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| 1 | Zebrafish model for assessing induced organ toxicity by Strychnos nux-vomica显示文摘OBJECTIVE:To assess the acute organ toxicity of Strychnos nux-vomica with zebrafish model visually.METHODS:To assess acute toxicity,we initially determined the lethal concentration after Strychnos nux-vomica treatment for 24 h.Zebrafish was treated with five concentrations Strychnos nux-≦vo LC10 for 24 h,and the effects ofmica on morphology,function of heart,central nervous system,liver,kidney and organ-specific cell death were assessed.Next,we assessed the reversibility of toxic effect.RESULTS:Strychnos nux-vomica has an effect on the different organs of zebrafish,including heart,central nervous system,liver,and kidney,and cadiotoxicity induced by Strychnos nux-vomica was reversible to some extent.CONCLUSION:Zebrafish model is suitable for confirming the toxic target organs for Chinese traditional medicine. | Zhao Chongjun Tian Jinghuan Wang Jinfeng Feng Yaru Ni Yuanyuan Fan Jiaojiao Wang Chunmei Cao Dan Zou Qinwen Ma Zhiqiang Lin Ruichao | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 9 |
| 2 | Design,methodology,and baseline of whole city‑million scale children and adolescents myopia survey(CAMS)in Wenzhou,China显示文摘Background:Myopia is the most common visual impairment in children and adolescents worldwide.This study described an economical and effective population-based screening pipeline and performed the project of a million scale children and adolescents myopia survey(CAMS),which will shed light on the further study of myopia from the level of epidemiology and precision medicine.Methods:We developed a novel population-based screening pattern,an intelligent screening process and internetbased information transmission and analysis system to carry out the survey consisting of school children in Wenzhou,China.The examination items include unaided distance visual acuity,presenting distance visual acuity,and noncycloplegic autorefraction.Myopia and high myopia were defined as spherical equivalent(SE)≤−1.00 diopters(D)and SE≤−6.00 D,respectively.Next,the reports of the vision checking were automatically sent to parents and the related departments.The CAMS project will be done two to four times annually with the support of the government.An online eyesight status information management system(OESIMS)was developed to construct comprehensive and efficient electronic vision health records(EVHRs)for myopia information inquiry,risk pre-warning,and further study.Results:The CAMS completed the first-round of screening within 30 days for 99.41%of Wenzhou students from districts and counties,in June 2019.A total of 1,060,925 participants were eligible for CAMS and 1,054,251(99.37%participation rate)were selected through data quality control,which comprised 1305 schools,and 580,609,251,050 and 170,967 elementary,middle,and high school students.The mean age of participants was 12.21±3.32 years(6-20 years),the female-to-male ratio was 0.82.The prevalence of myopia in elementary,middle,and high school students was 38.16%,77.52%,and 84.00%,respectively,and the high myopia incidence was 0.95%,6.90%,and 12.98%.Conclusions:The CAMS standardized myopia screening model involves automating large-scale information collection,data transmission,data analysis and early warning,thereby supporting myopia prevention and control.The entire survey reduced 90%of staff,cost,and time consumption compared with previous surveys.This will provide new insights for decision support for public health intervention. | Liangde Xu Youyuan Zhuang Guosi Zhang Yunlong Ma Jian Yuan Changseng Tu MiaoMiao Li Wencan Wang Yaru Zhang Xiaoyan Lu Jing Li Xinting Liu Zhengbo Xue Meng Zhou Jie Sun Jinhua Bao Ming Li Fan Lu Hong Wang Jianzhong Su Jia Qu | 2021 | Eye and Vision2021，8，1： | 7 |
