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| 1 | Ulva prolifera green-tide outbreaks and their environmental impact in the Yellow Sea, China显示文摘The Ulva prolifera green tides in the Yellow Sea, China, which have been occurring since 2007, are a serious environmental problem attracting worldwide attention. Despite extensive research, the outbreak mechanisms have not been fully understood. Comprehensive analysis of anthropogenic and natural biotic and abiotic factors reveals that human activities, regional physicochemical conditions and algal physiological characteristics as well as ocean warming and biological interactions(with microorganism or other macroalgae) are closely related to the occurrence of green tides. Dynamics of these factors and their interactions could explain why green tides suddenly occurred in 2007 and decreased abruptly in 2017.Moreover, the consequence of green tides is serious. The decay of macroalgal biomass could result in hypoxia and acidification, possibly induce red tide and even have a long-lasting impact on coastal carbon cycles and the ecosystem. Accordingly, corresponding countermeasures have been proposed in our study for future reference in ecosystem management strategies and sustainable development policy. | Yongyu Zhang Peimin He Hongmei Li Gang Li Jihua Liu Fanglue Jiao Jianheng Zhang Yuanzi Huo Xiaoyong Shi Rongguo Su Naihao Ye Dongyan Liu Rencheng Yu Zongling Wang Mingjiang Zhou Nianzhi Jiao | 2019 | National Science Review2019，6，4： | 17 |
| 2 | Variability of soil organic carbon reservation capability between coastal salt marsh and riverside freshwater wetland in Chongming Dongtan and its microbial mechanism显示文摘Two representative zones in Chongming Dongtan which faced the Yangtze River and East China Sea respectively were selected to study the variability of soil organic carbon (SOC) reservation capability between coastal wetland and riverside wetland in the Chongming Dongtan wetland as well as its mechanism by analyzing soil characteristics and plant biomass.The results showed the SOC content of riverside wetland was only 48.61% (P=0.000 < 0.05) that of coastal wetland.As the organic matter inputs from plant litter of the coastal wetland and riverside wetland were approximately the same,the higher soil microbial respiration (SMR) of riverside wetland led to its lower SOC reservation capability.In the riverside wetland,the high soil microbial biomass,higher proportion of β-Proteobacteria,which have strong carbon metabolism activity and the existence of some specific aerobic heterotrophic bacteria such as Bacilli and uncultured Lactococcus,were the important reasons for the higher SMR compared to the coastal wetland.There were additional differences in soil physical and chemical characteristics between the coastal wetland and riverside wetlands.Path analysis of predominant bacteria and microbial biomass showed that soil salinity influenced β-Proteobacteria and microbial biomass most negatively among these physical and chemical factors.Therefore the low salinity of the riverside area was suitable for the growth of microorganisms,especially β-Proteobacteria and some specific bacteria,which led to the high SMR and low SOC reservation capability when compared to the coastal area. | Yu Hu Yanli Li Lei Wang Yushu Tang Jinhai Chen Xiaohua Fu Yiquan Le Jihua Wu | 2012 | Journal of Environmental Sciences2012，24，6： | 7 |
| 3 | A high-quality genome assembly of Morinda officinalis,a famous native southern herb in the Lingnan region of southern China显示文摘Morinda officinalis is a well-known medicinal and edible plant that is widely cultivated in the Lingnan region of southern China.Its dried roots(called bajitian in traditional Chinese medicine)are broadly used to treat various diseases,such as impotence and rheumatism.Here,we report a high-quality chromosome-scale genome assembly of M.officinalis using Nanopore single-molecule sequencing and Hi-C technology.The assembled genome size was 484.85Mb with a scaffold N50 of 40.97 Mb,and 90.77%of the assembled sequences were anchored on eleven pseudochromosomes.The genome includes 27,698 protein-coding genes,and most of the assemblies are repetitive sequences.Genome evolution analysis revealed that M.officinalis underwent