| 1 | Identifi cation of targets and mechanisms for Eleutheroside E in the treatment of cancer显示文摘Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been reported.In this study,we used bioinformatics to determine the role and mechanisms of EE in inhibiting breast cancer(BRCA),prostate cancer(PRAD),lung cancer(LUAD),and bladder cancer(BLCA).Finally,the MTT method was used to evaluate the inhibitory effect of EE on breast cancer MDA-MB-231 cells,and the target genes were determined by qRT-PCR.The results showed that the survival rate of MDA-MB-231 cells was reduced by 27.33%after 400μg/mL EE treatment.The results of qRT-PCR showed that EE down-regulated target genes in breast cancer cells,which was consistent with our predicted results.Moreover,our results indicated that EE may have impact on the transcription and translation of BLCA cells by targeting ADCY2,ADCY5 and JUN.In BRCA,we identified three targets for EE.In addition,EE affected DNA repair and the development of BRCA by affecting the expression of RAD51.In LUAD,we identified 9 targets for EE,8 of which were related to DNA repair.EE inhibited PRAD by targeting ADCY8.Our research was the first report on the targets and mechanisms of EE for suppressing cancer combined with clinical data.Our research also provides theoretical support for using EE to suppress cancer. | Deyong Zeng Yi Xiong Yishu Yin Shan Shan Fangyuan Duan Xin Gao Chen Song Mengyao Liu Yingchun Zhang Weihong Lu | 2022 | Journal of Future Foods2022，2，1： | 0 |
| 2 | Effects of Space Flight on Expression of Key Proteins in Rice Leaves显示文摘As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth-and development-related physiological processes and metabolic pathways of rice different stages, the seeds of rice variety DN423 were sent into orbit for 12.5 d aboard the SJ-10 Returning Satellite, and then the seedlings of both treated and control rice were compared at the three-leaf stage(TLS) and tillering stage(TS). In addition to comparing plant growth and reactive oxygen species(ROS) levels, seedling proteomes were also compared using isobaric tags for relative and absolute quantitation(i TRAQ). Space flight increased TLS plant height by 20%, reduced and increased ROS levels of the TLS and TS seedlings, respectively, and affected the expression of 36 and 323 proteins in TLS and TS leaves, respectively. Furthermore, the functions of the differentially abundant proteins were mainly associated with metabolism, energy, and protein synthesis and degradation. These results suggested that the exposure of seeds to outer space conditions affects the subsequent abundance of key signaling proteins, gene expression, and the processes of protein synthesis and degradation, thereby affecting metabolic processes and promoting adaptation to the abiotic stress of outer space. As such, the present study sheds light on the effects of space flight on plants and contributes to a more comprehensive understanding of extraterrestrial biology. | Zeng Deyong Cui Jie Yin Yishu Zhang Meng Shan Shan Gao Xin Zhang Yingchun Sun Yeqing Lu Weihong | 2020 | Rice science2020，27，5： | 0 |
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