| 3 | Geological controls of shale gas accumulation and enrichment mechanism in Lower Cambrian Niutitang Formation of western Hubei, Middle Yangtze, China显示文摘The lower Cambrian Niutitang Formation is of crucial importance for shale gas target reservoirs in western Hubei,China;however,little work has been done in this field,and its shale gas accumulation and enrichment mechanism are still unclear.Based on survey wells,outcrop data,and large numbers of tests,the geological conditions of shale gas accumulation were studied;moreover,the factors that influence the gas content were thoroughly discussed.The results show that the Niutitang Formation(Є1n)can be divided into three sections:the first section(Є_(1)n^(1)),the second section(Є_(1)n^(2)),and the third section(Є1n3).TheЄ_(1)n^(2) is the main shale gas reservoir.The deep shelf facies is the main sedimentary facies and can be divided into three main lithofacies:argillaceous siltstone,carbonaceous shale and carbonaceous siliceous rock.The total organic carbon(TOC)content shows gentle growth trends until bottom of theЄ_(1)n^(2) and then decreases rapidly within theЄ_(1)n^(1),and the TOC content mainly ranges from 2%to 4%horizontally.The calcite and dolomite dissolution pores,clay intergranular pores and organic pores are the main pore types and the micropore types are clearly related to the mineral compositions and the TOC content.Vertically,the gas content is mainly affected by the TOC content.Horizontally,wells with high gas contents are distributed only southeast of the Huangling anticline,and the combination of structural styles,fault and fracture development,and the distribution of the regional unconformity boundary between the upper Sinian Dengying Formation(Z2d)and theЄ_(1)n^(2) are the three most important factors affecting the gas content.The favorable areas must meet the following conditions:a deep shelf environment,the presence of theЄ_(1)n^(1),wide and gentle folds,far from large normal faults that are more than 5 km,moderate thermal evolution,and greater than 500 m burial depth;this includes the block with the YD2–ZD2 wells,and the block with the Y1 and YD4 wells,which are distributed in the southern portion of the Huangling anticline and northern portion of the Xiannvshan fault. | Lulu XU Saipeng HUANG Zaoxue LIU Yaru WEN Xianghui ZHOU Yanlin ZHANG Xiongwei LI Deng WANG Fan LUO Cheng CHEN | 2021 | Frontiers of Earth Science2021，15，2： | 3 |
| 4 | OGG1 inhibition suppresses African swine fever virus replication显示文摘African swine fever virus(ASFV)is an important pathogen that causes a highly contagious and lethal disease in swine,for which neither a vaccine nor treatment is available.The DNA repair enzyme 8-oxoguanine DNA glycosylase 1(OGG1),which excises the oxidative base lesion 8-oxo-7,8-dihydroguanine(8-oxoG),has been linked to the pathogenesis of different diseases associated with viral infections.However,the role of OGG1-base excision repair(BER)in ASFV infection has been poorly investigated.Our study aimed to characterize the alteration of host reactive oxygen species(ROS)and OGG1 and to analyse the role of OGG1 in ASFV infection.We found that ASFV infection induced high levels and dynamic changes in ROS and 8-oxoG and consistently increased the expression of OGG1.Viral yield,transcription level,and protein synthesis were reduced in ASFV-infected primary alveolar macrophages(PAMs)treated by TH5487 or SU0268 inhibiting OGG1.The expression of BER pathway associated proteins of ASFV was also suppressed in OGG1-inhibited PAMs.Furthermore,OGG1 was found to negatively regulate interferonβ(IFN-β)production during ASFV infection and IFN-βcould be activated by OGG1 inhibition with TH5487 and SU0268,which blocked OGG1 binding to 8-oxoG.Additionally,the interaction of OGG1 with viral MGF360-14-L protein could disturb IFN-βproduction to further affect ASFV replication.These results suggest that OGG1 plays the crucial role in successful viral infection and OGG1 inhibitors SU0268 or TH5487 could be used as antiviral agents for ASFV infection. | Jie Fan Xinqian Lv Saixia Yang Shuxian Geng Jifei Yang Yaru Zhao Zhonghui Zhang Zhijie Liu Guiquan Guan Jianxun Luo Qiaoying Zeng Hong Yin Qingli Niu | 2023 | Virologica Sinica2023，38，1： | 1 |
| 5 | A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris显示文摘 | Huiying Luo Yaru Wang Hui Wang Jun Yang Yuhui Yang Huoqing Huang Peilong Yang Yingguo Bai Pengjun Shi Yunliu Fan Bin Yao | 2009 | Applied Microbiology and Biotechnology2009，，3： | 1 |
