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| 1 | The first all-season sample set for mapping global land cover with Landsat-8 data显示文摘We report the world's first all-season training and validation sample sets for global land cover classification with Landsat-8 data.Prior to this,such samples were only available at a single date primarily from the growing season.It is unknown how much limitation such a single-date sample has to mapping global land cover in other seasons of the year.To answer this question,we selected available Landsat-8 images from four seasons and collected training and validation samples from them.We compared the performances of training samples in different seasons using Random Forest algorithm.We found that the use of training samples from any individual season would result in the best overall classification accuracy when validated by samples in the same season.The global overall accuracy from combined best seasonal results was 67.2% when classifying the 11 Level-1 classes in the Finer Resolution Observation and Monitoring of Global Land Cover(FROM-GLC) classification system.The use of training samples from all seasons(named all-season training sample set hereafter) produced an overall accuracy of 67.0%.We also tested classification within 10° latitude 60° longitude zones using all-season training subsample within each zone and obtained an overall accuracy of 70.2%.This indicates that properly grouped subsamples in space can help improve classification accuracies.All the results in this study seem to suggest that it is possible to use an all-season training sample set to reach global optimality with universal applicability in classifying images acquired at any time of a year for global land cover mapping. | Congcong Li Peng Gong Jie Wang Zhiliang Zhu Gregory S. Biging Cui Yuan Tengyun Hu Haiying Zhang Qi Wang Xuecao Li Xiaoxuan Liu Yidi Xu Jing Guo Caixia Liu Kwame O. Hackman Meinan Zhang Yuqi Cheng Le Yu Jun Yang Huabing Huang Nicholas Clinton | 2017 | Science Bulletin2017，62，7： | 21 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | Inlet Recirculation Influence to the Flow Structure of Centrifugal Impeller显示文摘Inlet recirculation is proved as an effective way for centrifugal compressor surge margin extension,and is successively used in some engineering applications.Unfortunately its working mechanism is still not being well understood,which leads to redesigning of inlet recirculation mostly by experience.Also,most study about inlet recirculation is steady to date.It is necessary to study surge margin extension mechanism about inlet recirculation.To expose the mechanism in detail,steady and unsteady numerical simulations were performed on a centrifugal compressor with and without inlet recirculation.The results showed that,with inlet recirculation,the inlet axial velocity is augmented,relative Mach number around blade tip leading edge area is significantly reduced and so is the flow angle.As the flow angle decreased,the incidence angle reduced which greatly improves the flow field inside the impeller.Moreover,inlet recirculation changes the blade loading around blade tip and restrains the flow separation on the blade suction side at the leading edge area.The unsteady results of static pressure around blade surface,entropy at inlet crossflow section and vorticity distributions at near tip span surface indicated that,at near stall condition,strong fluctuation exists in the vicinity of tip area due to the interaction between tip leakage flow and core flow.By inlet recirculation these strong flow fluctuations are eliminated so the flow stability is greatly enhanced.All these improvements mentioned above are the reason for inlet recirculation delays compressor stall.This research reveals the surge margin extension reason of inlet recirculation from an unsteady flow viewpoint and provides important reference for inlet recirculation structure design. | YANG Ce CHEN Shan LI Du YANG Changmao WANG Yidi | 2010 | Chinese Journal of Mechanical Engineering2010，23，5： | 10 |
