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| 1 | Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma显示文摘Background:Resistance to ferroptosis,a regulated cell death caused by irondependent excessive accumulation of lipid peroxides,has recently been linked to lung adenocarcinoma(LUAD).Intracellular antioxidant systems are required for protection against ferroptosis.The purpose of the present studywas to investigate whether and how extracellular system desensitizes LUAD cells to ferroptosis.Methods:Established human lung fibroblasts MRC-5,WI38,and human LUAD H1650,PC9,H1975,H358,A549,and H1299 cell lines,tumor and matched normal adjacent tissues of LUAD,and plasma from healthy individuals and LUAD patients were used in this study.Immunohistochemistry and immunoblotting were used to analyze protein expression,and quantitative reverse transcription-PCR was used to analyze mRNA expression.Cell viability,cell death,and the lipid reactive oxygen species generationwere measured to evaluate the responses to ferroptosis.Exosomes were observed using transmission electron microscope.The localization of arachidonic acid(AA)was detected using click chemistry labeling followed by confocal microscopy.Interactions between RNAs and proteins were detected using RNA pull-down,RNA immunoprecipitation and photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation methods.Proteomic analysis was used to investigate RNA-regulated proteins,and metabolomic analysis was performed to analyze metabolites.Cellderived xenograft,patient-derived xenograft,cell-implanted intrapulmonary LUAD mouse models and plasma/tissue specimens from LUAD patients were used to validate the molecular mechanism.Results:Plasma exosome from LUAD patients specifically reduced lipid peroxidation and desensitized LUAD cells to ferroptosis.A potential explanation is that exosomal circRNA_101093(cir93)maintained an elevation in intracellular cir93 in LUAD to modulate AA,a poly-unsaturated fatty acid critical for ferroptosisassociated increased peroxidation in the plasma membrane.Mechanistically,cir93 interacted with and increased fatty acid-binding protein 3(FABP3),which transported AA and facilitated its reaction with taurine.Thus,global AA was reduced,whereas N-arachidonoyl taurine(NAT,the product of AA and taurine)was induced.Notably,the role of NAT in suppressing AA incorporation into the plasma membrane was also revealed.In pre-clinical in vivo models,reducing exosome improved ferroptosis-based treatment.Conclusion:Exosome and cir93 are essential for desensitizing LUAD cells to ferroptosis,and blocking exosome may be helpful for future LUAD treatment. | Xiao Zhang Yunhua Xu Lifang Ma Keke Yu Yongjie Niu Xin Xu Yi Shi Susu Guo Xiangfei Xue Yikun Wang Shiyu Qiu Jiangtao Cui Hong Wang Xiaoting Tian Yayou Miao Fanyu Meng Yongxia Qiao Yongchun Yu Jiayi Wang | 2022 | Cancer Communications2022，42，4： | 10 |
| 2 | The generation and accumulation of natural gas from Yinan 2 gas pool in Kuqa Depression显示文摘By using the methods of hydrocarbon generation kinetics and carbon isotope kinetics, combined with geological background of natural gas pool formation, the generation and accumulation of natural gas from Yinan 2 gas pool was studied in Kuqa Depression of the Tarim Basin. Natural gas of Yinan 2 gas pool is mainly derived from Middle and Lower Jurassic coal-bearing source rocks, and generally belongs to long time-accumulated gas. It is suggested that Yinan 2 gas is chiefly accumulated in the last 5 Ma, its Ro ranges from 1.25% to 1.95%, and the loss rate of natural gas is about 25%-30%. This work not only complements and reduces the deficiency of formation model of natural gas pools which traditionally depends on the matching relationships between source rock, reservoir, cap rock, and trap, but also is a useful reference in the study of other gas pools. | LI Xianqing XIAO Xianming TANG Yongchun XIAO Zhongyao Ml Jingkui LIU Dehan SHEN Jiagui LIU Jinzhong | 2004 | Chinese Science Bulletin2004，49，S1： | 6 |
