|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Ripple-associated high-firing interneurons in the hippocampal CA1 region显示文摘By simultaneously recording the activity of individual neurons and field potentials in freely behaving mice, we found two types of interneurons firing at high frequency in the hippocampal CA1 region, which had high correlations with characteristic sharp wave-associated ripple oscillations (100―250 Hz) during slow-wave sleep. The firing of these two types of interneurons highly synchronized with ripple oscillations during slow-wave sleep, with strongly increased firing rates corresponding to individual ripple episodes. Interneuron type I had at most one spike in each sub-ripple cycle of ripple episodes and the peak firing rate was 310±33.17 Hz. Interneuron type II had one or two spikes in each sub-ripple cycle and the peak firing rate was 410±47.61 Hz. During active exploration, their firing was phase locked to theta oscillations with the highest probability at the trough of theta wave. Both two types of interneurons increased transiently their firing rates responding to the startling shake stimuli. The results showed that these two types of high-frequency interneurons in the hippocampal CA1 region were involved in the modulation of the hippocampal neural network during different states. | WANG Ying, ZHANG Lu, PAN JingWei, XIE Kun, LI ShiQi, WANG ZhiRu & LIN LongNian Key Laboratory of Brain Functional Genomics of Ministry of Education, East China Normal University, Shanghai 200062, China | 2008 | Science China(Life Sciences)2008，51，2： | 3 |
| 2 | Mechanical Properties of Sea Water Sea Sand Coral Concrete Modified with Different Cement and Fiber Types显示文摘The mechanical properties of modified sea water sea sand coral concrete(SWSSCC)under axial compression were experimentally studied.Two different parameters were considered in this test:types of cement and fiber.An experimental campaign was developed involving uniaxial compression tests and the use of digital image correlation(DIC)method to analyze the strain distribution and crack propagation of specimen.Test results indicated that the compressive strength and elastic modulus of SWSSCC were improved by adding stainless steel fibers(SSF),while polypropylene fibers(PF)enhanced the SWSSCC peak deformation.It was found that the elastic modulus and strength of SWSSCC using ordinary Portland cement(OPC)were higher compared to specimen with low alkalinity sulphoaluminate cement(LAS).Typical strain distribution changed with the variation of fiber types.The propagation and characteristics of cracks in SWSSCC containing PF were similar to those of cracks in SWSSCC.However,the propagation of cracks and the development of plastic deformation in SWSSCC were effectively hindered by adopting SSF.Finally,an analytical stress-strain expression of specimen considering the influences of fibers was established.The obtained results would provide a basis for the application of SWSSCC. | Xibo Qi Yijie Huang Xiaowei Li Zhenhua Hu Jingwei Ying Dayong Li | 2020 | Journal of Renewable Materials2020，8，8： | 2 |
| 3 | Modified DIX model for ion-exchange equilibrium of L-phenylalanine on a strong cation-exchange resin显示文摘L-phenylalanine,one of the nine essential amino acids for the human body,is extensively used as an ingredient in food,pharmaceutical and nutrition industries.A suitable equilibrium model is required for purification of L-phenylalanine based on ion-exchange chromatography.In this work,the equilibrium uptake of L-phenylalanine on a strong acid-cation exchanger SH11 was investigated experimentally and theoretically.A modified Donnan ion-exchange(DIX) model,which takes the activity into account,was established to predict the uptake of L-phenylalanine at various solution pH values.The model parameters including selectivity and mean activity coefficient in the resin phase are presented.The modified DIX model is in good agreement with the experimental data.The optimum operating pH value of 2.0,with the highest L-phenylalanine uptake on the resin,is predicted by the model.This basic information combined with the general mass transfer model will lay the foundation for the prediction of dynamic behavior of fixed bed separation process. | Jinglan wu Pengfei Jiao Wei Zhuang Jingwei Zhou Hanjie Ying | 2016 | Chinese Journal of Chemical Engineering2016，24，10： | 2 |
| 4 | Five- phase composite sphere model for chloride diffusivity predic- tion of recycled aggregate concrete显示文摘 | Ying Jingwei Xiao Jianzhuang Shen Luming | 2013 | Magazine of Concrete Research2013，65，9： | 1 |