| 4 | High-energy cathode materials for Li-ion batteries: A review of recent developments显示文摘Lithium ion batteries(LIBs) represent one of the most promising solutions for environmentally friendly transportation such as electric vehicles. The demand for high energy density, low cost and environmentally friendly batteries makes high-capacity cathode materials very attractive for future LIBs. Layered Li NixCoyMnzO2(x+y+z=1), Li-rich oxides and Li-V-O compounds have attracted much attention due to their high capacities in recent years. In this review, we focus on the state-of-the-art research activities related to Li NixCoyMnzO2, Li-rich oxides and Li-V-O compounds, including their structures, reaction mechanisms during cycling, challenges and strategies that have been studied to improve their electrochemical performances. | ZHANG YiDi LI Yi XIA XinHui WANG XiuLi GU ChangDong TU JiangPing | 2015 | Science China(Technological Sciences)2015，58，11： | 7 |
| 5 | Quality control of Semen Ziziphi Spinosae standard decoction based on determination of multi-components using TOF-MS/MS and UPLC-PDA technology显示文摘A sensitive,fast and comprehensive method for the quality assessment of Semen Ziziphi Spinosae(SZS)standard decoction with characterization of its chemical components was developed and validated.UPLC-Q/TOF-MS/MS system was used to identify thirty-six chemical components of SZS standard decoction which included nucleosides,phenolic acids,alkaloids,and flavonoids.Furthermore,a UPLC-PDA method was validated to simultaneously determine adenosine,protocatechuic acid,magnoflorine,catechin,protocatechin,vicenin II,spinosin,kaempferol-3-rutinoside,and 6'''-feruloylspinosin which represent four species of characteristic compounds.The qualitative method had been validated according to Chinese Pharmacopoeia(2015 edition)in terms of lineary,repeatability,recovery and stability for all analytes,with the results showing good precision,accuracy and stability.In conclusion,the method using UPLC combined with MS and PDA provided a novel way for the standardization and identification of SZS standard decoction,and also offered a basis for qualitative analysis and quality assessment of the preparations for SZS standard decoction. | Di Wang Qing Li Ran Liu Huarong Xu Yidi Yin Yifan Wang Huijia Wang Kaishun Bi | 2019 | Journal of Pharmaceutical Analysis2019，9，6： | 6 |
| 6 | Immunotherapy of Epstein-Barr Virus Associated Malignancies Using Mycobacterial HSP70 and LMP2A356-364 Epitope Fusion Protein显示文摘Epstein-Barr virus infection is strongly associated with a number of malignancies.The EBV latent membrane protein 2A has been implicated as one of the most attractive candidates for immunotherapy of related malignancies.In previous studies,the T cell epitopes of LMP2A have been identified systematically.However,the epitope-based vaccine generally meets inefficient immunogenicity when used in vivo directly,which could be overcome by combination with appropriate adjuvants.Heat shock protein is a natural chaperon,which is able to activate the classical major histocompatibility complex class I antigen-processing pathway(cross-presentation).In this study,a minigene encoding LMP2A356-364(FLYALALLL)was genetically fused to the carboxy-terminal of mycobacterial heat shock protein 70.The epitope fusion protein was expressed and purified,and the cross-presentation of LMP2A_(356-364) by monocyte-derived dendritic cells pulsed with the epitope fusion protein was evaluated.Results showed that the epitope fusion protein-pulsed mDCs were much more efficient than the single peptide-pulsed mDCs on CTL activation.Immunization of HLA-A2.1 transgenic mice with MtHsp70-LMP2A_(356-364) generated peptide specific CTL more effectively than a single peptide plus incomplete Freund's adjuvant(IFA).Growth of LMP2A expressing B16 melanoma tumor cells was suppressed in the vaccinated groups.Our results suggested that MtHsp70-LMP2A_(356-364) fusion protein was more effective than the CD8^(+)T cell epitope alone on anti-tumor immunity.As a result,the MtHsp70-LMP2A_(356-364) fusion protein is considered to be a promising candidate vaccine for EBV related malignancies. | Genyan Liu Kun Yao Bing Wang Yun Chen Feng Zhou Yidi Guo Jian Xu Hongzhen Shi | 2009 | Cellular & Molecular Immunology2009，6，6： | 6 |
