEDP was several-fold greater.At optimal target parameters,8%of the laser energy can be converted to confined protons,and this results in ion energy densities at the GJ/cm^(3) level.In the experiments,the measured energy of the emitted protons reached 4 MeV,and the changes in energy with the NWA’s parameters were found to fit the simulation results well.Experimental measurements of neutrons from 2H(d,n)3He fusion with a yield of(24±18)×10^(6)/J from deuterated polyethylene NWA targets also confirmed these results. | Defeng Kong Guoqiang Zhang Yinren Shou Shirui Xu Zhusong Mei Zhengxuan Cao Zhuo Pan Pengjie Wang Guijun Qi Yao Lou Zhiguo Ma Haoyang Lan Wenzhao Wang Yunhui Li Peter Rubovic Martin Veselsky Aldo Bonasera Jiarui Zhao Yixing Geng Yanying Zhao Changbo Fu Wen Luo Yugang Ma Xueqing Yan Wenjun Ma | 2022 | Matter and Radiation at Extremes2022，7，6： | 1 |
| 6 | Small-angle X-ray scattering study on the orientation of suspended sodium titanate nanofiber induced by applied electric field显示文摘Introduction Nanofiber orientation in suspensions determines the performance of nanoparticle suspensions which have potential applications in intelligent control.Material and method Na2Ti3O7 nanofibers were prepared with a hydrothermal method.The orientation degree of Na2Ti3O7 nanofibers in silicone oil has been studied by in situ small-angle X-ray scattering technique.Thünemann–Ruland method was used to extract the distribution widths of Na2Ti3O7 nanofibers in the suspensions.Conclution An empirical formula has been proposed to describe the dependence of nanofiber orientation degree on the external electric-field strength(E)and the nanofiber concentration(C).The results demonstrate that the response of nanofiber orientation to the electric field can be divided into exponential and linear stages before and after the inflection point of electric-field strength(Ec0.09 kV/mm).Low concentration of suspension is more sensitive to the external electric field.The increase in nanofiber concentration will decrease the response sensitivity of nanofiber orientation degree to the change of E.The critical concentration of Na2Ti3O7 nanofibers in the suspension is about 5 wt%.This study is expected to give new clue for the structurally responsive mechanism of anisotropic nanoparticles in suspensions to electric-field strength and particle concentration. | Jiayi Wang Xiaoyi Zhao Yunpeng Liu Lixiong Qian Lei Yao Xueqing Xing Guang Mo Quan Cai Zhongjun Chen Zhonghua Wu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 7 | Obesity-induced inflammatory miR-133a mediates apoptosis of granulosa cells and causes abnormal folliculogenesis显示文摘Obesity has been reported to promote disordered folliculogenesis,but the exact molecular mechanisms are still not fully understood.In this study,we find that miR-133a is involved in obesity-induced follicular development disorder.After feeding with a high-fat diet(HFD)and fructose water for nine weeks,the mouse body weight is significantly increased,accompanied by an inflammatory state and increased expression of miR-133a in the adipose tissues and ovaries as well as accelerated follicle depletion.Although miR-133a is increased in the fat and ovaries of HFD mice,the increased miR-133a in the HFD ovaries is not derived from exosome transferred from obese adipose tissues but is synthesized by ovarian follicular cells in response to HFD-induced inflammation.In vivo experiments show that intrabursal injection of miR-133a agomir induces a decrease in primordial follicles and an increase in antral follicles and atretic follicles,which is similar to HFD-induced abnormal folliculogenesis.Overexpression of miR-133a modestly promotes granulosa cell apoptosis by balancing the expression of anti-apoptotic proteins such as C1QL1 and XIAP and pro-apoptotic proteins such as PTEN.Overall,this study reveals the function of miR-133a in obesityinduced ovarian folliculogenesis dysfunction and sheds light on the etiology of female reproductive disorders. | Ruizhi Chen Xueqing Wu Han Qiu Baiming Yang Yao Chen Xiang Chen Yingshan Li Shaochun Yuan Dan Liu Luanjuan Xiao Yanhong Yu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