| 5 | Transformation of microstructure and phase of disodium guanosine 5′-monophosphate: Thermodynamic perspectives显示文摘Microstructure and phase transformation of disodium guanosine 5′-monophosphate(5′-GMPNa_2) are extremely important for controlling the process and understanding the mechanism of crystallization. In this work, the thermodynamic properties of polymorphous 5′-GMPNa_2 especially the solubility were studied, the solubility results show that 5′-GMPNa_2 is more soluble in ethanol–water(E–W) than in isopropanol–water(I–W). The amorphous form of 5′-GMPNa_2 is more soluble than the crystalline form at the same mole fraction and temperature. Meanwhile, the crystalline forms and morphologies of the residual solids were characterized by PXRD and SEM. The results indicate that solid forms of 5′-GMPNa_2 transformed spontaneously from amorphous to crystalline when the ethanol proportion is ≥20%. In addition, increasing the pH facilitates the dissolution of 5′-GMPNa_2 and helps to maintain the crystalline form. The associated Gibbs free energy values were calculated to verify the trend of transformation from amorphous to crystalline 5′-GMPNa_2. These results should help to guide the industrial crystallization process and to obtain the crystalline form of 5′-GMPNa_2. | Qiao Chen Fengxia Zou Pengpeng Yang Jingwei Zhou Jinglan Wu Wei Zhuang Hanjie Ying | 2018 | Chinese Journal of Chemical Engineering2018，26，10： | 1 |
| 6 | The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing显示文摘A thorough interrogation of the immune landscape is crucial for immunotherapy strategy selection and prediction of clinical responses in non-small-cell lung cancer(NSCLC)patients.Single-cell RNA sequencing(scRNA-seq)techniques have prompted the opportunity to dissect the distinct immune signatures between lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC),the two major subtypes of NSCLC.Here,we performed scRNA-seq on 72,475 immune cells from 40 samples of tumor and matched adjacent normal tissues spanning 19 NSCLC patients,and drew a systematic immune cell transcriptome atlas.Joint analyses of the distinct cellular compositions,differentilly expressed genes(DEGs),cell-cell interactions,pseudotime trajectory,transcriptomic factors and prognostic factors based on The Cancer Genome Atlas(TCGA),revealed the central roles of cytotoxic and effector T and NK cells and the distinct functional macrophages(Mo)subtypes in the immune microenvironment heterogeneity between LUAD and LUSC.The dominant subtype of Mo was FABP4-Mp in LUAD and SPP1-Mo in LUSC.Importantly,we identifieda novel lymphocyte-related Mo cluster,which we named SELENOP-Mo,and further established its antitumor role in both types,especially in LUAD.Our comprehensive depiction of the immune heterogeneity and definition of Mp clusters could help design personalized treatment for lung cancer patients in clinical practice. | Chengdi Wang Qiuxiao Yu Tingting Song Zhoufeng Wang Lujia Song Ying Yang Jun Shao Jingwei Li Yinyun Ni Ningning Chao Li Zhang Weimin Li | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 1 |
| 7 | Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neurons显示文摘The expression of linear DNA sequence is precisely regulated by the three-dimensional(3D)architecture of chromatin.Morphine-induced aberrant gene networks of neurons have been extensively investigated;however,how morphine impacts the 3D genomic architecture of neurons is still unknown.Here,we applied digestion-ligation-only high-throughput chromosome conformation capture(DLO Hi-C)technology to investigate the effects of morphine on the 3D chromatin architecture of primate cortical neurons.After receiving continuous morphine administration for 90 days on rhesus monkeys,we discovered that morphine re-arranged chromosome territories,with a total of 391 segmented compartments being switched.Morphine altered over half of the detected topologically associated domains(TADs),most of which exhibited a variety of shifts,followed by separating and fusing types.Analysis of the looping events at kilobase-scale resolution revealed that morphine increased not only the number but also the length of differential loops.Moreover,all identified differentially expressed genes from the RNA sequencing data were mapped to the specific TAD boundaries or differential loops,and were further validated for changed expression.Collectively,an altered 3D genomic architecture of cortical neurons may regulate the gene networks associated with morphine effects.Our finding provides critical hubs connecting