| 7 | Simultaneously Regulating Uniform Zn^(2+) Flux and Electron Conduction by MOF/rGO Interlayers for High‑Performance Zn Anodes显示文摘Owing to the merits of low cost,high safety and environmental benignity,rechargeable aqueous Zn-based batteries(ZBs)have gained tremendous attention in recent years.Nevertheless,the poor reversibility of Zn anodes that originates from dendrite growth,surface passivation and corrosion,severely hinders the further development of ZBs.To tackle these issues,here we report a Janus separator based on a Zn-ion conductive metal-organic framework(MOF)and reduced graphene oxide(rGO),which is able to regulate uniform Zn2+flux and electron conduction simultaneously during battery operation.Facilitated by the MOF/rGO bifunctional interlayers,the Zn anodes demonstrate stable plating/stripping behavior(over 500 h at 1 mA cm^(−2)),high Coulombic efficiency(99.2%at 2 mA cm^(−2) after 100 cycles)and reduced redox barrier.Moreover,it is also found that the Zn corrosion can be effectively retarded through diminishing the potential discrepancy on Zn surface.Such a separator engineering also saliently promotes the overall performance of Zn|MnO2 full cells,which deliver nearly 100%capacity retention after 2000 cycles at 4 A g^(−1) and high power density over 10 kW kg^(−1).This work provides a feasible route to the high-performance Zn anodes for ZBs. | Ziqi Wang Liubing Dong Weiyuan Huang Hao Jia Qinghe Zhao Yidi Wang Bin Fei Feng Pan | 2021 | Nano-Micro Letters2021，13，5： | 3 |
| 8 | Rabies virus co-localizes with early(Rab5) and late(Rab7) endosomal proteins in neuronal and SH-SY5Y cells显示文摘Rabies virus(RABV) is a highly neurotropic virus that follows clathrin-mediated endocytosis and p H-dependent pathway for trafficking and invasion into endothelial cells. Early(Rab5, EEA1) and late(Rab7, LAMP1) endosomal proteins play critical roles in endosomal sorting, maturity and targeting various molecular cargoes, but their precise functions in the early stage of RABV neuronal infection remain elusive. In this study, the relationship between enigmatic entry of RABV with these endosomal proteins into neuronal and SH-SY5 Y cells was investigated.Immunofluorescence, TCID_(50) titers, electron microscopy and western blotting were carried out to determine the molecular interaction of the nucleoprotein(N) of RABV with early or late endosomal proteins in these cell lines. The expression of N was also determined by down-regulating Rab5 and Rab7 in both cell lines through RNA interference. The results were indicative that N proficiently colocalized with Rab5/EEA1 and Rab7/LAMP1 in both cell lines at 24 and 48 h post-infection, while N titers significantly decreased in early infection of RABV. Down-regulation of Rab5 and Rab7 did not inhibit N expression, but it prevented productive infection via blocking the normal trafficking of RABV in a low pH environment. Ultrathin sections of cells studied by electron microscope also verified the close association of RABV with Rab5 and Rab7 in neurons. From the data it was concluded that primary entry of RABV strongly correlates with the kinetics of Rab-proteins present on early and late vesicles, which provides helpful clues to explain the early events of RABV in nerve cells. | Waqas Ahmad Yingying Li Yidi Guo Xinyu Wang Ming Duan Zhenhong Guan Zengshan Liu Maolin Zhang | 2017 | Virologica Sinica2017，32，3： | 3 |
| 9 | Research on High Temperature and High Density White Oil Based Drilling Fluid and Its Application in Well 201H7-6显示文摘As the depth and horizontal length of shale gas development wells increase,the requirement for the temperature resistance and the ability of the drilling fluids to stabilize the shale formation becomes higher.A new type of high temperature and high density white oil based drilling fluid system has been developed in laboratory.Research shows that the drilling fluid system has good rheological property,low filtration loss,strong anti-debris pollution capability and good plugging performance at high temperature and high density.The system has been successfully applied in the 201H7-6 well.Application results show that the drilling fluid rheology,high temperature and high pressure fluid loss and demulsification voltage meet the field requirements.The drilling fluid performance is stable in drilling an 1800 m horizontal section,and no stuck or shale swelling and wellbore collapsing are induced.It is the first well drilled successfully with domestic white oil-based drilling fluid in Zigong Region.It also sets several new records including the deepest well,the shortest drilling cycle,and the fastest drilling speed in that region,which provides valuable experience for the future drilling activities. | Bobo Qin Yidi Wang Chunzhi Luo Gang Liu | 2019 | Open Journal of Yangtze Oil and Gas2019，4，3： | 2 |
