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| 1 | Maize Genic Male-Sterility Genes and Their Applications in Hybrid Breeding: Progress and Perspectives显示文摘As one of the most important crops, maize not only has been a source of the food, feed, and industrial feedstock for biofuel and bioproducts, but also became a model plant system for addressing fundamental questions in genetics. Male sterility is a very useful trait for hybrid vigor utilization and hybrid seed production. The identification and characterization of genic male-sterility (GMS) genes in maize and other plants have deepened our understanding of the molecular mechanisms controlling anther and pollen development, and enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for crop hybrid breeding. In this review, we summarize main advances on the identification and characterization of GMS genes in maize, and con struct a putative regulatory network controlling maize anther and pollen development by comparative genomic analysis of GMS genes in maize, Arabidopsis, and rice. Furthermore, we discuss and appraise the features of more than a dozen BMS systems for propagating male-sterile lines and producing hybrid seeds in maize and other plants. Finally, we provide our perspectives on the studies of GMS genes and the development of novel BMS systems in maize and other plants. The continuous exploration of GMS genes and BMS systems will enhance our understanding of molecular regulatory networks controlling male fertility and greatly facilitate hybrid vigor utilization in breeding and field production of maize and other crops. | Xiangyuan Wan Suowei Wu Ziwen Li Zhenying Dong Xueli An Biao Ma Youhui Tian Jinping Li | 2019 | Molecular Plant2019，12，3： | 24 |
| 2 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 3 | Lipid Metabolism: Critical Roles in Male Fertility and Other Aspects of Reproductive Development in Plants显示文摘Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation.Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility(GMS).To date,many lipid metabolism-related GMS genes that are involved in the formation of anther cuticle,pollen wall,and subcellular organelle membranes in anther wall layers have been identified and characterized.In this review,we summarize recent progress on characterizing lipid metabolism-related genes and their roles in male fertility and other aspects of reproductive development in plants.On the basis of cloned GMS genes controlling biosynthesis and transport of anther cutin,wax,sporopollenin,and tryphine\r\Arabidopsis,rice,and maize as well as other plant species,updated lipid metabolic networks underlying anther cuticle development and pollen wall formation were proposed.Through bioinformatics analysis of anther RNA-sequencing datasets from three maize inbred lines(Oh43,W23,and B73),a total of 125 novel lipid metabolism-related genes putatively involved in male fertility in maize were deduced.More,we discuss the pathways regulating lipid metabolism-related GMS genes at the transcriptional and post-transcriptional levels.Finally,we highlight recent findings on lipid metabolism-related genes and their roles in other aspects of plant reproductive development.A comprehensive understanding of lipid metabolism,genes involved,and their roles in plant reproductive development will facilitate the application of lipid metabolism-related genes in gene editing,haploid and callus induction,molecular breeding and hybrid seed production in crops. | Xiangyuan Wan Suowei Wu Ziwen Li Xueli An Youhui Tian | 2020 | Molecular Plant2020，13，7： | 15 |