chromosome spatial organization and gene networks associated with the morphine effects in humans. | Liang Wang Xiaojie Wang Chunqi Liu Wei Xu Weihong Kuang Qian Bu Hongchun Li Ying Zhao Linhong Jiang Yaxing Chen Feng Qin Shu Li Qinfan Wei Xiaocong Liu Bin Liu Yuanyuan Chen Yanping Dai Hongbo Wang Jingwei Tian Gang Cao Yinglan Zhao Xiaobo Cen | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 8 | Evolving pattern and improvement path of China’s solid waste management policies显示文摘Systematically sorting out the evolving pattern of solid waste management policies and studying the key points and development trends of solid waste management policies are conducive to the clarification of improvement path of solid waste management policies.Based on the promulgated solid waste management policies,this study applies statistical methods to the analysis of the text structure and contents of the solid waste management policies,attempting to find out the internal evolving pattern of the solid waste management policies in China.Closely integrating the current policy requirements for the construction of‘waste-free city’,this study puts forward a policy optimization and improvement path.The findings are briefly listed blow:①The solid waste management policies have demonstrated multiple text types and various levels of effectiveness,and the policy framework system is gradually optimized.②In each edition of solid waste management policy,the hotspot issues are highlighted with distinctive characteristics of the times and dynamic evolution.The policies are covering more fields,the contents are more targeted and comprehensive,and the implementation is more specific and practical.③The policies mainly focus on harmlessness,reduction,reclamation,pollution prevention,supervision and management,secondary pollution,recycling,circular economy,etc.④The government strengthens the top-level design from the macro level,focusing on policy guidance and standardization,policy coordination,technical support,and governance improvement to promote the realization of the goals of‘harmlessness,reduction,reclamation’.⑤The diversification of tools,approaches and factors supporting solid waste management,and the growing awareness of the technical support of solid waste management,and the management mode and governance path have entered the phase of adaptive transformation.Based on practical needs,the market oriented policies and incentive mechanisms for solid waste management are inadequate,applicable technical attention is not highly paid,and the comprehensive management level and governance capabilities are in urgent need of improvement.Combined with the theoretical research,practical actions and policy initiatives of the construction of‘waste-free city’in other countries,from the perspectives of solid waste full product lifecycle management,technical support paradigm policy,main body coordination policy,guidance incentive policy,laws and regulations and other aspects,the policy improvement path and direction of China’s‘waste-free city’are thoroughly discussed in this study. | Zhixiong Tan Ying Ren Jingwei Han Siying Chen | 2021 | Chinese Journal of Population,Resources and Environment2021，19，4： | 0 |
| 9 | High-Throughput Drug Screening on Borrelia garinii and Borrelia afzelii Identified Hypocrellin A as an Active Drug Candidate Against Borrelia Species显示文摘Lyme disease(LD)is a tick-transmitted infection caused by Borrelia burgdorferi sensu lato species,which include B.burgdorferi,Borrelia afzelii and Borrelia garinii.The majority of patients with early LD can be cured by the standard treatment,yet some still suffer from posttreatment LD syndrome.The presence of Borrelia persisters has been proposed as a contributing factor,because they cannot be completely eradicated by the currently used antibiotics for LD.Finding new pharmaceuticals targeting Borrelia persisters is crucial for developing more effective treatments.Here,we first confirmed the existence of persisters in B.garinii and B.afzelii cultures and then conducted a high-throughput screening of a customdrug library against persister-rich stationary-phase B.garinii and B.afzelii cultures.Among 2427 compounds screened,hypocrellin A(HA),anthracycline class of drugs and topical antibiotics along with some other natural compounds were identified to have strong potential for killing persisters of B.garinii and B.afzelii.HA was the most active anti-Borrelia compound,capable of eradicating stationary-phase Borrelia persisters,in particular when combined with doxycycline and/or ceftriaxone.Liposoluble antioxidant vitamin E was found to antagonize the activity of HA,indicating HA’s target is the cell membrane where HA triggers the generation of reactive oxygen species in the presence of light.HA was found to have distinct bactericidal activity against Borrelia species but had poor or no activity against gram-positive and gram-negative bacteria.Identification of the abovementioned drug candidates may help develop more effective therapies for LD. | Tingting Li Yuxian Xin Dongxia Liu Jingrong Sun Jingwei Li Ying Zhang Jie Feng | 2023 | Infectious Microbes & Diseases2023，5，2： | 0 |