| 10 | Effects of shape and particle size on the photocatalytic kinetics and mechanism of nano-CeO_(2)显示文摘Nanomaterials have been widely applied to many fields because of their excellent photocatalytic performance.The performance is closely related to the catalytic kinetics,but it is not completely clear about the influencing regularities of shape and particle size on the photocatalytic kinetics of nanomaterials and the photocatalytic kinetic mechanism.In this paper,nano-CeO_(2)with different shapes and particle sizes were prepared,the kinetic parameters of adsorption and photocatalytic degradation were determined,and the effects of shape and particle size on the kinetics of adsorption and photocatalysis and photocatalytic mechanism were discussed.The results show that the shape and particle size have significant influences.With the decreases of diameter,the performances of adsorption and photocatalysis of nano-CeO_(2)are improved;and these performances of spherical nano-CeO_(2)are greater than those of linear nano-CeO_(2).The shape and particle size have no effects on the kinetic order and mechanism of the whole photocatalytic process.Then a generalized mechanism of photocatalytic kinetics of nanomaterials was proposed and the mechanism rate equation was derived.Finally,the conclusion can be drawn:the desorption of photodegradation products is the control step of photocatalytic kinetics,and the kinetic order of photocatalytic degradation reaction is 1.The mechanism is universal and all nanomaterials have the same photocatalytic kinetic mechanism and order. | Zixiang Cui Lu Zhang Yongqiang Xue Ya’nan Feng Mengying Wang Jiaojiao Chen Boteng Ji Chenyu Wang Yidi Xue | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，12： | 1 |
| 11 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 12 | A Prototype for Diagnosis of Psoriasis in Traditional Chinese Medicine显示文摘Psoriasis is a chronic,non-communicable,painful,disfiguring and disabling disease for which there is no cure,with great negative impact on patients’quality of life(QoL).Diagnosis and treatment with traditional Chinese medical technique based on syndrome differentiation has been used in practice for a long time and proven effective,though,up to now,there are only a few available studies about the use of semantic technologies and the knowledge systems that use Traditional Chinese Medicine(TCM)-syndrome differentiation for information retrieval and automated reasoning.In this paper we use semantic techniques based on ontologies to develop a prototypical system for the diagnosis of Psoriasis.For this purpose,a domain ontology is developed for syndrome differentiation of psoriasis vulgaris(PV).This ontology is founded on an adapted version of the general formal ontology(GFO),with the evidence-based clinical practice guideline of TCM for psoriasis vulgaris(Guideline 2013)as the primary data sources.The implemented prototype,called ONTOPV,contains this domain ontology and is aimed at a decision support system for diagnosis and treatment of PV.This system uses a case-database for Case Based Reasoning(CBR),combined with fuzzy pattern recognition.Experimental results show that the ONTOPV realizes the basic functionalities of data collection,querying,browsing and navigation,and supports rule-based knowledge reasoning,and integrates fuzzy pattern recognition.It can provide users with clinical decision support for TCM syndrome differentiation in diagnosis of psoriasis. | Hai Long Zhe Wang Yidi Cui Junhui Wang Bo Gao Chao Chen Yan Zhu Heinrich Herre | 2022 | Computers, Materials & Continua2022，，12： | 1 |
| 13 | In situ formation of lithiophilic Li_(22)Sn_(5) alloy and high Li-ion conductive Li_(2)S/Li_(2)Se via metal chalcogenide SnSSe for dendrite-free Li metal anodes显示文摘Lithium metal has gained extensive attention as the most ideal candidate for next-generation battery anode owing to the ultrahigh specific capacity and the lowest electrochemical potential.However,uncontrollable dendrite growth and huge volume variation extremely restrict the future deployment of lithium metal batteries.Herein,we report metal chalcogenide SnSSe with unique nanoplate stacking structure as a robust substrate for stable Li metal anode.During the initial Li plating process,lithiophilic Li_(22)Sn_(5) alloy and Li_(2)S/Li_(2)Se sites are obtained via in-situ electrochemical reaction of Li metal and SnSSe.Density functional theory(DFT)calculation demonstrates that the formed Li_(2)S/Li_(2)Se achieves low Li diffusion energy barrier,ensuring rapid Li~+migration.Li_(22)Sn_(5) alloy provides strong nucleation sites,promoting uniform Li nucleation.Furthermore,in-situ optical