| 4 | Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene显示文摘Maize(Zea mays L.)is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013(Baldaufa et al.,2016).Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s(Duvick,2001).The high-yield maize varieties used today are all hybrids,and the production of hybrid maize seed requires sterilization to prevent self-pollination.Conventional seed produc- | Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 5 | A New Species of the Genus Tylototriton(Amphibia,Salamandridae) from Hunan,China显示文摘A new species of the genus Tylototriton is described in this article based on morphological and genetic evidence. The new species is identified belonging to the T. asperrimus group and is similar to T. wenxianensis. The diagnostic characters of the new species are as follows: dorsal ridge broad and thick,its width approximately equal to eye diameter(P < 0.001); tail height greater than width at base of tail(P < 0.001); no villous genital papilla found inside the male anal fissure; nodule-like warts,along lateral margin of the trunk,bulge and forming tubercles,and thin and transverse striae present between the tubercles. | Youhui SHEN Jianping JIANG Xiaoyang MO | 2012 | Asian Herpetological Research2012，3，1： | 7 |
| 6 | A new species of Megophrys (Amphibia:Anura:Megophryidae) from the northwestern Hunan Province,China显示文摘A new species of Megophrys (Amphibia: Anura: Megophryidae), Megophrys tuberogranulatus Shen, Mo et Li, sp.nov. (Holotype HNUL 03080902, adult male, SVL: 38.2 mm), from Sangzhi County, Hunan Province, is described. Megophrystuberogranulatus is closely related to both Megophrys kuatunensis and Megophrys minor. Characters of the new species that differentiatethis species from close relatives are skin granulated with big tubercles on the dorsal sides of both thigh and tibia, thebigger inner metacarpal tubercle, upper eyelid wider than the interorbital space, hind limbs longer, overlapping of the left andright calcaneal parts. The type specimens are deposited in the Animal Museum of Life Sciences College of Hunan Normal University. | Xiaoyang MO Youhui SHEN Honghui LI Xiushan WU | 2010 | Current Zoology2010，56，4： | 6 |
| 7 | A New Species of the Genus Tylototriton (Urodela: Salamandridae) from Northeastern Hunan Province, China显示文摘We describe a new species from the family Salamandridae from northeastern Hunan Province of China based on morphological and molecular genetic evidences.The new species,Tylototriton liuyangensis,is a member of the T.asperrimus group,and shares a number of similarities with T.wenxianensis and T.broadoridgus.The dorsal surface of the new Tylototriton species is completely black,with nodule-like warts distributed evenly along the lateral margin of dorsal body,and there is no transverse striae between the warts.There are several diagnostic characters of the new species that differ from those of T.wenxianensis and T.broadoridgus as follows:(1)the interorbital space(IOS)is comparatively wide;(2)there is a bony ridge present on the dorsal head surface,running along the upper eyelids;(3)the space between axilla and groin(AGS)is comparatively large,with the mean male AGS approximately equal to 50%of the snout-vent length(SVL)and the mean female AGS being 53%of SVL;and(4)there is a small genital armature on the anterior angle of labial folds present inside the anal fissure of adult males during the breeding season.The result from the molecular phylogenetic analysis of the genus Tylototriton(including the type specimen of the new species)based on the complete DNA sequence of the mitochondrial ND2 gene(1035 bp)indicated that the new species was similar to T.wenxianensis,T.dabienicus,and T.broadoridgus,but formed an independent clade.This result was consistent with the morphological analysis above,supporting that the Liuyang Population represented a distinct species. | Daode YANG Jianping JIANG Youhui SHEN Dongbo FEI | 2014 | Asian Herpetological Research2014，5，1： | 5 |