| 10 | Highly expressed SERCA2 triggers tumor cell autophagy and is a druggable vulnerability in triple-negative breast cancer显示文摘Chemoresistance remains a major obstacle to successful treatment of triple negative breast cancer(TNBC).Identification of druggable vulnerabilities is an important aim for TNBC therapy.Here,we report that SERCA2 expression correlates with TNBC progression in human patients,which promotes TNBC cell proliferation,migration and chemoresistance.Mechanistically,SERCA2 interacts with LC3B via LIR motif,facilitating WIPI2-independent autophagosome formation to induce autophagy.Autophagy-mediated SERCA2 degradation induces SERCA2 transactivation through a Ca^(2+)/CaMKK/CREB-1 feedback.Moreover,we found that SERCA2-targeting small molecule RL71 enhances SERCA2-LC3B interaction and induces excessive autophagic cell death.The increase in SERCA2 expression predisposes TNBC cells to RL71-induced autophagic cell death in vitro and in vivo.This study elucidates a mechanism by which TNBC cells maintain their high autophagy activity to induce chemoresistance,and suggests increased SERCA2 expression as a druggable vulnerability for TNBC. | Minmin Fan Jian Gao Lin Zhou Wenwen Xue Yixuan Wang Jingwei Chen Wuhao Li Ying Yu Bo Liu Yan Shen Qiang Xu | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 0 |
| 11 | Long term performance of recycled concrete beams with different water-cement ratio and recycled aggregate replacement rate显示文摘The purpose of this study is to reveal the service performance of recycled aggregate concrete(RAC)components for different values of water-cement ratio and replacement rate of recycled coarse aggregate(RCA).Generally,the concrete strength decreases with the increase of the replacement rate of RCA,in order to meet the strength requirements when changing the replacement rate of RCA,it is necessary to change the water-cement ratio at the same time.Therefore,the axial compressive strengths of prism with 25 mix proportions,the short-term mechanical properties and long-term deformation properties of reinforced concrete beams were tested respectively by changing water-cement ratio and RCA replacement rate.The bearing capacity and the strain nephogram of samples under different loads were obtained using the Digital Image Correlation(DIC)method,and a self-made gravity loading experimental device was used for long-term deformation investigation.Results showed that the damage pattern of RAC was the same as that of natural aggregate concrete(NAC),but the brittleness was more pronounced.The brittleness of concrete before failure can be reduced more effectively by adjusting the replacement rate of RCA than by adjusting the water-cement ratio.The water-cement ratio has an evident influence on the axial compressive strength and early creep of concrete,while the replacement rate of RCA has a remarkable effect on the long-term deformation of the concrete beams. | Jingwei YING Feiming SU Shuangren CHEN | 2022 | Frontiers of Structural and Civil Engineering2022，16，3： | 0 |
| 12 | Yiqi Qingre Xiaozheng formula protects against diabetic nephropathy by restoring autophagy in mice显示文摘Objective:To evaluate the protective effect of Yiqi Qingre Xiaozheng formula(YQQRXZF)via the regulation of autophagy in diabetic nephropathy(DN)mice induced by injection of streptozotocin(STZ)after high fat diet(HFD).Methods:The composition of YQQRXZF was analyzed by high performance liquid chromatographyelectrospray ionization/mass spectrometry(HPLC-ESI/MSn).The DN model was induced by intraperitoneally injection of 50 mg/kg STZ within 5 days,followed by HFD feeding for 8 weeks in C57BL/6J mice.Mice were randomly separated into DN group,YQQRXZF group,irbesartan group,and control group.Blood glucose was calculated,and body weight was measured every 2 weeks.An enzyme-linked immunosorbent assay was used to measure the urinary albumin-to-creatinine ratio(uACR)before and after treatment,and the serum concentrations of total