microscopy analysis suggests that the synthesized effect fundamentally inhibits lithium dendrite growth.Consequently,SnSSe modified Cu foil delivered an ultralow nucleation overpotential,superior cycling stability with 450 cycles(Coulombic efficiency,>98%),and excellent plating/stripping behavior over 2200 h at 0.5 mA cm^(-2).Moreover,the brilliant reversible cycles and rate capability were also realized in Li@SnSSe//LiFePO_(4)(LFP)full cell,shedding light on the feasibility of SnSSe for stable and dendrite-free lithium metal anode. | Yaya Wang Yang Guo Jiang Zhong Meng Wang Lei Wang Shengyang Li Song Chen Hongli Deng Yong Liu Yidi Wu Jian Zhu Bingan Lu | 2022 | Journal of Energy Chemistry2022，31，10： | 0 |
| 14 | Application of nitrogen loaded biochar in purifying agricultural wastewater and as a nitrogen releaser for rice production显示文摘Herein a new approach to the application of agricultural eutrophic wastewater for rice plant cultivation is described.Biochar was used as a medium for the sorption of ammonia from simulated wastewater and subsequently as a nitrogen(N)releaser in the cultivation of rice plants.The main goals of this approach were to isolate ammonia from simulated wastewater and transfer it into rice cultivation,and or explore how exogenous N promoted the growth of rice.The results demonstrate that according to X-ray diffraction phase analysis,most of the properties of biochar were retained before and after loading NH_(4)^(+)-N.Compared with biochar,the crystal peak of AlOOH in N-loaded biochar(NLB)disappeared and the intensity of the crystal peak of CuCaSe_(2)decreased,which was the important mechanism allowing it to adsorb 30.8%of the N present in simulated low N-concentration agricultural wastewater.The soil N content in NLB treatments was higher than in Non-NLB treatments during the critical tillering and reproductive growth stages.Moreover,the N adsorption-desorption process of NLB matched the N requirements of the rice plant,and thus greatly increased the tiller number by 11.9%and rice yield by 7.5%.These results indicated that the indirect use of ammonia derived from wastewater using biochar as a sorption and releasing medium for rice plant cultivation was promising.This is the first time that biochar was used for possibly indirect reuse of agricultural eutrophic wastewater and enhancement of rice plant growth. | Hongyang Chen Yidi Sun Yang Sun Yanzhi Wang Yanqi Li Qi Wu Daocai Chi | 2023 | International Journal of Agricultural and Biological Engineering2023，16，4： | 0 |
| 15 | Audio-visual keyword transformer for unconstrained sentence-level keyword spotting显示文摘As one of the most effective methods to improve the accuracy and robustness of speech tasks,the audio-visual fusion approach has recently been introduced into the field of Keyword Spotting(KWS).However,existing audio-visual keyword spotting models are limited to detecting isolated words,while keyword spotting for unconstrained speech is still a challenging problem.To this end,an Audio-Visual Keyword Transformer(AVKT)network is proposed to spot keywords in unconstrained video clips.The authors present a transformer classifier with learnable CLS tokens to extract distinctive keyword features from the variable-length audio and visual inputs.The outputs of audio and visual branches are combined in a decision fusion module.As humans can easily notice whether a keyword appears in a sentence or not,our AVKT network can detect whether a video clip with a spoken sentence contains a pre-specified keyword.Moreover,the position of the keyword is localised in the attention map without additional position labels.Exper-imental results on the LRS2-KWS dataset and our newly collected PKU-KWS dataset show that the accuracy of AVKT exceeded 99%in clean scenes and 85%in extremely noisy conditions.The code is available at http://gffzz188fe103f8f1460as0puoubfupuwu69kn.ffgz.tsg.suse.edu.cn/jialeren/AVKT. | Yidi Li Jiale Ren Yawei Wang Guoquan Wang Xia Li Hong Liu | 2024 | CAAI Transactions on Intelligence Technology2024，9，1： | 0 |