core eudicotγgenome triplication events but no recent whole-genome duplication(WGD).Likewise,comparative genomic analysis showed no large-scale structural variation after species divergence between M.officinalis and Coffea canephora.Moreover,gene family analysis indicated that gene families associated with plant–pathogen interactions and sugar metabolism were significantly expanded in M.officinalis.Furthermore,we identified many candidate genes involved in the biosynthesis of major active components such as anthraquinones,iridoids and polysaccharides.In addition,we also found that the DHQS,GGPPS,TPS-Clin,TPS04,sacA,and UGDH gene families—which include the critical genes for active component biosynthesis—were expanded in M.officinalis.This study provides a valuable resource for understanding M.officinalis genome evolution and active component biosynthesis.This work will facilitate genetic improvement and molecular breeding of this commercially important plant. | Jihua Wang Shiqiang Xu Yu Mei Shike Cai Yan Gu Minyang Sun Zhan Liang Yong Xiao Muqing Zhang Shaohai Yang | 2021 | Horticulture Research2021，8，1： | 4 |
| 4 | Numerical simulation of seepage flow field in groundwater source heat pump system and its influence on temperature field显示文摘Energy utilization in the aquifers is a new technology closely related to development of heat pump technique. It is significant for the flow distribution to be predicted in the aquifer surrounding the Groundwater Source Heat Pump System (GSHPS). The authors presented a new concept of 'flow transfixion' by analyzing general features of aquifers, and then discussed interaction of the flow transfixion with the heat transfixion, which has practical significance to projects. A numerical model of groundwater flow was established based on the basic tenets of water-heat transferring in the aquifer. On this basis the flow field and the temperature field of GSHPS for a site in Shenyang City were numerically simulated. The basis of the flow transfixion was obtained; it was discussed for the influence of the flow transfixion on the heat transfixion. To a certain extent, the study offers some reference for the projects' design of GSHP in the studied area. | Jihua HU Yanjun ZHANG Danyan DU Gang WU Ziwang YU Chen WANG Fuquan NI | 2008 | Global Geology2008，11，3： | 4 |
| 5 | Gap-free genome assembly and comparative analysis reveal the evolution and anthocyanin accumulation mechanism of Rhodomyrtus tomentosa显示文摘Rhodomyrtus tomentosa is an important f leshy-fruited tree and a well-known medicinal plant of the Myrtaceae family that is widely cultivated in tropical and subtropical areas of the world.However,studies on the evolution and genomic breeding of R.tomentosa were hindered by the lack of a reference genome.Here,we presented a chromosome-level gap-free T2T genome assembly of R.tomentosa using PacBio and ONT long read sequencing.We assembled the genome with size of 470.35 Mb and contig N50 of∼43.80 Mb with 11 pseudochromosomes.A total of 33382 genes and 239.31 Mb of repetitive sequences were annotated in this genome.Phylogenetic analysis elucidated the independent evolution of R.tomentosa starting from 14.37MYA and shared a recent WGD event with other Myrtaceae species.We identified four major compounds of anthocyanins and their synthetic pathways in R.tomentosa.Comparative genomic and gene expression analysis suggested the coloring and high anthocyanin accumulation in R.tomentosa tends to be determined by the activation of anthocyanin synthesis pathway.The positive selection and up-regulation of MYB transcription factors were the implicit factors in this process.The copy number increase of downstream anthocyanin transport-related OMT and GST gene were also detected in R.tomentosa.Expression analysis and pathway identification enriched the importance of starch degradation,response to stimuli,effect of hormones,and cell wall metabolism during the f leshy fruit development in Myrtaceae.Our genome assembly provided a foundation for investigating the origins and differentiation of Myrtaceae species and accelerated the genetic improvement of R.tomentosa. | angping Li Shiqiang Xu Zitong Xiao Jingming Wang Yu Mei Haifei Hu Jingyu Li Jieying Liu Zhuangwei Hou Junliang Zhao Shaohai Yang Jihua Wang | 2023 | Horticulture Research2023，10，3： | 3 |
