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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene显示文摘Maize(Zea mays L.)is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013(Baldaufa et al.,2016).Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s(Duvick,2001).The high-yield maize varieties used today are all hybrids,and the production of hybrid maize seed requires sterilization to prevent self-pollination.Conventional seed produc- | Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 3 | Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells显示文摘The restriction of immunoglobulin(Ig)expression to B lymphocytes is well established.However,several reports have confirmed that the Ig gene can be expressed in many non-B cancer cells and/or some normal cells.Our aim is to determine whether the Ig gene promoter can be activated in non-B cancer cells and to identify the regulatory mechanism for Ig gene expression.Our results show that the Ig promoter of VH4-59 was activated in several non-B cancer cell lines.Moreover,two novel positive regulatory elements,an enhancer-like element at 2800 to 2610 bp and a copromoter-like element at 2610 to 2300 bp,were identified in two epithelial cancer cell lines,HeLa S3 and HT-29.The octamer element(59-ATGCAAAT-39)located in the Ig promoter,a crucial element for B-cell-derived Ig gene transcription,was also very important for non-B-cell-derived Ig gene transcription.More importantly,we confirmed that octamer-related protein-1(Oct-1),but not Oct-2,was a crucial transcriptional factor for Ig gene transcription due to its ability to bind to the octamer element of the Ig promoter in epithelial cancer cells.These results suggested the presence of a distinct regulatory mechanism for Ig gene expression in non-B cancer cells. | Xiaohui Zhu Lina Wu Li Zhang Peng Hao Shuai Zhang Jing Huang Jie Zheng Yinan Liu Wenjun Li Yingmei Zhang Chunyan Zhou Youhui Zhang C Cameron Yin Xiaoyan Qiu | 2010 | Cellular & Molecular Immunology2010，7，4： | 5 |
| 4 | MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B显示文摘Pathological cardiac hypertrophy serves as a significant foundation for cardiac dysfunction and heart failure. Recently, growing evidence has revealed that microRNAs(miRNAs) play multiple roles in biological processes and participate in cardiovascular diseases. In the present research, we investigate the impact of miRNA-34 c-5 p on cardiac hypertrophy and the mechanism involved. The expression of miR-34 c-5 p was proved to be elevated in heart tissues from isoprenaline(ISO)-infused mice. ISO also promoted miR-34 c-5 p level in primary cultures of neonatal rat cardiomyocytes(NRCMs). Transfection with miR-34 c-5 p mimic enhanced cell surface area and expression levels of foetal-type genes atrial natriuretic factor(Anf) and β-myosin heavy chain(β-Mhc) in NRCMs. In contrast, treatment with miR-34 c-5 p inhibitor attenuated ISO-induced hypertrophic responses. Enforced expression of miR-34 c-5 p by tail intravenous injection of its agomir led to cardiac dysfunction and hypertrophy in mice, whereas inhibiting miR-34 c-5 p by specific antagomir could protect the animals against ISO-triggered hypertrophic abnormalities. Mechanistically, miR-34 c-5 p suppressed autophagic flux in cardiomyocytes, which contributed to the development of hypertrophy. Furthermore, the autophagy-related gene 4 B(ATG4 B) was identified as a direct target of miR-34 c-5 p, and miR-34 c-5 p was certified to interact with 3’untranslated region of Atg4 b mRNA by dual-luciferase reporter assay. miR-34 c-5 p reduced the expression of ATG4 B, thereby resulting in decreased autophagy activity and induction of hypertrophy. Inhibition of miR-34 c-5 p abolished the detrimental effects of ISO by restoring ATG4 B and increasing autophagy. In conclusion, our findings illuminate that miR-34 c-5 p participates in ISO-induced cardiac hypertrophy, at least partly through suppressing ATG4 B and autophagy. It suggests that regulation of miR-34 c-5 p may offer a new way for handling hypertrophy-related cardiac dysfunction. | Yuhong Zhang Yanqing Ding Min Li Jing Yuan Youhui Yu Xueying Bi Huiqi Hong Jiantao Ye Peiqing Liu | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 4 |