| 3 | Secondary aerosol formation and oxidation capacity in photooxidation in the presence of Al_2O_3 seed particles and SO_2显示文摘To investigate the sensitivity of secondary aerosol formation and oxidation capacity to NOx in homogeneous and heterogeneous reactions, a series of irradiated toluene/NOx/air and ?-pinene/NOx/air experiments were conducted in smog chambers in the absence or presence of Al2O3 seed particles. Various concentrations of NOx and volatile organic compounds(VOCs) were designed to simulate secondary aerosol formation under different scenarios for NOx. Under 'VOC-limited' conditions, the increasing NOx concentration suppressed secondary aerosol formation, while the increasing toluene concentration not only contributed to the increase in secondary aerosol formation, but also led to the elevated oxidation degree for the organic aerosol. Sulfate formation was suppressed with the increasing NOx due to a decreased oxidation capacity of the photooxidation system. Secondary organic aerosol(SOA) formation also decreased with the presence of high concentration of NOx, because organo-peroxy radicals(RO2) react with NOx instead of with peroxy radicals(RO2 or HO2), resulting in the formation of volatile organic products. The increasing concentration of NOx enhanced the formation of sulfate, nitrate and SOA under 'NOx-limited' conditions, in which the heterogeneous reactions played an important role. In the presence of Al2O3 seed particles, a synergetic promoting effect of mineral dust and NOx on secondary aerosol formation in heterogeneous reactions was observed in the photooxidation. This synergetic effect strengthened the positive relationship between NOx and secondary aerosol formation under 'NOx-limited' conditions but weakened or even overturned the negative relationship between NOx and secondary aerosol formation under 'VOC-limited' conditions. Sensitivity of secondary aerosol formation to NOx seemed different in homogeneous and heterogeneous reactions, and should be both taken into account in the sensitivity study. The sensitivity of secondary aerosol formation to NOx was further investigated under 'winter-like' and NH3-rich conditions. No obvious difference for the sensitivity of secondary aerosol formation except nitrate to NOx was observed. | Biwu Chu Tengyu Liu Xiao Zhang Yongchun Liu Qingxin Ma Jinzhu Ma Hong He Xinming Wang Junhua Li Jiming Hao | 2015 | Science China Chemistry2015，58，9： | 3 |
| 4 | Vesicle-shaped ZIF-8 shell shielded in 3D carbon cloth for uniform nucleation and growth towards long-life lithium metal anode显示文摘Lithium metal has aroused extensive research interests as the anode for next-generation rechargeable batteries.However,the well-known dendritic Li growth and consequent safety issues still impair the long-term cycling performance.Herein,a hybrid structure composed of 3 D carbon cloth and vesicleshaped hollow ZIF-8 modification shell(HZS@CC)was prepared as a smart host for guiding uniform Li deposition.The long-range interconnected 3 D carbon fiber network enables the reduced local current density with homogeneous electrons distribution.Synergistically,abundant surface polar groups and the ultrastructure on ZIF-8 particles effectively guide a well-distributed Li-ions flow to promote the uniform Li nucleation and growth.As a result,stable Li plating/stripping for 2000 h with a low overpotential(≈15 mV)at 1 mA cm^(-2) were achieved in symmetric cells.Coupling with LiFePO_(4) cathode,the full cell delivered long life over 1200 cycles at 6 C.This research demonstrated that a homogenization guiding of Li-ions is of great importance to better make use of the structural advantage of 3 D hosts and achieve improved electrochemical performance. | Yuting Fang Wenlong Cai Shanshan Zhu Kangli Xu Maogen Zhu Guannan Xiao Yongchun Zhu | 2021 | Journal of Energy Chemistry2021，30，3： | 2 |