| 16 | Unraveling the skatole biodegradation process in an enrichment consortium using integrated omics and culture-dependent strategies显示文摘3-Methylindole(skatole)is regarded as one of the most offensive compounds in odor emission.Biodegradation is feasible for skatole removal but the functional species and genes responsible for skatole degradation remain enigmatic.In this study,an efficient aerobic skatole-degrading consortium was obtained.Rhodococcus and Pseudomonas were identified as the two major and active populations by integrated metagenomic and metatranscriptomic analyses.Bioinformatic analyses indicated that the skatole downstream degradation wasmainly via the catechol pathway,and upstream degradation was likely catalyzed by the aromatic ring-hydroxylating oxygenase and flavin monooxygenase.Genome binning and gene analyses indicated that Pseudomonas,Pseudoclavibacter,and Raineyella should cooperate with Rhodococcus for the skatole degradation process.Moreover,a pure strain Rhodococcus sp.DMU1 was successfully obtained which could utilize skatole as the sole carbon source.Complete genome sequencing showed that strain DMU1 was the predominant population in the consortium.Further crude enzyme and RT-qPCR assays indicated that strain DMU1 degraded skatole through the catechol ortho-cleavage pathway.Collectively,our results suggested that synergistic degradation of skatole in the consortium should be performed by diverse bacteria with Rhodococcus as the primary degrader,and the degradation mainly proceeded via the catechol pathway. | Qiao Ma Nan Meng Jiancheng Su Yujie Li Jiazheng Gu Yidi Wang Jingwei Wang Yuanyuan Qu Zelong Zhao Yeqing Sun | 2023 | Journal of Environmental Sciences2023，，5： | 0 |
| 17 | Recent progress in co-detection of single-cell transcripts and proteins显示文摘Cellular heterogeneity is a universal property of living systems,and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological processes.Co-analysis of transcripts and proteins from the same single cells opens the way to decipher complex RNA regulatory frameworks and phenotypes,facilitating the understanding of cellular fate and function regulations,discovery of novel cell types,and construction of a high-resolution cell atlas.Herein,we review the state-of-art advances in the development of methodologies for co-analysis of single-cell transcripts and proteins.First,imaging-based methods are summarized with particular emphasis on optical and mass spectrometry imaging.Next,sequencing-based approaches for high-throughput and sensitive co-analysis of single-cell transcripts and proteins are described,including droplet-,microwell-,and split-pool-based platforms.Subsequently,combined methods with more flexibility and universality are discussed.These methods commonly employ different strategies or reactions to convert transcripts and proteins of single cells into distinct signals simultaneously,which can be detected by different instruments or platforms.Lastly,some perspectives on the future challenges and development trends in this field are presented. | Shanqing Huang Qian Fan Yidi Wang Zhi Huang Weixiong Shi Yanli Gong Ting Yang Jie Wang Lingling Wu Chaoyong Yang | 2024 | Nano Research2024，17，1： | 0 |
| 18 | Comparative study on Photobiomodulation between 630 nm and 810 nm LED in diabetic wound healing both in vitro and in vivo显示文摘Photobiomodulation(PBM)promoting wound healing has been demonstrated by many studies.Currently,630 nm and 810 nm light-emitting diodes(LEDs),as light sources,are frequently used in the treatment of diabetic foot ulcers(DFUs)in clinics.However,the dose-effect relationship of LED-mediated PBM is not fully understood.Furthermore,among the 630 nm and 810 nm LEDs,which one gets a better effect on accelerating the wound healing of diabetic ulcers is not clear.The aim of this study is to evaluate and compare the effects of 630 nm and 810 nm LED-mediated PBM in wound healing both in vitro and in vivo.Our results showed that both 630 nm and 810 nm LED irradiation significantly promoted the proliferation of mouse fibroblast cells(L929)at different light irradiances(1,5,and 10 mW/cm^(2)).The cell proliferation rate increased with the extension of irradiation time(100,200,and 500 s),but it decreased when the irradiation time was over 500 s.Both 630 nm and 810nm LED irradiation(5 mW/cm^(2))significantly improved the migration capability of L929 cells.No difference between 630 nm and 810 nm LED-mediated PBM in promoting cell proliferation and migration was detected.In vivo results presented that both 630 nm and 810 nm LED irradiation promoted the wound healing and the expression of the vascular endothelial growth factor(VEGF)and transforming growth factor(TGF)in the wounded skin of type 2 diabetic mice.Overall,these results suggested that LED-mediated PBM promotes wound healing of diabetic mice through promoting fibroblast cell proliferation,migration,and the expression of growth factors in the wounded skin.LEDs(630 nm and 810 nm)have a similar outcome in promoting wound healing of type 2 diabetic mice. | Hongyou Zhao Tengda Ji Tianzhen Sun Haolin Liu Yidi Liu Defu Chen Ying Wang Yizhou Tan Jing Zeng Haixia Qiu Ying Gu | 2022 | Journal of Innovative Optical Health Sciences2022，15，2： | 0 |
