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| 1 | Molecular Cytogenetic Characterization of a Wheat-Leymus mollis 3D(3Ns) Substitution Line with Resistance to Leaf Rust显示文摘Leymus mollis(Trin.)Pilger(NsNsXmXm,2n?28),a wild relative of common wheat,possesses many potentially valuable traits that could be transferred to common wheat during breeding programs.In this study,the karyotypic constitution of a wheat e L.mollis 3D(3Ns#1)disomic substitution line isolated from the F5progeny of octoploid Tritileymus M842-16 Triticum durum cv.D4286,which was designated as 10DM57,was determined using genomic in situ hybridization(GISH),fluorescent in situ hybridization(FISH),SSR markers,and ESTSTS markers.Screening of mitosis and meiosis showed that 10DM57 had a chromosome karyotype of 2n?42?21II.GISH indicated that10DM57 was a line with 40 chromosomes from wheat and two of the Ns chromosomes from L.mollis,which formed a ring bivalent in pollen mother cells at metaphase I.FISH analysis showed that the chromosome 3D may be replaced by 3Ns#1 in 10DM57.DNA markers,including SSR and EST-STS primers,showed that the pair of wheat chromosome 3D in 10DM57 was substituted by the pair of chromosome 3Ns#1from L.mollis.Evaluation of the agronomic traits showed that,compared with its common wheat relative 7182,10DM57 was resistant to leaf rust while the spike length and number of spikes per plant were improved significantly,which correlated with a higher wheat yield.The new germplasm,10DM57,could be exploited as an intermediate material in wheat genetic and breeding programs. | Yuhui Pang Xinhong Chen Jixin Zhao Wanli Du Xueni Cheng Jun Wu Yanli Li Liangming Wang Jing Wang Qunhui Yang | 2014 | Journal of Genetics and Genomics2014，41，4： | 6 |
| 2 | Development and identification of a dwarf wheat-Leymus mollis double substitution line with resistance to yellow rust and Fusarium head blight显示文摘Leymus mollis (Trin.) Pilger (2n = 4x = 28, NsNsXmXm,), a wild relative of common wheat, possesses many potentially valuable traits for genetic improvement of wheat, including strong, short stems, long spikes with numerous spikelets, tolerance to drought and cold stresses, and resistance to many fungal and bacterial diseases. In the present study, a wheat-L. mollis double substitution line DM96 was selected from a F6 progeny of a cross between M842-16 (an octoploid Tritileymus line) and D4286 (a Triticum durum line) using genomic in situ hybridization (GISH), simple sequence repeat (SSR) markers, and expressed sequence tagged sequence site (EST-STS) markers. Chromosome analysis at mitosis and meiosis showed that DM96 had a chromosome constitution of 2n = 42 = 21II. GISH analysis indicated that DM96 carried 38 chromosomes from wheat and two homologous pairs of Ns chromosomes from L. mollis. Fluorescent in situ hybridization (FISH) showed that chromosomes 2Ns and 3Ns from L. mollis had replaced wheat chromosomes 2D and 3D in DM96, which was confirmed by SSR and STS markers. The newly developed substitution line DM96 has shorter height, longer spikes and more kernels than its parents and showed high resistance to stripe rust and Fusarium head blight (FHB). Thus, this line is a new bridge material for the production of useful translocation lines for wheat genetic research and genetic improvement of wheat yield and disease resistance in breeding programs. | Jixin Zhao Yang Liu Xueni Cheng Yuhui Pang Jiachuang Li Zhenqi Su Jun Wu Qunhui Yang Guihua Bai Xinhong Chen | 2019 | The Crop Journal2019，7，4： | 2 |
| 3 | Towards ultrastrong and ductile medium-entropy alloy through dual-phase ultrafine-grained architecture显示文摘Advanced materials with superior comprehensive mechanical properties are strongly desired,but it has long been a challenge to achieve high ductility in high-strength materials.Here,we proposed a new V 0.5 Cr 0.5 CoNi medium-entropy alloy(MEA)with a face-centered cubic/hexagonal close-packed(FCC/HCP)dual-phase ultrafine-grained(UFG)architecture containing stacking faults(SFs)and local chemical order(LCO)in HCP solid solution,to obtain an ultrahigh yield strength of 1476 MPa and uniform elongation of 13.2%at ambient temperature.The ultrahigh yield strength originates mainly from fine grain strength-ening of the UFG FCC matrix and HCP second-phase strengthening assisted by the SFs and LCO inside,whereas the large ductility correlates to the superior ability of the UFG FCC matrix to storage disloca-tions and the function of deformation-induced SFs in the vicinity of the FCC/HCP boundary to eliminate the stress concentration.This work provides new guidance by engineering novel composition and stable UFG structure for upgrading the mechanical properties of metallic materials. | Zhen Chen Hongbo Xie Haile Yan Xueyong Pang Yuhui Wang Guilin Wu Lijun Zhang Hu Tang Bo Gao Bo Yang Yanzhong Tian Huiyang Gou Gaowu Qin | 2022 | Journal of Materials Science & Technology2022，，31： | 1 |
| 4 | Expression of obesity?related miR?1908 in human adipocytes is regulatedby adipokines, free fatty acids and hormones显示文摘 | Xinye Jiang Lei Yang Lingxia Pang Ling Chen Xirong Guo Chenbo Ji Chunmei Shi Yuhui Ni | 2014 | Molecular Medicine Reports2014，，2： | 1 |
| 5 | Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing显示文摘Wheat leaf rust is a prevalent foliar disease in wheat worldwide. Growing resistant cultivars is an effective strategy to minimize the impact of leaf rust on yield and grain quality. Lr42 is a leaf rust resistance gene identified from Aegilops tauschii and is still effective against current predominant leaf rust races in the United States and many other countries. In this study, we developed diagnostic DNA markers for Lr42 using the sequence polymorphisms of a differentially expressed gene(TaRPM1) encoding a putative NBARC protein in the Lr42 candidate region identified by RNA-sequencing of two near-isogenic lines contrasting in Lr42 alleles. Markers were designed based on a deletion mutation and a single nucleotide polymorphism(SNP) in the gene. Haplotype analyses of the newly developed markers in the three diversity panels demonstrated that they are diagnostic for Lr42, and superior to previously used markers in selection accuracy. These markers have the advantages of low cost and easy assay, and they are suitable for marker-assisted selection in breeding programs with either high-or low-throughput marker screening facilities. | Yang Liu Hui Chen Chunxin Li Lirong Zhang Mingqin Shao Yuhui Pang Xiangyang Xu Guihua Bai | 2021 | The Crop Journal2021，9，6： | 0 |
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