| 8 | Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells显示文摘The restriction of immunoglobulin(Ig)expression to B lymphocytes is well established.However,several reports have confirmed that the Ig gene can be expressed in many non-B cancer cells and/or some normal cells.Our aim is to determine whether the Ig gene promoter can be activated in non-B cancer cells and to identify the regulatory mechanism for Ig gene expression.Our results show that the Ig promoter of VH4-59 was activated in several non-B cancer cell lines.Moreover,two novel positive regulatory elements,an enhancer-like element at 2800 to 2610 bp and a copromoter-like element at 2610 to 2300 bp,were identified in two epithelial cancer cell lines,HeLa S3 and HT-29.The octamer element(59-ATGCAAAT-39)located in the Ig promoter,a crucial element for B-cell-derived Ig gene transcription,was also very important for non-B-cell-derived Ig gene transcription.More importantly,we confirmed that octamer-related protein-1(Oct-1),but not Oct-2,was a crucial transcriptional factor for Ig gene transcription due to its ability to bind to the octamer element of the Ig promoter in epithelial cancer cells.These results suggested the presence of a distinct regulatory mechanism for Ig gene expression in non-B cancer cells. | Xiaohui Zhu Lina Wu Li Zhang Peng Hao Shuai Zhang Jing Huang Jie Zheng Yinan Liu Wenjun Li Yingmei Zhang Chunyan Zhou Youhui Zhang C Cameron Yin Xiaoyan Qiu | 2010 | Cellular & Molecular Immunology2010，7，4： | 5 |
| 9 | MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B显示文摘Pathological cardiac hypertrophy serves as a significant foundation for cardiac dysfunction and heart failure. Recently, growing evidence has revealed that microRNAs(miRNAs) play multiple roles in biological processes and participate in cardiovascular diseases. In the present research, we investigate the impact of miRNA-34 c-5 p on cardiac hypertrophy and the mechanism involved. The expression of miR-34 c-5 p was proved to be elevated in heart tissues from isoprenaline(ISO)-infused mice. ISO also promoted miR-34 c-5 p level in primary cultures of neonatal rat cardiomyocytes(NRCMs). Transfection with miR-34 c-5 p mimic enhanced cell surface area and expression levels of foetal-type genes atrial natriuretic factor(Anf) and β-myosin heavy chain(β-Mhc) in NRCMs. In contrast, treatment with miR-34 c-5 p inhibitor attenuated ISO-induced hypertrophic responses. Enforced expression of miR-34 c-5 p by tail intravenous injection of its agomir led to cardiac dysfunction and hypertrophy in mice, whereas inhibiting miR-34 c-5 p by specific antagomir could protect the animals against ISO-triggered hypertrophic abnormalities. Mechanistically, miR-34 c-5 p suppressed autophagic flux in cardiomyocytes, which contributed to the development of hypertrophy. Furthermore, the autophagy-related gene 4 B(ATG4 B) was identified as a direct target of miR-34 c-5 p, and miR-34 c-5 p was certified to interact with 3’untranslated region of Atg4 b mRNA by dual-luciferase reporter assay. miR-34 c-5 p reduced the expression of ATG4 B, thereby resulting in decreased autophagy activity and induction of hypertrophy. Inhibition of miR-34 c-5 p abolished the detrimental effects of ISO by restoring ATG4 B and increasing autophagy. In conclusion, our findings illuminate that miR-34 c-5 p participates in ISO-induced cardiac hypertrophy, at least partly through suppressing ATG4 B and autophagy. It suggests that regulation of miR-34 c-5 p may offer a new way for handling hypertrophy-related cardiac dysfunction. | Yuhong Zhang Yanqing Ding Min Li Jing Yuan Youhui Yu Xueying Bi Huiqi Hong Jiantao Ye Peiqing Liu | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 4 |
| 10 | International Freight Forwarding Services Network in the Yangtze River Delta, 2005–2015: Patterns and Mechanisms显示文摘This study examined the spatio-temporal trajectories of the international freight forwarding service(IFFS) in the Yangtze River Delta(YRD) and explored the driving mechanisms of the service. Based on a bipartite network projection from an IFFS firm-city data source, we mapped three IFFS networks in the YRD in 2005, 2010, and 2015. A range of statistical indicators were used to explore changes in the spatial patterns of the three networks. The underlying influence of marketization, globalization, decentralization, and integration was then explored. It was found that the connections between Shanghai and other nodal cities formed the backbones of these networks. The effects of a city's administrative level and provincial administrative borders were generally obvious. We found several specific spatial