| 5 | Kinetic Parameters of Methane Generated from Source Rocks in the Kuqa Depression of Tarim Basin and Their Application显示文摘摘要:在金试管的一个热模拟实验靠近系统,用 KINETICS 和 GOR 同位素动力学软件,煤气的产生的运动参数和从 TriassicJurassic 烃来源的同位素的分别在 Tarim 盆的 Kuqa 消沉摇的甲烷碳计算被获得。分别地,在 Kuqa 消沉从侏罗记煤,侏罗记 mudstone 和三叠纪的 mudstone 产生的甲烷的激活精力是 197268 kJ/mol, 180260 kJ/mol 和 214289 kJ/mol,他们的频率因素是 5.265times1013s ? 1, 9.761times1011s ? 1 并且 2.270times1014s ? 1。这反映他们烃产生行为的差别。甲烷碳的运动参数同位素的分别在侏罗记煤,侏罗记 mudstone 和三叠纪的 mudstone 也是不同的,谁的平均激活精力,分别地是 228 kJ/mol, 205 kJ/mol 和 231 kJ/mol。与地质的背景结合了,在 Yinan-2 煤气的水池的天然气的起源被讨论,并且天然气的一个累积模型因此被建立。Yinan-2 气体首先在 Yangxia 从侏罗记忍受煤的来源岩石被导出下垂。主要煤气的累积时间是 50 妈,相应 Ro 在从 1.25%1.95% 的范围。天然气的损失率是 25%30% 。 | LI Xianqing XIAO Xianming MI Jingkui TANG Yongchun XIAO Zhongyao LIU Dehan SHEN Jiagui YANG Yunfeng WANG Yan DONG Peng | 2008 | Acta Geologica Sinica(English Edition)2008，82，1： | 2 |
| 6 | Tunable optical second-order sideband effects in a parity-time symmetric optomechanical system显示文摘We theoretically investigate the optical second-order sideband generation(OSSG)in an optical parity-time(PT)symmetric system,which consists of a passive cavity trapping the atomic ensemble and an active cavity.Compared with the double-passive system,it is found that near the exceptional point(EP),the efficiency of the OSSG increases sharply not only for the blue probepump detuning resonant case but also for the red one.Using experimentally achievable parameters,we study the effect of the atomic ensemble on the efficiency of the OSSG in the PT-symmetric system.The numerical results show that the efficiency of the OSSG is 30%higher than that of the first-order sideband,which is realized easily by simultaneously modulating the atom-cavity coupling strength and detuning.Moreover,the efficiency of the OSSG can also be tuned effectively by the pump power,and the efficiency is robust when the pump power is strong enough.This study may have some guidance for modulating the nonlinear optical properties and controlling light propagation,which may stimulate further applications in optical communications. | Xing Xiao QingHong Liao NanRun Zhou WenJie Nie YongChun Liu | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，11： | 1 |
| 7 | Growth modulation of simultaneous epitaxy of ZnO obliquely aligned nanowire arrays and film on r-plane sapphire substrate显示文摘底层上的电影和 nanowire 数组的同时的取向附生的生长为 nanoscale 光电子具有科学意义和实际重要性。不过,在造的 situ 进行在在绝缘的底层上种的个别地孤立的 nanowires 之间的连接仍然是挑战性的。此处,我们在 Au 涂的 r 飞机蓝宝石底层上为 nonpolar 一飞机 ZnO 电影和倾斜地排列的 nanowire 数组的同时的取向附生的生长表明新奇、灵巧的策略。同样综合的 ZnO nanostructures 的形态学,结构,部件,和光性质用扫描电子显微镜学, X 光检查衍射,地排放传播电子显微镜学,精力散的光谱学, X 光检查光电子光谱学,和光致发光的地排放被调查光谱学。合作生长机制被建议: 催化Au 的蒸汽运输开始 nonpolar 一飞机和极的c飞机 ZnO 原子核的同现,并且随后,极的c飞机 ZnO 原子核同时开发进倾斜地排列的 nanowire 数组的 nonpolar 一飞机 ZnO 原子核在自我催化的 vapor-liquid-solid 生长帮助的底部和锌开发进一部 ZnO 传导性的电影的非向上的指导 Au 催化剂帮助。与传导性的连接和它在 nanowires 的 situ 合成认识到的建议策略能在光电子有益于 ZnO nanowires 的应用程序。 | Yongchun Xiao Yaoyao Tian Shujing sun Chenlong Chen Buguo Wang | 2018 | Nano Research2018，11，7： | 1 |
| 8 | An experimental comparison of gas generation from three oil fractions: Implications for the chemical and stable carbon isotopic signatures of oil cracking gas显示文摘 | Hui Tian Xianming Xiao Ronald W.T. Wilkins Yongchun Tang | 2012 | Organic Geochemistry2012，，： | 1 |
| 9 | TFCP2蛋白为YAP发挥转录依赖性促肝癌效应所必需显示文摘文章简介虽然促癌蛋白YAP依赖性转录与肝癌发生发展密切相关,但YAP维持其促癌功能的分子机制尚未充分阐明。本研究证明TFCP2是YAP依赖性转录促肝癌效应的必需核因子。 | Xiao Zhang Fenyong Sun Yongxia Qiao Weisheng Zheng Ya Liu Yan Chen Qi Wu Xiangfan Liu Guoqing Zhu Yuxin Chen Yongchun Yu 潘秋辉 王佳谊 | 2018 | 科学新闻2018，0，4： | 0 |