| 5 | International Freight Forwarding Services Network in the Yangtze River Delta, 2005–2015: Patterns and Mechanisms显示文摘This study examined the spatio-temporal trajectories of the international freight forwarding service(IFFS) in the Yangtze River Delta(YRD) and explored the driving mechanisms of the service. Based on a bipartite network projection from an IFFS firm-city data source, we mapped three IFFS networks in the YRD in 2005, 2010, and 2015. A range of statistical indicators were used to explore changes in the spatial patterns of the three networks. The underlying influence of marketization, globalization, decentralization, and integration was then explored. It was found that the connections between Shanghai and other nodal cities formed the backbones of these networks. The effects of a city's administrative level and provincial administrative borders were generally obvious. We found several specific spatial patterns associated with IFFS. For example, the four non-administrative centers of Ningbo, Suzhou, Lianyungang, and Nantong were the most connected cities and played the role of gateway cities. Furthermore, remarkable regional equalities were found regarding a city's IFFS network provision, with notable examples in the weakly connected areas of northern Jiangsu and southwestern Zhejiang. Finally, an analysis of the driving mechanisms demonstrated that IFFS network changes were highly sensitive to the influences of marketization and globalization, while regional integration played a lesser role in driving changes in IFFS networks. | LIANG Shuangbo CAO Youhui WU Wei GAO Jinlong LIU Weichen ZHANG Weiyang | 2019 | Chinese Geographical Science2019，29，1： | 4 |
| 6 | Tailoring the Meso-Structure of Gold Nanoparticles in Keratin-Based Activated Carbon Toward High-Performance Flexible Sensor显示文摘Flexible biosensors with high accuracy and reliable operation in detecting pH and uric acid levels in body fluids are fabricated using well-engineered metaldoped porous carbon as electrode material.The gold nanoparticles@N-doped carbon in situ are prepared using wool keratin as both a novel carbon precursor and a stabilizer.The conducting electrode material is fabricated at 500℃ under customized parameters,which mimics A-B type(two different repeating units) polymeric material and displays excellent deprotonation performance(pH sensitivity).The obtained pH sensor exhibits high pH sensitivity of 57 mV/pH unit and insignificant relative standard deviation of 0.088%.Conversely,the composite carbon material with sp^2 structure prepared at 700℃ is doped with nitrogen and gold nanoparticles,which exhibits good conductivity and electrocatalytic activity for uric acid oxidation.The uric acid sensor has linear response over a range of 1-150 μM and a limit of detection 0.1 μM.These results will provide new avenues where biological material will be the best start,which can be useful to target contradictory applications through molecular engineering at mesoscale. | Aniruddha BPatil Zhaohui Meng Ronghui Wu Liyun Ma Zijie Xu Chenyang Shi Wu Qiu Qiang Liu Yifan Zhang Youhui Lin Naibo Lin Xiang Yang Liu | 2020 | Nano-Micro Letters2020，12，9： | 3 |
| 7 | Gene expression profiles of the Southern house mosquito Culex quinquefasciatus during exposure to permethrin显示文摘 | William R. Reid Lee Zhang Youhui Gong Ting Li Nannan Liu | 2018 | Insect Science2018，25，3： | 3 |
| 8 | XB-SIM*:A Simulation Framework for Modeling and Exploration of ReRAM-Based CNN Acceleration Design显示文摘Resistive Random Access Memory(ReRAM)-based neural network accelerators have potential to surpass their digital counterparts in computational efficiency and performance.However,design of these accelerators faces a number of challenges including imperfections of the Re RAM device and a large amount of calculations required to accurately simulate the former.We present XB-SIM,a simulation framework for Re RAM-crossbar-based Convolutional Neural Network(CNN)accelerators.XB-SIM can be flexibly configured to simulate the accelerator’s structure and clock-driven behaviors at the architecture level.This framework also includes an Re RAM-aware Neural Network(NN)training algorithm and a CNN-oriented mapper to train an NN and map it onto the simulated design efficiently.Behavior of the simulator has been verified by the corresponding circuit simulation of a real chip.Furthermore,a batch processing mode of the massive calculations that are required to mimic the behavior of Re RAM-crossbar circuits is proposed to fully apply the computational concurrency of the mapping strategy.On CPU/GPGPU,this batch processing mode can improve the simulation speed by up to 5.02 or 34.29.Within this framework,comprehensive architectural exploration and end-to-end evaluation have been achieved,which provide some insights for systemic optimization. | Xiang Fei Youhui Zhang Weimin Zheng | 2021 | Tsinghua Science and Technology2021，26，3： | 2 |
| 9 | Automatic software deployment using user-level virtualization for cloud-eompu ting显示文摘 | Zhang Youhui LI Yanhua Zheng Weimin | 2011 | Future Generation Computer Systems2011，，8： | 1 |