patterns associated with IFFS. For example, the four non-administrative centers of Ningbo, Suzhou, Lianyungang, and Nantong were the most connected cities and played the role of gateway cities. Furthermore, remarkable regional equalities were found regarding a city's IFFS network provision, with notable examples in the weakly connected areas of northern Jiangsu and southwestern Zhejiang. Finally, an analysis of the driving mechanisms demonstrated that IFFS network changes were highly sensitive to the influences of marketization and globalization, while regional integration played a lesser role in driving changes in IFFS networks. | LIANG Shuangbo CAO Youhui WU Wei GAO Jinlong LIU Weichen ZHANG Weiyang | 2019 | Chinese Geographical Science2019，29，1： | 4 |
| 11 | Tailoring the Meso-Structure of Gold Nanoparticles in Keratin-Based Activated Carbon Toward High-Performance Flexible Sensor显示文摘Flexible biosensors with high accuracy and reliable operation in detecting pH and uric acid levels in body fluids are fabricated using well-engineered metaldoped porous carbon as electrode material.The gold nanoparticles@N-doped carbon in situ are prepared using wool keratin as both a novel carbon precursor and a stabilizer.The conducting electrode material is fabricated at 500℃ under customized parameters,which mimics A-B type(two different repeating units) polymeric material and displays excellent deprotonation performance(pH sensitivity).The obtained pH sensor exhibits high pH sensitivity of 57 mV/pH unit and insignificant relative standard deviation of 0.088%.Conversely,the composite carbon material with sp^2 structure prepared at 700℃ is doped with nitrogen and gold nanoparticles,which exhibits good conductivity and electrocatalytic activity for uric acid oxidation.The uric acid sensor has linear response over a range of 1-150 μM and a limit of detection 0.1 μM.These results will provide new avenues where biological material will be the best start,which can be useful to target contradictory applications through molecular engineering at mesoscale. | Aniruddha BPatil Zhaohui Meng Ronghui Wu Liyun Ma Zijie Xu Chenyang Shi Wu Qiu Qiang Liu Yifan Zhang Youhui Lin Naibo Lin Xiang Yang Liu | 2020 | Nano-Micro Letters2020，12，9： | 3 |
| 12 | Gene expression profiles of the Southern house mosquito Culex quinquefasciatus during exposure to permethrin显示文摘 | William R. Reid Lee Zhang Youhui Gong Ting Li Nannan Liu | 2018 | Insect Science2018，25，3： | 3 |
| 13 | Performance of common buckwheat(Fagopyrum esculentum M.) supplied with selenite or selenate for selenium biofortification in northeastern China显示文摘Selenium(Se)deficiency commonly occurs in soils of northeastern China and leads to insufficient Se intake by humans.A two-year field study of Se biofortification of common buckwheat supplied with 40 g Se ha^(-1)as selenite(Se(IV)),selenate(Se(VI)),or a combination(1/2 Se(IV+VI))was performed to investigate Se accumulation and translocation in plants and determine the effects of different forms of Se on the grain yield,biomass production,and Se use efficiency of plants and seeds.Se application increased seed Se concentrations to 47.1–265.1μg kg^(-1).Seed Se concentrations following Se(VI)or 1/2 Se(IV+VI)treatment exceeded 100μg kg^(-1),an amount suitable for crop Se biofortification.Se concentration in shoots and roots decreased with plant development,and Se translocation from root to shoot in Se(IV)-treated plants was lower than that in plants treated with 1/2 Se(IV+VI)and Se(VI).Both grain yield and biomass production increased under 1/2 Se(IV+VI)treatment,with grain yields reaching 1663.8 and 1558.5 kg ha^(-1)in 2015 and 2016,respectively,reflecting increases of 11.0% and 10.3% over those without Se application.The Se use efficiency of seeds and plants under Se(VI)treatment was significantly higher than those under 1/2 Se(IV+VI)and Se(IV)treatments.Thus,application of selenate could result in higher Se accumulation in buckwheat seeds than application of the other Se sources,but the combined application of selenate and selenite might be an alternative approach for improving buckwheat grain yield by Se biofortification in northeastern China. | Ying Jiang Xiaomin Feng Yadong Yang Xiangxue Qi Yongfeng Ren Youhui Gao Weidong Liu Yuegao Hu Zhaohai Zeng | 2018 | The Crop Journal2018，6，4： | 3 |