| 6 | Investigation of Linarinic acid and one of its derivatives against cerebral ischemia in mice显示文摘The study aims to investigate the effects of(-)-Linarinic acid(LA) and one of its derivatives(LAd) on brain injury induced by ischemia. Malonaldehyde(MDA) is determined as an index for lipid peroxidation both in vitro and vivo. Mice were pre-treated with LA and LAd for 3 d.Thereafter, they were induced to have incomplete cerebral ischemia with both bilateral carotid artery occlusion and hypotension(BCAOH). In the first part of the in vivo experiment, mice were divided into four groups: sham(control), ischemia, ischemia + LA(200 mg/kg, i.g.) and ischemia + LAd(200 mg/kg, i.g.). In the second part, the dose-response of LAd was investigated at 100, 200 and 400 mg/kg i.g., respectively. A modified neurological severity score was developed for evaluating behavioral deficits of the mice with ischemia. Brains of the mice were excised in order to determinate MDA after ischemia for 6 h. Survival time, survival rate, neurological injury score and MDA level in brains were observed. Results were: 1) The data in vitro showed that both LA and LAd could inhibit the generation of MDA. IC50 values obtained by Probit analysis were 2.9 mM for LAd and 4.88 mM for LA;2) BCAOH could significantly shorten the survival span, reduce the survival rate and cause neurological deficits,which were associated with high level of lipid hydroperoxide production in cerebral tissues;3) LAd decreased lipid peroxidation and improved the neurological outcome more than LA.It is concluded that LAd offers a better neuroprotection than LA against brain damage caused by cerebral ischemia. | Yihan Wang Chao Zhao Qiujv Wang Huimin Wen Yaru Gu Linlin Fan Chao Ma Maosheng Cheng Yuyang Zhang | 2017 | Asian Journal of Pharmaceutical Sciences2017，12，2： | 1 |
| 7 | Gene cloning and expression of a new acidic family 7 endo-β-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1显示文摘 | Huiying Luo Jun Yang Peilong Yang Jiang Li Huoqing Huang Pengjun Shi Yingguo Bai Yaru Wang Yunliu Fan Bin Yao | 2010 | Applied Microbiology and Biotechnology2010，，4： | 1 |
| 8 | Multiple-model GWAS identifies optimal allelic combinations of quantitative trait loci for malic acid in tomato显示文摘Malic acid(MA)is an important flavor acid in fruits and acts as a mediator in a series of metabolic pathways.It is important to understand the factors affecting MA metabolism for fruit flavor improvement and to understand MA-mediated biological processes.However,themetabolic accumulation of MA is controlled by complex heredity and environmental factors,making it difficult to predict and regulate the metabolism of MA.In this study,we carried out a genome-wide association study(GWAS)on MA using eight milestone models with two-environment repeats.A series of associated SNP variations were identified from the GWAS,and 15 high-confidence annotated geneswere further predicted based on linkage disequilibrium and lead SNPs.The transcriptome data of candidate geneswere explored within different tomato organs as well as various fruit tissues,and suggested specific expression patterns in fruit pericarp.Based on the genetic parameters of population differentiation and SNP distribution,tomato MA content has been more influenced by domestication sweeps and less affected by improvement sweeps in the long-term history of tomato breeding.In addition,genotype×environment interaction might contribute to the difference in domestication phenotypic data under different environments.This study provides new genetic insights into how tomato changed its MA content during breeding and makes available function-based markers for breeding by marker-assisted selection. | Wenxian Gai Fan Yang Liangdan Yuan Saeed ul Haq Yaru Wang Ying Wang Lele Shang Fangman Li Pingfei Ge Haiqiang Dong Jinbao Tao Fei Wang Xingyu Zhang Yuyang Zhang | 2023 | Horticulture Research2023，10，4： | 0 |