| 10 | A user-space file system for on-demand legacy desktop software显示文摘Some user-level virtualization technologies have been used to convert legacy software (like the existing Windows desktop applications) into the on-demand software without any modification. To give the client a friendly and compatible method to access on-demand legacy software across the Internet, this paper presents a clientend file system for this usage case. It is a Windows user-space file system based on the cloud storage (where the on-demand software is stored), which converts the local file system accesses into remote visits. Quite a few optimizations are adopted and adjusted to suit the file-access-pattern of on-demand software, including the local cache, metadata/data/software prefetch and content-addressable storage (CAS), to decrease the number of remote visits and/or to overlap IO (input/output) operations with software execution. Detailed access-pattern analyses are also presented. This file system has been implemented and tests show that it is practical for much daily-used software-a local cache of limited size can provide up to 80% hit ratio and the corresponding overhead of running-time is about 37%. Owing to this method, on any compatible and networked computer, a user can use his/her personalized software conveniently although it does not exist on the local host. | ZHANG YouHui 1,2 , SU GeLin 1,2 & ZHENG WeiMin 1,2 1 Department of Computer Science and Technology, Tsinghua University, Beijing 100084, China 2 Tsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing 100084, China | 2011 | Science China(Information Sciences)2011，54，6： | 1 |
| 11 | Employing intelligence in object-based storage devices to provide attribute-based file access显示文摘Attribute-based file access has been regarded as a promising method to reach files based on their attributes rather than through the usual directory hierarchy,which is especially meaningful for locating the target among mass of files.However,because the attribute-based file system introduces higher performance overhead and is usually incompatible with existing file access interfaces,till now there are few practical attribute-based file systems.This paper designs a file system with attribute-based access methods and a compatible access interface,using object-based storage devices(OSD).In detail,this design fully utilizes existing features of the OSD standard(T10):object attributes and access methods are mapped into file attributes and the corresponding access interfaces respectively;collection-objects are used for fast indexing of user objects.Therefore,the attribute-based object organization can be achieved in OSD efficiently and the powerful processing capability of OSD is used to complete object-queries to speed up the performance.Moreover,the way to export the new accessing methods through file system with a compatible interface is also presented.Such a prototype has been implemented.Tests show that it introduces much less overheads than some existing attribute-based file systems,and the query performance is higher than its counterpart based on the block-level device. | ZHANG YouHui QIAN ZiQiang ZHENG WeiMin | 2013 | Science China(Information Sciences)2013，56，3： | 1 |
| 12 | Automatic software deployment using user-level virtualization for cloud-computing显示文摘 | Youhui Zhang Yanhua Li Weimin Zheng | 2013 | Future Generation Computer Systems2013，，1： | 1 |
| 13 | A GSH-responsive PET-based fluorescent probe for cancer cells imaging显示文摘An efficient PET-based probe,in which the ferrocene quencher and the naphthalimide fluorophore are linked by a disulfide bond,has been developed.This probe can be activated by GSH with fluorescence a turn-on response for blocking the PET process.In addition,it was successfully applied for distinguishing cancer cells from normal cells。 | Xue Li Huaying Wang Youhui Zhang Qianyong Cao Yong Chen | 2021 | Chinese Chemical Letters2021，32，4： | 1 |
| 14 | Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity显示文摘STING,an endoplasmic reticulum(ER)transmembrane protein,mediates innate immune activation upon cGAMP stimulation and is degraded through autophagy.Here,we report that activated STING could be transferred between cells to promote antitumor immunity,a process triggered by RAB22A-mediated non-canonical autophagy.Mechanistically,RAB22A engages PI4K2A to generate PI4P that recruits the Atg12–Atg5–Atg16L1 complex,inducing the formation of ER-derived RAB22A-mediated non-canonical autophagosome,in which STING activated by agonists or chemoradiotherapy is packaged.This RAB22A-induced autophagosome fuses with RAB22A-positive early endosome,generating a new organelle that we name Rafeesome(RAB22A-mediated non-canonical autophagosome fused with early endosome).Meanwhile,RAB22A inactivates RAB7 to suppress the fusion