| 19 | Long-term follow-up of volumetric modulated arc therapy in definitive radiotherapy for cervical cancer: A single-center retrospective experience显示文摘Objective:To evaluate the treatment outcomes and toxicity of volumetric modulated arc therapy(VMAT)in definitive radiotherapy for patients with cervical cancer.Methods:Medical records of 440 patients with cervical cancer treated with VMAT in definitive radiotherapy with or without concurrent chemotherapy between January 2010 and June 2015 in our institution were retrospectively reviewed.A dose of 45 Gy was prescribed to the planning target volume with VMAT in 25 fractions,followed by high-dose rate intracavitary brachytherapy with 28–36 Gy in 4–6 fractions.Survival time was estimated using Kaplan-Meier method.Risk factors for treatment failure were identified using Cox proportional hazards regression model.Results:The median follow-up periods were 58.3 months(range:2.7–112.9 months).The 3-and 5-year survival rates were overall survival(OS)79.4%and 74.4%,cancer-specific survival(CSS)81.4%and 77.1%,progressionfree survival(PFS)73.3%and 71.1%,locoregional control(LC)83.3%and 82%and distant metastasis-free survival(DMFS)80.8%and 77.7%,respectively.The 5-year OS for patients with different stages was IB191.6%,IB2100%,IIA 82.5%,IIB 74.4%,IIIA 57.1%,IIIB 61.7%and IVB 17.6%,respectively.Of the 124 patients with recurrence,93 patients(75.0%)experienced distant failure.Multivariate analysis showed that non-squamous cell carcinoma of the cervix(P<0.001),more advanced stage(P=0.004),pelvic lymph node metastasis(P<0.001)and para-aortic lymph node metastasis(P=0.019)were all independent risk factors of PFS.The incidence of grades 3–4 late bowel toxicities was 2.7%.No grades 3–4 bladder toxicity was observed.Conclusion:This study demonstrated that the use of VMAT in definitive radiotherapy resulted in satisfactory outcomes with acceptable toxicities for patients with cervical cancer.Distant metastasis predominated as the pattern of disease relapse.Further studies are needed to investigate the intensified systemic therapies. | Yidi Yuan Jing You Weihu Wang Xiaofan Li | 2020 | Radiation Medicine and Protection2020，1，2： | 0 |
| 20 | Physical dosimetric reconstruction of a case of large area back skin injury due to overexposure in an interventional procedure显示文摘To estimate the physical dose of skin and key organs in a case of overexposure during a cardiac interventional procedure.Methods The female patient aged 50 suffered from owerexposure during ardiac interventional therapy in a hospital,Xinxiang city,Henan province,China in January 2020.The mesh-type phantom for the patient was constructed based on the adult mesh-type reference computational phantoms(MRCPs)released by the International Comission on Radiological Protection Publication 145 (ICRP145)and phantom deformation technology.Models of exposure scenario were constructed and simulated with particle and heavy ion transport code system(PHTTS)according to exposure conditions.Resuts:The maximum absorbed dose of key organs/tissues under iradiation in posteroanterior(PA)and 30°left anterior oblique directions(LOA)was 632.4 and 305.6 mGy,respectively.The let lung,heart,and left mammary gland received a larger dose under both iradiation conditions.The ratio of the absorbed dose with and without shielding was a lculated,and the relative difference in most organs was<1%between two directions.The iso-dose curve of the back skin revealed the ditribution of the absorbed dose(0.1-5.2 Gy).The dose estimate of key tssues/organs was higher than the conventional level,especially the local skin,up to 5.2 Gy.Concusions The interventional procedure in this ase resulted in a higher dose.Monte Carlo codes combined with the MRCPs can be employed to estimate physical dose to individuals in concrete irradia tion scenarios. | Yuchen Yin Xuan Wang Xianghui Kong Wenyue Zhang Yidi Wang Yuxuan Mao Jianwei Wang Tianhe Jia Yu Tu Bingjie Zhang Liang Sun | 2022 | Radiation Medicine and Protection2022，3，1： | 0 |
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