| 8 | A novel SAXS/XRD/XAFS combined technique for in-situ timeresolved simultaneous measurements显示文摘Synchrotron radiation based combined technique can provide multiple structural information simultaneously,which is an important development direction of structural detection.In this study,a novel small-angle X-ray scattering/X-ray diffraction/X-ray absorption fine structure(SAXS/XRD/XAFS)combined setup was constructed,where an area detector,a curved detector,and a point detector are,respectively,used for the measurements of SAXS,XRD,and XAFS signals.A detailed description about the combined setup was given.A minitype diamond detector coupled to a SAXS beamstop was used to record the transmitted X-ray intensity,making the scattering(SAXS and XRD)signal measurement compatible with the absorption(XAFS)signal measurement,avoiding mechanical switching.The two-way sampling strategy was used to acquire XAFS signals,shortening the non-counting time.The two-way and one-way sampling strategies were discussed.High-frequency sampling scheme was used to collect experimental signals,improving the measurement efficiency and signal-to-noise ratio.A detailed description and discussion about the high-frequency scheme were also given in this paper.Except the rotation of monochromator,there is no mechanical movement in measurements,time resolution may reach the level of seconds.Using this SAXS/XRD/XAFS combined setup,SAXS,XRD,and XAFS signals can be acquired simultaneously.With some in-situ sample environment system,the newlydeveloped combined technique can be used to track the structure evolution in complex fluids.During the formation processes of(BiO)2CO3 and ZnAPO-34 particles,the changes of in-situ experimental data with reaction time demonstrate that SAXS/XRD/XAFS combined technique is feasible to track the dynamic process. | Zhonghua Wu Yunpeng Liu Xueqing Xing Lei Yao Zhongjun Chen Guang Mo Lirong Zheng Quan Cai Hao Wang Jiajun Zhong Yuecheng Lai Lixiong Qian | 2023 | Nano Research2023，16，1： | 0 |
| 9 | 6-2 Recent Progress of the SSC-Linac显示文摘SSC-Linac[1] as the injector for the Separated Sector Cyclotron (SSC), is being constructed at national labo-ratory Heavy Ion Research Facility at Lanzhou (HIRFL). The injection and extraction energy are 3.7 keV/u and1.025 MeV/u, respectively. Fig. 1 shows the front end section of the SSC-Linac. The high charges state continuswave (CW) 4-rod RFQ has been designed and fabricated for some years. From the end of 2013, the commissioningof the RFQ was performed and the beam test was carried out in April 2014. In this paper the experiment resultwill be presented. | Yin Xuejun Yuan Youjin Zhang Xiaohu Lu Wang Du Heng Li Xiaoni Jiang Peiyong Li Zhongcan Wang Haonin Yang Yaqing Ma Lizhen Xu Zhe Zhang Xuezhen Sun Liangting Yao Qinggao Meng Jun Cai Guozhu He Yuan Zhao Hongwei Xia Jiawen Liu Ge Lu Yuanrong Zhu Kun Gao Shuli Yan Xueqing Chen Jiaer | 2014 | IMP & HIRFL Annual Report2014，，1： | 0 |
| 10 | Efficient electrocatalytic CO_(2)reduction to C_(2+)chemicals on internal porous copper显示文摘To improve the electrocatalytic conversion of carbon dioxide(CO_(2))into C_(2+)products(such as ethylene(C_(2)H_(4))and ethanol(CH_(3)CH_(2)OH),etc.)is of great importance,but remains challenging.Herein,we proposed a strategy that directs the C–C coupling pathway through enriching and confining the carbon monoxide(CO)intermediate to internal pores of Cu nanocubes,for electrocatalytic reduction of CO_(2)into C_(2+)chemicals.In H-type cell,the Faraday efficiency(FE)for ethylene and ethanol reaches 70.3%at−1.28 V versus the reversible hydrogen electrode(vs.RHE),with a current density of 47.9 mA·cm^(−2).In flow cell,the total current density is up to 340.3 mA·cm^(−2)at^(−2).38 V(vs.RHE)and the FE for C_(2+)products is 67.4%.Experimental and theoretical studies reveal that both the CO intermediate adsorption and C–C coupling reaction on such an internal porous catalyst are facilitated,thus improving CO_(2)-to-C_(2+)conversion efficiency. | Sha Wang Jianling Zhang Lei Yao Yisen Yang Lirong Zheng Bo Guan Yingzhe Zhao Yanyue Wang Buxing Han Xueqing Xing | 2023 | Nano Research2023，16，8： | 0 |