| 6 | A predicted NEDD8 conjugating enzyme gene identified as a Capsicum candidate Rf gene using bulk segregant RNA sequencing显示文摘Cytoplasmic male sterility(CMS)is an important tool for producing F1 hybrids,which can exhibit heterosis.The companion system,restorer-of-fertility(Rf),is poorly understood at the molecular level and would be valuable in producing restorer lines for hybrid seed production.The identity of the Rf gene in Capsicum(pepper)is currently unclear.In this study,using bulked segregant RNA sequencing(BSR-seq),a strong candidate Rf gene,Capana06g002866,which is annotated as a NEDD8 conjugating enzyme E2,was identified.Capana06g002866 has an ORF of 555 bp in length encoding 184 amino acids;it can be cloned from F1 plants from the hybridization of the CMS line 8A and restorer line R1 but is not found in CMS line 8A.With qRT-PCR validation,Capana06g002866 was found to be upregulated in restorer accessions compared to sterile accessions.The relative expression in flower buds increased with the developmental stage in F1 plants,while the expression was very low in all flower bud stages of the CMS lines.These results provide new insights into the Rf gene in pepper and will be useful for other crops utilizing the CMS system. | Bingqiang Wei Paul W.Bosland Zhenghai Zhang Yongfu Wang Gaoyuan Zhang Lanlan Wang Jihua Yu | 2020 | Horticulture Research2020，7，1： | 3 |
| 7 | Excitation-emission matrix (EEM) fluorescence spectroscopy for characterization of organic matter in membrane bioreactors: Principles, methods and applications显示文摘The membrane bioreactor(MBR)technology is a rising star for wastewater treatment.The pollutant elimination and membrane fouling performances of MBRs are essentially related to the dissolved organic matter(DOM)in the system.Three-dimensional excitation-emission matrix(3D-EEM)fluorescence spectroscopy,a powerful tool for the rapid and sensitive characterization of DOM,has been extensively applied in MBR studies;however,only a limited portion of the EEM fingerprinting information was utilized.This paper revisits the principles and methods of fluorescence EEM,and reviews the recent progress in applying EEM to characterize DOM in MBR studies.We systematically introduced the information extracted from EEM by considering the fluorescence peak location/intensity,wavelength regional distribution,and spectral deconvolution(giving fluorescent component loadings/scores),and discussed how to use the information to interpret the chemical compositions,physiochemical properties,biological activities,membrane retention/fouling behaviors,and migration/transformation fates of DOM in MBR systems.In addition to conventional EEM indicators,novel fluorescent parameters are summarized for potential use,including quantum yield,Stokes shift,excited energy state,and fluorescence lifetime.The current limitations of EEM-based DOM characterization are also discussed,with possible measures proposed to improve applications in MBR monitoring. | Jinlan Yu Kang Xiao Wenchao Xue Yue-xiao Shen Jihua Tan Shuai Liang Yanfen Wang Xia Huang | 2020 | Frontiers of Environmental Science & Engineering2020，14，2： | 3 |
| 8 | Development of FGF-2-loaded electrospun waterborne polyurethane fibrous membranes for bone regeneration显示文摘Guided bone regeneration(G BR)membrane has been used to improve functional outcomes for periodontal regener ation.However,few studies have focused on the biomimetic membrane mim-icking the vascularization of the periodontal membrane.This study aimed to fabricate waterborne polyurethane(WPU)fibrous membranes loaJed fibroblast growth factor-2(FGF-2)via emulsion electrospinning,which can promote regeneration of periodontal tissue via the vascularization of the biomimetic GBR membrane.A biodegradable WPU was synthesized by using lysine and dime-thylpropionic acid as chain extenders according to the rule of green chemical synthesis technology.The WPU fibers with FGF-2 was fabricated via emulsion electrospinning.The results confirmed that controlled properties of the fibrous membrane had been achieved with controlled degradation,suitable mech anical properties and sustained release of the factor.The immunohistochemical expression of angiogenic-related factors was positive,meaning that FGF-2 loaded in fibers can sig-nificantly promote cell vasculariz ation.The fiber scaffold loaded FGF-2 has the potential to be used as a functional GBR membrane to promote the formation of extraosseous blood vessels during periodontal repairing. | Chi Zhang Jianxiong Wang Yujie Xie Li Wang Lishi Yang Jihua Yu Akira Miyamoto Fuhua Sun | 2021 | Regenerative Biomaterials2021，8，1： | 3 |