of Rafeesome with lysosome,thereby enabling the secretion of the inner vesicle of the autophagosome bearing activated STING as a new type of extracellular vesicle that we define as R-EV(RAB22A-induced extracellular vesicle).Activated STING-containing R-EVs induce IFNβrelease from recipient cells to the tumor microenvironment,promoting antitumor immunity.Consistently,RAB22A enhances the antitumor effect of the STING agonist diABZI in mice,and a high RAB22A level predicts good survival in nasopharyngeal cancer patients treated with chemoradiotherapy.Our findings reveal that Rafeesome regulates the intercellular transfer of activated STING to trigger and spread antitumor immunity,and that the inner vesicle of non-canonical autophagosome originated from ER is secreted as R-EV,providing a new perspective for understanding the intercellular communication of organelle membrane proteins. | Ying Gao Xueping Zheng Boyang Chang Yujie Lin Xiaodan Huang Wen Wang Shirong Ding Weixiang Zhan Shang Wang Beibei Xiao Lanqing Huo Youhui Yu Yilin Chen Run Gong Yuanzhong Wu Ruhua Zhang Li Zhong Xin Wang Qiuyan Chen Song Gao Zhengfan Jiang Denghui Wei Tiebang Kang | 2022 | Cell Research2022，32，12： | 1 |
| 15 | Using silk-derived magnetic carbon nanocomposites as highly efficient Nanozymes and electromagnetic absorbing agents显示文摘Functional carbon nanomaterials have become the stars of many active research fields,such as electronics,energy,catalysis,imaging,sensing and biomedicine.Herein,a facile and one-pot strategy for generating ferromagnetic nanoparticles loaded on N-doped carbon nanosheets(Fe-N-CNS)is presented by salt-assisted high-temperature carbonization of natural silk proteins.Due to their graphitic structures,N-doping and ferromagnetic nanoparticles(FeN_(x),FeO_(y),FeC_(z)),the silk-derived Fe-N-CNS can act as excellent mimics of both peroxidase and oxidase.Benefiting from the combined character of the graphene-like structures and enzyme-like activities,Fe-N-CNS can be further applied to highly efficient dye removal via synergistic adsorption and degradation.Meanwhile,the as-prepared Fe-N-CNS with intrinsic magnetism and electrical conductivity can also serve as an efficient electromagnetic wave absorption agent.The broadest effective absorption bandwidth(EAB)of as-obtained absorbing material yields a 6.73 GHz with 1 mm thickness,with a maximum reflection loss of-37.33 dB(11.41 GHz).The EAB can cover2~18 GHz with a tunable absorber thickness from 1.0 mm to 5.0 mm.Collectively,Fe-N-CNS,as a dualfunctional material,can tackle the aggravating environmental pollution issues of both dyes and electromagnetic waves. | Hao Wang Xianhui Zhang Yonghua Tang Weifeng Rong Jiachen Zhao Chaoyu Fan Zhisen Zhang Zhijun Sun Yun Yang Youhui Lin | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
| 16 | A Rational Design of Metal–Organic Framework Nanozyme with High‑Performance Copper Active Centers for Alleviating Chemical Corneal Burns显示文摘Metal–organic frameworks(MOFs)have attracted significant research interest in biomimetic catalysis.However,the modulation of the activity of MOFs by precisely tuning the coordination of metal nodes is still a significant challenge.Inspired by metalloenzymes with well-defined coordination structures,a series of MOFs containing halogen-coordinated copper nodes(Cu-X MOFs,X=Cl,Br,I)are employed to elucidate their structure–activity relationship.Intriguingly,experimental and theoretical results strongly support that precisely tuning the coordination of halogen atoms directly regulates the enzyme-like activities of Cu-X MOFs by influencing the spatial configuration and electronic structure of the Cu active center.The optimal Cu–Cl MOF exhibits excellent superoxide dismutase-like activity with a specific activity one order of magnitude higher than the reported Cu-based nanozymes.More importantly,by performing enzyme-mimicking catalysis,the Cu–Cl MOF nanozyme can significantly scavenge reactive oxygen species and alleviate oxidative stress,thus effectively relieving ocular chemical burns.Mechanistically,the antioxidant and antiapoptotic properties of Cu–Cl MOF are achieved by regulating the NRF2 and JNK or P38 MAPK pathways.Our work provides a novel way to refine MOF nanozymes by directly engineering the coordination microenvironment and,more significantly,demonstrating their potential therapeutic effect in ophthalmic disease. | Yonghua Tang Yi Han Jiachen Zhao Yufei Lv Chaoyu Fan Lan Zheng Zhisen Zhang Zuguo Liu Cheng Li Youhui Lin | 2023 | Nano-Micro Letters2023，15，8： | 0 |