| 10 | Rapid and sensitive detection of cucumber mosaic virus by reverse transcription loop-mediated isothermal amplification显示文摘Cucumber mosaic virus(CMV),as a member of Cucumovirus genus,is one of the most common lily viruses and has been reported in many countries [1,2].The CMV-infected plants may show developmental abnormalities of leaves,flowers,and bulbs [1].The symptoms will be extremely severe in plants infected by lily symptomless virus(LSV)[1].So far,CMV has posed a threat to cultivars of lily production.Consequently,it is important to develop an efficient approach to achieve rapid and sensitive detection of CMV.Many visual and serological methods have been reported to detect CMV,such as double antibody sandwich enzyme-linked immunosorbent assay [1],immunosorbent electron microscopy [1],enzyme-linked immunosorbent assay(ELISA)and reverse transcriptase-polymerase chain reaction(RT-PCR)[2].However,these methods are time-consuming and require skilled operator and specialized equipment.Loop-mediated isothermal amplification(LAMP)method provides a possible solution for these problems [3].LAMP was developed by Notomi et al.[4] with high specificity and sensitivity because of Bst DNA polymerase.LAMP method not only is suitable for field and large-scale detection of animal virus with more cost-effective but also could be applied to detect foodborne bacterial pathogens and other viruses with high performances [5].For a naked-eye inspection,SYBR Green I was added to the LAMP reaction products to visualize the results.In this study,we developed a rapid and sensitive LAMP method to detect CMV in lily plants and a simple reverse transcription loop-mediated isothermal amplification(RT-LAMP)to detect field CMV samples in lily bulbs. | Xiaorui Fan Yanan Du Youming Cai Yongchun Zhang Xiao Zhao Jieling Liang Dan Yang Qi Zhang Xiaoxia Zhang Wanjing Zhang Yan Xu Kai Zhao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 0 |
| 11 | The generation kinetics of natural gases in the Kela-2 gas field from the Kuqa Depression, Tarim Basin, northwestern China显示文摘The Kela-2 gas field, found in the Kuqa Depression of the Tarim Basin, northwestern China, is a large-sized dry gas field (C1 /C1-5 =0.992 0.999) and characterized by ultra-high pressure (pressure factor up to 2.0 2.2). The pyrolysis experiment was carried out under isothermal gold-tube closed system, with samples collected from the Jurassic coal, Jurassic mudstone and Triassic mudstone in the Kuqa Depression. The result of gas yield showed that the Middle and Lower Jurassic source rocks have higher gas generation potential than the Triassic source rocks. The kinetic modeling of gas generation and methane carbon isotope fractionation suggested that the Kela-2 gases belong to the products of high-over mature stages and were mainly derived from the Middle and Lower Jurassic coal-bearing strata. The Triassic source rocks made a minor contribution to the Kela-2 gases. The Kela-2 gases chiefly generated from coal-bearing source rocks with R o values from 1.3% to 2.5%, and thus primarily accumulated after 5 Ma. | LI Xianqing FENG Songbao XIAO Xianming TANG Yongchun XIAO Zhongyao MI Jingkui TIAN Hui LIU Dehan SHEN Jiagui | 2013 | Chinese Journal Of Geochemistry2013，32，2： | 0 |
| 12 | CASCIRE surveillance network and work on avian influenza viruses显示文摘Studies on influenza virus by Chinese Academy of Sciences(CAS)could be traced back as early as 2005 by the CAS Key Laboratory of Pathogenic Microbiology and Immunology(CASPMI),who discovered that Qinghai-like Clade 2.2H5N1 subtype highly pathogenic avian influenza virus(HPAIV)first caused severe outbreak in wild birds in Qinghai Lake(Liu et al.,2005). | Yuhai Bi Weifeng Shi Jianjun Chen Quanjiao Chen Zhenghai Ma Gary Wong Wenxia Tian Renfu Yin Guanghua Fu Yongchun Yang William J.Liu Chuansong Quan Qianli Wang Shenghu He Xiangdong Li Qianfeng Xia Lixin Wang Zhaohui Pan Laixing Li Hong Li Wen Xu Ying Luo Hui Zeng Lianpan Dai Haixia Xiao Kirill Sharshov Alexander Shestopalov Yi Shi Jinghua Yan Xuebing Li Yingxia Liu Fumin Lei Wenjun Liu George F.Gao | 2017 | Science China(Life Sciences)2017，60，12： | 0 |
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