cholesterol(TC),low-density lipoprotein(LDL),triglycerides(TG),high-density lipoprotein(HDL),blood urea nitrogen(BUN),and serum creatinine(Scr)were measured.In addition,hematoxylin-eosin(H&E)staining,periodic acid-Schiff(PAS)staining,Masson's trichrome staining,and transmission electron microscopy(TEM)were used to observe pathological changes in renal tissue.Autophagy levels were determined by immunofluorescence staining.Results:In this study,21 dominant chemical constituents were identified in YQQRXZF.The treatment group reduced u ACR in a more significant way than the DN group(P=.018).The treatment group reduced TC,TG,and LDL concentrations after YQQRXFF treatment(P=.021,P=.014 and P=.026,respectively).H&E,PAS,and Masson staining showed that pathological damage to mice kidneys improved,the volume of renal glomeruli was reduced,and glomerular sclerosis and fibrosis were reduced.Immunofluorescence analysis revealed that expressions of LC3-Ⅱprotein increased in the treatment group(P=.013).In contrast,the protein expressions of P62 were reduced after treatment(P=.025). | Na Sun Nan Yang Jingwei Zhou Ying He Jian Wang Yingnan Liang Run Dai Jiajia Bai Zhenjie Chen | 2023 | Journal of Traditional Chinese Medical Sciences2023，10，3： | 0 |
| 13 | Chloride diffusion in concrete with carbonated recycled coarse aggregates under biaxial compression显示文摘Chloride attack on concrete structures is affected by the complex stress state inside concrete,and the effect of recycled aggregates renders this process more complex.Enhancing the chloride resistance of recycled concrete in a complex environment via carbonization facilitates the popularization and application of recycled concrete and alleviates the greenhouse effect.In this study,the chloride ion diffusion and deformation properties of recycled concrete after carbonization are investigated using a chloride salt load-coupling device.The results obtained demonstrate that the chloride ion diffusivity of recycled concrete first decreases and then increases as the compressive load increases,which is consistent with the behavior of concrete,in that it first undergoes compressive deformation,followed by crack propagation.Carbonation enhances the performance of the recycled aggregates and reduces their porosity,thereby reducing the chloride diffusion coefficient of the recycled concrete under different compressive load combinations.The variation in the chloride ion diffusivity of the carbonized recycled aggregate concrete with the load is consistent with a theoretical formula. | Jingwei YING Weibeng WANG Jianzhuang XIAO | 2023 | Frontiers of Structural and Civil Engineering2023，17，4： | 0 |
| 14 | α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure显示文摘The sarcomeric interaction ofα-myosin heavy chain(α-MHC)with Titin is vital for cardiac structure and contraction.However,the mechanism regulating this interaction in normal and failing hearts remains unknown.Lactate is a crucial energy substrate of the heart.Here,we identify thatα-MHC undergoes lactylation on lysine 1897 to regulate the interaction ofα-MHC with Titin.We observed a reduction ofα-MHC K1897 lactylation in mice and patients with heart failure.Loss of K1897 lactylation inα-MHC K1897R knock-in mice reducesα-MHC–Titin interaction and leads to impaired cardiac structure and function.Furthermore,we identified that p300 and Sirtuin 1 act as the acyltransferase and delactylase ofα-MHC,respectively.Decreasing lactate production by chemical or genetic manipulation reducesα-MHC lactylation,impairsα-MHC–Titin interaction and worsens heart failure.By contrast,upregulation of the lactate concentration by administering sodium lactate or inhibiting the pivotal lactate transporter in cardiomyocytes can promoteα-MHC K1897 lactylation andα-MHC–Titin interaction,thereby alleviating heart failure.In conclusion,α-MHC lactylation is dynamically regulated and an important determinant of overall cardiac structure and function.Excessive lactate efflux and consumption by cardiomyocytes may decrease the intracellular lactate level,which is the main cause of reducedα-MHC K1897 lactylation during myocardial injury.Our study reveals that cardiac metabolism directly modulates the sarcomeric structure and function through lactate-dependent modification ofα-MHC. | Naijin Zhang Ying Zhang Jiaqi Xu Pengbo Wang Boquan Wu Saien Lu Xinxin Lu Shilong You Xinyue Huang Mohan Li Yuanming Zou Mengke Liu Yuanhui Zhao Guozhe Sun Wenbin Wang Danxi Geng Jingwei Liu Liu Cao Yingxian Sun | 2023 | Cell Research2023，33，9： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员