| 9 | Dual-engineered cartilage-targeting extracellular vesicles derived from mesenchymal stem cells enhance osteoarthritis treatment via miR-223/NLRP3/pyroptosis axis:Toward a precision therapy显示文摘Osteoarthritis(OA)is the most common disabling joint disease with no effective disease modifying drugs.Extracellular vesicles released by several types of mesenchymal stem cells could promote cartilage repair and ameliorate OA pathology in animal models,representing a novel therapeutic strategy.In this study,we demonstrated that extracellular vesicles derived from human umbilical cord mesenchymal stem cells(hUC-EVs)could maintain chondrocyte homeostasis and alleviate OA,and further revealed a novel molecular mechanism of this therapeutic effect.miR-223,which could directly bind with the 3′UTR of NLRP3 mRNA,was found to be a key miRNA for hUC-EVs to exert beneficial effects on inflammation inhibiting and cartilage protecting.For enhancing the effect on mitigating osteoarthritis,exogenous miR-223 was loaded into hUC-EVs by electroporation,and a collagen II-targeting peptide(WYRGRL)was modified onto the surface of hUC-EVs by genetic engineering to achieve a more targeted and efficient RNA delivery to the cartilage.The dual-engineered EVs showed a maximal effect on inhibiting the NLRP3 inflammasome activation and chondrocyte pyroptosis,and offered excellent results for the treatment of OA.This study provides a novel theoretical basis and a promising therapeutic strategy for the application of engineered extracellular vesicles in OA treatment. | Weixuan Liu Anqi Liu Xujun Li Ziyang Sun Zhenghua Sun Yaru Liu Gang Wang Dan Huang Hao Xiong Shiyang Yu Xintao Zhang Cunyi Fan | 2023 | Bioactive Materials2023，，12： | 0 |
| 10 | Insights into varicella-zoster virus assembly from the B-and C-capsid at near-atomic resolution structures显示文摘Varicella-zoster is a highly communicable virus that can be transmitted through the airborne route.About one quarter of people are infected with this virus.Previous studies have described the structure of A-capsid and a blurred reconstruction of the C-capsid with icosahedral symmetry.In this study,we have determined the more precise detailed structures of the varicella-zoster virus(VZV)B-and C-capsid in icosahedral symmetry using a combination of block-based reconstruction and symmetry relaxation strategies.In addition,we are reporting structural details of the portal vertex reconstructions in five-fold symmetry and portal reconstructions in twelve-fold symmetry.The structures unveil the basis for the high thermal stability of the VZV capsid.The conformational flexibility of structural elements of the capsid plays a role in the assembly of the capsid and drives processes critical for the viral life cycle.The results of the study open up new avenues for the development of drugs against a highly prevalent and contagious pathogen. | Lei Cao Nan Wang Zhe Lv Wenyuan Chen Zhonghao Chen Lifei Song Xueyan Sha Guiqiang Wang Yaling Hu Xiaojun Lian Guoliang Cui Jinyan Fan Yaru Quan Hongrong Liu Hai Hou Xiangxi Wang | 2024 | hLife2024，2，2： | 0 |
| 11 | A bifunctional MXene-modified scaffold for photothermal therapy and maxillofacial tissue regeneration显示文摘Oral squamous cell carcinoma is one of the most common malignant tumours in the oral and maxillofacial regions and is highly malignant and prone to recur despite the development of various effective treatments,including surgery and chemoradiotherapy.Actually,it is difficult to ensure the complete elimination of tumour cells,and maxillofacial bone defects caused by surgery are hard to heal by themselves.In addition,chemoradiotherapy can bring serious side-effects.Therefore,it is imperative to develop a postoperative therapy to kill residual squamous cancer cells and repair bone defects without any side-effects.Here,we prepared a 3D scaffold by a 3D printing technique and freeze-drying method,which contained collagen,silk and hydroxyapatite(CSH)and was functionalized with MXene nanosheets(M-CSH).The considerable photothermal effect with long-term stability can significantly kill squamous CAL-27 cancer cells in vitro and inhibit tumour growth in vivo,increasing the probability of the M-CSH scaffold being applied in the photothermal therapy of oral squamous cell carcinoma.Moreover,the cell proliferation-and osteogenic-related protein expression of mouse embryonic osteogenic precursors(MC3T3-E1)indicated excellent biocompatibility and osteogenic activity of M-CSH scaffolds.The good compression modulus(52.8362.25 kPa)and in vivo bone formation performance made it possible to be used as reconstructive materials for bone defects.This scaffold is likely promising in future tissue engineering,especially for the multifunctional treatment of maxillofacial tumours. | Fengji Li Yanling Yan Yanan Wang Yaru Fan Huiru Zou Han Liu Rui Luo Ruixin Li Hao Liu | 2021 | Regenerative Biomaterials2021，8，6： | 0 |