| 11 | High-resolution aging niche of human adipose tissues显示文摘Dear Editor,Age-dependent adipose tissue malfunction raises the risk of diseases like diabetes,cardiovascular disease,and even cancer by contributing to metabolic decline,heterotopic fat storage,and chronic systemic inflammation.1 Understanding adipose tissue aging requires in-depth knowledge of the cellular and molecular properties of various adipose tissue cell types.Although the heterogeneity of the cell population during mouse aging has been studied,2 little is known about the cellular and molecular basis of human adipose tissues aging. | Wenyan Zhou Junxin Lin Yan Xie Xueqing Hu Xudong Yao Yuemin Ou Hongwei Wu Yiyang Yan Xiumao Li Jianan Lou Aaron Trent Irving James Q.Wang Hongwei Ouyang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 0 |
| 12 | Pterostilbene accelerates wound healing by modulating diabetes-induced estrogen receptorβsuppression in hematopoietic stem cells显示文摘Background:Delayed wound healing is one of the major complications of diabetes mellitus and is characterized by prolonged inflammation,delayed re-epithelialization and consistent oxidative stress,although the detailed mechanism remains unknown.In this study,we aimed to investigate the potential role and effect of pterostilbene(PTE)and hematopoietic stem cells(HSCs)on diabetic wound healing.Methods:Diabetic rats were used to measure the epigenetic changes in both HSCs and peripheral blood mononuclear cells(PBMCs).A cutaneous burn injury was induced in the rats and PTE-treated diabetic HSCs were transplanted for evaluation of wound healing.In addition,several biomedical parameters,including gene expression,oxidative stress,mitochondrial function and inflammation in macrophages,were also measured.Results:Our data showed that PTE had a much stronger effect than resveratrol on accelerating diabetic wound healing,likely because PTE can ameliorate diabetes-induced epigenetic changes to estrogen receptorβpromoter in HSCs,while resveratrol cannot.Further investigation showed that bone marrow transplantation of PTE-treated diabetic HSCs restores diabetes-induced suppression of estrogen receptorβand its target genes,including nuclear respiratory factor-1 and superoxide dismutase 2,and protects against diabetes-induced oxidative stress,mitochondrial dysfunction and elevated pro-inflammatory cytokines in both PBMCs and macrophages,subsequently accelerating cutaneous wound healing.Conclusions:HSC may play an important role in wound healing through transferring epigenetic modifications to subsequent PBMCs and macrophages by differentiation,while PTE accelerates diabetic wound healing by modulating diabetes-induced epigenetic changes in HSCs.Thus,PTE may be a novel therapeutic strategy for diabetic wound healing. | Weiguo Xie Xueqing Zhou Weigang Hu Zhigang Chu Qiongfang Ruan Haimou Zhang Min Li Hongyu Zhang Xiaodong Huang Paul Yao | 2021 | Burns & Trauma2021，9，1： | 0 |
| 13 | One-pot preparation of nanodispersion with readily available components for localized tumor photothermal and photodynamic therapy显示文摘Photothermal(PTT) and photodynamic(PDT) combined therapy has been hindered to clinical translation, due to the lack of available biomaterials, difficult designs of functions,and complex chemical synthetic or preparation procedures. To actualize a high-efficiency combination therapy for cancer via a feasible approach, three readily available materials are simply associated together in one-pot, namely the single-walled carbon nanohorns(SWCNH), zinc phthalocyanine(ZnPc), and surfactant TPGS. The established nanodispersion is recorded as PCT. The association of SWCNH/ZnPc/TPGS was confirmed by energy dispersive spectrum, Raman spectrum and thermogravimetric analysis. Under lighting, PCT induced a temperature rising up to about 60 ℃ due to the presence of SWCNH, production a 7-folds of singlet oxygen level elevation because of ZnPc, which destroyed almost all4T1 tumor cells in vitro. The photothermal effect of PCT depended on both laser intensity and nanodispersion concentration in a linear and nonlinear manner, respectively. After a single peritumoral injection in mice and laser treatment, PCT exhibited the highest tumor temperature rise(to 65 ℃) among all test groups, completely destroyed primary tumor without obvious toxicity, and inhibited distant site tumor. Generally, this study demonstrated the high potential of PCT nanodispersion in tumor combined therapy. | Anpu Yang Yanan Sun Bochen Lyu Binlong Chen Zhipu Fan Minghui Li Yao Zhao Jijun Fu Bing He Hua Zhang Xueqing Wang Wenbing Dai Qiang Zhang | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，1： | 0 |
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