| 17 | Size effect of layer thickness on stress fields due to interface core-spreading dislocation arrays in multilayers显示文摘The elastic fields due to interface dislocation arrays with spreading core in multilayers are derived analytically by means of the superposition of Green function of individual compact dislocations. The numerical results for Cu-Nb multilayers with dislocation arrays demonstrate that:(1) There exists a critical layer thickness(CLT) in describing interface shear stress. The maximum interface shear stress(MISS) increases with the layer thickness as it exceeds CLT;while the MISS decreases rapidly with the layer thickness as it is thinner than CLT.(2) Both the density and core width of interface dislocations have salient effect on the stress fields. The overall stress field is confined by high density of dislocations, and the stress singularity is released by core spreading.(3) A new dislocation prefers to glide in the middle region between the two adjacent interface dislocations by means of Peach-Kohler(P-K) force acting on the dislocation and elastic energy of dislocations. These findings would provide foundations to deepen the understanding of microscopic plastic deformation mechanisms and their related macroscopic mechanical properties of metallic anisotropic multilayers. | ZHANG YuHeng XU YouHui CHU HaiJian | 2020 | Science China(Technological Sciences)2020，63，2： | 0 |
| 18 | Towards“General Purpose”Brain-Inspired Computing System显示文摘Brain-inspired computing refers to computational models,methods,and systems,that are mainly inspired by the processing mode or structure of brain.A recent study proposed the concept of'neuromorphic completeness'and the corresponding system hierarchy,which is helpful to determine the capability boundary of brain-inspired computing system and to judge whether hardware and software of brain-inspired computing are compatible with each other.As a position paper,this article analyzes the existing brain-inspired chips design characteristics and the current so-called'general purpose'application development frameworks for brain-inspired computing,as well as introduces the background and the potential of this proposal.Further,some key features of this concept are presented through the comparison with the Turing completeness and approximate computation,and the analyses of the relationship with'general-purpose'brain-inspired computing systems(it means that computing systems can support all computable applications).In the end,a promising technical approach to realize such computing systems is introduced,as well as the on-going research and the work foundation.We believe that this work is conducive to the design of extensible neuromorphic complete hardware-primitives and the corresponding chips.On this basis,it is expected to gradually realize'general purpose'brain-inspired computing system,in order to take into account the functionality completeness and application efficiency. | Youhui Zhang Peng Qu Weimin Zheng | 2021 | Tsinghua Science and Technology2021，26，5： | 0 |
| 19 | Stereoselective synthesis of the 3,6-branched Fuziα-glucans up to 15-mer via a one-pot and convergent glycosylation strategy显示文摘A family of the 3,6-branched Fuziα-glucans including the pentasaccharide repeating unit as well as its di-and trimers were efficiently achieved via a one-pot and convergent glycosylation strategy.All the protectedα-glucans up to 15-mer were assembled with high yields and excellentα-stereoselectivity,which was secured by the synergisticα-directing effects of the Tol SCl/Ag OTf promotion system and the stericβ-facial shielding of bulky saccharide residues linked at the 6-O-position of glucosyl donors.Moreover,the 3,6-branched architecture of glycosyl donor was revealed to be more favorable for theα-selective glucosidation of primary hydroxyl group,especially in the case of large oligosaccharide acceptor.The structurally well-defined syntheticα-glucans would be useful for various biological studies. | Qingpeng Zhao Shihao Zhou Yue Wang Xiaoyu Yang Youhui Meng Yanxin Zhang Jian Gao | 2023 | Chinese Chemical Letters2023，34，6： | 0 |
| 20 | Stereodirecting Effects of 4-O-ortho-cyanobenzyl Ether on Chemical Glycosylation with Glucopyranosyl Donor显示文摘Here we report a systematic investigation on the stereodirecting effects of a 4-O-ortho-cyanobenzyl ether(oBCN)group with a glucopyranosyi donor.Experimental studies revealed that the glycosylation reactions using this donor generally show dual-stereoselectivities depending on the acceptor nucleophilicity,that is,glycosylation of weak nucleophile acceptors generally affordsα-glycosides specifically while glycosylation of strong nucleophile acceptors affords β-glycosides preferentially. | Han Zhang Aoxin Guo Youhui Meng Yue Wang Jian Gao Xue-Wei Liu | 2023 | Chinese Journal of Chemistry2023，41，6： | 0 |
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