| 12 | Research on Construction Process of EPS in the Thermal Insulation Building显示文摘 | Xiuli Wu Li zhou Yaru Fan | 2015 | International Journal of Technology Management2015，，6： | 0 |
| 13 | Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli显示文摘 | 张会图 Meng Kun Wang Yaru Luo Huiying Yuan Tiezheng Yang Peilong Bai Yingguo Yao Bin Fan Yunliu | 2007 | High Technology Letters2007，13，1： | 0 |
| 14 | High-performance broadband photodetector based on PtSe_(2)/MoS_(2) heterojunction from visible to near-infrared region显示文摘Broadband photodetectors based on narrow bandgap 2D materials have garnered considerable interest for application in the field of optoelectronic devices.However,their large dark current hinders device performance.In this work,a PtSe_(2)/MoS_(2) heterojunction was fabricated for a broadband photodetector operating within the range of visible to near-infrared.The device exhibited suppressed dark currents with a high rectification ratio of 104.The built-in electric field of the heterojunction promoted carrier separation effectively,and the device achieved excellent photoelectric performance with responsivities of 1.7×10^(3),27.52,and 21 mA/W at 635,785,and 1550 nm wavelengths,respectively.Moreover,the specific detectivities(D^(*))were 2.2×10^(13)Jones(635 nm),3.55×10^(11)Jones(785 nm),and 2.72×10^(8) Jones(1550 nm).The device demonstrated a rise/fall time of 131/241μs under 1550 nm laser illumination.Visible and near-infrared imaging detection was also demonstrated based on the heterojunction device at room temperature.This work sheds light on the remarkable potential of PtSe_(2)/MoS_(2) heterojunctions in the domain of high-performance broadband photodetectors. | Bin WANG Jian YUAN Mengqi CHE Mingxiu LIU Yuting ZOU Junru AN Fan TAN Yaru SHI Nan ZHANG Liujian QI Shaojuan LI | 2024 | Science China(Information Sciences)2024，67，3： | 0 |
| 15 | Domesticated HERV-W env contributes to the activation of the small conductance Ca^(2+)-activated K^(+)type 2 channels via decreased 5-HT4 receptor in recent-onset schizophrenia显示文摘The human endogenous retroviruses type W family envelope(HERV-W env)gene is located on chromosome 7q21-22.Our previous studies show that HERV-W env is elevated in schizophrenia and HERV-W env can increase cal-cium influx.Additionally,the 5-HTergie system and particularly 5-hydroxytryptamine(5-HT)receptors play a prominent role in the pathogenesis and treatment of schizophrenia.5-hydroxytryptamine receptor 4(5-HT4R)agonist can block calcium channels.However,the underlying relationship between HERV-W env and 5-HT4R in the etiology of schizophrenia has not been revealed.Here,we used enzyme-linked immunosorbent assay to detect the concentration of HERV-W env and 5-HT4R in the plasma of patients with schizophrenia and we found that there were decreased levels of 5-HT4R and a negative correlation between 5-HT4R and HERV-W env in schizophrenia.Overexpression of HERV-W env decreased the transcription and protein levels of 5-HT4R but increased small conductance Ca^(2+)-activated K^(+)type 2 channels(SK2)expression levels.Further studies revealed that HERV-w env could interact with 5-HT4R.Additionally,luciferase assay showed that an essential region(-364 to-176 from the transcription start site)in the SK2 promoter was required for HERV-W env-induced SK2 expression.Importantly,5-HT4R participated in the regulation of SK2 expression and promoter activity.Electrophysiological recordings suggested that HERV-Wenv could increase SK2 channel currents and the increase of SK2 currents was inhibited by 5-HT4R.In condusion,HERV-W env could activate SK2 channels via decreased 5-HT4R,which might exhibit a novel mechanism for HERV-Wenv to influence neuronal activity in schizophrenia. | Xiulin Wua Qiujin Yan Lianzhong Liu Xing Xue Wei Yao Xuhang Li Wenshi Li Shuang Ding Yaru Xia Dongyan Zhang Fan Zhu | 2023 | Virologica Sinica2023，38，1： | 0 |
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