| 9 | Comparative transcriptome analysis of sweet corn seedlings under low-temperature stress显示文摘Stress induced by low temperature, which represents a widespread environmental factor,strongly affects maize growth and yield. However, the physiological characteristics and molecular regulatory mechanisms of maize seedlings in response to cold remain poorly understood. In this study, using RNA-seq, we investigated the transcriptome profiles of two sweet com inbred lines, 'Richao'(RC) and C5, under cold stress. A total of 357 and 455 differentially expressed genes(DEGs) were identified in the RC and C5 lines, respectively, 94 DEGs were detected as common DEGs related to cold response in both genotypes, and a total of 589 DEGs were detected as cold tolerance-associated genes. By combining protein function clustering analysis and significantly enriched Gene Ontology(GO) terms analysis,we suggest that transcription factors may play a dominating role in the cold stress response and tolerance of sweet com. Furthermore, 74 differentially expressed transcription factors were identified, of those many genes involved in the metabolism and regulation of hormones. These results expand our understanding of the complex mechanisms involved in chilling tolerance in maize, and provide a set of candidate genes for further genetic analyses. | Jihua Mao Yongtao Yu Jing Yang Gaoke Li Chunyan Li Xitao Qi Tianxiang Wen Jianguang Hu | 2017 | The Crop Journal2017，5，5： | 2 |
| 10 | Binary tree slotted aloha for passive RFID tag anti-collision显示文摘 | WU Haifeng ZENG Yu FENG Jihua | 2013 | IEEE Transactions on Parallel and Distributed Systems2013，24，1： | 1 |
| 11 | Arthroscopic Anterior Cruciate Ligament Reconstruction With at Least 2.5 Years’ Follow-up Comparing Hamstring Tendon Autograft and Irradiated Allograft显示文摘 | Kang Sun Jihua Zhang Yan Wang Changsuo Xia Cailong Zhang Tengbo Yu Shaoqi Tian | 2011 | Arthroscopy: The Journal of Arthroscopic and Related Surgery2011，，： | 1 |
| 12 | Effects of minor Gd addition on microstructures and mechanical properties of the high strain-rate rolled Mg-Zn-Zr alloys 显示文摘 | Yu Haiyang Yah Hongge Chen Jihua | 2014 | Journal of Alloys and Compounds2014，586，： | 1 |
| 13 | 显示文摘 | Guo Qingfeng Hao Jihua | 2000 | Desalination2000，129，： | 1 |
| 14 | Characteristics of atmospheric non-methane hydrocarbons during high PM 10 episodes and normal days in Foshan, China显示文摘 | Songjun Guo Jihua Tan Yongliang Ma Fumo Yang Yongchan Yu Jiewen Wang | 2011 | Atmospheric Research2011，，3： | 1 |
| 15 | Cloning, characterization, and transformation of the phosphoethanolamine N-methyltransferase gene (ZmPEAMT1) in maize (Zea mays L.)显示文摘 | Suowei Wu Zhanwang Yu Fengge Wang Weihua Li Chunjiang Ye Jun Li Jihua Tang Junqiang Ding Jiuran Zhao Bin Wang | 2007 | Molecular Biotechnology2007，，2： | 1 |
| 16 | Effect of Ion Impinging on the Microstructure and Field Emission of Carbon Nanotubes显示文摘 | Zhang Jihua Wang Xi Yu Weidong | 2003 | Solid State Communications2003，127，： | 1 |
| 17 | Recovery of acetic acid from dilute wastewater by means of bipolar membrane electrodialysis显示文摘 | Yu Lixin Guo Qingfeng Hao Jihua | 2000 | Desolination2000，129，3： | 1 |
| 18 | Effect of Ion Impinging on the Microstructure and Field Emission of Carbon Nanotubes显示文摘 | Zhang Jihua Wang Xi Yu Weidong | 2003 | Solid State Communications2003，127，： | 1 |
| 19 | Effects of minor Gd addition on microstructures and mechanical properties of the high strain-rate rolled Mg-Zn-Zr alloys显示文摘 | Yu Haiyang Yan Hongge Chen Jihua | 2014 | Materials Characterization Journal of Alloys and Compounds2014，586，： | 1 |
| 20 | Recovery of acetic acid from dilute wasterwater by means of bipolar membrane electrodialysis 显示文摘 | YU Lixin GUO Qingfeng HAO Jihua | 2000 | Desalination2000，129，3： | 1 |
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