| 14 | XB-SIM*:A Simulation Framework for Modeling and Exploration of ReRAM-Based CNN Acceleration Design显示文摘Resistive Random Access Memory(ReRAM)-based neural network accelerators have potential to surpass their digital counterparts in computational efficiency and performance.However,design of these accelerators faces a number of challenges including imperfections of the Re RAM device and a large amount of calculations required to accurately simulate the former.We present XB-SIM,a simulation framework for Re RAM-crossbar-based Convolutional Neural Network(CNN)accelerators.XB-SIM can be flexibly configured to simulate the accelerator’s structure and clock-driven behaviors at the architecture level.This framework also includes an Re RAM-aware Neural Network(NN)training algorithm and a CNN-oriented mapper to train an NN and map it onto the simulated design efficiently.Behavior of the simulator has been verified by the corresponding circuit simulation of a real chip.Furthermore,a batch processing mode of the massive calculations that are required to mimic the behavior of Re RAM-crossbar circuits is proposed to fully apply the computational concurrency of the mapping strategy.On CPU/GPGPU,this batch processing mode can improve the simulation speed by up to 5.02 or 34.29.Within this framework,comprehensive architectural exploration and end-to-end evaluation have been achieved,which provide some insights for systemic optimization. | Xiang Fei Youhui Zhang Weimin Zheng | 2021 | Tsinghua Science and Technology2021，26，3： | 2 |
| 15 | Effects of Magnetic Annealing on Magnetic Properties in Nd_4Fe_(76)Co_3(Hf_(1-x)Ga_x)B_(16) Alloy显示文摘The best linear fit of H_c(T)/M,(T) vs 2k_1(T)/μ_0M s^2(T) infers that H_c is determined by a nucleation process in nanocrystalline two-phase magnet.The condition of the grain shapes is improved after adding Hf and Ga,but the surfaces are deteriorated by some precipitates on the boundary.Taking into account the values of α,N_(eff),the results of TEM,and Moessbauer spectroscopy,the magnetic field heat-treatment not only induces grain refinement but also causes a uniform distribution of the soft and hard phases.It is one recommendable method to improve the condition of the microstructure.Both the remanence and energy product values are increased by 20%— 30%for all ribbons after annealing with magnetic field.The magnetic interaction in Nd_2Fe_(14)B/Fe_3B + αFe based nanocrystalline two-phase magnets is studied using δM plots in this paper.It is found that the exchange coupled interaction is greatly enhanced in the sample annealed with magnetic heat-treatment,specially,Nd_4Fe_(76)Co_3Hf_(0.5)Ga_(0.5)B_(16) which achieves the highest energy product(BH)_(max)=126.4kJ/m^3. | Gao Youhui Zhu Jinghan Weng Yuqing Eon Byung Park Choong Jin Yang | 1999 | Journal of Iron and Steel Research(International)1999，6，1： | 2 |
| 16 | Effect of γ-ir- radiation on some physioehemieal properties of konjac glucoman- nan显示文摘 | Xu Zhenlin Sun Yuanming Yang Youhui | 2007 | Carbohydrate Polymers2007，70，4： | 1 |
| 17 | Prediction of Transient Responses of a Folding Wing during the Morphing Process显示文摘 | Zhao Youhui Hu Haiyan | 2013 | Aerospace Science and Technology2013，24，1： | 1 |
| 18 | Triple positive solutions of m - point BVPs for p - Laplacian dynamic equations on time scales显示文摘 | Su Youhui Li Wantong | 2008 | Nonlinear Anal2008，69，： | 1 |
| 19 | Automatic software deployment using user-level virtualization for cloud-eompu ting显示文摘 | Zhang Youhui LI Yanhua Zheng Weimin | 2011 | Future Generation Computer Systems2011，，8： | 1 |
| 20 | Periodic solution of second - or- der Hamiltonian systems with a change sign potential on time scales显示文摘 | Su Youhui Li Wantong | 2009 | Discrete Dynamics in Nature and Socie- ty2009，13，： | 1 |
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